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Optimal Reaction Conditions and Radical Scavenging Activities for the Bioconversion of Green Tea Using Tannase (Tannase를 이용한 녹차의 생물학적 전환의 최적 조건 마련 및 라디칼 소거능)

  • Hong, Yang-Hee;Yeon, You-Kyung;Jung, Eun-Young;Shin, Kwang-Soon;Yu, Kwang-Won;Kim, Tae-Young;Suh, Hyung-Joo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.11
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    • pp.1501-1506
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    • 2011
  • In this study, we optimized the reaction conditions for the bioconversion of green tea using tannase, and to evaluate its radical scavenging activities. Tea catechins such as (-)-epigallocatechin gallate (EGCG) or (-)-epicatechin gallate (ECG) were hydrolyzed by tannase to produce (-)-epigallocatechin (EGC) or (-)-epicatechin (EC), respectively, and a common product, gallic acid. The bioconversion of tea catechins by tannase was increased as enzyme concentration, substrate concentration and incubation time for enzyme dose. The results indicated the optimum reaction conditions for tannase were tannase 30 U/mL (enzyme concentration) on 1% green tea (substrate concentration) for 1 hr (incubation time for enzyme). Tannase enhanced the radical-scavenging properties of green tea; the 2,2-azinobis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals scavenging abilities were significantly (p<0.001) greater for the tannase-treated green tea extract compared to the untreated green tea extract. It is reported that ECG has the greatest antioxidant activity among the catechins in green tea, and the release of gallic acid is considered to be beneficial because of its significant antioxidant potency. The results of this study suggest that the tannase-treated green tea increases antioxidant activities under optimum reaction conditions.

Effect of Manufacturing Technology on Functional Fertilizer and Feed through Recycling of Fishery Resources (수산부산물을 재활용한 제조방법이 기능성 비료와 사료에 미치는 영향)

  • Ann, Seoung-Won;An, Gap-Sun;Cho, Jun-Kwon;Cho, Tae-Dong
    • Journal of Environmental Science International
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    • v.25 no.11
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    • pp.1575-1582
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    • 2016
  • In this study, to provide basic information for design of a large-scale recycling system for fishery by-products, the food nutrient components, fertilizer components, and microbial composition of fertilizers and feed which were made of fishery by-products were analyzed before and after fermentation. The results of the analysis of the edible portion of fishery by-products indicated that calories per 100 g of crustaceans were the highest followed by those of fish and brown algae in order of precedence with values as follows; Korean Krill 94 Kcal, Portunus trituberculatus 65 Kcal, Lophiomus setigerus 58 Kcal, and Undaria pinnatifida 16 Kcal. As for changes in amino acids per 100 g of fishery by-products between before and after fermentation, calories per 100 g of P. trituberculatus decreased by 74.7% from 15.7 g to 4.0 g, that of L. setigerus decreased by 61.1% from 11.9 g to 4.6 g, that of Korean Krill decreased by 53.5% from 11.6 g to 5.4 g, and that of U. pinnatifida decreased by 49.4% from 1.7 g to 0.9 g. Among amino acids, those contained in fishery by-product fertilizers (liquid fertilizer) in large amounts were shown to be Glutaminic acid, Aspartic acid, Glycine, Lysin, and Leucine. The lipid content of Korean Krill decreased by 11.9% from 3.2 g to 2.8 g, that of L. setigerus increased by 2.0 times from 1.1 g to 2.2 g, that of P. trituberculatus increased by 4.5 times from 0.4 g to 1.7 g, and that of U. pinnatifida increased by 9.4 times from 0.2 g to 1.9 g. The ash (mineral) content of P. trituberculatus decreased by 82.5% from 26.2 g to 4.6 g, that of U. pinnatifida increased by 27.6% from 3.3 g to 4.2 g, that of Korean Krill increased by 21.9% from 3.1 g to 3.8 g, and that of L. setigerus increased by 88.7% from 1.2 g to 2.2 g. The microbial composition of liquid fertilizer using recycled fishery by-products was shown to be Bacteria, Actinomycetes, Fungi, Yeast, and Lactobacillus sp.

