• The Korean Journal of Pharmacology
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• v.9 no.1
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• pp.59-63
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• 1973

Although numerous drugs are available for the treatment of superficial fungi infections of skin, and yet the clinical effects of most of such drugs are not satisfactory. In the hope of searching for effective drugs for superficial fungi infections, the authors observed fungistatic effects of Pyrethri Flos, a common herb in Korea, with water extract (PFWE), ethanol extract (PFEE), and methanol extract (PFME) from Pyrethrum cinerariaefolium V. In in vitro studies, the spores of fungi were inoculated on Sabouraud's glucose agar media which contained Pyrethri Flos extracts in each concentrations of $500\;{\mu}g/ml$, $1,000\;{\mu}g/ml$ and $5,000\;{\mu}g/ml$ respectively, and the growth of the fungi was observed for 3 weeks. The species of the fungi used in these experiments were Epidermophyton floccosum, Microsporum canis, Microsporum nanum, Microsporum gypseum, Microsporum cookei, Trichophyton rubrum, Trichophyton mentagrophytes, Trichophyton tonsurans and Trichophyton verrucosum. The results of these studies were as follows; 1. The growth of M. nanum & T. rubrum was slightly inhibited by PFWE $500\;{\mu}g/ml$, and the growth of M. nanum, M. cookei & T. rubrum was slightly inhibited by PFWE $1,000\;{\mu}g/ml$. The growth of E. floccosum, M. gypseum & M. cookei was slightly inhibited, however the growth of M. canis, M. nanum, T. mentagrophytes, T. rubrum & T. tonsurans was significantly inhibited by PFWE $5,000\;{\mu}g/ml$. With $500\;{\mu}g/ml$ of PFEE, the growth of M. canis, M. nanum, T. mentagrophytes, T. rubrum & T. tonsurans was significantly inhibited, and moderate inhibition of M. cookei growth and slight inhibition E. floccosum & M. gypseum were observed. The growth of M. canis, M. nanum, T. mentagrophytes, T. rubrum & T. tonsurans was significantly inhibited, and the growth of E. floccosum, M. gypseum & M. cookei was moderately inhibited by PFEE $1,000\;{\mu}g/ml$. Significant inhibitions of the growth of E. floccosum, M. canis, M. nanum, M. gypseum, M. cookei, T. mentagrophytes, T. rubrum & T. tonsurans were observed by PFEE $5,000\;{\mu}g/ml$. 3. The growth of E. floccosum & M. cookei was moderately inhibited, and the growth of M. canis, M. nanum, M. gypseum, M. mentagrophytes, T. rubrum & T. tonsurans was significantly inhibited by PFME $500\;{\mu}g/ml$. But the growth of E. floccosum, M. canis, M. nanum, M. gypseum, M. cookei, T. mentagrophytes, T, rubrum & T. tonsurans was significantly inhibited, and the growth of T. verrucosum was slightly inhibited in both PFME $1,000\;{\mu}g/ml$ and $5,000\;{\mu}g/ml$.

• Korean Journal of Veterinary Research
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• v.42 no.3
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• pp.363-370
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• 2002

For the development of diagnostic polymerase chain reaction (PCR) to fungal infection by dermatophytes Trichophyton and Microsporum, detection of the fungal DNA by PCR and analysis of the DNA pattern were undertaken in the present study. A total of 15 strains were tested and those consisted of 3 reference strains and 12 isolates such as: reference strains of T mentagrophytes (downy type, ATCC 9533), T rubrum (IFO 6204) and M gypseum (ATCC 9083), and each isolate of T mentogrophytes (powdery type), T mentagrophytes (granular type), T mentogrophytes (purple-red type), T rubrum, T raubitschekii, T tonsurans, T equinum, T ajelloi, T verrucosum, M cookei, M nanum and M gypseum. The DNA were purely isolated from all strains of Trichophyton spp. and Microsporum spp. by a simple method partly consisted of disruption of fungal cells by lyophilization and grinding and extraction of fungal DNA without phenol treatment which is a routine procedure in DNA isolation. For the detection of fungal DNAs, optimal condition of PCR was determined as preheating once at $94^{\circ}C$ for 5 min, 35 cycles of denaturation at $94^{\circ}C$ for 1 min, annealing at $38^{\circ}C$ for 1 min and polymerization at $72^{\circ}C$ for 2 min, and 1 cycle of final extension at $72^{\circ}C$ for 5 min. In PCR using arbitrary primers AP-1 (5' ACCCGACCTG3') and AP-2 (5' ACGGGCCAGT3'), DNAs in various numbers and sizes were detected from different species of Trichophyton and Microsporum, while DNAs in similar size were also detected in all strains of Trichophyton spp. and Microsporum spp. There were unique DNAs observed from certain dermatophytes by AP-1 such as 1,900 bases in T rubrum, 950 and 1,100 bases in T raubitscheldi, 2,100 bases in T equinum, 400 bases in T verrucosum and 1,150 bases in M gypseum. The unique DNAs were also observed by AP-2 such as 1,200 bases in T ajelloi, 250 bases in T verrucosum, 1,150 bases in M cookei and 2,000 bases in M nanum. The results indicated that PCR can detect a specific DNA from certain Trychophyton and Microsporum spp, which can be the information for further development of diagoomc PCR to dennatophytes.

