• 한국작물학회지
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• 제29권3호
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• pp.298-305
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• 1984

오차드그라스와 라디노클로버의 경합관계를 밝히기 위하여 기존초지에서 분주하여 동일한 밀도로 단·혼파조합을 조성하고 경합구로서 지상 및 지하를 투명아크릴판으로 격리처리하고 질소 및 인산비료를 시용하여 시비에 따른 반응을 조사하였다. 년4회 예취를 통한 목초수량에서 라디노클로버는 오차드그라스에 비하여 현저히 낮았고 LAI, 초고, 분얼력에서도 약세였다. 특히 혼파시에는 라디노클로버가 하부에 위치하며 수평엽의 초형을 가져 광리용 이 불량하였고 경합이 진행됨에 따라 점차적인 수량감소가 있었다. 이들의 경합상태를 지상부 경합과 지하부 경합으로 나누어 보았을 때 지하교 경합에서 라디노의 상대적 열세가 더욱 심하였고 이는 오차드그라스의 왕성한 근군확장과 양수분리용에서 유리하였기 때문이었다. 질소의 시비는 오차드의 경합력을 더욱 촉친하였고 인산의 시용은 라디노의 생육에 유리하게 작용하였으나 오차드에 미치지 못하였다. 혼파구의 수량은 오차드그라스의 단파수량보다 적었으나 두 초종의 평균인 'Expected Yield' 보다는 높아 혼파는 유리하였고 경합의 형태는 오차드그라스에 의한 '과잉보상'으로 오차드그라스는 라디노에 비하여 23.5％ 가량 우점도를 나타내었다.

• 한국초지조사료학회지
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• 제14권3호
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• pp.177-185
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• 1994

Slow seedling growth rate and nodulation failure of white clover (Trifolium repens L.) has been limited its good establishment to pastures. The experiment was done to determine the effect of removal of cotyledon and unifoliolate on the shoot, root growth, and nodule formation of 4 white clover cultivars for 8 weeks after the treatment. Four white clover cv. Regal (large leaf), Louisiana S-I (medium-large leaf), Grasslands Huia (mediumsmall leaf), and Aberystwyth S184 (small leaf), were grown in IOcm plastic pot containing 2:l:l soi1:sand:peat moss mixture until grown to cotyledon or unifoliolate stage and then removed one (Cl) or two cotyledons (C2) at cotyledon stage, and unifoliolate only (U), unifoliolate and one cotyledon (UCl) or unifoliolate and two cotyledons (UC2) at the unifoliolate stage, and the plants were sampled at 4, 6 and 8 weeks after the treatments. The intact plants had greater shoot and root dry weights, and no. of nodules than removal-treated ones. Removal treatments at cotyledon stage, the dry weight and no. of nodules more decreased in C1 and C2 than that of unifoliolate stage. While the severer cotyledon removal, the more reduction. Although the dry weights and no. of nodules steadily inclined with regrowing period, the former were higher in Regal and La. S-1 than in the others since 6 weeks after removal treatment but the latter was more in S 184 than in the others 8 weeks after removal treatment. Relationship between no. of nodules and shoot or root dry weight was analysed as linear mode while the earlier and severe removal, the steeper slope. It was concluded that severer damage of cotyledon and unifoliolate had detrimental effects on the shoot and root growth, nodule formation, and aftermath establishment of white clover.

• 한국환경복원기술학회지
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• 제4권1호
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• pp.1-15
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• 2001

The purpose of this study is to evaluate the creation techniques of eco-pond, one of biotopes to promote biodiversity in urban residence area. Investigation were classified out plant, mammals, amphibia, reptiles, birds, fishes and insects. The results were summarized as follows: Around the eco-pond shows simple vegetation structurs, consisted of Pinus densoflora S et Z. and Robinia pseudoacacia under competition. In case of shrub, consisted of 4 species but plant growing appearence diversely by seasons. The evaluation of vegetation of eco-pond, there are found 4 species of aquatic plants. Inside the revetment of pond, Echinochloa crus-galli, Persicaria hydropiper, Digiaria sanguinalis, Cyperus microiria and Bidens frondosa L. are mainly distributed. Near the revetment, Trifolium repens L. and Digiaria sanguinalis are prevailed. And in its background, Erigeron canadensis, Erigeron annuus and vines are begins to make their appearances. When evaluation animals in eco-pond and contrast plot, it show simple species and numbers of mammals. It seemed to be resulted from its isolation and outside intervention by users In eco-pond, Pica pica and Streptopelia orientalis are mainly found and in contrast plot of Columba livia, which are so strong adaptation to city life environment. In case of amphibia and reptiles, none is observed in contrast plot, but in ecological pond, Rana nigromaculata and Hyla japonica are constantly observed. In case of insects, more species are found in eco-pond than contrast plot. And in eco-pond, more dragonflies are visibly increased one year after its construction. In floral zone inside of pond revetment, grasshopper and Locusta migratoria are frequently observed. In case of butterflies, they are mainly found in log fence and willow(salix) around eco-pond. In case of fishes inside of eco-pond, the species and its density are remarkable increased one year after the construction. With above evaluation results, we have identify the increase effect of biodiversity after construction of the eco-pond.

