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Optimization of cultivation conditions for pullulan production from Aureobasidium pullulans MR by response surface methodology (반응표면분석법을 이용한 Aureobasidium pullulans MR의 풀루란 생산을 위한 배양 조건 최적화)

  • Jo, Hye-Mi;Kim, Ye-Jin;Yoo, Sang-Ho;Kim, Chang-Mu;Kim, KyeWon;Park, Cheon-Seok
    • Korean Journal of Food Science and Technology
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    • v.53 no.2
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    • pp.195-203
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    • 2021
  • Aureobasidium pullulans, a black yeast, produces pullulan, a linear α-glucan composed of maltotriose repeating units linked by α(1→6)-glycosidic linkages. Pullulan can be widely used in food, cosmetic, and biotechnology industries. In this study, we isolated eight strains of A. pullulans from Forsythia koreana, Magnolia kobus DC., Spiraea prunifolia var. simpliciflora, Cornus officinalis, Cerasus, and Hippophae rhamnoides. Among them, A. pullulans MR was selected as the best pullulan producer. The effects of a carbon source, a nitrogen source, and pH on pullulan production were examined. The optimal cultivation conditions for pullulan production by A. pullulans MR were determined by response surface methodology as 15% sucrose, 0.4% soy peptone, and an initial pH of 7 at 26℃. Under these conditions, the predicted pullulan production was 47.6 g/L, which was very close to the experimental data (48.9 g/L).

Developing a Practical Course Model for the Personality Education in a Theological University (신학대학교에서 성품 교육을 위한 실천적 코스 모델 개발)

  • Yi, Miah
    • Journal of Christian Education in Korea
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    • v.66
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    • pp.75-112
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    • 2021
  • This study is purposed to prepare students in theological universities so that they contribute to their faith community as well as the broader social community in future through developing a practical course model for education of character formation. Facing the challenge of a high-tech revolution being progressed globally in this period, theological seminaries as the Christian-affiliated institute of higher education see the necessity to nurture their learners' competency of holistic personality comprehending cognitive, affective, behavioral, and more spiritual maturity as well as specialty in each subject matter. The holistic character capability of seminarians is closely related not only to their own self-realization, but also social community's well-being. This research had the following content and results. Chapter 1 brought up a subject to educational needs of the holistic character formation in the theological seminaries to meet the rapidly changing times. Chapter 2 considered the importance of character formation in the curriculum of the universities and theological seminaries in the times of high-tech revolution through the literature reviews related to. Chapter 3 clarified the elements of character education to be placed emphasis on theological seminaries and established the way of course planning. Chapter 4, using a course of Christian social education for illustration, described in order the whole process of course planning comprehending the need of planning, description of course concept, establishment and definition of learning outcomes, organization of learning experience(course content), teaching-learning activities, evaluation of learning outcome, and course improvement, which was visualized with the chart of the process of course design. Also, it provided a practical illustration of the conceptual map, an outline of content and an organization of units, and scope-sequence chart. Finally, chapter 5 gave an syllabus example in an integrative and digestive form as the course plan to be distributed to the students, which is the final result produced through the whole process of course planning. This research is intended not to present a overall curriculum planning of the university-level in the theological seminaries, but to develop a course-level curriculum focused on the formation of character. The author hope that this study would be used as a guideline in developing a course model to nurture the capability of the learners' holistic character.

Provenance of the Sediments of the Araon Mound in the Chukchi Sea, Arctic Ocean (북극 척치해 아라온 마운드 퇴적물의 기원지에 관한 연구)

