• 한국동물위생학회지
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• 제43권3호
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• pp.129-137
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• 2020

In this study, we applied biodegradable drug delivery carries (BDDC) for food-and-mouth (FMD) vaccination. After FMD vaccination using BDDC, we estimated the percentage inhibition (PI) of antibody, decomposed patterns, and histopathologic features of BDDC. PI of antibody was higher than 50 at two weeks after injection and sustained positive PI until 10 weeks after injection. BBDC injection group showed significantly an increased pattern of blood monocyte at two and three weeks after injection. According to the Micro CT, micro-cracks were observed at two weeks after injection and the morphology of BDDC was lost at four weeks after injection. For histopathological examination, acute inflammation with neutrophil infiltration and micro-metallic residues were observed around BDDC until four weeks after injection and inflammatory responses gradually decreased at 10 weeks. Based on our experiment, BDDC is considered as an alternative way to vaccine injection for veterinary applications. Our study can be used as basic data for the drug delivery system using biodegradable metals in the future.

• Journal of Korean Neurosurgical Society
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• 제60권3호
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• pp.348-354
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• 2017

Objective : To identify and investigate differences in spinal fusion between the normal and osteopenic spine in a rat model. Methods : Female Sprague Dawley rats underwent either an ovariectomy (OVX) or sham operation and were randomized into two groups: non-OVX group and OVX group. Eight weeks after OVX, unilateral lumbar spinal fusion was performed using autologous iliac bone. Bone density (BD) was measured 2 days and 8 weeks after fusion surgery. Microcomputed tomography was used to evaluate the process of bone fusion every two weeks for 8 weeks after fusion surgery. The fusion rate, fusion process, and bone volume parameters of fusion bed were compared between the two groups. Results : BD was significantly higher in the non-OVX group than in the OVX group 2 days and 8 weeks after fusion surgery. The fusion rate in the non-OVX group was higher than that in the OVX group 8 weeks after surgery (p=0.044). The bony connection of bone fragments with transverse processes and bone formation between transverse processes in non-OVX group were significantly superior to those of OVX group from 6 weeks after fusion surgery. The compactness and bone maturation of fusion bed in non-OVX were prominent compared with the non-OVX group. Conclusion : The fusion rate in OVX group was inferior to non-OVX group at late stage after fusion surgery. Bone maturation of fusion bed in the OVX group was inferior compared with the non-OVX group. Fusion enhancement strategies at early stage may be needed to patients with osteoporosis who need spine fusion surgery.

• IMMUNE NETWORK
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• 제8권3호
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• pp.67-74
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• 2008

Background: The effects of the dietary administration of two heat-inactivated whole bacteria from the Vibrionaceae family, singly or combined, on innate immune response of the rainbow trout were studied. The two bacteria (Pdp11 and 51M6), which were obtained from the skin of rainbow trout, showed in vitro characteristics that suggested they could be considered as potential fish probiotics. Methods: The fish were fed four different diets: control (non-supplemented), or diets supplemented with heat-inactivated bacteria at $10^8$ cfu/g Pdp11, $10^8$ cfu/g 51M6 or with $0.5{\times}10^8$ cfu/g Pdp11 plus $0.5{\times}10^8$ cfu/g 51M6 for 4 weeks. Six fish were sampled at weeks 1, 2, 3 and 4, and then the main humoral (natural haemolytic complement activity and serum peroxidase content) and cellular innate immune responses (leucocyte peroxidase content, phagocytosis, respiratory burst and cytotoxicity) were evaluated. Results: The serum peroxidase content and the natural haemolytic complement activity increased with time, reaching the highest values in the third and fourth weeks of feeding, respectively. The phagocytic ability of specimens fed the mixture of the two inactivated bacteria was significantly higher than in the controls after 2 and 3 weeks of treatment. The same activity increased significantly in rainbow trout fed the Pdp11 diet for 2 weeks or the 51M6 diet for 3 weeks. Respiratory burst activity was unaffected by all the experimental diets at all times assayed. Cytotoxic activity had significantly increased after 3 weeks in fish fed the 51M6 diet. Conclusion: Our results demonstrated the usefulness of incorporating inactivated probiotic bacteria into fish diets.