• 한국유기농업학회지
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• 제20권4호
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• pp.589-605
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• 2012

This study was conducted to identify allelopathic effect of Ganghwa domestic Artemisia spp., named Sajabalssuk and Ssajuarissuk, for various receptor plants including clover (Trifolium repens L.), alfalfa (Medicago sativa L.), lawn grass (Zoysia japonica Steud.), dandelion (Taraxacum platycarpum Dahlst.), and dahurianpatrinia (Patrinia scabiosaefolia Fisch. ex Trevir). Receptor plants were treated with the aqueous and essential oil extract of Artemisia plants. In consequence, their allelopathic effects were evaluated by measuring seed germination rates, seedling growth, and dry weights of the receptor plants. The seed germination and seedling growth of the receptor plants were inhibited by all treatments of both aqueous and essential oil extracts of the Artemisia plants, and, in addition, the inhibitory effects were increased according to the higher concentration. Among the donor plants, A. $sp.^*III$ showed most effective allelopathic effect. Comparing the alleopathic effect among the receptor plants, seed germination was most inhibited in lawn grass while inhibitory effect of seedling growth was comparatively higher in dandelion. Although inhibitory effects were comparatively lower, the allelopathic effects of Artemisia plants were identified in clover and alfalfa since the seedling growth of these plants were inhibited more than 70%. Thus, in result, Ganghwa domestic Artemisia spp. could be possibly used for weed control since natural products of the plants showed inhibitory effects on seed germination and seedling growth of various receptor plants.

• 한국초지조사료학회지
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• 제6권1호
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• pp.1-5
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• 1986

본 시험은 경운(耕耘)이 불가능한 산지(山地)에 있어서 제초제(除草劑)에 의한 초지개량시험(草地改良試驗)의 일환으로서 ha당 2.9kga,i의 Glyphosate를 철포(撤布)한 후(後)에 선점관목(先占灌木)을 그대로 방치한 것과 또 살포후 관목을 10, 20, 30, 40일 간격으로 예취제거(刈取除去)하였을 때 이러한 처리가 겉뿌림한 오차드그라스 및 라디노클로버 유식물(幼植物)의 정착(定着)과 생육(生育)에 어떠한 영향을 미치는가를 구명(究明)하기 위하여 $1983{\sim}1984$년에 실시되었다. Glyphosate살포후 기존관목에 대한 다른 예취간격은 겉뿌림 목초(牧草)의 정착(定着), 유식물(幼植物)의 활력(活力), 건물수량(乾物收量) 및 개량초지(改良草地) 식생비율(植生比率)는 하둥의 상이한 영향을 주지 못하였다. 본(本) 시험결과(試驗結果)에 의하면 목초(牧草)의 겉뿌림을 위한 기존(旣存) 관목(灌木)의 제거(除去)는 Glyphosate살포후 $10{\sim}40$일 사이에 하는 것이 적절한 것으로 나타났다.

• 한국잡초학회지
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• 제30권4호
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• pp.429-436
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• 2010

온실조건에서 제초활성물질로서의 가능성을 확인한 천연물 chrysophanic acid의 간이 포장조건에서의 잡초 방제력과 살초특성을 평가하여 실용화 가능성을 검토하고자 본 연구를 수행하였다. Chrysophanic acid $2,000{\mu}g\;mL^{-1}$ 처리에서 토끼풀 방제효과는 생육시기에 관계없이 매우 우수하였으나, 약제처리 2주후에는 재생되었다. Chrysophanic acid의 쑥에 대한 방제력은 다소 미흡하였으며, 마디풀은 $2,000{\mu}g\;mL^{-1}$ 이상의 농도에서 효과적으로 방제되었으나 동시에 잔디에 대한 약해도 발생하였다. 자연발생된 다양한 잡초에 대한 방제력이 매우 우수하였는데 특히 강피, 강아지풀, 바랭이 등에 대해서는 $6,000{\mu}g\;mL^{-1}$ 처리에서 100% 살초력을 보였고, $4,000{\mu}g\;mL^{-1}$ 처리에서도 80% 이상의 방제효과가 나타났다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.357-357
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• 2017

