• 대한한방소아과학회지
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• 제20권2호
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• pp.177-196
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• 2006

Objective : This experimental study was designed to determine the effects of BPT on obesity in vivo and in vitro. Methods : in vitro, BPTn extracts of various concentration(50, 100, 200 ${\mu}g/m{\ell}$) were added in 3T3-L1 cell. Adipocyte differentiation was measured by Oil Red O staining and Morphological examination. The expression of $C/EBP{\alpha}$ and $PPAR{\gamma}$ receptor was measured by western blot assay and RT-PCR in vivo, Rats were orally administered BPT daily for consecutive four weeks before poloxamer-407 induced hyperlipidemic state. The rats were sacrificed 24 hrs later for poloxamer-407 treated and then serum triglyceride, total cholesterol were measured ; Rats were orally administered BPT daily for consecutive four weeks before triton WR-1339 induced hyperlipidemic state. The rats were sacrificed 40 hrs later for triton WR-1339 treated and then serum triglyceride, total cholesterol were measured ; Rats with obesity were induced by the high fat-diet for six weeks and then serum triglyceride, total cholesterol, LDL-cholesterol, triglyceride, HDL-cholesterol, hydroxy radical, superoxide dismuatse activity were measured. Results : In vitro, The 3T3-L1 cells' differentiation was significantly decreased by BPT. The expression of $C/EBP{\alpha}$ and $C/EBP{\beta}$ was decreased by BPT. In vivo, BPT significantly reduced serum triglyceride, total cholesterol contents in poloxamer-407 treated rat. BPT significantly reduced serum triglyceride contents in Triton WR-1339 treated rat. Total cholesterol also reduced but did not show a significant change. BPT significantly reduced body weight gain of rat and adipose tissue mass of rats and serum triglyceride, LDL-cholesterol contents and significantly increased HDL-cholesterol, HTR(HDL-cholesterol/Total-cholesterol) in rats with obesity induced by the high fat-diet. BPT reduced blood lipid peroxide, hydroxy radical and increased superoxide dismuatse(SOD) activity.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2005년도 춘계 학술발표대회
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• pp.5-16
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• 2005

The herb of Allium victorialis var. platyphyllum (Liliaceae) has been used as an edible wild herb and to treat heart failure and gastritis. We have already reported antihyperlipidemic anti-tumor effects of this plant. To enlarge the commercial availability of this food, it was investigated whether the extracts of A. victorialis var.platyphyllum reduce hyperlipidemia and obesity or not. The plants tested in this experiment were collected from two eco-types of IS. Ullung and Mt. Odae cultivated at Pyongchang. Extracts were prepared by extracting the fresh leaves and those dried at $36^{\circ}C$ and $90^{\circ}C$, respectively. Pretreatment with the ethanolic extracts for two weeks (p.o.) reduced serum triglyceride-, total cholesterol- and LDL-cholesterol contents in rats induced by Triton WR-1339, respectively. Furthermore, oral administration of the extracts also inhibited the hyperlipidemia induced with oral diet of 30% corn oil. In the other attempt to find to alleviate the obesity, the model rats with obesity were induced by the high fat-diet for six weeks. Post-treatment with the extracts for two weeks significantly reduced the hyperlipidemia. Retroperitoneal-, epidymal- and total abdominal fat pad weights were considerably reduced at 100 mg/kg oral administration of the extracts. Increased feces lipid contents were also found in the rat treated with the extracts. The extract of Mt. Odae eco-type showed more potent activity than that of Is. Ullung one. These results suggest that use of the fresh leaves may lead to the higher activity in treatment of hyperlipidemia and obesity than of the dried one.

• 대한한방소아과학회지
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• 제20권1호
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• pp.219-240
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• 2006

Objective : This experimental study was designed to determine the anti-obestic effects of Bangpoongtongsungcankamibang(BTSK). Method : In vitro, BTSK extracts of various concentration (50,100, 200 $200{\mu}g/ml$)were added examination. The protein and mRNA expression of $C/EBP{\alpha}$ and $PPAR{\gamma}$ receptor was measured by western blot assay and RT-PCR. In vivo, BTSK extracts of various concentration (100, $200{\mu}g/ml$) were orally administered to induced hyperlipidemic rats by poloxamer-407 for consecutive four weeks and serum triglyceride, total cholesterol were measured. This method applied to induced hyperlipidemic rats by triton WR-1339, too. Obesity induced rats by the high fat-diet for six weeks were orally administered BTSK extracts of various concentration (100, $200{\mu}g/ml$) and serum triglyceride, total cholesterol, LDL-cholesterol, triglyceride, LDL-cholesterol, triglyceride, HDL-cholesterol, hydroxy radical, superoxide dismuatse activity were measured. Results : I. In vitro 1. The 3T3-L1 cells' differentiation was significantly decreased by BTSK. 2. expression of $C/EBP{\alpha}$ and $PPAR{\gamma}$ was was significantly decreased by BTSK. II. In vitro 1. BTSK significantly reduced serum triglyceride, total cholesterol, contents in poloxamer-407 treated rat. 2. BTSK significantly reduced serum triglyceride, contents in Triton WR-1339 treated rat. But Total cholesterol did not show a significant change. 3. BTSK significantly reduced body weight gain of rat and adipose tissue mass of rats and serum triglyceride, LDL-cholesterol, contents and significantly increased HDL-cholesterol, HTR(HDL-cholesterol/Total-cholesterol) in rats with obesity induced by the high fat-diet. 4. BTSK reduced blood lipid peroxide, hydroxy radical and increased superoxied dismuatse(SOD) activity.

