• Korean Journal of Fisheries and Aquatic Sciences
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• v.10 no.3
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• pp.151-162
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• 1977

Distribution of marine algae is diverse in Korea and the resource of edible algae is abundant marking 239,037 tons of yearly production in 1976. They have been known as a protein source and used as a supplement in Korean diet. It is necessary to estimate the potentiality and properties of usable algal proteins especially as food resources and studies of extraction and separation of the proteins, therefore, are basically required for this purpose. In this study, the influence of various factors including the sample treatment, extraction time and temperature, sample us extraction solvent ratio and pH upon the extractability of the water soluble protein was determined. And the effect of precipitation treatment for isolation of the algal protein from the extracts was also tested. Nine species of algae, the major ones in consumption as food namely Porphyra suborbiculata, Undaria pinnatifida, Hizikia fusiforme, Sargassum fulvellu, Enteromorpha linza, Codium fragile, Sargassum kjellmanianum and Ulva pertusa were collected as fresh from Kijang, Yangsan Gun, in the vicinity of Busan city. The content of crude protein $(N\times6.25)$ of the algae ranged from $9.46\%\;to\;24.14\% showing the highest value in Porphyra suborbiculata and the minimum in Hizikia fusiforme. In the effort of maceration of blending methods on the extractability, immersion freezing in dry ice-methanol solution appeared most effective yielding 1.5 to 2.5 times extractability than that of the mortar grinding method. The effect of the ratio of sample vs solvent on extractability differed from species. It was enhanced at the ratio of 1:20 (w/v) in Ulva pertusa and Enteromorpha linza while the ratio was 1:30 (w/v) for Cedium fragile, Undaria pinnatifida, Hizikia fusiferme, Sargassum fulvellum and Porphyra suborbiculata and 1:40 for Sargassum kjellmanianum respectively. The effect of extraction time and temperature was revealed differently from species which might be caused by differences in the constitution of algal tissues resulting in that the extraction for 1 hour at $50^{\circ}C$ gave the maximum extractabilily in Ulva pertusa and Enteromorpha linza, 2 hours in Porphyra suborbiculata, Hikikia fusiforme, Undaria pinnatifida, Sargassum kjellmanianum and 3 hours in Codium fragile. And the extractability was higher at $50^{\circ}C$ to $60^{\circ}C$ for the most of the tested samples except Hizikia fusiforme. The optimum pH for the extraction was 9 to 12. The recovery of extractable nitrogen to the total nitrogen was $63\%$ in average with the first extracts and $8.6\%$ with the second extracts respectively. Both extracts were prepared by 2 hour extraction at $50{\pm}1^{\circ}C$ with dry ice-methanol frozen and seasand macerated materials. And these conditions assumed to be an optimum for the extraction of water soluble algal proteins since the nitrogen content after the first extraction covered $90\%$ of the total water extractable nitrogen. In the precipitation of the extracted proteins, Barnstein method and methanol treatment seemed to be more efficient than other precipitation methods.

• Food Science and Preservation
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• v.22 no.2
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• pp.251-255
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• 2015

To optimize the medium composition of Undaria pinnatifida as a pH modulator the growth of Lactobacillus plantarum industrial scale, we analyzed the pH and growth characteristics of L. plantarum in basal medium. Subsequently, the medium compositions addition of carbon, nitrogen sources and buffering agents were optimized. When 0.5% yeast extract and 2% glucose, L. plantarum grew to maximum cell density in experimental condition. However, the growth of L. plantarum rapidly pH 4.0 in basal medium. A high alkali-ash value and low cost-effective utilization n the waste part as examined. ddition of U. pinnatifida extract alleviated the serious decrease. Among them, juice of U. pinnatifida was most helpful for the growth of L. plantarum ($36.3{\pm}1.810^8CFU/mL$). These results show that U. pinnatifida be large-scale cultivation of L. plantarum. This optimized U. pinnatifida medium can be used for safe and economical production of Lactobacillus.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.42 no.2
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• pp.131-138
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• 2009