The combined application of oil cake and rice bran into the soil surface was found useful for weed control in our previous pot study. The present study was undertaken to evaluate the performance of white clover (Trifolium repens L.) while incorporated in the paddy field and effects of combined fertilizer on weed control and rice yield. A plot was divided into two parts i.e. white clover incorporated and not incorporated. The nitrogen content of the incorporated white clover was $12.5gm^{-2}$. Chemical fertilizer and combined fertilizer plots were compared with non-fertilizer conditions. The mixed ratio of combined fertilizer was oil cake 1.35 and rice bran 1.0. Combined fertilizer was applied to the soil surface, and chemical fertilizer was mixed in the soil. Nitrogen application rate was $8gm^{-2}$ for any fertilizer. The weed numbers were significantly reduced in the white clover plot irrespective of application condition both at heading and harvest time. Also, weed control ability was improved by the use of combined fertilizer. In the not incorporated plot, the number of weeds was suppressed about 90% by applying combined fertilizer. The rice yield was markedly increased by the incorporation with white clover under all fertilization conditions. Contribution rates of increased rice yield by white clover and combined fertilizer were about 55% and about 25%, respectively. The rice yield was increased by the incorporation with white clover, and the number of weeds remarkably decreased as well. Also, these effects were improved due to combined application of oil cake and rice bran.

• 한국작물학회지
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• 제47권2호
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• pp.95-101
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• 2002

To investigate the changes in the composition and pool size of nitrogen metabolites under drought stress, white clover (Trifolium repens L.) were exposed to -0.04MPa (well-watered, control) or to -0.12MPa (drought-stressed) of soil water potential during 28 days. Dry weight of leaves in drought-stressed plants was remarkably decreased by 45% and 74% within 14 days and 28 days, respectively, compared with control. For nitrate concentration after 28 days of treatment, a significant difference (1.6 times higher in drought-stressed plants) was observed only in stolon. NH$_3$-NH$_4$$^{+}$ concentration in all three organs of drought-stressed plants linearly increased to more than 1.6 times higher level at 28 day when compared to the initial level (day 0), while the increasing rate in control was much less than that of drought-stressed plants. Proline concentrations in drought-stressed plants remarkably increased and reached to 7, 13 and 17 times higher level at 28 day compared to control. Protein concentration in leaves of drought-stressed plants tended to decrease, while it slightly increased during the first 14 days and reached a plateau afterward in control. There was not significant difference in the proteins concentration of stolon and roots throughout experimental period. On SDS-PAGE analysis, two major proteins specifically induced by drought stress (16-kD and 18-kD) were detected in stolon.n.

• Plant Biotechnology Reports
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• 제3권3호
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• pp.191-197
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• 2009

Four-leaf white clover is not found easily in nature due to its low appearance rate (1 in 10,000). Because people believe that it brings good luck and like to either keep it or present it to a loved one, it has commercial and ornamental value. To breed four-leaf clover, we exposed its flowers to ${\gamma}-rays$ at the pollination stage. The $M_1$ seeds produced following doses at 25-100 Gy showed an approximately 74% germination rate, with seedling survival at 46%. In the $M_1$ generation of plants irradiated within that dose range, we found an increased frequency of four leaflets. One of them, Jeju Lucky-1 (JL-1), had a frequency of about 60%. To see whether that mutation was somaclonal or genetic, we observed its $M_2$ generation and found that such a phenotype reappeared. Although our results demonstrated that the irradiation of fully mature flowers led to a higher frequency of 4-leaflets, we could not clearly explain the genetic mechanism involved. We suggest that JL-1 is valuable as a new variety, without further genetic fixation, because white clover can be propagated vegetatively by stolons.

• 한국초지조사료학회지
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• 제15권2호
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• pp.80-86
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• 1995

High salinity common in reclaimed soil can reduce germination of crop seeds and aftermath its establishment and production. The experiment was done to measure germination and radicle elongation of berseem, subterranean, white clover and Italian ryegrass as Control under different temperature and NaCl concentrations. Onehundred seeds of berseem (Trifolium ulexandrinum cv. Bigbee). suberranean (T. suhterruneum, cv. Nungarin), white clover (T. repens, cv. Regal) and Italian ryegrass (Lolium mulriflorum, cv. Terafloum) were placed in petri dishes with a sheet of filter paper replaced every two days, and then exposed to 0, 12.5, 25. 50, 100 and 200mh4 of sodium chloride in darkened growth chambers controlled with 10, 15 and $20^{\circ}C$. Percent germination and radicle elongation were measured. Beneem clover showed greatest daily percent germination among the cloven. That of subterranean clover, moreover, was higher than that of white clover till 4 days after imbibition but the reverse result was true thereafter. Although germination of Italian ryegrass was delayed with decreased at 200mM of NaCl of $20^{\circ}C$. at 200mM of under$15^{\circ}C$ , and at higher than lOOmh4 of all level of temperature, respectively. Berseem clover had greatest radicle length under the same concentration of NaCl regardless of temperature treatment while radicle elongation of subterranean and white clover was repressed over 50-100mM of NaC1. The slope of linear regression equation between concentration of NaCl and percent germination declined under all temperature treatment in order of berseem, subterranean and white clover whereas the reverse result was showed in radicle length.