The Influence of Market in Agricultural Spatial Organization (농업공간조직에서 시장의 영향)

  • Lee, Sang-Yool
    • Journal of the Korean association of regional geographers
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    • v.3 no.2
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    • pp.151-162
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    • 1997
  • Although modern versions of the traditional Von $Th{\ddot{u}}nen$ theory have contributed to a description of spatial organization in agriculture, they did not incorporate the market mechanism as an integral part of location theory. This deficiency has been indicated and new mathematical structure has been proposed elsewhere by the author. The closed model, which simultaneously considered a basic principle of supply and demand, exposed a computational complexity. Based on the problem, this study attempts to extend market mechanism in order to consider the influence of city (market) size in agricultural location theory. To theoretically explore the economic relationship in a location theory, this study simplifies agricultural activity as just two activities in one-dimensional spatial economy. The problem has been solved by equating total supply and demand of agricultural products, and then by determining each agricultural price from the relationship. All of the mathematical problems have been arranged in matrix form. First, the traditional model and closed model have been compared by quantitative comparative statics which provides the sensitivity test for each model. The results have shown that the traditional model shows a relatively excessive change in land use, besides the deficiency of a constant agricultural price. Second, the effects of the size of market town and its population increase were examined, using the closed model. In this case, the price of agricultural product is increased, and the land use is extended outward. This proves that locational rent is related to the expansion of land use. Third, environmental uncertainty was associated with the closed model, in order to further consider the difference of farmers attitude in strategic perspective. In this study, two extreme attitudes, which reflects the maximum average expected returns and the maximum guaranteed returns, were examined in their land use and their effects on the prices of agricultural products. It was shown that the two farmers attitudes can be interconnected with location theory. Due to the exogenous data, the differences in the area of land use and total quantities of agricultural products were not clearly shown in this study. However, it was shown that the land use pattern is very different. That is, maximum guaranteed return model reveals a mixed land use pattern around the market town. Basically, this study shows some spatial and economic implications related to Von $Th{\ddot{u}}nen$ model.

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Purification of Methioninase from Pseudomonas putida and Its Effect on the Uptake of ^11C-Methionine in Vivo. (Pseudomonas putida 유래 Methioninase의 정제 및 생체내 ^11C-Methionine 섭취에 미치는 영향)

  • 변상성;박귀근
    • Microbiology and Biotechnology Letters
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    • v.31 no.4
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    • pp.377-382
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    • 2003
  • Purification of methioninase resulted in a yield of 69%, and SDS-PAGE analysis of the purified product revealed a single band of approximately 43 kDa in molecular weight. in vitro experiments with cancer cells incubated in methionine-free media demonstrated an increase in $^{11}$ C-methionine uptake to 25.8$\pm$1.1% at 6 hr, 31.8$\pm$0.8% at 24 hr, and 62.2$\pm$0.6% at 48hr, compared to controls. Treatment of the cancer cells with purified methioninase showed no decrease in survival after a 2 hr incubation with 0.01 U/ml, but survival of RR1022 cells decreased 30% after 24 to 48 hr incubation. SKOV-3 cells showed a 5% and 14% decrease in survival with 0.1 and 1 U/ml methioninase after 24 hr. After 48hr survival decreased 15% and 24% with 0.1 and 1 U/ml methioninase. Measurements of $^{11}$ C-methionine uptake in RR1022 cells demonstrated no change at 2 hr, but a 13.7$\pm$4.7% and 40.7$\pm$2.6% increase in uptake at 24 and 48 hr, respectively. SKOV-3 cells also showed no change at 2 hr, but had a 17.7$\pm$7.2% and 38.9$\pm$4.9% increase in $^{11}$ C-methionine uptake after 24 hr and 48 hr treatment with methioninase, respectively. $^{11}$ C-methionine PET imaging revealed clear visualization of both the tumors and contralateral infectious lesions. Administration of rMET appeared to result in a slight increase in tumor:nontumor contrast on $^{11}$ C-methionine PET images. Injection of purified methioninase also produced PET images where tumor uptake was higher than that of infectious lesions.