The radioprotect effects of fractinated fucoidan, derived from Undaria pinnatifida sporophylls, were examined in lung injured rats treated with partial body irradiation. The right lung of Sprague-Dawley rats, eight separately grouped by including radiation only control group (ROG) and fucoidan+radiation treated group (FRG), were treated with various fraction of Undaria-derived fucoidan every other day by intraperitoneal injection 6 days prior to irradiation and during monitoring at 24 hours, 48 hours, 2 weeks, 4 weeks and 12-weeks post-irradiation of 6 Mev/2 Gy. The rats were euthanized at completion of the treatment. Tissue samples from the injured lung, fixed in formaldehyde using lung perfusion prior to extraction, were H/E stained for histological analysis using optical microscopy. Interstitial congestion (IC), hemorrhage in alveolar sac (HM), number of macrophage (MP) and alveolar wall thickness (AWT) as a measure of early indication of fibrosis were measured. AWTs in 24 hrs post-irradiation rats increased in comparison with $2.49{\pm}0.06{\mu}m$ of control group by $3.67{\pm}0.09{\mu}m$, $3.21{\pm}0.08{\mu}m$ (p=0.013), $2.98{\pm}0.08{\mu}m$ (p=0.00) in ROG, F1-fucoidan+radiation, and F3-fucoidan+radiation group, respectively. AWT of the ROG was further increased by $4.30{\pm}0.13{\mu}m$ in the 12 weeks post-irradiation group, but AWT on average was $2.56{\pm}0.05{\mu}m$ in the FRG. MP in the 24 hrs post-irradiation group markedly increased in comparison with $2.6{\pm}0.34/0.14\;mm^2$ of the control group by $8.0{\pm}1.48/0.14\;mm^2$ in the ROG, but it was only $3.6{\pm}0.48/0.14\;mm^2$ in F3-FRG. MP in the 12 weeks post-irradiation group was $7.2{\pm}1.28/0.14\;mm^2$ in ROG, but it was $2.8{\pm}0.37/0.14\;mm^2$ or $2.4{\pm}0.4/0.14\;mm^2$ (p<0.05) in the FRG. In addition, increased IC and HM in ROG were relatively smaller in FRG of the 24 hr and 12 weeks post-irradiation rats. In conclusion, Undaria pinnatifida sporophylls-derived fucoidan fractions exhibited radioprotectant activity on injured rat lung subjected to X-ray irradiation. However, success was variable according to the fractions and also time of injection post injury.

• Journal of Environmental Science International
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• v.24 no.10
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• pp.1285-1307
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• 2015

We constructed marine forest to restore barren grounds which are expanding in the east coast of Korea using 2 methods of (1)seedlings transplantation method and (2)underwater floating ropes method. We transplanted 3 macroalgae species, Ecklonia cava, Undaria pinnatifida, and Saccharina. japonica to construct marine forest. Blade length of Undaria pinnatifida on underwater floating ropes was $56.70{\pm}8.69mm$ in April and grew $68.75{\pm}22.30mm$ in May and $70.75{\pm}14.36mm$ in July. Blade length of S. japonica was shown 97.95-143.00mm in April to June. Blade length of Ecklonia cava was $30.50{\pm}1.91mm$ in May, $41.55{\pm}1.84mm$ in August, $45.30{\pm}2.57mm$ in November, 2009 and $45.30{\pm}1.99mm$ in February, 2010. The survey on Dangsa area, Ulsan-city in January, 2009 found a total number of 15 algal species(1 brown algae, 14 red algae species) with the highest variety at 5m depth of A station and the lowest at 8m depth of A and B stations. The March survey showed a total of 24 species (1 green algae, 1 brown algae, 22 red algae species) with the highest variety of 11 at depths of 3m and 5m of B station and the lowest of 6 at 10m of B station. In May, total biomass was 3,755.4g (green algae 1.2g, brown algae 199.0g, red algae 3,555.2g). From January, 2009, we found that E. cava was dominant at the depths of 3m and 5m of A and B stations while Peyssonnelia capensis was dominant at the depth of 8m of A station. The 8m depth of B station was dominated by Acrosorium polyneurum. In May, Grateloupia lanceolata was dominant at 8m depth of A station while other depths were dominated by Phycodrys fimbriata. In June, the dominant species were G. lanceolata at the 3m depth, E. cava at the 5m and P. fimbriata at the depths of 8m and 10m of A station. Under B station, G. lanceolata was dominant at the depths of 3m and 5m while P. fimbriata was dominant at the depths of 8m and 10m.

• Korean Journal of Environmental Agriculture
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• v.24 no.4
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• pp.370-378
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• 2005

The characteristics of heavy metal biosorption on the seaweeds were investigated to develop a biological treatment technology for wastewater polluted with heavy metals. The heavy metal biosorption on seaweeds ranked in the tallowing order: U. pinnatifida$\geq$E. stolonifera$\geq$Laminaria sp.>G. amansii. The Pb was biosorbed in the range of $93{\sim}99%$, and the Cu and Cd were biosorbed in the range of $70{\sim}80%$ at the concentration of the heavy metal of $100mg/{\ell}$ respectively. The seaweed which was pretreated with $CaCl_2$ solution improved the biosorption of the heavy metals. The temperature and pH didn't affect the biosorption of heavy metals. The Langmuir isotherm reasonably fit the data of heavy metal biosorption compared to the Freundlich isotherm. The affinity of metals on the biosorption ranked in the following order: Pb>Zn>Cu>Cd. The biosorption efficiency of the heavy metals on the U. pinnatifida decreased in the multi-component rather than the single component. The heavy metals adsorbed on the U. pinnatifida were recovered using 0.3%-NTA. U. pinnatifida among the seaweed used in this work showed the best performance for the biosorption of the heavy metals.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.9 no.1
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• pp.19-24
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• 1976

To examine the storage effect of the dry cured cultured undaria pinnetifida, its components were researched according to different places and periods of production and in heat treatment of it, how the different time, temperature and salt concentration can effect on its storage was researched as follows. In Pohang and Yeosu districts the most suitable period of processing was around the end of December and in Wando district, around the end of January. When it was heat-treated separately at $90^{\circ}C\;and\;100^{\circ}C$ there of occurred the comparative low increase of organic acids and volatile acids, and the slight decrease of pigment. When it was heat-treated in sea water and satuarated NaCl solution, the obvious change was not found in all components, and in fresh water organic acids and volatile acids were conspicuously increased. When it was heat-treated according to the different heating time (long or short), there was no remarkable change in all components, but when heat-treated for 20 sec. the decrease of carotenoid was conspicuous. When heat-treated for 40 sec. separately at $90^{\circ}C\;and\;100^{\circ}C$ in sea water, better effect for storage was resulted.