• The Korean Journal of Pharmacology
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• v.9 no.2
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• pp.1-6
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• 1973

Although numerous drugs are available for the treatment of superficial fungi infections of skin, the clinical effects of the majority of such drugs are not satisfactory. In the hope of searching the effective drugs for superficial fungi infections, authors studied whether Veratrum rhizoma extracts had any effect on fungi, with water extract (VRWE), ethanol extract (VREE) and methanol extract (VRME) from Veratrum album L. var. grandiflorum Max. In in vitro studies, the spores of fungi were inoculated on Sabouraud's glucose agar media which contained three extracts of Veratrun rhizoma in each concentration of $500\;{\mu}g/ml$, $1,000\;{\mu}g/ml$ and $5,000\;{\mu}g/ml$ respectively, and the growth of the fungi were observed for 3 weeks. The species of the fungi used in these experiments were Epidermophyton floccosum, Microsporum canis, Microsporum nanum, Microsporum gypseum, Microsporum cookei, Trichophyton rubrum, Trichophyton mentagrophytes, Trichophyton tonsurans and Trichophyton verrucosum. The results of the studies were as follows: 1. The growth of M. canis, M. nanum, T mentagrophytes and T. tonsurans were slightly inhibited by VRWE $1,000\;{\mu}g/ml$, and with VRWE $5,000\;{\mu}g/ml$, the growth of E. floccosum, M. gypseum and T. rubrum were slightly inhibited, moderate inhibition on the growth of M. canis, M. nanum, M. cookei, T. mentagrophytes and T. tonsurans were showed by VRWE $5,000\;{\mu}g/ml$. 2. With $500\;{\mu}g/ml$ of VREE, the inhibition on growth of E. floccosum, M. nanum and M. gypseum were slight, however significant inhibition on the growth of M. canis, M. cookei, T. mentagrophytes, T. rubrum and T. tonsurans were observed. The growth of M. nanum and M. gypseum were moderately inhibited, and significant inhibition on the growth of E. floccosum, M. canis, M. cookei, T. mentagrophytes, T. rubrum and T. tonsurans were observed by VREE $1,000\;{\mu}g/ml$. By VREE $5,000\;{\mu}g/ml$, the growth of all tested fungi were significantly inhibited except T. verrucosuia being showed slight inhibition. 3. Significant inhibition on the growth of M. canis, T, mentagrophytes, T. rubrum and T. tonsurans were noted, and moderate inhibition of M. nanum, slight inhibition of E. floccosum and M. gypseum in growth were observed by VRME $500\;{\mu}g/ml$. The growth of E. floccosum, M. canis, M. nanum, M. cookei, T. mentsgrophytes, T. rubrum and T. tonsurans were significantly inhibited by VRME $1,000\;{\mu}g/ml$, and that of M. gypseum was moderate. With $5,000\;{\mu}g/ml$ of VRME, significant inhibition on the growth of E. floccosum, M. canis, M. nanum, M. gypseum, M. cookei, T mentagrophytes, T. rubrum and T. tonsurans were observed, and T. verrucosum was showed only slight inhibition. From the above results, it was found that the extracts of organic solvents from Veratrum rhizoma (VREE & VRME) exerted significant antifungal activity, and their effects were probably derived from the pharmacological action of steroidal alkaloids.

• The Korean Journal of Pharmacology
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• v.9 no.2
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• pp.39-45
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• 1973

