• Microbiology and Biotechnology Letters
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• v.47 no.4
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• pp.555-564
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• 2019

Biodiesel is produced through the transesterification process in the presence of alcohol and a catalyst that catalyzes the conversion of triglycerides to esters and glycerol compounds. A more optimal product conversion can be achieved using enzymes, such as lipase. Lipase is reported to be produced in osmophilic yeasts due to the low water content in their natural habitats. Wild forest honey is one of the osmophilic natural habitats in Indonesia. However, lipase-producing yeast has not been reported in the Indonesian honey. In this study, we screened the lipase-producing yeasts isolated from wild forest honey collected from Central Sulawesi. The production profile and activity of lipase were determined at different pH values and temperatures. One promising yeast was isolated from the honey, which was identified as Zygosaccharomyces mellis SG 1.2 based on ITS sequence. The maximum lipase production (24.56 ± 1.30 U/mg biomass) was achieved by culturing the strain in a medium containing 2% olive oil as a carbon source at pH 7 and 30℃ for 40 h. The optimum pH and temperature for lipase activity were 6 and 55℃, respectively. The enzyme maintained 80% of its activity upon incubation at 25℃ for 4 h. However, the enzyme activity decreased by more than 50% upon incubation at 35 and 40℃ for 2 h. This is the first study to report the lipase producing capability of Z. mellis. Further studies are needed to optimize the enzyme production.

• Computers and Concrete
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• v.14 no.2
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• pp.145-161
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• 2014

The article presents the statistical method of grouping the results of the compressive strength of concrete in continuous production. It describes the method of dividing the series of compressive strength results into batches of statistically stable strength parameters at specific time intervals, based on the standardized concept of "concrete family". The article presents the examples of calculations made for two series of concrete strength results, from which sets of decreased strength parameters were separated. When assessing the quality of concrete elements and concrete road surfaces, the principal issue is the control of the compressive strength parameters of concrete. Large quantities of concrete mix manufactured in a continuous way should be subject to continuous control. Standardized approach to assessing the concrete strength proves to be insufficient because it does not allow for the detection of subsets of the decreased strength results, which in turn makes it impossible to make adjustments to the concrete manufacturing process and to identify particular product or area on site with decreased concrete strength. In this article two independent methods of grouping the test results of concrete with statistically stable strength parameters were proposed, involving verification of statistical hypothesis based on statistical tests: Student's t-test and Mann - Whitney - U test.

• 한국연소학회:학술대회논문집
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• 2000.05a
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• pp.122-132
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• 2000

Computational fluid dynamic simulation is performed for the floating turbulent premixed flames stabilized in stagnation flows of Cho et al. [1] and Cheng and Shepherd [2]. They are both in the wrinkled flamelet regime far from the extinction limit with $u'/S^{0}_{L}$ less than unity. The turbulent flux is given in the first moment closure as a sum of the classical gradient flux due to turbulent motions and the countergradient flux due to thermal expansion. The parameter $N_{B}'s$ are greater than unity with the countergradient flux dominant over the gradient flux. The countergradient flux is assumed to be zero in $\bar{c}<0.05$. The flame surface density is modeled as a symmetric parabolic function with respect to $\bar{c}$. The product of the maximum flame surface density and the mean stretch factor is considered as a tuning constant to match the flame location. Good agreement is achieved with the measured $\tilde{w}$ and $\bar{c}$ profiles along the axis in both flames.

• Microbiology and Biotechnology Letters
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• v.22 no.1
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• pp.73-79
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• 1994

The optimum cultural temperature and time for the pullulanase production by Bacillus cereus subsp. mycoides were 35$\circ $C and 48 hrs, respectively. The addition of egg albumin and casein to the basal medium increased the enzyme production. The enzyme was purified by ammonium sulfate fractionation and DEAE-cellulose column chromatography. specific activity of the purified enzyme was 82.37 U/mg protein and yield of theenzyume activity was 62.1%. The purified enzuyme showed a single band on ployacrylamide disc gel electrophoresis and its molecular weight was estimated to be 66.,000 by SDS-polyacrylamide disc gel electrophoresis. The isoelcular point for the purified enzyme was pH 5.0. The optimum temperature and pH were 50$\circ $C and pH 6.5, respectively. The purified enzyme was stable below 40$\circ $C and in the pH range of 6.5~10.0 The pullulanase activity was greatly inhited by Ag$^{+}$, Hg$^{2+}$ and EDTA, and its heat stability was increased by the addition of Ca$^{2+}$. The tydrolysis product with the enzyme on pullulan was maltotriose.

