• Applied Biological Chemistry
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• v.25 no.4
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• pp.252-256
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• 1982

To investigate the influences of biological factors on stored rice and their interactions, the activities of red Hour beetle(Tribolium castaneum) and 2 species of Aspergillus was observed during 6 weeks at $28^{\circ}C{\pm}1^{\circ}C$. It was found that the red flour beetle could complete its life cycle on rice in six weeks. Its activity caused the increse of moisture content of rice(max. 1.3%) after 6 weeks, but the number of mold propagule on the surface of stored rice decreased. The growth of red flour beetle showed a stabilizing tendency on rice inoculated with Asp. repens while it was retarded with Asp. niger.

• Korean journal of applied entomology
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• v.49 no.4
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• pp.363-369
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• 2010

A postharvest treatment called CATTS (controlled atmosphere and temperature treatment system) has been used as an alternative nonchemical measure for methyl bromide fumigant treatment. This study applied CATTS to control the red flour beetle, Tribolium castaneum, infesting stored grains. Adults of T. castaneum were susceptible to $46^{\circ}C$ heat treatment. The susceptibility was further enhanced by addition of CA conditions (15% $CO_2$ and 1% $O_2$). When CATTS ($46^{\circ}C$, 15% $CO_2$, $16^{\circ}C/h$ treating rate) was applied to different developmental stages of T. castaneum, it showed 100% control efficacy by 120 min exposure. There was a variation in CATTS susceptibility among developmental stages, in which late instar larvae were most tolerant. Heat shock proteins of T. castaneum appeared to be implicated in the tolerance of CATTS.

• International Journal of Industrial Entomology and Biomaterials
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• v.2 no.1
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• pp.55-59
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• 2001

The vitellin of the red flour beetled Tribolium castaneum Herbst was purified and characterized. The vitellin of T. castaneum was purified by the FPLC techniques, anion exchange chromatography and gel permeation chromatography. In native-polyacrylamide gel electrophoresis, vitellin of T. castaneum was detected as a single band. This native vitellin has molecular weight of 440 kDa. The vitellin of T. castaneum is composed of three polypeptides, designated Vnl (178 kDa), Vn2 (168 kDa) and Vn3 (52 kDa) in SDS-polyacrylamide gel electrophoresis. Three subunits of vitellin were presented in the female adult hemolymph and egg extracts, but not observed in the male. These three polypeptides gradually decreased during embryogenesis. Polyclonal antiserum raised against purified vitellin reacted with the three polypeptides, Vnl, Vn2 and Vn3. Antisera raised against Vn1 and Vn2 cross-reacted with the two large subunits, Vnl and Vn2, respectively. Another subunits Vn3, however, was not cross-reacted with these two antisera. Also, antiserum raised against Vn3 did not cross-react with the Vn1 and Vn2.

• Journal of Applied Biological Chemistry
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• v.59 no.2
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• pp.99-102
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• 2016

The phototactic behavioral responses of Tribolium castaneum adults to light-emitting diodes (LEDs) of seven different wavelengths were determined under various conditions (light exposure times, light sources, and luminance intensities) and compared with those of a black light bulb (BLB) under laboratory conditions. Based on the attractive rate (%) of T. castaneum adults under optimal conditions (50 lx and an 48 h exposure time) in the dark, red LED ($625{\pm}10nm$) exhibited the highest potential attractive rate (97.8 %), followed by yellow ($590{\pm}5nm$, 68.9 %), green ($520{\pm}5nm$, 55.6 %), infrared (IR) (730 nm, 54.4 %), white (450-620 nm, 41.1 %), blue ($470{\pm}10nm$, 34.4 %), and ultraviolet (UV) (365 nm, 0.06 %) LEDs. In comparison, red LED (97.8 %) was approximately 3.4 times more attractive to T. castaneum adults than the BLB (28.9 %). These results indicate that a red LED trap could be useful to control T. castaneum adults.

• Advances in nano research
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• v.17 no.1
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• pp.75-86
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• 2024

