• Mass Spectrometry Letters
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• v.7 no.1
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• pp.16-20
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• 2016

Characterization of intact protein structures in the gas phase using electrospray ionization combined with ion mobility mass spectrometry has become an important tool of research. However, the biophysical properties that govern the structures of protein ions in the gas phase remain to be understood. Here, we investigated the impact of host-guest complexation of ubiquitin (Ubq) with macrocyclic host molecules, cucurbit[n]urils (CB[n]s, n = 6, 7), on its structure in the gas phase. We found that CB[n] complexation induces the formation of compact Ubq ions. Both CB[6] and CB[7] exhibited similar effects despite differences in their binding properties in solution. In addition, CB[n] attachment prevented Ubq from unfolding by collisional activation. Based on the experimental results, we suggest that CB[n]s prevent unfolding of Ubq during transfer to the gas phase to promote the formation of compact protein ions. Furthermore, interaction with positively charged residues per se is suggested to be the most important factor for the host-guest complexation effect.

• Journal of Korean Society for Quality Management
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• v.28 no.1
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• pp.13-26
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• 2000

It is really hard for the material factors to basically improve quality of life, since it is the only partial means of the survival and activity of life. Development of Eigen Shin-Tou-Jil(Proper Body·Earth materials), therefore, must be concentrated on providing man with essential meaning of life, not with simply economic advantage. Eigen Shin-Tou-Jil(Proper Body·Earth materials) which is formed through long passage of time in the original environments that include the climate and nature features of a special region, the representative examples are like Korea Bong-sam(a kind of genseng) of Yellow Earth etc. Unfolding the Eigen Shin-Tou-Jil(Proper Body·Earth materials) by the Algorithm of Human-ware means the development for manifesting individual eigen motives and traits as subject of behavior(Gene-ware). It is because all plants, animals, inanimate objects, including Human, have evolved with their own values in the ecosystem. It was reported that a Baeksong(white pine tree), grown well up in TongEeDong, Seoul, Korea had rarely grown up during the period suppressed by Japan. By the developments of Bio-Engineering, we also found that 40% of gene base sequence of C. Elegance(a kind of worm) is identical to that of characteristic Human. In this reason, through considering common characteristics between Human and Nature, the developments of Eigen Shin-Tou-Jil(Proper Body·Earth materials) must begin with epoch for manifesting and understanding individual's Eigen motives and traits as subject of behavior(Gene-ware)

• BMB Reports
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• v.34 no.1
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• pp.21-27
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• 2001

The structural stability of brain pyrydoxine-5'-phosphate (PNP) oxidase and the catalytic properties of the monomeric species were investigated. The unfolding of brain pyridoxine-5'-phosphate (PNP) oxidase by guanidine hydrochloride (GuHCl) was monitored by means of fluorescence and circular dichroism spectroscopy Reversible dissociation of the dimeric enzyme into subunits was attained by the addition of 2 M GuHCl. The perturbation of the secondary structure under the denaturation condition resulted in the release of the cofactor FMN. Separation of the processes of refolding and reassociation of the monomeric species was achieved by the immobilization method. Dimeric PNP oxidase was immobilized by the covalent attachment to Affi-gel 15 without any significant lass of its catalytic activity. Matrix-bound monomeric species were obtained from the reversible refolding processes. The matrix bound-monomer was found to be catalytically active, possessing only a slightly decreased specific activity when compared to the refolded dimeric enzyme. In addition, limited chymotrypsin digestion of the oxidase yields two fragments of 12 and 161 kDa with a concomitant increase of catalytic activity The catalytically active fragment was isolated by ion exchange chromatography and analyzed for association of two subunits using the FPLC gel filtration analysis. The retention time indicated that the catalytic fragment of 16 kDa behaves as a compact monomer. Taken together, these results are consistent with the hypothesis that the native quaternary structure of PNP oxidase is not a prerequisite for catalytic function, but it could play a role in the regulation.

• BMB Reports
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• v.39 no.2
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• pp.167-170
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• 2006

Bovine adenosine deaminase undergoes a nondenaturational conformational change at $29^{\circ}C$ upon heating which is characterized by a large increase in heat capacity. We have determined the transition state thermodynamics of the conformational change using a novel application of differential scanning calorimetry (DSC) which employs very slow scan rates. DSC scans at the conventional, and arbitrary, scan rate of $1^{\circ}C/min$ show no evidence of the transition. Scan rates from 0.030 to $0.20^{\circ}C/min$ reveal the transition indicating it is under kinetic control. The transition temperature $T_t$ and the transition temperature interval ${\Delta}T$ increase with scan rate. A first order rate constant $k_1$ is calculated at each $T_t$ from $k_1\;=\;r_{scan}/{\Delta}T$, where $r_{scan}$ is the scan rate, and an Arrhenius plot is constructed. Standard transition state analysis reveals an activation free energy ${\Delta}G^{\neq}$ of 88.1 kJ/mole and suggests that the conformational change has an unfolding quality that appears to be on the direct path to the physiological-temperature conformer.