  • Jang, JeongKyu;Koo, HyoJin;Cho, HyenGoo
    • Korean Journal of Mineralogy and Petrology
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    • v.34 no.1
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    • pp.15-29
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    • 2021
  • In the Arctic Ocean, the distribution of sea ice and ice sheets changes as climate changes. Because the distribution of ice cover influences the mineral composition of marine sediments, studying marine sediments transported by sea ice or iceberg is very important to understand the global climate change. This study analyzes marine sediment samples collected from the Arctic Ocean and infers the provenance of the sediments to reconstruct the paleoenvironment changes of the western Arctic. The analyzed samples include four gravity cores collected from the Araon mound in the Chukchi Plateau and one gravity core collected from the slope between the Araon mounds. The core sediments were brown, gray, and greenish gray, each of which corresponds to the characteristic color of sediments deposited during the interglacial/glacial cycle in the western Arctic Ocean. We divide the core sediments into three units based on the analysis of bulk mineral composition, clay mineral composition, and Ice Rafted Debris (IRD) as well as comparison with previous study results. Unit 3 sediments, deposited during the last glacial maximum, were transported by sea ice and currents after the sediments of the Kolyma and Indigirka Rivers were deposited on the continental shelf of the East Siberian Sea. Unit 2 sediments, deposited during the deglacial period, were from the Kolyma and Indigirka Rivers flowing into the East Siberian Sea as well as from the Mackenzie River and the Canadian Archipelago flowing into the Beaufort Sea. Unit 2 sediments also contained an extensive amount of IRD, which originated from the melted Laurentide Ice Sheet. During the interglacial stage, fine-grained sediments of Unit 1 were transported by sea ice and currents from Northern Canada and the East Siberian Sea, but coarse-grained sediments were derived by sea ice from the Canadian Archipelago.

Development of A Material Flow Model for Predicting Nano-TiO2 Particles Removal Efficiency in a WWTP (하수처리장 내 나노 TiO2 입자 제거효율 예측을 위한 물질흐름모델 개발)

  • Ban, Min Jeong;Lee, Dong Hoon;Shin, Sangwook;Lee, Byung-Tae;Hwang, Yu Sik;Kim, Keugtae;Kang, Joo-Hyon
    • Journal of Wetlands Research
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    • v.24 no.4
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    • pp.345-353
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    • 2022
  • A wastewater treatment plant (WWTP) is a major gateway for the engineered nano-particles (ENPs) entering the water bodies. However existing studies have reported that many WWTPs exceed the No Observed Effective Concentration (NOEC) for ENPs in the effluent and thus they need to be designed or operated to more effectively control ENPs. Understanding and predicting ENPs behaviors in the unit and \the whole process of a WWTP should be the key first step to develop strategies for controlling ENPs using a WWTP. This study aims to provide a modeling tool for predicting behaviors and removal efficiencies of ENPs in a WWTP associated with process characteristics and major operating conditions. In the developed model, four unit processes for water treatment (primary clarifier, bioreactor, secondary clarifier, and tertiary treatment unit) were considered. Additionally the model simulates the sludge treatment system as a single process that integrates multiple unit processes including thickeners, digesters, and dewatering units. The simulated ENP was nano-sized TiO2, (nano-TiO2) assuming that its behavior in a WWTP is dominated by the attachment with suspendid solids (SS), while dissolution and transformation are insignificant. The attachment mechanism of nano-TiO2 to SS was incorporated into the model equations using the apparent solid-liquid partition coefficient (Kd) under the equilibrium assumption between solid and liquid phase, and a steady state condition of nano-TiO2 was assumed. Furthermore, an MS Excel-based user interface was developed to provide user-friendly environment for the nano-TiO2 removal efficiency calculations. Using the developed model, a preliminary simulation was conducted to examine how the solid retention time (SRT), a major operating variable affects the removal efficiency of nano-TiO2 particles in a WWTP.

The Study on the Lowest Limit Time of the Tending of Red Pine (Pinus densiflora) Forest for the Control of Pine Sawyer (Monochamus alternatus) (솔수염하늘소 제어를 위한 소나무림 숲가꾸기의 하한(下限)시기 구명)