• Microbiology and Biotechnology Letters
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• v.45 no.3
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• pp.236-242
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• 2017

The conversion of marine biomass to renewable energy has been considered an alternative to fossil fuels. Butanol, in particular, can be used directly as a fuel. In this experiment, the brown alga Undaria pinnatifida was selected as a biomass for biobutanol production. Hyper thermal (HT) acid hydrolysis was used as an acid hydrolysis method to produce monosaccharides. The optimal pretreatment conditions for U. pinnatifida were determined as slurry with 10% (w/v) U. pinnatifida content and 270 mM $H_2SO_4$, and heating at $160^{\circ}C$ for 7.5 min. Enzymatic saccharification was carried out with Celluclast 1.5 L, Viscozyme L, and Ultraflo Max. The optimal saccharification condition was 12 U/ml Viscozyme L. Fermentations were carried out for the production of acetone, butanol, and ethanol by Clostridium acetobutylicum KCTC 1724, Clostridium beijerinckii KCTC 1785, and Clostridium tyrobutyricum KCTC 5387. The fermentations were carried out using a pH-control. The optimal ABE fermentation condition determined using C. acetobutylicum KCTC 1724 adapted to 160 g/l mannitol. An ABE concentration of 9.05 g/l (0.99 g/l acetone, 5.62 g/l butanol, 2.44 g/l ethanol) was obtained by the consumption of 24.14 g/l monosaccharide with $Y_{ABE}$ of 0.37 in pH 5.0.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.6
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• pp.869-874
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• 1998

A feeding trial was carried out to investigate the optimum density of juvenile abalone (Haliotis discus hannai) fed the formulated diet or macroalgae ( Undaria). Abalone with initial average shell length of 8.41 mm were stocked into three replicate tanks at four different densities of 1430, 2860, 4290, and 5720 individual/$m^2$ for 15 weeks. The formulated diet produced higher body weight and shell growth, soft body weight and survival rate than Undaria in abalone. Abalone fed the formulated diet have significantly (P<0,05) higher lipid and ash, and lower moisture and protein in soft body. Higher density resulted in lower growth and survival (P<0.05). Whereas shell growth, survival rate and weight gain were not significantly (P>0.05) different within the abalone stocked at stocking densities of 1430 and 2860 individual/$m^2$ in each diet group. Body composition of abalone was not affected by increasing density. These study indicate that the number of abalone (shell length ranges of between 8 mm and 17 mm) below 2860 individual/$m^2$ were predicted to effective density in the indoor tank culture system.

• Korean Chemical Engineering Research
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• v.48 no.6
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• pp.747-751
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• 2010

The objective of this research were to produce amino acids from freezing-dried brown seaweed(Undaria pinnatifida) powder by sub- and supercritical water hydrolysis and to characterize the products. The hydrolysis was carried out in a batch type reactor consisting of 4.6 cm inside diameter and $200cm^3$ vessel and stir made of Hastelloy 276. A stir inside the reactor was continuously moving at 100 rpm. Brown seaweed powder and 100 mL of 1% acetic acid in distilled water were charged into the reactor at a ratio of 1:100(w/v). The applied conditions were $180{\sim}374^{\circ}C$, respectively for 1 hour. The total amino acid content was found to be significantly higher in brown seaweed hydrolyzed by low temperature comparing to high temperature. The amounts of low molecular weight amino acids(glycine, alanine, serine etc) were higher than that of high molecular weight amino acids. The maximum yields of amino acids were produced at low temperature($220^{\circ}C$) with acid catalyst.

• Journal of Aquaculture
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• v.6 no.2
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• pp.71-87
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• 1993

Two forms of sea mustard, Undaria Pinnatifida were cultured in order to compare the morphological characteristics and the efficiency of production. U. Pinnatifida f. distans was collected from Wando, Korea and U. Pinnatifida f. typica was transplanted from Sanriku, Japan. The latter form grew faster in length and weight than the former. Maximum growth was shown in early April for both forms, and the growth rates decreased after this. Absolute growth rates between the two forms were quite different. Average total lengths at the harvest season was 161.1cm in U. Pinnatifida f. distans and 183.5 em in U. Pinnatifida f. typica and average weights of those two forms were 1,003.4g and 1,314.6g, respectively. The weight of available parts for the salting process of the two forms were 734.8g and 968.5g, respectively. The rates of total length versus the length of the available part of the two forms were $76\%$ and $83\%$, respectively and those of the total length versus weight of the available part of them were $43.8\%$ and $52.7\%$, respectively. The results of this study suggest that the U. pinnatifida f. typica had more available part per unit weight than that of the U. pinnatifida f. distans for the efficiency of salt processing.