• Title/Summary/Keyword: Ultraviolet aging

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Consideration of UVR's Skin Damage and Study on Development of Oral Cosmetic - Focusing on Clinical Results of Calaguala (Polypodium leucotomos) - (자외선의 피부에 대한 유해성에 따른 경구용 화장품 개발에 대한 연구 - 칼라구알라(Polypodium leucotomos)의 임상연구를 중심으로 -)

  • Chung, Moon-Suk;An, Jong-Suk;Lee, Jeong-Man
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.38 no.1
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    • pp.91-101
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    • 2012
  • This study's purpose is to research the antioxidant power of Calaguala (Polypodium leucotomos, PL) as an oral supplement: Calaguala, a native of Central America, is a fern which belongs to the family polypodiaceae protecting the skin from the ultraviolet and active oxygen free radicals. The total 14 volunteers, each female 7 & male 7, were divided into 2 groups, then exposed to UV for 30 min after taking Calaguala and placebo. The total antioxidant capacity (TAC) was analyzed by taking the blood samples from anticubital vein 3 hours after UV exposure. First, Calaguala siginifcantly decreased the concentration of active oxygen free radicals 4 weeks later. The concentration of active oxygen free radical in PL group declined more than placebo group, when measured 30 min and 3 hours after UV exposure. Second, Calaguala did not significantly increase TAC 4 weeks later, but significantly decreased TAC 3 hours after exposure, when compared with placebo group. Now days, the photo allergy and photo aging caused by air pollution and ozone destruction have drastically increased, and thus the amount of UV radiation reaching the earth's surface increased. The increase of active free oxygen radicals resulted in accelerating the diseases and aging. The first superficial protecting protection by reflection & dispersion and the second body internal protection by taking oral photo antioxidant such as Calaguala should be continuously researched.

Screening System Establishment for Potential Anti-wrinkle Agents Using Human Fibroblast Elastase (엘라스타제를 이용한 주름개선 후보물질 검색 시스템의 구축)

  • Oh, Mi-Hee;Lee, Ju-Eun;Kim, Su-Yeon;Kim, So-Young;Park, Kyoung-Chan;Yun, Hye-Young;Baek, Kwang-Jin;Kwon, Nyoun-Soo;Kim, Dong-Seok
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.35 no.1
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    • pp.19-25
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    • 2009
  • It has been reported that not only collagen but also elastin contribute to inhibit skin wrinkle formation. Ultraviolet (UV) radiation induces photo-aging on human skin. Because UV radiation increases elastase activity, it is thought that increased elastase activity could be the major reason for skin elasticity reduction and wrinkle formation by UV. In the present study to standardize elastase activity measuring system, purified elastases from porcine pancrease and human neutrophil, and cell extracts of normal human primary fibroblasts, 3T3 mouse fibroblasts, and CCD-25Sk human fibroblasts were used as various enzyme sources. Furthermore, elastase activities were compared according to concentrations of enzyme and substrate and incubation time. Phosphoramidon was used as a positive control to inhibit elastase activities of normal human primary fibroblasts and CCD-25Sk fibroblasts. However, it had no influence on the activity of porcine pancreatic elastase. Therefore, it is suggested that elastase used for testing anti-wrinkle agents should be selected carefully.

The New Technology Development Strategy of Cosmeceuticals with Use Advanced Materials Resources (신소재 자원을 활용한 기능성화장품의 신기술 개발전략)

  • Kim Ju-Duck
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.30 no.3 s.47
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    • pp.427-438
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    • 2004
  • Cosmeceuticals products we not the products simply for moisturizing and protecting skin but the ones for encouraging to make improvements on wrinkles and helping to whiten, tan, and protect skin against ultraviolet light. In a broader sense, they refer to products with aiming to maintain healthy condition of skin such that skin troubles could be improved or aging of skin could be delayed. However, cosmeceuticals should not have any side effects because they are applied to the whole body for a long period of time differing from medical products which are used on specific areas for a short time. The number of such cosmeceuticals has increased from 500 in 2001 to 2300 in 2003. In order to develop and broaden the scope of cosmeceuticals, the concept of cosmetics needs to be changed from caring skin to delaying aging of the skin and reviving cells. For this purpose, high-valued materials should be developed through basic researches related to the biological function of skin, in vivo experiment and reaction of skin in response to various stimuli using biotechnologies and bioengineering methods. At the same time, it should be proceeded to develop new nano materials for overcoming skin barriers and transfer matericals for helping to absorb effective substances and maintaining stability.