  • Jeon, Kwon-Seok;Park, Nam-Chang;Yoon, Hee-Tak;Hong, Sung-Cheon
    • Journal of Korean Society of Forest Science
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    • v.100 no.3
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    • pp.352-358
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    • 2011
  • Field Cage plots ($1m{\times}1m{\times}1m$) were established (7 units) to find the lowest limit time about the tending of red pine forest (Pinus densiflora) which can no longer be used as a habitat by Monochamus alternatus, vector insect of pine wilt disease at the experimental forest of the southern forest research center of the Korea forest research institute in February in 2010. Thinning slashes (length, 1 m; diameter, 5~10 cm) tended at the different times were put in cages, and 4~6 couples of adult M. alternatus were put into each the cage in June. Presence or absence the larval entrance holes and larval were determined in November in 2010. Incase of the combination 24, 18, 12 and 6-month-old thinning slashes from thinning times to the time of adult emergence inside a single cage, larval entrance holes were found in the 6-month-old and 12-month-old thinning slashes but larvae were found only in the 6-month-old thinning slashes (treatment 1). In case of the combination 24, 18, 15 and 12-month-old thinning slashes inside a single cage, larval entrance holes were found in the 15-month-old and 12-month-old thinning slashes but larvae were found only in the 12-month-old (treatment 2). When 24, 18, 15, 12 and 6-month-old thinning slashes with treated dry and humid condition were put separately inside each cage, larval entrance holes were found in the 18, 15, 12, 6-month-old thinning slashes without the relation of the dry and humid conditions. But larvae were found in the 15, 12, 6-month-old thinning slashes in the dry conditions and only in the 6-month-old thinning slashes in the humid conditions. Results indicated the lowest limit time which can no longer be used as a habitat by M. alternatus is before 24 month from the time of adult emergence.

Distribution Types of the Relict Conifer Community and the Approach for the Ecological Management in Ulleung-Island (울릉도에 자생하는 침엽수류 유존군락의 분포유형과 생태적 관리방안에 대한 연구)

  • Cho, Hyun-Je;Lee, Jung-Hyo;Choo, Yeon-Sik;Hong, Sung-Cheon
    • Journal of Korean Society of Forest Science
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    • v.100 no.1
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    • pp.95-104
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    • 2011
  • Distribution types of native conifers (Juniperus chinensis, Pinus parviflora, Tusga sieboldii and Taxus cuspidata var. latifolia) were studied by phytosociological investigation and ZM method in Ulleung Island, South Korea. Two main types were divided maritime vegetation (Juniperus chinensis forest) and mountain vegetation (Taxus cuspidata var. latifolia forest and Pinus parviflora-Tusga sieboldii forest). The former was divided into sea cliff distribution (J-SC) and sea ridge distribution (J-SR) type. The latter was classified 7 distribution types; Taxus cuspidata var. latifolia forest was rock distribution (Ta-R) and mountain slope distribution (Ta-MS) type, and Pinus parviflora-Tusga sieboldii forest was rock distribution (P T-R), upper and ridge distribution (P T-UR, 3 units sub-types:1sub, 2sub, 3sub), and Mountain slope distribution (P T-MS) type. It was considered that J-SC, Ta- R, and P T-R were maintained by topographic climax, but J-SR, Ta-MS, P T-UR and P T-MS were the process of vegetation succession. Distribution types of topographic climax are entrusted to process of vegetation succession. Types in the process of vegetation succession will be needed tending of forest to promote saplings growth and seedlings germination. Especially in order to restore Tsuga sieboldii forest should be afforest and make forest gap because It is mid shade tolerant tree and purity percentage of its seed is 1~2%. It was considered that the composition of group mixture forest constituted Pinus parviflora, Tsuga sieboldii, Taxus cuspidata, Camellia japonica, Machilus thunbergii and Acer okamotoanum, etc. will be able to restore native vegetation, after take the form of forest gap by strong thinning and pruning of Pinus thunbergii forest.

The Palaces Weoldae(月臺) structure, in the latter half period of Joseon, and related rituals (조선후기 궁궐의 전각(殿閣) 월대(月臺)와 의례 - <동궐도>와 <서궐도안>을 대상으로 -)