• Korean Journal of Optics and Photonics
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• v.11 no.4
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• pp.294-298
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• 2000

The output characteristics of intracavity frequency doubling of laser-diode end-pumped Nd:S-VAP laser were investigated. Nd:S-VAP is suitable for a microchip laser medium, which has a low threshold property because of a very high value of the stimulated emission cross-section and lifetime product. The threshold energy measured was 81 J.ll. The second harmonic output energy measured was $126\mu\textrm{J}$at a pump energy of $2\mu\textrm{J}$. We described for intracavity frequency doubling by using theoretical calculations. Q-switched second harmonic energy measured was $15\mu\textrm{J}$per pulse with a pulse-width of 26 ns. at a pump energy of 2 mJ and an $M^2$ of 1.47 represented a good beam quality. ality.

• Journal of the Mineralogical Society of Korea
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• v.9 no.1
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• pp.7-16
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• 1996

Partially sericitized tourmaline from a pegmatite, Black Hills, South Dakota, U.S.A., was investigated using high-resolution transmission electron microscopy (HRTEM). Muscovite occurs as the only alteration product of tourmaline, and it is developed extensively as narrow veinlets along the {110} and {100} cleavage directions of tourmaline, indicating that a cleavage-controlled alteration mechanism was dominant. Muscovite was characterized mainly as two-layer polytypes with minor stacking disorder, but tourmaline is almost free of structural defects. HRTEM images of tourmaline-muscovite interfaces revealed that the interfaces between two minerals are composed of well-defined {110} and {100} boundaries of tourmaline. The (001) of muscovite is in general parallel to the c-axis of tourmaline, but tourmaline and replacing muscovite do not show specific crystallographic orientation relationship; muscovite consists of numerous 100-1000$\AA$ thick subparallel packets, and the angles between the (001) of muscovite and (110) of tourmaline is highly variable. Al/Si ratios of both minerals suggest that tourmaline to muscovite alteration by late magnetic fluids has been facilitated by their similar Al/Si ratio in the incipient alteration stage, in that the hydration reaction with preservation of Al and Si would require only addition of K+ and H2O. Aluminous minerals other than muscovite were not characterized as the alteration products of tourmaline, indicating that tourmaline reacted directly to muscovite; the tourmaline alteration apparently occurred by the presence of residual fluids in which K+ is available and silica was not undersaturated.

• Journal of Korean Public Health Nursing
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• v.3 no.1
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• pp.67-73
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• 1989

The purpose of this study was to identify relationship between anxiety and. coping in open heart surgery patients to provide basic information for nursing intervention in stress-coping paradigm. Data were collected from Aug. 1st to Aug. 31st, 1988 through individual interview for about 30 minutes. Tools for this study were 'Spielberger's state anxiety scale and Billings & Moos' coping scale. The subjects were 29 male and 27 female patients who had open heart surgery at S.N.U.H. The data were analyzed by Mean score, Percentage, Pearson product moment correlation coefficient T-test, ANOVA test, and Cronbach's reliability test. The results were as follows. (1) The reliability of coping scale was 0.751 by Cronbach's reliability test. (2) Average coping score of those patients was 2,53 (maximum score:4) and they used equally problem focused coping and emotion focused coping. (3) There was significant difference according to religion in stress·coping(F=2,495, P<0.05) (4) The high anxieties were, the more coping were, and there was significant negative correlation in stress-coping (r=-0.2807, P<0.05)

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.575-578
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• 2000

The N-acetylneuraminate lyase(NALase) from Escherichia coli was cloned and it was compared to that from Haemophilus influenzae and Clostridium perfringens. NALase from E. coli was expressed in very high level(about 6U/mg). The ManNAc Km value of three enzymes was almost the same. Pyruvate inhibited from H. influenzae was inhibited by GlcNAc in lower level than the others. The crude extract has about 30 times more activity than the cell for the substrate and product diffusion limit problem. The pH stability of three enzymes at pH 11 was also checked for its importance in the direct synthesis of Neu5Ac from GlcNAc and pyruvate at high alkaline condition.

• Proceedings of the KIEE Conference
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• 1989.07a
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• pp.306-309
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• 1989

This paper describes the Manufacturing process and electrical properties of ac thick film electroluminescent lamps which made of the mixture of ZnS:Cu,Cl phosphor powder and polymer binding materials. The phosphor layer is sandwiched between two electrodes, one of which is transparent, and is supported by a substrate. The substrate may be glass or flexible plastic or it may be metallic. In this study we manufactured suspend layer which consists of ZnS:Cu,Cl powder suspended in a NBR. As yet our results are behind other commercial product in electrical properties and brightness. However they can be improved by selection of appropriate polymer binding materials, development of blending technology.

• Proceedings of the KIEE Conference
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• 1999.07f
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• pp.2578-2580
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• 1999

In this paper, an embedded micro processor based resistance spot welding controller is introduced which has been recently developed by Hyosung Co. Ltd. The performance of rapid and constant high current control is tested experimentally. This paper shows configurations of measuring system for high current and realtime RMS conversion techniques of sampled discrete data. A digital proportional control is adopted for this system and the result shows that this new product is working well at wide range of welding current and the performance is improved compared with some other commercially available controllers that are widely used in our industries. User friendly MMI system and a computer network system to monitor each welding processes are also presented.