Effects of Ethyl Acetate Fraction from Melothria Heterophylla on Antioxidant Activity and Matrix Metalloproteinase-1 Expression in Ultraviolet A-irradiated Human Dermal Fibroblasts (백렴 에틸 아세테이트 층의 항산화 활성과 Matrix Metalloproteinase-1 발현 저해효과)

  • Cho, Young-Ho;;Sim, Gwan-Sub;Lee, Dong-Hwan;Lee, Bum-Chun;Pyo, Heong-Bae
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.31 no.1 s.49
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    • pp.103-109
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    • 2005
  • UV irradiation leads to distinct changes in skin connective tissue, which is degradation of collagen. Many of these alterations in the extracellular matrix are mediated by matrix metalloproteinases. In this study, to develop a new anti-aging agent, we screened the antioxidant activity of solvent fractions from ethanolic extract of Melothria Heterophylla. Among the four solvent fractions tested, the EtOAc fraction exhibited the highest antioxidant activity. It was investigated the inhibitory effect of the EtOAc fraction on the expression and activity of MMP-1 in UVA-irradiated human dermal fibroblasts. The EtOAc fraction inhibited the activity of MMP-1 in a dose dependent manner with the $IC_{50}$ values of $9{\mu}g/mL$. Also, UVA-induced MMP-1 expression was reduced about $90\%$ by $100{\mu}g/mL$ of the EtOAc fraction but MMP-1 mRNA expression was not inhibited. Therefore, we conclude that the EtOAc fraction significantly inhibits MMP-1 expression at the protein level. From these results, we suggest that the EtOAc fraction from M. heterophylla could be used as a new anti-aging agent for the photo-damaged skin.

Glycation Inhibitory and Antioxidative Activities of Ergothioneine (에르고티오네인의 당화 억제 및 항산화 활성에 관한 연구)

  • Bae, Jun-Tae;Lee, Chung-Hee;Lee, Geun-Soo;Kim, Jin-Hwa;Hong, Jin-Tae
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.45 no.2
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    • pp.151-159
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    • 2019
  • Ergothioneine has been known as an excellent antioxidant and a cellular protector against oxidative damage in vivo. In the present study, ergothioneine was demonstrated to possess antioxidant and anti-glycation activities. The radical scavenging activity of ergothioneine enhanced the viability of human dermal fibroblasts (HDFs) exposed to ultraviolet (UV) light. The UVA irradiation increased the proportion of senescence-associated ${\beta}$-galactosidase (SA-${\beta}$-gal) positive cells in comparison with the normal control group. The treatment of UVA-irradiated HDFs with ergothioneine decreased the level of SA-b-gal (by approximately 45% at an ergothioneine concentration of $400{\mu}M$) compared with the UVA-irradiated HDFs. We also found that ergothioneine inhibited production of glyceraldehyde-derived advanced glycation endproducts (AGEs) in a concentration-dependent manner. The ergothioneine educed carboxymethyl-lysine (CML) expression in comparison to the glyoxal treatment. In addition, in the Western blot analysis, treatment of glyoxal-stimulated HDFs with ergothioneine resulted in a dose-dependent decrease in the expression level of the receptor for AGE (RAGE). These results suggest that ergothioneine may have potent anti-aging effects and could be used as a cosmetic material against cellular accumulation of AGEs.

Effect of pomegranate concentration solution on photoaging (석류농축액의 광노화에 의한 주름 개선 효과)

  • Kang, Su Jin;Choi, Beom Rak;Kim, Seung Hee;Yi, Hae Yeon;Park, Hye Rim;Song, Chang Hyun;Park, Soo Jin;Ku, Sae Kwang;Lee, Young Joon
    • Journal of Society of Preventive Korean Medicine
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    • v.19 no.1
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    • pp.109-116
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    • 2015
  • Objective : Solar ultraviolet (UV) light can cause photoaging of human skin. Many researchers have focused on effective prevention to mitigate the aging process. This study was aimed to investigate anti-wrinkle effects of pomegranate concentration solution (PCS) using photoaged hairless mouse model. Method : To clarify the protective effect of PCS against UVB-induced photo-damage, water capacity was measured with transepidermal water loss (TEWL) in SKH-1 hairless mice. In addition, image of skin replicas was analyzed. Results : Our results showed that the PCS treatment protects skin against UVB-induced photoaging, based on the parameters including moisture capacity and TEWL. According to visual assessment of skin replica, application of 1 ml/kg PCS inhibited UV-induced wrinkle formation in mice skin as compared to the vehicle-applied control mice. Conclusion : Taken together, these results indicated that PCS could improve skin wrinkle formation induced by UVB irradiation in SHK-1 hairless mice. PCS could be applied as an anti-wrinkle agent.

A study on the color controlled of painter's work (페인트 도장공사의 색관리에 관한 연구)

  • Shim, Myung-Sup;Lee, Hyun-Jeong
    • Journal of the Korea Institute of Building Construction
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    • v.3 no.1
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    • pp.107-114
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    • 2003
  • This study aims to find methods that prevents aging of buildings paint coating and that limits defects in construction. Defects in painting can occur in four stages: pure paint, during painting, after the paint coating has dried, and after some period of time has passed after coating. Paint may become bad due to precipitation of pigments, formation of membranes, and seeding during manufacturing. Therefore, it is important that the paint is well mixed and kept airtight at a cool, dark place. Indents, paint brush strokes, orange peel, separation of colors, and paint running and spreading during the paint work process can be prevented by using high quality materials and applying a high-level of construction method. After the paint coating has dried, boiling, yellowing, poor drying, poor bonding, and/or glen deficiency may occur. These are influenced by the levels of cleanness of the dried product, drying temperature and hydration. Then, when the coating has been left dried for some period of time, cracking, peeling, scaling, swelling, discoloring, and/or rusting may develop due to the ultraviolet and contaminants in the air. Since these defects occur due to inappropriate construction schedule and/or hot and humid condition, one must use weatherproof materials. Furthermore, poor paint color may be caused by contamination in the sample plate, discoloration, and/or discrepancies in colors which are due to material differences, level of glossiness, degree of dispersion, dual color property of metallic colors, precipitation of pigments, etc. One should achieve reduction in construction cost and effectiveness in paint work by limiting contaminations in the construction site and strictly observing to construction regulations.