• 대한한의학회지
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• 제16권1호통권29호
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• pp.403-418
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• 1995

In order to study the effects of Chungsimgangwhawhan on blood pressure in normal rats and rabbits, and hyperlipidemia induced by 1% cholesterol diet, triton WR-1339, and 75% fructose in mice and rats, the amount of total cholesterol, triglyceride, phospholipid and blood pressure were measured. The results were as follows : 1. After per os medication of Chungsimgangwhawhan, blood pressure in rats were increased but were not significant. 2. Chungsimgangwha-Whan showed strong effects on decreasing blood pressure, depending on the concentration in blood vessels of rabbits. 3. In the model of hyperlipidemia induced by 1% cholesterol diet in mice, Chungsimgangwhawhan showed decreasing effects on total cholesterol level in serum significantly (p<0.05) depending on the concentration, and on triglyceride (p<0.01) and phospholipid level (p<0.001) in serum. 4. In the model of hyperlipidemia induced by triton WR-1339 in rats, Chungsimgangwhawhan showed mild decreasing effects on total cholesterol level in serum, but was not significant. 5. In the model of hyperlipidemia induced by 75% fructose in rats, Chungsimgangwhawhan showed decreasing effects on total cholesterol and triglyceride level in serum significantly(p<0.01) depending on the concentration. According to the above results, Chungsimgangwhawhan showed significant decreasing effects on blood pressure and hyperlipidemia, and it is considered that it is appropriate to apply for hypertension and hyperlipidemia.

• 대한한의학회지
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• 제29권5호
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• pp.52-66
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• 2008

Objectives :Ginkgo-Chunghyul-dan (GCHD) is newly developed herbal medicine to prevent and treat stroke. In this study, we investigated whether the GCHD had antioxidant activity and anti-platelet aggregation effect in vitro and hypolipidemic activities in vivo. Methods :Anti-oxidant activity of GCHD was measured using the Blois method, anti-platelet effect of GCHD was assessed by the Born method, and hypolipidemic activities of GCHD were evaluated in corn oil- or Triton WR-1339-induced and cholesterol-fed rats. Results :GCHD showed anti-oxidant activity in the study inhibiting the formation of 1-diphenyl-2-picrylhydrazyl radicals and xanthine oxidase activity. GCHD had anti-platelet aggregation activity. GCHD significantly lowered total cholesterol (TC), triglyceride (TG), and low density lipoprotein cholesterol (LDL-C) in high cholesterol diet and Triton WR-1339 induced model TG in corn oil-induced model. GCHD had no acute toxicity at a single dosage. Conclusion : These results suggest that GCHD has the potential to treat hyperlipidemia and stroke.

• 대한한방내과학회지
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• 제18권2호
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• pp.246-267
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• 1997

In order to study the effects of Boyanghwanoh-tang(補陽還五湯) on blood pressure and hyperlipidemia, experimental study was performed on Hypertension in normal rats, SHR, and on Hyperlipidemia induced by Triton WR-1339 and 2% cholesterol Fed-diet in normal rats. Also the level of Total cholesterol, Triglyceride, LDL-cholesterol, HDL-cholesterol, Phospholipid were measured. The results are summarized as follows : 1. After Boyanghwanoh-tang(補陽還五湯) was given to normal rats and SHR, the results showed that inhibitory effects on blood pressure were not significant but exitory effects on heart beat were significant. 2. In the model of hyperlipidemia Rats induced by 2% Cholesterol Fed-Diet, Boyanghwanoh-tang(補陽還五湯) had significantly decreasing effects on Total cholesterol, Triglyceride, LDL-cholesterol, Phospholipid level in serum but no significantly increasing effects on HDL-cholesterol level in serum. 3. In the model of hyperlipidemia Rats induced by Triton WR-1339, Boyanghwanoh-tang(補陽還五湯) had significantly decreasing effects on LDL-cholesterol, Phospholipid level in serum and significantly increasing effects on HDL-cholesterol level in serum but there were not significantly decreasing effects on Total cholesterol, Triglyceride. These results show that Boyanghwanoh-tang(補陽還五湯) has significantly inhibitory effects on hyperlipidemia and thal it could be clinically applied for hyperlipidemia.