  • Lee, Hyun-jin;Son, shin-young
    • (The)Study of the Eastern Classic
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    • no.67
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    • pp.379-418
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    • 2017
  • Weoldae -which we can find inside Joseon palaces- is usually erected in front of important structures including the Main Hall(Jeongjeon, 正殿). There is no clear definition of its nature that we can find in official law codes or dynastic ritual manuals, and such records do not specify what kind of rules should be observed in creating them either. In illustrations of palaces such as and , Weoldae can be seen at structures such as the Main "Jeongjeon" Hall(正殿), Royal office(Pyeonjeon, 便殿), Royal Quarters(Jeongchim, 正寢), Queen's bedroom(Chimjeon, 寢殿), Quarters of the Crown Prince(Dong'gung, 東宮), and other structures. All these structures equipped with a Weoldae were related to special figures inside the royal family such as the king, the queen, the crown prince, and the crown-prince' son. These figures were literally above the law, and as such their treatment could not be defined by law. And these spaces were where they worked or rested while they lived, and where their posthumous tablets(Shinju, 神主) or portraits(Eojin, 御眞) were enshrined after they died. (When such spaces were used for latter purposes, they were designated either as 'Honjeon[魂殿, chamber of the tablet]' or 'Jinjeon[眞殿, hall of portrait']). Joseon was a Confucian dynasty with a strict social stratification system, and the palace structures reflected such atmosphere as well. We can see that structures described with Weoldae in and were structures which were more important than others that did not have a Weoldae. Among structures with Weoldae, the place which hosted most of the dynastic rituals was the Main Hall. In this Main Hall, the King swore an oath himself, passed the incense(香) himself during memorial services, and observed honoring ceremonies[Manbae-rye(望拜禮)] at the "Hwangdan"(皇壇) altar which was built to commemorate the three Ming Emperors. The so-called "Two Palaces[兩闕]" of Joseon shared a unique relationship in terms of their own Weoldae units and the rituals that were held there. In the early half of the Joseon dynasty period, Gyeongbok-gung(景福宮) and Dong'gweol(東闕) constituted the "Two palaces," but after the war with the Japanese in the 1590s during which the Gyeongbok-gung palace was incinerated, Dong'gweol and Seogweol(西闕) came to newly form the "Two palaces" instead. Meanwhile, Changdeok-gung(昌德宮) became the main palace[法宮], replacing the previous one which had been Gyeongbok-gung. In general, when a king moved to another palace, the ancestral tablets in the Honjeon chamber or the portrait in the Jinjeon hall would accompany him as well. Their presence would be established within the new palace. But king Yeongjo was an exception from that practice. Even after he moved to the Gyeong'hi-gung(慶熙宮) palace, he continued to pay visit to the Jinjeon Hall at Changdeok-gung. While he was positioned inside Gyeong'hi-gung, he did not manage the palace with Gyeonghi-gung as its sole center. He tried to manage other palaces like Changdeok-gung and Chang'gyeong-gung(昌慶宮) as well, and as organically as possible.

Evaluation of Regional Flowering Phenological Models in Niitaka Pear by Temperature Patterns (경과기온 양상에 따른 신고 배의 지역별 개화예측모델 평가)

  • Kim, Jin-Hee;Yun, Eun-jeong;Kim, Dae-jun;Kang, DaeGyoon;Seo, Bo Hun;Shim, Kyo-Moon
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.22 no.4
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    • pp.268-278
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    • 2020
  • Flowering time has been put forward due to the recent abnormally warm winter, which often caused damages of flower buds by late frosts persistently. In the present study, cumulative chill unit and cumulative heat unit of Niitaka pear, which are required for releasing the endogenous dormancy and for flowering after breaking dormancy, respectively, were compared between flowering time prediction models used in South K orea. Observation weather data were collected at eight locations for the recent three years from 2018-2020. The dates of full bloom were also collected to determine the confidence level of models including DVR, mDVR and CD models. It was found that mDVR model tended to have smaller values (8.4%) of the coefficient of variation (cv) of chill units than any other models. The CD model tended to have a low value of cv (17.5%) for calculation of heat unit required to reach flowering after breaking dormancy. The mDVR model had the most accurate prediction of full bloom during the study period compared with the other models. The DVR model usually had poor skills in prediction of full bloom dates. In particular, the error of the DVR model was large especially in southern coastal areas (e.g., Ulju and Sacheon) where the temperature was warm. Our results indicated that the mDVR model had relatively consistent accuracy in prediction of full bloom dates over region and years of interest. When observation data for full bloom date are compiled for an extended period, the full bloom date can be predicted with greater accuracy improving the mDVR model further.

