• Journal of agriculture & life science
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• v.46 no.2
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• pp.19-25
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• 2012

This study is an analysis of the initial growth of Adenophora triphylla var. japonica, and the growth of their above- and underground parts by means of shading treatment in order to provide a basic data that can be used in growing A. triphylla var. japonica in mountainous area. As for the rate of sprouting by shading treatment, 25% treatment and control showed the earliest sprouting. The leaf unfolding had the same order as the rate of sprouting. The analysis of the characteristics of growth of above- and underground parts showed that the growth of aboveground parts are excellent in the 25% treatment. 25% treatment were shown to be the longest in the total length of roots and root projection area. Control were discovered to be the most prominent in root surface area, root diameter, and root volume. 75% and 25% treatment in distribution by root diameter showed 72.4% and 69.5% of very fine root(>0.5mm), respectively.

• Nuclear Engineering and Technology
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• v.49 no.7
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• pp.1369-1378
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• 2017

The present paper reports the development of a computational code based on the Adaptive Group of Ink Drop Spread (AGIDS) for reconstruction of the neutron noise sources in reactor cores. AGIDS algorithm was developed as a fuzzy inference system based on the active learning method. The main idea of the active learning method is to break a multiple input-single output system into a single input-single output system. This leads to the ability to simulate a large system with high accuracy. In the present study, vibrating absorber-type neutron noise source in an International Atomic Energy Agency-two dimensional reactor core is considered in neutron noise calculation. The neutron noise distribution in the detectors was calculated using the Galerkin finite element method. Linear approximation of the shape function in each triangle element was used in the Galerkin finite element method. Both the real and imaginary parts of the calculated neutron distribution of the detectors were considered input data in the developed computational code based on AGIDS. The output of the computational code is the strength, frequency, and position (X and Y coordinates) of the neutron noise sources. The calculated fraction of variance unexplained error for output parameters including strength, frequency, and X and Y coordinates of the considered neutron noise sources were $0.002682{\sharp}/cm^3s$, 0.002682 Hz, and 0.004254 cm and 0.006140 cm, respectively.

• Bulletin of the Korean Chemical Society
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• v.27 no.5
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• pp.642-648
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• 2006

Mushroom tyrosinase (MT) structural changes in the presence of $Cu ^{2+}$ and $Ni ^{2+}$ were studied separately. Far-UV CD spectra of the incubated MT with the either of the metal ions indicated reduction of the well-ordered secondary structure of the enzyme. Increasing in the maximum fluorescence emission of anilinonaphthalene-8-sulfonic acid (ANS) was also revealing partial unfolding caused by the conformational changes in the tertiary structure of MT. Thermodynamic studies on the chemical denaturation of MT by dodecyl trimethylammonium bromide (DTAB) showed decrease in the stability of MT in the presence of $Cu ^{2+}$ or $Ni ^{2+}$ using their activation concentrations. Both activities of MT were also assessed in the presence of different concentrations of these ions, separately, with various monophenols and their corresponding diphenols. Kinetic studies revealed that cresolase activity on p-coumaric acid was boosted in the presence of either of the metal ions, but inhibited when phenol, L-tyrosine, or 4-[(4-methylphenyl)azo]-phenol was substrate. Similarly, catecholase activity on caffeic acid was enhanced in the presence of $Cu ^{2+}$ or $Ni ^{2+}$, but inhibited when catechol, L-DOPA, or 4-[(4-methylbenzo)azo]-1,2-benzenediol was substrate. Results of this study suggest that both cations make MT more fragile and less active. However, the effect of the substrate structure on the MT allosteric behavior can not be ignored.

• Journal of the Korean Institute of Landscape Architecture
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• v.22 no.2
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• pp.81-103
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• 1994

In recent years, the visual characteristics of natural open space and greenbelt surrounding the urban landscapes have been changed with sprawling of residential areas and highrised residential buildings. Since these natural areas being the background element of residential areas are topographically sloped mountains in many cities. It is easy to be seen in the distance and it is important to preserve these areas as a visual infrastructure of the urban landscape. The purposes of this study are to extract the factors of landscape impact evaluation for these areas and to clarify the physical landscape variables representing these factors, and to infer the visual-perceptional relationships between image and landscape variables. As results, conceptional three factors were extracted with semantic differential evaluation to classified 18 landscape slide, and three regression models were established with factor score of landscapes and physical variables measured in photographs. On the basis of these relationships, visual-perceptional characteristics were discussed by analyzing the data form eye-movement recording to each of landscapes. The factors of "spatial unfolding of backdropped hilly greenspace", "horizontal quence of residential buildings", and "landscape complexity" prove to be important. And it prove important variables of "skyline of mountainous ridge" and "visual edge of building structure" in regression models and eye fixation movements.

• Journal of Applied Biological Chemistry
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• v.51 no.3
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• pp.88-94
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• 2008

Three different protein extraction methods-phenol extraction, trichloroacetic acid (TCA) precipitation, and desalting/TCA precipitation-were compared to determine the optimal reproducible high resolution 2-dimensional (2-D) electrophoresis for each chungkugjang, doenjang, and kochujang samples. The soluble proteins from Chungkugjang extracted by phenol were separated with high reproducibility and resolution, and gained 1.75- to 3-fold more protein spots on 2-D gel than those from the other methods. On the contrary, the extracted proteins from doenjang and kochujang treated by desalting/TCA precipitation method showed about 1.5- to 3.3-fold more protein spots on 2-D gel. Using the established methods, the changes in the protein profiles of the fermented soy foods were monitored during the fermentation period by 2-DE. One of the major proteins in soy, $\beta$-conglycinin $\alpha$-subuint, and some proteins with unknown functions were localized on 2-D gel as the protease-resistant proteins throughout the fermentation period of doenjang. Changes in the protein profile monitored by the established methods can provide basic information on unfolding the mechanisms of the generation of biofunctional activity in the fermented soy foods.