• 대한예방한의학회지
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• 제7권2호
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• pp.107-119
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• 2003

In order to study the preventive effects of hyperlipidemia depending on endogenous and exogenous methods of induction, after observing what happens when Nokyongdaebotang, a strengthening up treatment is orally administrated into the ways that cause hyperlipidemia either by the exogenous hyperlipidemia condition model method, which is the way where you orally administrate the cholesterol that was dissolved in olive oil, or the endogenous hyperlipidemia model method, where it uses the injecting the Triton WR-1339 vein method, or to the already inducted white rats, the following conclusions could be drawn. 1. The endogenous induction method, cholesterol diet, helps preventing Total Cholesterol, TG, and LDL-Cholesterol, Free Fatty Acid, Phospholipid's augmentation within the blood in the white rats that is being induced or just inducted with hyperlipidemia 2. The exogenous induction method, Triton WR injection, helps preventing Total Cholesterol, TG, and LDL-Cholesterol, Free Fatty Acid, Phospholipid's augmentation within the blood in the white rats that is being induced or just inducted with hyperlipidemia 3. The HDL-Cholesterol did not increase in regard. This is considered to be because when the geological features the HDL-Cholesterol increases proportionally. In deference to the above results, Nokyongdaebotang, which strengthens the vitality, showed that it helps prevent white rats that is being induced or just inducted with hyperlipidemia no matter whether it is endogenous or exogenous.

• Food Quality and Culture
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• 제3권1호
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• pp.40-44
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• 2009

To evaluate the hypolipidemic effects of Cheonggukjang (CGJ), which is frequently used in Korea similar to Natto in Japan and Douchi in China like a dairy product, boiled soybeans were fermented with two Bacillus strains, B. subtilis and B. licheniforms, isolated from rice straw and their antihyperlipidemic effects of their products were investigated. Treatment with the CGJs significantly reduced blood triglyceride (TG) and total cholesterol (TC) levels and increased HDL cholesterol levels in Triton WR-1339-induced hyperlipidemic mice. The treatment of non-fermented soybeans alone also reduced blood TG and TC levels, but not significantly. Feeding the CGJs significantly lowered high blood TG and TC levels as well as body and epididymal mass weights in hyperlipidemic mice induced by the long-term feeding of a high-fat diet that increased blood HDL cholesterol levels. The B. subtilis-fermented CGJ products more potently reduced TG and TC levels, although the differences between the starters were not significant. These finding suggest that CGJ products may be effective as hypolipidemic foods by the synergistic interaction of soy and Bacillus strains.

• 생약학회지
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• 제36권2호통권141호
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• pp.109-115
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• 2005

The herb of Allium victorialis var. platyphyllum (Liliaceae) has been used as an edible wild herb and to treat heart failure and gastritis. We have already reported antihyperlipidemic anti-tumor effects of this plant. To enlarge the commercial availability of this food, it was investigated whether the extracts of A. victorialis var. platyphyllum reduce hyperlipidemia and obesity or not. The plants tested in this experiment were collected from two eco-types of Is. Ullung and Mt. Odae cultivated at Pyongchang. Extracts were prepared by extracting the fresh leaves and those dried at $36^{\circ}C$ and $90^{\circ}C$, respectively. Pretreatment with the ethanolic extracts for two weeks(p.o.) reduced serum triglyceride-, total cholesterol- and LDL-cholesterol contents in rats induced by Triton WR-1339, respectively. Furthermore, oral administration of the extracts also inhibited the hyperlipidemia induced with oral diet of 30% corn oil. In the other attempt to find to alleviate the obesity, the model rats with obesity were induced by the high fat-diet for six weeks. Post-treatment with the extracts for two weeks significantly reduced the hyperlipidemia. Retroperitoneal-, epidymal- and total abdominal fat pad weights were considerably reduced at 100 mg/kg oral administration of the extracts. Increased feces lipid contents were also found in the rat treated with the extracts. The extract may lead to the higher activity in treatment of hyperlipidemia and obesity than of the dried one.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.334.2-334.2
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• 2002

As the part of our continuing study for antihyperlipidemic agents from the herbal medicinal resources. we examined the possibility of the ethylacetate fraction of Alpinia officinarum water extract in vitro and in vivo. We isolated some compounds from the ethylacetate fraction of Alpinia officinarum and measured their antihyperlipidemic activities. The active components isolated by silicagel column lmproved serum TG. HDL and LDL level in corn oil feeding and triton WR-1339 induced hyperlipidemic mice. The most active compound was 3-Methylethergalangin. Consequently. these biologically active gerbal functional foods could be used for preparing the healthy food which might diabetas. hyperlipidemia and other disease.