• 한국식물병리학회지
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• 제10권2호
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• pp.136-139
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• 1994

Turnip mosaic virus (TuMV) was isolated from Rorippa islandica showing mild mosaic symptom in growing field of Chinese cabbage and radish. Identification of the virus was based on host range, transmission by aphids, electron micrograph, serological reaction and hybridization detection. The virus systemically infected on Chenopodium quinoa, Nicotiana clevelandii, N. glutinosa, Brassica rapa, B. campestris subsp. pekinensis and Raphanus sativus, whereas showed local infection on C. amaranticolor, Gomphrena globosa and Tetragonia tetragonoides. The virus was transmitted by aphid (Myzus persicae). The virus particle was filamentous with 720$\times$12 nm in length, and reacted positively with an antiserum of TuMV in agar gel double duffusion test. In slot-blot hybridization using the digoxigenin(DIG)-labeled RNA probe, TuMV-RNA could be detected in sap of R. islandica infected with the virus. This is the first report of a natural infection of that virus on R. islandica.

• 한국식물병리학회지
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• 제14권3호
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• pp.223-228
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• 1998

Turnip mosaic virus)TuMV-Ca) was isolated from a Chinese cabbage showing severe mosaic and black necrotic spots symptoms in Korea. The virus was identified as a strain of TuMV by its host range test, particle morphology, serology, double stranded RNA analysis. For detection of the virus, reverse transcription and polymerase chain reaction(RT-PCR) was performed with a set of 18-mer TuMV-specific primers to amplify a 876 bp DNA fragment The virus was rapidly detected from total nucleic acids of virus infected tissues as well as native viral RNA of purified virion particles by RT-PCR. Detection limit of the viral RNA by RT-PCR was 10 fg.

• The Plant Pathology Journal
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• 제17권4호
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• pp.201-204
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• 2001

Ultrastructural observation was conducted for the cells of oriental cabbage, Brassica campestris ssp. pekinensis 'Chungawang', inoculated simultaneously with Turnip mosaic virus (TuMV-ACT2-4vq) and Ribgrass mosaic virus (RMV-Ca1dn2) which were known as major destructive viruses of oriental cabbage in Korea. In cells infected with RMV alone, the virus particles were located as bundle or scattering in cytosols and vacuoles, which were typical ultrastructures of tobamovirus. Vessels of xylem were compacted with RMV particles. The cells infected only with TuMV had the cluster of virus particles scarcely and the typical potyvirus inclusions of scrolls, pinwheels, tubes and laminated aggregates in cytosols. The TuMV particles were jammed lineally between tonoplasts. In double infection, the two unrelated viruses of TuMV-ACT2-4vq and RMV-CA1dn2 were located together in a cell, and typical properties of each virus were also observed. The potyvirus inclusions and the tobamovirus particles were mixed entirely in cytoplasm. The virus particles of RMV wre presented strikingly near and in the center of potyvirus inclusions. In vascular cells, the tobamovirus particles were located abundantly than those in single infection. The potyvirus inclusions were embedded in the cluster of RMV particles in phloem parenchyma cells and the vascular elements were degenerated severely.

• The Plant Pathology Journal
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• 제37권1호
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• pp.1-23
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• 2021

Resistance to diseases caused by turnip mosaic virus (TuMV) in crop species of the family Brassicaceae has been studied extensively, especially in members of the genus Brassica. The variation in response observed on resistant and susceptible plants inoculated with different isolates of TuMV is due to a combination of the variation in the plant resistome and the variation in the virus genome. Here, we review the breadth of this variation, both at the level of variation in TuMV sequences, with one eye towards the phylogeny and evolution of the virus, and another eye towards the nature of the various responses observed in susceptible vs. different types of resistance responses. The analyses of the viral genomes allowed comparisons of pathotyped viruses on particular indicator hosts to produce clusters of host types, while the inclusion of phylogeny data and geographic location allowed the formation of the host/geographic cluster groups, the derivation of both of which are presented here. Various studies on resistance determination in particular brassica crops sometimes led to further genetic studies, in many cases to include the mapping of genes, and in some cases to the actual identification of the genes. In addition to summarizing the results from such studies done in brassica crops, as well as in radish and Arabidopsis (the latter as a potential source of candidate genes for brassica and radish), we also summarize work done using nonconventional approaches to obtaining resistance to TuMV.