Anti-Inflammatory and Enzyme Inhibitory Activities of Polyphenols from Peanut (Arachis hypogaea L.) Hull

  • Mihyang Kim;Yeo Ul Cho;Narae Han;Jin Young Lee;Yu-Young Lee;Moon Seok Kang;Hyun-Joo Kim
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.312-312
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    • 2022
  • Peanut hull as by-product has been discarded during peanut processing. However, peanut hull contains plenty of polyphenols that shows various physiological activities. The objectives of this study were to investigate anti-inflammatory and enzyme inhibitory activities of polyphenols from 'Sinpalkwang' peanut (Arachis hypogaea L.) hull. Compounds were isolated from methanol extracts of peanut hull by preparative-high performance liquid chromatography after identifying and quantifying polyphenols using Ultra performance liquid chromatography (UPLC) and UPLC-Quadrupole time-of-flight-mass spectrometry profiling. The structures of compounds were elucidated by one-dimensional [1H, 13C] nuclear magnetic resonance (NMR) and two-dimensional NMR (correlated spectroscopy, heteronuclear single quantum coherence and heteronuclear multiple bond correlation). Three compounds were identified as 5,7-dihydroxy-4H-chromen-4-one (peak 2), luteolin (peak 4) and eriodictyol (peak 5). Significant differences in inflammatory mediator such as nitric oxide (NO), interleukin-6 (IL-6) and interleukin-1β (IL-lβ) in lipopolysaccharide stimulated Raw 264.7 macrophages and in enzyme (xanthine oxidase [XO] and α-glucosidase [AG]) inhibitory activities were observed between three compounds (p < 0.05). Peak 5 treated Raw 264.7 macrophages showed lower content of NO (16.4 uM), IL-6 (7.0 ng/mL), and IL-1β (60.6 pg/mL) than peak 2 (NO: 28.3 uM, IL-6: 11.3 ng/mL, IL-1β: 66.9 pg/mL) and peak 4 (NO: 24.7 uM, IL-6: 9.3 ng/mL, IL-1β: 62.6 pg/mL). Peak 5 showed higher XO inhibitory activity (84.7%) and higher AG inhibitory activity (52.4%) than peak 2 (XO inhibitory activity: 45.4%, AG inhibitory activity: 21.6%) and peak 4 (XO inhibitory activity: 37.9%, AG inhibitory activity: 37.5%) at concentration of 0.5mg/mL. This study suggests that peanut hull could be a potential source of anti-inflammatory and physiological materials while creating new use of discarded peanut hull as by-products concomitantly.

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Artificial Intelligence-Based Detection of Smoke Plume and Yellow Dust from GEMS Images (인공지능 기반의 GEMS 산불연기 및 황사 탐지)

  • Yemin Jeong;Youjeong Youn;Seoyeon Kim;Jonggu Kang;Soyeon Choi;Yungyo Im;Youngmin Seo;Jeong-Ah Yu;Kyoung-Hee Sung;Sang-Min Kim;Yangwon Lee
    • Korean Journal of Remote Sensing
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    • v.39 no.5_2
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    • pp.859-873
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    • 2023
  • Wildfires cause a lot of environmental and economic damage to the Earth over time. Various experiments have examined the harmful effects of wildfires. Also, studies for detecting wildfires and pollutant emissions using satellite remote sensing have been conducted for many years. The wildfire product for the Geostationary Environmental Monitoring Spectrometer (GEMS), Korea's first environmental satellite sensor, has not been provided yet. In this study, a false-color composite for better expression of wildfire smoke was created from GEMS and used in a U-Net model for wildfire detection. Then, a classification model was constructed to distinguish yellow dust from the wildfire smoke candidate pixels. The proposed method can contribute to disaster monitoring using GEMS images.

Thin Film Growth and Evaluation Method for Conventional Co-Cr Based Perpendicular Magnetic Recording Media: Problems and New Solutions

  • Saito, Shin;Hoshi, Fumikazu;Hasegawa, Daiji;Takahashi, Migaku
    • Journal of Magnetics
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    • v.7 no.3
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    • pp.115-125
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    • 2002
  • We proposed a novel method to evaluate the magnetic properties of the initial layer and the columnar structure separately for CoCr-based perpendicular recording media. We show that the thickness of the initial layer and the intrinsic magnetocrystalline anisotropy of columnar structure can be quantitatively evaluated using the plotted product of perpendicular anisotropy to magnetic film thickness versus magnetic film thickness ($K_{u{\bot}}^{ex{p.}}$ $\times$ d$_{mag.}$ vs. d$_{mag.}$ plot). Based on the analyses, it is found that: (1) compared with CoCrPtTa media, CoCrPtB media have relatively thin initial layer, and have fine grains with homogeneous columnar structure with c-plane crystallographic orientation; (2) CoCrPtB media can be grown epitaxially on Ru or CoCr/C intermediate layer, and as the result, the magnetic properties of the media within thin thickness region of d$_{mag.}$ $\leq$ 20 nm is significantly improved; (3) the key issue of material investigation for CoCr-based perpendicular recording media will be focused on how to fabricate c-plane-oriented columnar grains well isolated with nonmagnetic substance in epitaxial-growth media, while maintaining the thermal stability of the media.