• Journal of the Korean housing association
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• v.23 no.2
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• pp.115-124
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• 2012

The purpose of this study was to analyze the types and characteristics of space construction in temporary small-sized housing for single-person household through the understanding and interpretation of the modern flexible lifestyle. A document research method and case studies were used to analyze and classified the spatial characteristics of temporary dwelling spaces since 2000. Findings of the study were as follows: In order to conform the concept of temporary dwelling for small-sized housing units of single-person households, the types and characteristics of space construction were divided into three aspects: (1) 'Transporting' by wheel, rotation and vehicles, (2) 'Transforming' by adapting, assembling disassembling, and folding unfolding, (3) 'Wearing Carrying' by inflatable and tented type. In conclusion, this study found two types of space construction in temporary small-sized housing. The first was 'formal aspect' which was focused on the simplicity of shape, ease of deformation and lightweight of materials. And the second was 'functional aspect' which was focused on the complex space composition, the rapidity of installation and dismantling, ease of movement and transportation. This study shows that the combination of two types of the temporality will be more ideal in temporary small-sized housing planning rather than relying on just one type.

• Journal of Sensor Science and Technology
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• v.22 no.3
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• pp.223-226
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• 2013

We designed a single polyethylene bonner sphere with several thermo-luminescence dosimeters (TLD), for measurement of neutron energy spectrum. For the separation of the neutron dosage in the neutron-gamma mixed field, we used 21 ea TLD-600s and TLD-700s, respectively. Because, TLD-600 is sensitive to neutron and gamma rays, and, TLD-700 is sensitive only to gamma-rays, we could determine the each dose by neutron and gamma rays. The neutron response function of the bonner sphere with TLDs was calculated by MCNPX (ver. 2.5.0) Monte Carlo simulation in the energy range from $10^{-1}$ to 20 MeV. For the Cf-252 standard neutron source in KRISS, we could estimate the neutron energy spectrum by unfolding method using the response function.

• Molecules and Cells
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• v.27 no.6
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• pp.681-687
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• 2009

We examined the effects of synthetic signal peptides, wild-type (WT) and export-defective mutant (MT) of ribose-binding protein, on the conformational changes of signal recognition particle 54 homologue (Ffh) in Escherichia coli. Upon interaction of Ffh with WT peptide, the intrinsic Tyr fluorescence, the transition temperature of thermal unfolding, and the GTPase activity of Ffh decreased in a peptide concentration-dependent manner, while the emission intensity of 8-anilinonaphthalene-1-sulfonic acid increased. In contrast, the secondary structure of the protein was not affected. Additionally, polarization of fluorescein-labeled WT increased upon association with Ffh. These results suggest that WT peptide induces the unfolded states of Ffh. The WT-mediated conformational change of Ffh was also revealed to be important in the interaction between SecA and Ffh. However, MT had marginal effect on these conformational changes suggesting that the in vivo functionality of signal peptide is important in the interaction with Ffh and concomitant structural change of the protein.

• BMB Reports
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• v.35 no.5
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• pp.498-507
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• 2002

A gene that encodes a thermostable protease, coined thermicin, has been isolated from Thermoanaerobacter yonseiensis that is expressed and characterized in E. coli.. In order to elucidate the molecular characteristics on thermostability of the enzyme, molecular modeling and mutagenesis technology were applied. In the modeling structure, the structural core, including the active site, was well conserved; whereas, the two loop regions were unique when compared to thermitase. The mutant enzyme with the small loop deleted (D190-I196), based on modeling structural information, showed identical enzyme activity. However, when the large loop was deleted (P233-P244), a little lower $K_m$ and even a lower kcat was found. This indicates that the large loop could influence catalytic activity. However, the unfolding temperature ($T_m$), which was determined by a differential-scanning calorimetry for the mutant enzyme deleted the small loop, was $96^{\circ}C$. This is $14^{\circ}C$ lower than that for the parent thermicin. These results suggest that the small loop may play a role in maintaining the proper folding of the enzyme at high temperatures, whereas the large loop might be related to catalysis.

• BMB Reports
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• v.39 no.3
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• pp.319-328
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• 2006

SecA protein, a cytoplasmic ATPase, plays a central role in the secretion of signal peptide-containing proteins. Here, we examined effects of signal peptide and ATP on the oligomerization, conformational change, and membrane binding of SecA. The wild-type (WT) signal peptide from the ribose-binding protein inhibited ATP binding to soluble SecA and stimulated release of ATP already bound to the protein. The signal peptide enhanced the oligomerization of soluble SecA, while ATP induced dissociation of SecA oligomer. Analysis of SecA unfolding with urea or heat revealed that the WT signal peptide induces an open conformation of soluble SecA, while ATP increased the compactness of SecA. We further obtained evidences that the signal peptide-induced oligomerization and the formation of open structure enhance the membrane binding of SecA, whereas ATP inhibits the interaction of soluble SecA with membranes. On the other hand, the complex of membrane-bound SecA and signal peptide was shown to resume nucleotide-binding activity. From these results, we propose that the translocation components affect the degree of oligomerization of soluble SecA, thereby modulating the membrane binding of SecA in early translocation pathway. A possible sequential interaction of SecA with signal peptide, ATP, and cytoplasmic membrane is discussed.