Pressure Drop of Integrated Hybrid System and Microbe-population Distribution of Biofilter-media (통합 하이브리드시스템의 압력강하 거동 및 바이오필터 담체의 미생물 population 분포)

  • Lee, Eun Ju;Lim, Kwang-Hee
    • Korean Chemical Engineering Research
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    • v.60 no.1
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    • pp.116-124
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    • 2022
  • In this study, waste air containing ethanol and hydrogen sulfide, was treated by an integrated hybrid system composed of two alternatively-operating UV/photocatalytic reactor-process and biofilter processes of a biofilter system having two units with an improved design (R reactor) and a conventional biofilter (L reactor). Both a pressure drop (△p) per unit process of the integrated hybrid system and a microbe-population-distribution of each biofilter process were observed. The △p of the UV/photocatalytic reactor process turned out very negligible. The △p of the L reactor was observed to increase continuously to 4.0~5.0 mmH2O (i.e., 5.0~6.25 mmH2O/m). In case of R reactor, its △p showed the one below ca. 16~20% of the △p of the L reactor. Adopting such microbes-carrying biofilter media with high porosity as waste-tire crumb media, and the improved biofilter design, contributed to △p of this study, reduced by ca. 37~50% and 40~53%, respectively, from the reported △p of conventional biofilter packed with biofilter media of the mixture (50:50) of wood chip and wood bark. In addition, the △p of R reactor in this study, reduced by ca. 80% from the reported △p of conventional biofilter packed with biofilter media of the mixture (75:25) of scoria with high porosity and compost, was mainly attributed to adopting the improved biofilter design. On the other hand, in case of L reactor, the CFU counts in its lowest column was analyzed double as much as those in any other columns. However, in case of R reactor, its CFU counts were bigger by 50% than the one of L reactor and its microbes were evenly distributed at its higher and lower columns of Rdn reactor and Rup reactor. This phenomena was attributed to an even moisture distribution of 50~55% of R reactor at its higher and lower columns. Therefore, R reactor showed superb characteristics in terms of both △p and microbe-population-distribution, compared to L reactor.

Construction Techniques of Earthen Fortifications in the Hanseong Period of Baekje Kingdom (백제 한성기 토성의 축조기술)

  • LEE, Hyeokhee
    • Korean Journal of Heritage: History & Science
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    • v.55 no.2
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    • pp.168-184
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    • 2022
  • This paper examined the construction techniques of the earthen fortifications in the Hanseong Period of Baekje Kingdom, which has been researched most frequently among the Three Kingdoms. The construction processes of the Earthen Fortifications were reviewed and dividing into 'selection of location and construction of the base', 'construction of the wall', and 'finish, extension and repair'. The results show that various techniques were mobilized for building these earthen fortifications. Techniques which were adequate for the topography were utilized for reinforcing the base, and several other techniques were used for constructing the wall. In particular, techniques for wall construction may be clearly divided into those of the fill(盛土) and panchuk(版築) techniques. The fill method has been assumed since the 2000s to have been more efficient than the panchuk technique. This method never uses the structure of the panchuk technique and is characterized by a complex soil layer line, an alternate fill, use of 'earth mound(土堤)'/'clay clod(土塊)', and junctions of oval fill units. The fill method allows us to understand active technological sharing and application among the embankment structures in the period of the Three Kingdoms. The panchuk technique is used to construct a wall using a stamped earthen structure. This technique is divided into types B1 and B2 according to the height, scale, and extension method of the structure. Type B1 precedes B2, which was introduced in the late Hanseong Period. Staring with the Pungnap Earthen Fortification in Seoul, the panchuk technique seems to have spread throughout South Korea. The techniques of the fill and panchuk techniques coexisted at the time when they appeared, but panchuk earthen fortifications gradually dominated. Both techniques have completely different methods for the soil layers, and they have opposite orders of construction. Accordingly, it is assumed that both have different technical systems. The construction techniques of the earthen fortifications began from the Hanseong Period of Baekje Kingdom and were handed down and developed until the Woongjin-Sabi Periods. In the process, it seems that there existed active interactions with other nations. Recently, since studies of the earthen fortifications have been increasing mainly in the southern areas, it is expected that comparative analysis with neighboring countries will be done intensively.