The majority of drugs used in the treatment of superficial fungal infections has limited values due to its low efficacy or development of resistance. For the purpose of searching efficacious agent on the superficial fungal infections induced by dermatophytes which is regarded as the most malicious one, authors examined whether Chinae Rhizoma Extracts have significant on it. Extracts from Smilax china Linne used for the study are water extract (CRWE), ethanol extract (CREE) and methanol extract (CRME). In in vitro studies, the spores of the dermatophytes were inoculated on Sabouraud's glucose agar media which contained three extracts of Chinae Rhizoma in each concentration of $500\;{\mu}g/ml$, $1,000\;{\mu}g/ml$ and $5,000\;{\mu}g/ml$ respectively, and also $1,000\;{\mu}g/ml$ of salicylic acid and undecylenic acid $1,000\;{\mu}g/ml$ as comparable drugs. The growth of the dermatophytes were observed for 3 weeks. The species of the dermatophytes used in this experiment were Epidermophyton floccosum, Microsporum canis, Microsporum cookei, Microsporum gypseum, Microsporum nanum, Trichophyton mentagrophytes, Trichophyton rubrum, Trichophyton tonsurans and Trichophyton verrucosum distributed from The Institute of Tropical Medicine in Belgium. The results of the studies were as follows: 1. The growth of M. canis, M. nanum, T. mentagrophytes, T. rubrum & T. tonsurans were slightly inhibited in CRWE $1,000\;{\mu}g/ml$ and CRWE $5,000\;{\mu}g/ml$, and only slight inhibition on the growth of E. floccosum, M. canis and M. gypseum were observed in CRWE $5,000\;{\mu}g/ml$. 2. Complete inhibition of T. rubrum, moderate inhibition of M. nanum & T. tonsurans, and slight inhibition of E. floccosunl, M. canis, M. cookei & T. mentagrophytes in growth were observed in concentration of CREE $500\;{\mu}g/ml$. The growth of M. gypseum was slightly inhibited, moderate inhibition on the growth of M. canis, M. cookei & T. mentagrophytes, and complete inhibition of E. floccosum, M. nanum, T. rubrum & T. tonsurans in growth were observeed by CREE $1,000\;{\mu}g/ml$. With $5,000\;{\mu}g/ml$ of CREE, the growth of E. floccosum, M. canis, M. cookei, M. gypseum, T. mentagrophytes, T. rubrum & T. tonsurans were completely inhibited except T. verrucosum being showed slight inhibition. 3. In CRME $500\;{\mu}g/ml$, slight inhibition of T. verrucosum, moderate inhibition of M. gypseum and complete inhibition of E. floccosum, M. canis, M. cookei, T. mentagrophytes, T. rubrum & T. tonsurans in growth were observed. The growth of E. floccosum, M. canis, M. cookei, M. gypseum, M. nanum, T. mentagrophytes, T. rubrum & T. tonsurans were completely inhibited except T. verrucosum being showed moderate inhibition in both CRME $1,000\;{\mu}g/ml$ and CRME $5,000\;{\mu}g/ml$. 4. In $1,000\;{\mu}g/ml$ of undecylenic acid, slight inhibition of T. verrucosum and complete inhibition of E, floccosum, M. canis, M. cookei, M. gypseum, M. nanum, T. mentagrophytes, T. rubrum & T. tonsurans in growth were observed. From the above results, it was found that Chinas Rhizoma Alcoholic Extracts(CREE & CRME) exerted significant antifungal activity, and their effects were probably derived from the pharmacological actions of triterpenoidal saponin and steroidal saponin.

• Korean Journal of Clinical Laboratory Science
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• v.50 no.4
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• pp.444-448
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• 2018

From January 2 to December 30, 2017, out of 691 children diagnosed with dermatophytoses infection, 179 children had infections caused by Trichophyton among the total 13,093 patients who visited the Dermatology department. According to a survey, the patients included 317 adolescents, 203 elementary school age children, and 171 infants. In seasonal variation, the diagnosis was higher in summer (195 cases, 28%) and winter (191, 28%) and comparatively lower in spring (165, 24%) and autumn (140, 20%). The infection among the patients was caused by, from maximum to minimum, T. pedis (351, 51%), T. corporis (91, 13%), and T. unguinum (77, 11%). In all age groups, the highest number of patients were infected with T. pedis. After T. pedis, in descending order, the infection was caused by T. corporis, T. unguinum, T. manus and Tinea capitis in the infants and elementary school age children and by T. corporis, T. unguinum and T. cruris in adolescents. Among infections caused by Trichophyton, T. rubrum was the highest isolated causative agent. This data analysis confirmed the distribution of dermatophytoses and the different species according to paediatric age and will aid the study of paediatric dermatomycosis through continuous research.

• The Korean Journal of Mycology
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• v.21 no.1
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• pp.77-84
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• 1993

Out of 210 tinea capitis cases studies, 16 were negative when examined with KOH, among the remaining 194 cases, 123 were males (63.4%) and 71 were females (36.6%) and the age of incidence ranged between 7 and 15 years. Microsporum was the main causal agent being identified in 82 cases (42.3%) and was represented by 5 species among which M. canis was common in Egypt (55 cases, 28.4%). Trichophyton constituted 32% and was represented by 8 species among which T. violaceum was the most common (24.2% of total cases). Candida were isolated from 3.6% of total cases. The 47 species and twenty-five genera from nondermatophyte-cyclohexamide resistant fungi were recovered from the diseased skin tissue. Penicillium and Aspergillus were the most abundant followed by Scopulariopsis, Alternaria, Thermoascus, Chrysosporium and Cladosporium. Studies of the air-borne fungi in-door the hospital wards revealed the occurrence of 57 species belonging to 28 genera, among which Aspergillus flavus, Penicillium chrysogenum, p. corylophilum, A. niger, Tritirachium rosum and Alternaria alternata were the most common. Results of the out-door experiments were basically similar to those of the in-door experiments.