• 식물병과 농업
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• 제1권1호
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• pp.45-46
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• 1995

In recent years, there were considerably severe occurrences of TuMV(turnip mosaic virus) disease on radish and Chinese cabbage cultivated at alpine or sub-alpine regions, especially more severe on young Chinese cabbage sowed after late June. Started from 1991, those were very severe in 1992 and 1994, for the number of migrated aphids was increased enormously according to the weather condition of high temperature and low humidity then. This disease started at late June to early July, and continued to late August. It seemed that TuMV was transmitted easily and completely to the young chinese cabbages, but hardly and rarely the old. The regions over 1,000m of altitude had less possibility of disease-occurring, but there was severe occurrence on the second cropping of Chinese cabbage in a year. It is considered that more researches on control method of TuMV disease will be needed very urgently.

• 식물병연구
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• 제23권1호
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• pp.60-64
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• 2017

무(Raphanus sativus L.) 육종 계통들에 대한 순무모자이크 바이러스(Turnip mosaic virus, TuMV) 저항성을 평가하기 위하여, 20개 무 육종계통들의 잎에 순무모자이크바이러스 BR 병원성을 가지는 국내 분리 계통을 즙액 접종하였다. 순무모자이크바이러스를 접종한 무 개체들은 $22^{\circ}C{\pm}3^{\circ}C$에서 재배하였으며 4주 동안 바이러스 병징 발현을 육안으로 조사하여 저항성을 평가하였다. 순무모자이크바이러스 감염에 의해서 무 육종계통들의 다른 병징 발현에 의해 분석한 결과, 16개 무 계통은 약한 모자이크, 모틀링에서 심한 모자이크 전신 병징을 보였으며 감수성으로 판별되었다. 이러한 결과는 순무모자이크바이러스 외피단백질 유전자에 대한 특이적 역전사중합효소연쇄반응에 의하여 확인되어, 순무모자이크바이러스가 병징을 발현시킨 원인이었다. 이와는 다르게 4개 무 육종계통들에서는 모자이크 등 전신 감염 병징이 발현되지 않았으며, 동일한 무 육종 계통들의 개체들에서 8주 동안 병징이 관찰되지 않았다. 역전사중합효소연쇄반응으로 조사한 결과 4개 무 육종 계통들의 상엽들에서 순무모자이크바이러스가 검출되지 않았다. 이런 결과는 4개 무 육종계통들이 순무모자이크바이러스에 대한 강한 저항성을 가지고 있음을 예시해준다.

• 식물병연구
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• 제21권3호
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• pp.235-242
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• 2015

2014년 전국 무 포장의 바이러스병 발생양상과 분포조사를 실시하였다. 바이러스 병징을 나타내는 108개 시료 중 47개 시료는 TuMV가 진단되었으며 RaMV는 3개, CMV는 2개 시료에서 각각 진단되었다. TuMV와 RaMV, RaMV와 CMV의 복합감염은 나타나지 않았으나 TuMV와 CMV의 복합감염이 2개 시료에서 진단되었다. N. benthamiana에 대한 TuMV 병원성 테스트 결과, 병징의 세기에 따라 세 개의 분리주를 나누었으며 외피단백질의 아미노산 서열 분석을 통하여 약한 병징을 나타내는 분리주 R007, 강한 병징을 나타내는 분리주 R041, R065를 선발하여 계통수 분석에 이용하였다. TuMV 계통수분석과 BLAST 검색결과 TuMV 분리주 3개와 기 보고된 분리주들 간의 외피단백질 아미노산 서열은 약 98-99%의 상동성을 나타내었으며, RaMV의 외피단백질의 경우 99%의 상동성을 나타내었고, CMV의 외피 단백질은 국내 기 보고된 분리주(GenBank : GU327363)와 동일하였다. TuMV 계통수에서 R007과 R041, R065는 서로 다른 그룹에 속하였으며 그룹간의 차이는 바이러스의 기주 선호도와 관련이 있을 것이라 예상하며 추후 TuMV의 병원성 결정인자 연구에 도움이 될 것으로 사료된다.