• Journal of rehabilitation welfare engineering & assistive technology
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• v.5 no.1
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• pp.95-101
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• 2011

In this paper, we propose the gaussian mixture model based pattern classification algorithm of forearm electromyogram. We define the motion of 1-degree of freedom as holding and unfolding hand considering a daily life for patient with prosthetic hand. For the extraction of precise features from the EMG signals, we use the difference absolute mean value(DAMV) and the mean absolute value(MAV) to consider amplitude characteristic of EMG signals. We also propose the D_DAMV and D_MAV in order to classify the amplitude characteristic of EMG signals more precisely. In this paper, we implemented a test targeting four adult male and identified the accuracy of EMG pattern classification of two motions which are holding and unfolding hand.

• Journal of The Korean Digital Architecture Interior Association
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• v.6 no.2
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• pp.9-16
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• 2006

The research want to know about form creation method's special quality that found in digital construction design process that using digital media, and they comparison analysis that who positive using digital media, Frank O. Gehry, Peter Eisenman, Greg Lynn, UN studio's work. First, In digital design process using digital media is make full use to design unfolding step and application way can change by architect's recognition difference of using digital media and each architect's design general idea unfolding process, and it means form creation process can change. Second, In digital design process a building form doesn't design adaptation to construction system, they directly design the third dimension form. So, they deduction a new form that unification and integration. Third, the architect that appear's in digital design process's part is existing, data from position of the master which it selects the coding selects the outcome goods which appears in computer operation with coordinator role of the originality and construction sense which it is visible.

• Korean Journal of Computational Design and Engineering
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• v.14 no.2
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• pp.129-136
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• 2009

Generally, curved surfaces of ship hull are deformed by flame bending (line heating), multi-press forming, and die-less forming method. The forming methods generate the required in-plane/bending strain or displacement on the flat plate to make the curved surface. Multi-press forming imposes the forced displacements on the flat plate by controlling the position of each pressing points based upon the shape difference between the unfolded flat plate and the curved object shape. The flat plate has been obtained from the unfolding system that is independent of the ship CAD. Apparently, the curved surface and the unfolded-flat surface are expressed by different coordinate systems. Therefore, one of the issues is to find a registration of the unfolded surface and the curved shape for the purpose of minimum amount of forming works by comparing the two surfaces. This paper presents an efficient algorithm to get an optimized registration of two different surfaces in the multi-press forming of ship hull plate forming. The algorithm is based upon the ICP (Iterative Closest Point) algorithm. The algorithm consists of two iterative procedures including a transformation matrix and the closest points to minimize the distance between the unfolded surface and curved surfaces. Thereby the algorithm allows the minimized forming works in ship-hull forming.

• Journal of KIISE:Computer Systems and Theory
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• v.27 no.6
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• pp.599-607
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• 2000

This paper suggests an efficient reachability analysis method of bounded petri nets. Reachability analysis is a fundamental basis for studying the dynamic properties of any discrete event systems. However, it takes at least exponential execution time and memory space to verify in general petri nets. That is, state space explosion problem may occur. In this paper, we attack problems of previous approaches - state space explosion and restrictions to applicable petri net classes - by formulating the reachability problem as set operation over structural relations among places on an unfolding.

• Bulletin of the Korean Chemical Society
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• v.31 no.12
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• pp.3521-3524
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• 2010

MTH1880 is a hypothetical protein derived from Methanobacterium thermoautotrophicum, thermophilic methanogen. The solution structure determined by NMR spectroscopy showed that it has a novel $\alpha+\beta$-fold with a highly acidic ligand binding pocket. Since MTH1880 maintains its ultra-stable structural characteristics at both high temperature and pressure, it has been considered as an excellent model for studying protein folding. To initiate the structural and folding study of MTH1880 in proving its unusual stability, we performed the site directed mutagenesis and biochemical analysis of MTH1880 mutants. Data from circular dichroism and NMR spectroscopy suggest that the point mutations perturbed the structural stability of protein even though the secondary structure is retained. This study will provide the useful information in understanding the role of participating residues during folding-unfolding process and our result will be used in designing further folding experiments for hyper-thermopile proteins like MTH1880.

• Food Science and Biotechnology
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• v.15 no.3
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• pp.418-423
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• 2006

Egg white powders (EWPs) were produced after pH adjustment (PH 6-9) of fresh egg white, followed by spray-drying, and foaming and gelling properties of EWPs were examined. EWP produced after pH adjustment to 6.5 (EWP-6.5) resulted in significantly higher foaming ability and gel hardness than control and other pH-adjusted EWP. Significant increases in surface -SH content and surface hydrophobicity of EWP-6.5 coincided with improved foaming ability and gel hardness. Significantly higher consistency index for reconstituted EWP-6.5 indicates unfolding of egg white protein was substantially increased in EWP-6.5. Decreased a-helix content in EWP-6.5 was confirmed by circular dichroism spectral analysis. These results indicate pH adjustment prior to spray-drying leads to structural changes in egg white proteins, significantly affecting major functionalities of EWP.