• The Journal of Korean Physical Therapy
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• 제29권5호
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• pp.265-270
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• 2017

Purpose: This study examined the effects of neck stabilization exercises with vibratory stimulation on the neck disability index and thickness of the deep neck flexor. Methods: Thirty subjects (control group=15, experimental group=15) with mild neck pain were enrolled in the study. The control group underwent craniocervical flexion exercise (control group, CG) and the experimental group was given craniocervical flexion exercise with vibratory stimulus (experimental group, EG) (3 sets, 3 times per week for 6 weeks). To examine the effects of exercise, the subjects were evaluated using the neck disability index (NDI), the thickness of the deep neck flexor muscle, and muscle strength. An independent and paired t-test were used to compare the effects of the exercise between the groups. Results: The NDI score of the two groups increased significantly after 6 weeks of treatment (p<0.001) and there was a significant difference between the EG group at 3 weeks (p<0.05) and 6 weeks (p<0.01). The thickness of the deep neck flexor in the CG group increased significantly after 6 weeks of treatment in all pressure stages (p<0.001). The EG group showed a significant increase after 3 and 6 weeks of treatment in all pressure stage (p<0.001), and 22 mmHg, a significant difference between 3 and 6 weeks (p<0.05) and among 24, 28, and 30 mmHg at 6 weeks (p<0.05). The maximum muscle strength of the deep neck flexion muscles increased significantly in the two groups after 6 weeks of treatment (p<0.001) and there was significant difference between the EG group at 6 weeks (p<0.01). Conclusion: Craniocervical flexion exercise with vibratory stimulus decreases the NDI, and increases the thickness of the deep neck flexor and maximum muscle strength of the deep neck flexion muscles in patients with mild neck pain.

• 한국농촌생활과학회지
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• 제6권1호
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• pp.31-39
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• 1995

This study was performed to observe how soy protein and calcium in rats fed unsaturated fat-enriched diet affect the total lipid and cholesterol contents of blood and tissues male Sprague-Dawley rats weighing approximately 220g were fed four purified diets which contained 18% (w/w) corn oil, 1% (w/w) cholesterol, two sources of protein : casein or isolated soy protein (ISP) and two levels of dietary calcium : 0.1% or 1.0%, first for four weeks (Expt. 1) and second for eight weeks (Expt. 2). The contents of total lipid, cholesterol and triglyceride in blood, liver, heart and feces were determined. After four weeks feeding, the serum lipid and cholesterol concentration was not significantly different among the groups. After eight weeks feeding, these concentrations were significantly lower in ISP-High calcium group. The serum lipid concentration was influenced by dietary protein sources and calcium levels. These results indicated that the hypolipidemic effects of soy protein and high calcium intake were partly due to decrease in lipid absorption and these effects were not detected at 4 weeks feeding but 8 weeks feeding.

• 한국임상수의학회지
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• 제16권2호
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• pp.293-299
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• 1999

Bone scintigraphy with $^{99m}Tc-methylene$ diphosphonate(MDP) performed for 20 weeks per two weeks interval respectively after fracture in seven(male 3 heads, female 4 heads) canine radius were analysed. All of bone scans performed 2 weeks to 20 weeks after fracture showed increase in generalized tracer uptake and showed localized increase in tracer uptake at the fracture site. Bone scans and ratio performed 6 weeks after fracture showed the most outstanding increase in generalized and localized tracer uptake. New bone formation had been observed from 2 weeks and they were incorporated completely on 18 weeks, they showed most activity during 6-10 weeks after fracture. It was recognized that the bone scan with $^{99m}Tc-methylene$ was quite sensitivity but low specificity on the fracture healing in canine radius.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제23권2호
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• pp.144-154
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• 2001

Dysfunction of the inferior alveolar nerve indicated by various degree of numbness of the lower lip and chin is one of the few drawbacks of mandibular osteotomy, especially Bilateral Sagittal Split Ramus Osteotomy(BSSRO) and genioplasty. Although it has been recorded throughout the history of this techniques, it is true etiology poorly understood. In this study, 22 consecutive patients under class III malocclusiion impression and undergoing orthognathic surgery(BSSRO only 11 case, BSSRO with genioplasty 11 case) were studied using 4 neurosensory test(static light touch, directional discrimination, two-point discrimination, pin pressure nociception) with post OP 1 week, 2 weeks, 4 weeks, 8 weeks, 12 weeks, 24 weeks, On control group, 10 members without trauma and nerve damage history, nerve test was accomplished. We concluded majority of patients return of sensation during post operative 24 weeks. Althought immediate nerve deficit are 92.2%, 97.2% 88.9% these are recovered to 25%, 35.72%, 10.71% at 24 weeks. Nerve recovery rate increased prominently between post 4 weeks and 8 weeks. There is no statistically difference about neurosensory deficit among the chin area. Neurosensory deficit more severe when the BSSRO with genioplasty group than the only BSSRO group. Immediate neurosensory deficit is larger left side than right side but after 6 months, there is no significantly difference between left side and right side. Static light touch and pin pressure nociception are more sensitive method of neurosensory deficit than two point discrimination.