• The Plant Pathology Journal
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• 제19권2호
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• pp.117-122
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• 2003

Six isolates of Turnip mosaic Potyvirus (TuMV) namely, TuMV-CA7 from oriental cabbage, TuMV-TU and TuMV-TU2 from turnip, TuMV-RA from rape, TUMV-ST from stock, and TuMV-R9 from radish, and Ribgrass mosaic Tobamovirus (RMV-FG22) from oriental cabbage were isolated. Three kinds of characteristics of the six TuMV isolates were sorted by bioassay: TuMV-CA7 and TuMV-TU isolates infected mostly oriental cabbages; TuMV-ST, TuMV-TU2, and TuMV-R9 infected radishes; and TuMV-RA infected both oriental cabbages and radishes. Mixed infections of crucifers were RMV-FG22+TuMV-CA7, RMV-FG22+TuMV-TU, RMV-FG22+TuMV-RA, RMV-FG22+TuMV-ST, RMV-FG22 +TuMV-TU2 and RMV-FG22+TuMV-R9. Crops used were 'Tambok' cultivar resistant to TuMV, 'SSD63' susceptible inbred line of oriental cabbage, pure line of leaf mustard and 'Daeburyungyeorum' cultivar of radish. New specific ultrastructures of nonagon-like ring (NLR) and spiral aggregates (SA) by mixed infection with TuMV and RMV were formed in cells of crucifer plants. The NLR was made by a TuMV surrounded loosely by nine RMV particles, and the SA was formed spirally by full mixed of two virus particles. The SA had some NLR in its center, which was observed from cross sectioned SA. Host plants with specific ultrastructures expressed synergistic symptoms. Specific ultrastructures of NLR and SA were formed in combinations of RMV-FG22 and in TuMV-CA7, TuMV-TU, or TuMV-RA that could infect oriental cabbages. How-ever, no specific ultrastructures and mixing of the two virions in the same cell were observed in combinations of RMV-FG22, and TuMV-57, TuMV-TU2, or TuMV-R9 isolates haying virulence in radishes.

• 한국식물병리학회지
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• 제13권4호
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• pp.248-254
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• 1997

순무우 모자이크 바이러스 Ca 계통(TuMV-Ca)의 외피 단백질을 대장균 NM522 strain에서 발현시켰다. 발현된 바이러스 단백질은 한천젤 이중확산법, ELISA와 Western blotting을 이용하여 확인하였다. 외피단백질 발현 벡터(pGEX-Tu)의 구축은 IPTG induction site를 지니는 pGEX-KG에 TuMV-Ca 외피단백질 유전자를 결합하였다. 최적 단백질 발현 조건은 pGEX-Tu를 지니는 대장균을 액체 배지 1 ml당 $A_{595}$=0.1/ml의 농도로 접종한 후 2시간 뒤에 IPTG를 최종 농도를 1 mM로 조절하여 induction 시키는 경우였다. 합성된 목적 단백질은 발현 벡터의 특성상 GST (Glutathion S-Transferase) 단백질과 결합된 형태로 약 59 kDa의 단백질이었다. (uMV CP 33 kDa + GST 26 kDa.)

• 한국식물병리학회지
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• 제14권6호
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• pp.625-629
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• 1998

Turnip mosaic potyviruses (TuMV) were isolated from Rorippa indica and Armoracia lapathifolia showing mosaic symptoms in field. Identification of the TuMVs were carried out by host reactions of indicator plants, electron micrograph, serological properties and reverse transcription-poly-merase chain reaction (RT-PCR). Both viruses systemically infected Chenopodium quinoa, Nicotiana clevelandii, Brassica rapa, B. campestris subsp. pekinensis, B. juncea and Raphanus sativus, and developed local infection on inoculated leaves of C. quinoa, C. amaranticola, C. album, N. tabacum cv. Xanthi nc and Gomphrena grobosa. However, the viruses did not infect on N. glutinosa, Cucumis sativus and Vigna unguiculata. The filamentous particles, about 720 nm in length, and inclusion bodies were observed from the infected leaf tissues by dipping on electron microscopy. Crude sap of leaf infected with the viruses was reacted positively with an antiserum of TuMV in agar gel double diffusion. For detection of the viruses, RT-PCR was carried out with TuMV--specfic oligonucleotide primer. The RT-PCR products, a 1,092 bp DNA fragment, were obtained from naturally infected leaves of R. indica and A. lapathifolia. In inoculation test to seven cruciferous weeds with TuMV, infection occurred in Arabis glabra, Barbarea orthoceras, Capsella bursa-pastoris, Draba nomorosa var. hebecarpa, Rorippa cantoniensis and Thlaspi arvense.