Development of Manufacturing Ontology-based Quality Prediction Framework and System : Injection Molding Process (제조 온톨로지 기반 품질예측 프레임워크 및 시스템 개발 : 사출성형공정 사례)

  • Lee, Kyoung-Hun;Kang, Yong-Shin;Lee, Yong-Han
    • IE interfaces
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    • v.25 no.1
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    • pp.40-51
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    • 2012
  • Today, many manufacturing companies realize that collaboration is crucial for their survival. Especially, in the perspective of quality, the importance of collaboration is emphasized because economic loss increases exponentially while defective parts go through the process in supply chain. However, the manufacturing companies are facing two main difficulties in implementing collaborative relationships with their suppliers. First, it is difficult for the suppliers to produce reliable products due to their obsolete facilities. The problem gets worse for second- or third-tire vendors. Second, the companies experience the lack of universally understandable set of terminology and effective methodologies for knowledge representation. Ontology is one of the best approaches to expressing and processing a domain knowledge. In this paper, we propose the manufacturing ontology-based quality prediction framework to represent and share the knowledge of industrial environment and to predict product quality in manufacturing processes. In addition, we develop the ontology-based quality prediction system based on the proposed framework. We carried out a series of experiments for an injection molding process at an automotive part supplier. The experimental results demonstrated that the proposed framework and system can be successfully applicable in manufacturing industry.

ON STEIN TRANSFORMATION IN SEMIDEFINITE LINEAR COMPLEMENTARITY PROBLEMS

  • Song, Yoon J.;Shin, Seon Ho
    • Journal of applied mathematics & informatics
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    • v.32 no.1_2
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    • pp.285-295
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    • 2014
  • In the setting of semidenite linear complementarity problems on $S^n$, we focus on the Stein Transformation $S_A(X)\;:=X-AXA^T$, and show that $S_A$ is (strictly) monotone if and only if ${\nu}_r(UAU^T{\circ}\;UAU^T)$(<)${\leq}1$, for all orthogonal matrices U where ${\circ}$ is the Hadamard product and ${\nu}_r$ is the real numerical radius. In particular, we show that if ${\rho}(A)$ < 1 and ${\nu}_r(UAU^T{\circ}\;UAU^T){\leq}1$, then SDLCP($S_A$, Q) has a unique solution for all $Q{\in}S^n$. In an attempt to characterize the GUS-property of a nonmonotone $S_A$, we give an instance of a nonnormal $2{\times}2$ matrix A such that SDLCP($S_A$, Q) has a unique solution for Q either a diagonal or a symmetric positive or negative semidenite matrix. We show that this particular $S_A$ has the $P^{\prime}_2$-property.

Generation and Transmission of Progressive Solid Models U sing Cellular Topology (셀룰러 토폴로지를 이용한 프로그레시브 솔리드 모델 생성 및 전송)

  • Lee, J.Y.;Lee, J.H.;Kim, H.;Kim, H.S.
    • Korean Journal of Computational Design and Engineering
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    • v.9 no.2
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    • pp.122-132
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    • 2004
  • Progressive mesh representation and generation have become one of the most important issues in network-based computer graphics. However, current researches are mostly focused on triangular mesh models. On the other hand, solid models are widely used in industry and are applied to advanced applications such as product design and virtual assembly. Moreover, as the demand to share and transmit these solid models over the network is emerging, the generation and the transmission of progressive solid models depending on specific engineering needs and purpose are essential. In this paper, we present a Cellular Topology-based approach to generating and transmitting progressive solid models from a feature-based solid model for internet-based design and collaboration. The proposed approach introduces a new scheme for storing and transmitting solid models over the network. The Cellular Topology (CT) approach makes it possible to effectively generate progressive solid models and to efficiently transmit the models over the network with compact model size. Thus, an arbitrary solid model SM designed by a set of design features is stored as a much coarser solid model SM/sup 0/ together with a sequence of n detail records that indicate how to incrementally refine SM/sup 0/ exactly back into the original solid model SM = SM/sup 0/.