Effects of Water-Repellent on the Color of Water Paint (발수제의 혼입이 수성페인트의 색에 미치는 영향)

  • Nam, Gee-Yung;Kim, Ji-Hyun;Lee, Jae-Yong
    • Journal of the Korea Institute of Building Construction
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    • v.15 no.6
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    • pp.607-614
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    • 2015
  • Generally surface finishing of building is done with water paint that performs basic water repellency and resisting. When painting the exterior of the building with water paint, application method is easy and inexpensive and it has the advantage of building maintenance. But surface finishing of water paint is easy to discolored or aging due to ultraviolet light so the effect is not satisfying than expected. Therefore this study is to analyze durability of water-repellent paints mixed with water-repellents as outer surface finishing materials, and evaluate its feasibility. Color change and color different due to the accelerated time were tested, and the feasibility was evaluated based on the test results. The experimental results of color different, brightness different and chromaticity suggested that water-repellent paints mixed with water-repellents were suitable for finishing materials. Considering overall general durability performances, stable mixing ratios were 2, 5, and 8%.

Effects of Rubus coreanus Extracts on Ultraviolet-A Irradiated Cultured Human Skin Fibroblasts (자외선이 조사된 인간피부섬유아세포에 복분자 추출물이 미치는 영향)

  • Jeong, Hyang-Suk;Ha, Ji-Hye;Kim, Young;Oh, Sung-Ho;Kim, Seoung-Seop;Jeong, Myoung-Hoon;Lee, Hyeon-Yong
    • Korean Journal of Medicinal Crop Science
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    • v.17 no.5
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    • pp.321-327
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    • 2009
  • Sunlight, and in particular its UV component, is the major environmental trigger that underlies the major signs of human skin and skin cancer in general. Therefore, this study was carried out to investigate the UV protection effects of R. coreanus. R. coreanus was extracted by ultra high pressure extraction process at 500 MPa and $30^{\circ}C$ for 5 and 15 minutes. The cytotoxicity of the extracts extracted by ultra high pressure process on human dermal fibroblast cell CCD-986sk, human kidney normal cell HEK293, and human lung normal cell HEL299 was measured as 17.5%, 16.5% and 14.0%, respectively in adding $1.0\;mg/m{\ell}$ of the samples, which was much lower than that from conventional water extraction method at $100^{\circ}C$ as 23.2%, 22.5%, 21.2%. The secretion of $NO^-$ from macrophage showed $15.9\;{\mu}M$ on the R. coreanus extract from this process, which was higher than others. Prostaglandin $E_2$ ($PGE_2$) production from UV-induced human skin cells was also greatly decreased down to $510\;pg/m{\ell}$, compared to the control. From the results, we considered that the extracts from R. coreanus could be potent natural materials for skin anti-inflammation agent, and could be used as a potential anti-aging for the photo-damaged skin.

Evaluation of Cosmeceutical Properties of Fish Skin By-product Hydrolysates Collected During Surimi Manufacturing Process (연육 제조과정에서 발생하는 어피 부산물의 활용을 위한 Cosmeceutical 활성 평가)

  • Oh, Jae-Young;Lee, Hyo-Geun;Je, Jun-Geon;Wang, Lei;Kim, Hyun-Soo;Jeon, You-Jin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.53 no.3
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    • pp.297-307
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    • 2020
  • This study investigated the cosmetic effects of enzymatic hydrolytes of an aquatic by-product, fish skin. The skins of olive flounder Paralichthys olivaceus (PO) and Alaska pollock Gadus chalcogrammus (AP) were hydrolyzed using pepsin, Alcalase, and Protemax. Three enzymatic hydrolytes were obtained and the inhibitory effects of these hydrolytes on the aging-related enzymes tyrosinase, elastase, and collagenase were determined. The results indicated that the pepsin hydrolytes of PO and PA had stronger activities than the other hydrolytes. PO and PA also significantly reduced the intracellular reactive oxygen species levels in and improved the viability of H2O2-treated Vero cells; decreased nitric oxide production by and increased the cell viability of lipopolysaccharide-treated RAW 264.7 cells; and reduced intracellular reactive oxygen species levels and improved the viability of ultraviolet B irradiated HaCaT cells and human dermal fibroblasts. Furthermore, PO and PA remarkably reduced the intra- and extracellular melanin contents of alpha-melanocyte-stimulating hormone-stimulated B16F10 cells. These results demonstrate that PO and PA have potential for use in the cosmetic industry.