• Journal of Acupuncture Research
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• 제22권5호
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• pp.99-109
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• 2005

Objectives : The purpose of this study was to examine the effects of Auricular acupressure with semen sinapis in obese children over a specific period of time. Methods : The study subjects included 27 obese children who were evaluated as such using Broca's Index at an elementary school located in M city. The data was collected throughout March 2, to May 31, 2005. In order to collect data, as a pre-test, characteristics of obesity and general characteristics were examined. Experimental treatments were used on Shinmun, Kijeom, Wijeom, Bijeom and Naebunbijeom. Among the auricular spots, one pill of semen sinapis was placed and pressed for five seconds at a time with exerted force and the subjects endured pain. This procedure was conducted ten tunes every morning, noon and evening for a period of six weeks. As a post-test, the degree of obesity were examined two weeks, four weeks and six weeks after acupressure had been applied. The data was collected and analysed using the SPSS/WIN 10.0 program by number, percentage, mean standard deviation, and Repeated Measure ANOVA. Result : The first hypothesis that 'The obesity of the children who had auricular acupressure with semen sinapis will show no difference over time' (two weeks, four weeks and six weeks after acupressure had been applied) was rejected(p=.000). A statistically significant difference in the obesity of the children who had auricular acupressure with semen sinapis was shown over time. The children's obesity degree reduced from 35.85% before auricular acupressure with semen sinapis to 28.67% six weeks after acupressure. Thus after six weeks of acupressure the relative degrees of obesity were reduced by 7.18%. Conclusion : Bases on the above results, it can be determined that auricular acupressure with semen sinapis can be used as an effective method of treatment in Korea, for reducing degrees of obesity in children.

• 한국임상수의학회지
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• 제38권2호
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• pp.49-55
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• 2021

We aimed to identify the biological factors during the voluntary waiting period (VWP) that are associated with subsequent reproductive performance in dairy cows. Two hundred and one pregnant dairy cows had their body condition score (BCS) evaluated and blood samples taken 4 weeks before calving, at calving, and 1, 2, 4, 6, and 8 weeks after calving, to measure serum metabolite and progesterone concentrations. In addition, any peri- and postpartum disorders within 4 weeks of calving, and the mean milk yields 4 and 8 weeks postpartum, were recorded. The cows were allocated to two groups according to whether they had become pregnant or not by 120 days after calving: a pregnant group (n = 50) and a non-pregnant group (n = 151). Analyses of serum metabolite concentrations showed that the aspartate aminotransferase and γ-glutamyltransferase activities 2 weeks (p < 0.05) and the non-esterified fatty acid concentration 6 weeks (p < 0.01) postpartum were higher in the non-pregnant than in the pregnant group. However, the albumin concentrations between 2 and 8 weeks and the total cholesterol and magnesium concentrations between 4 and 8 weeks postpartum were lower in the non-pregnant group (p < 0.05). The BCS (p < 0.05) and percentages of cows that had resumed cyclicity (p < 0.001) by 6 and 8 weeks postpartum were higher in the pregnant than in the non-pregnant group, whereas the incidence of peri- and postpartum disorders was higher in the non-pregnant group (p < 0.05). In conclusion, high serum enzyme activities, which imply liver damage, imbalances in energy metabolites and magnesium, and a higher incidence of peri- and postpartum disorders, are associated with poorer reproductive performance, whereas high BCS and early resumption of cyclicity during the VWP are associated with superior reproductive performance in dairy cows.

• 한국환경보건학회지
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• 제23권3호
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• pp.130-135
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• 1997

A pilot study was undertaken to examine the possible health effects of electromagnetic fields (EMFs). An experimental study was performed for two weeks of preexposure, exposure, postexposure period of February-May 1997 to determine changes in melatonin levels in urine of five volunteers before or after exposure to electric mattress during their normal sleeping hours. An urine sample was collected at about 8 a.m. right after getting up from each volunteer. Average melatonin levels seemed to be 1.6 times lower during EMF exposure period for two weeks than the corresponding levels during preexposure period. Mean melatonin levels in postexposure period were lower than the corresponding levels in preexposure period. It suggested that melatonin levels were likely to take more two weeks for recovering the preexposure melatonin level. Further research is underway with regard to EMF exposure effects on melatonin levels between occupational and nonoccupational groups.