• Korean Journal of Clinical Laboratory Science
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• v.49 no.4
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• pp.439-445
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• 2017

In recent years, change in life patterns gave rise to an increase in the number of families with companion animals, and as a result, frequent dermatophytes infections have been reported. Microsporum canis, Trichophyton mentagrophytes, and Trichophyton verrucosum, are among these species of zoophilic dermatophytes. Trichophyton mentagrophytes are transmitted to humans by contact with wild animals. Infection from it causes strong inflammation in humans. Conversely, Trichophyton verrucosum is transmitted by contact with cattles. Microsporum canis will become latent carriers in cats or dogs, causing infectious diseases when it comes in contact with humans. We investigated zoophilic dermatophytes isolated according to annual, sex, age, season, body sites, and clinical types between 2010 and 2016. According to our results, the isolation rate of zoophilic dermatophytes was 0.37%, among which, 88 T. mentagrophytes, 228 Microsporum canis, and 18 Trichophyton verrucosum were isolated in human. It is interesting to note that Microsporum canis has been on the rise since 2014. Microsporum canis and Trichophyton verrucosum were highly isolated in females, but T. mentagrophytes was isolated similarly in both sexes. According to an age-based survey, the isolation rate was higher in children younger than 10 years. Our results is a valuable data for predicting and studying the isolation of zoophilic dermatophytes in the future.

• Mycobiology
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• v.30 no.3
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• pp.154-159
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• 2002

Fifty cases of dermatomycoses were recorded from adult male and female at Qena Gvernorates. These included tinea capitis(62% of total cases), tinea corporis(20%), tinea versicolor(12%) and tinea unguium(6%). Males are more susceptible to all cases of tinea than females. Thirty-one species and 2 varieties belonging to 16 genera were recovered from several infection sites. These were identified as Aphanoascus fulvescens, A. terreus, Arthroderma fulva, A. obtusa, Trichophyton rubrum and T. soudanense. Several saprophytes were also found. These were : Aspergillus flavus, A. fumigatus, A. niger, A. terreus, Cochliobolus lunatus, Mycosphaerella tassiana, Penicillium chrysogenum and P. citrinum. Twenty-one isolates were able to hydrolyze gelatin with variable capabilities. T. rubrum was the most active protease producer. The maximum production of protease was obtained at 8 days of incubation at $30^{\circ}C$ in Sabouraud's basal medium with maltose as a carbon source and pepton as a nitrogen source. The optinal pH for the maximum production of protease was pH 6.

• Proceedings of the Korean Fiber Society Conference
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• 1996.10a
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• pp.500-506
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• 1996

To study antimicrobial activities of Impatiens balsamina, its leaf and stem was extracted by methanol and water. The extracts were tested their microbial inhibition activities against several microorganisms, staphylococcus aureus KCTC 1928, Escherichia coli KCTC 1116, Klebsiella pneumoniae KCTC 2690, Trichophyton mentagrophytes KCTC 6316. The methanol extract and water extract of the leaf and stem didn't show the inhibition effect on E. coli but showed the inhibition effect against the ohter 3 species of the microorganisms tested. The extract of leaf showed higher inhibition than the extract of stem. And the methanol extract showed higher inhibition than the water extract.

• Korean Journal of Clinical Laboratory Science
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• v.37 no.2
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• pp.118-122
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• 2005

An epidemiological study was performed on zoophilic dermatophytes. The number of patients with zoophilic dermatophytes diagnosed on clinical findings and culture at the Catholic Dermatological Clinic in Daegu City were 1,339 for 10 years from January 1995 to December 2004. Species of zoophilic dermatophytes isolated from 47,925 patients with dermatophytes were as follows; Trichophyton(T.) rubrum was isolated from 43,702 (91.2%), T. interdigitale 2,660 (5.6%), Epidermophyton(E.) floccosum 43 (0.1%), T. tonsurans 131 (0.3%), T. mentagrophytes 625 (1.3%), T. verrucosum 56 (0.2%), Microsporum(M.) canis 658 (1.4%), and M. gypseum 50 (0.1%). Zoophilic dermatophytes infections were chiefly Tinea facial in the cases of T. mentagrphytes and Tinea corporis in the cases of T. verrucosum, but Tinea capitis in the cases of M. canis. Number of patients with zoophilic dermatophytes by site and age were as follows; Most patients with infected scalps were young people under 15 years old. Infection of the trunk area (neck,chest,back) and extremities (arms, legs, hands, feet) were relatively high in those patients over 15 years old. M. canis were most frequently isolated in autumn and winter, and T. mentagrophyts and T. verrucosum were mostly in spring and winter.