The insecticidal efficiency of orange (Citrus sinensis) peel essential oil (OP-EO) is limited because of its low stability under environmental conditions. Nanoemulsion formulations show promise in overcoming this limitation. Therefore, this study aimed to formulate and characterize the OP-EO nanoemulsion form (OP-EON) and investigate its insecticidal properties against two significant storage pests, Rhyzopertha dominica (Fabricius, 1792), and Tribolium castaneum (Herbst, 1797). The OP-EON (4:3:3:90 w/w, EO: Tween 80: Ethanol: water) was successfully created using an ultrasonic homogenizer. The major chemical components of the OP-EO were determined to be D-limonene (87.93%), myrcene (3.62%), and α-pinene (1.34%) through GC-MS analysis. The OP-EON was characterized using TEM (50-100 nm), Zeta sizer (the mean droplet particle size of 58.60 nm, the ζ-potential value of -12.6 mV, and the polydispersity index of 0.486), and FT-IR analysis. After 7 days, exposure to 500 ppm of the OP-EON resulted in 50% and 30% mortality rates in R. dominica and T. castaneum, respectively. Exposure to 1000 ppm of OP-EON resulted in 90% and 55% mortality in R. dominica and T. castaneum, respectively, after 7 days. Overall, these results clearly showed the potential to exceed the limits of the insecticidal activity of the OP-EO with its nanoemulsion form.

• The Korean Journal of Pesticide Science
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• v.20 no.4
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• pp.355-360
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• 2016

The insecticidal activities of the essential oils of Artemisia vulgaris, Achillea millefolium, Anethum graveolens, Cimicifuga heracleifolia, Citrus paradisi, Chrysanthemum indicum, Eucalyptus dives, Neroli bigard, Nigella sativa, and Ruta graveolens against stored-grain insects, Plodia interpunctella and Tribolium castaneum larvae and adults were evaluated using fumigant and contact bioassays. Based on the $LD_{50}$ values in fumigant bioassay, A. millefolium ($0.12{\mu}g/cm^3$) and A. graveolens ($93.14{\mu}g\;cm^{-3}$) oil were most effective against P. interpunctella and T. castaneum adults. In addition, N. bigard ($139.11{\mu}g\;cm^{-3}$) and A. graveolens ($52.87{\mu}g\;cm^{-3}$) oil exhibit strong insecticidal activities against P. interpunctella and Tribolium castaneum larvae in contact bioassay. These results indicate that A. millefolium, A. graveolens, N. bigard, and A. graveolens oils could be effective natural acaricides for managing stored-grain insects, P. interpunctella and T. castaneum.

• Journal of Applied Biological Chemistry
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• v.60 no.1
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• pp.69-71
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• 2017

The insecticidal activities of the Eucalyptus dives and Thymus vulgaris oils were assessed against Plodia interpunctella and Tribolium castaneum in granary. Insecticidal activities of the E. dives oil showed 100 % mortality against P. interpunctella larvae for 72 h and P. interpunctella adults for 3 h. Moreover, the T. vulgaris oil was observed with 100 % mortality against T. castaneum larvae for 120 h and T. castaneum adults for 120 h. These results indicate that it may be possible to control P. interpunctella and T. castaneum through the application of E. dives and T. vulgaris oils in granary.

• The Plant Pathology Journal
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• v.34 no.5
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• pp.356-366
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• 2018

The red flour beetle, Tribolium castaneum, is one of the most common and economically important pests of stored cereal products worldwide. Furthermore, these beetles can act as vectors for several fungal post-harvest diseases. In this study, we collected T. castaneum from 49 rice processing complexes (RPCs) nationwide during 2016-2017 and identified contaminating fungal species on the surface of the beetles. Five beetles from each region were placed on potato dextrose agar media or Fusarium selection media after wet processing with 100% relative humidity at $27^{\circ}C$ for one week. A total of 142 fungal isolates were thus collected. By sequence analysis of the internal transcribed spacer region, 23 fungal genera including one unidentified taxon were found to be associated with T. castaneum. The genus Aspergillus spp. (28.9%) was the most frequently present, followed by Cladosporium spp. (12.0%), Hyphopichia burtonii (9.2%), Penicillium spp. (8.5%), Mucor spp. (6.3%), Rhizopus spp. (5.6%), Cephaliophora spp. (3.5%), Alternaria alternata (2.8%) and Monascus sp. (2.8%). Less commonly identified were genera Fusarium, Nigrospora, Beauveria, Chaetomium, Coprinellus, Irpex, Lichtheimia, Trichoderma, Byssochlamys, Cochliobolus, Cunninghamella, Mortierella, Polyporales, Rhizomucor and Talaromyces. Among the isolates, two known mycotoxin-producing fungi, Aspergillus flavus and Fusarium spp. were also identified. This result is consistent with previous studies that surveyed fungal and mycotoxin contamination in rice from RPCs. Our study indicates that the storage pest, T. castaneum, would play an important role in spreading fungal contaminants and consequently increasing mycotoxin contamination in stored rice.

• Journal of Applied Biological Chemistry
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• v.59 no.2
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• pp.129-132
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• 2016

This study was conducted to evaluate the attraction effects of Sitophilus zeamais and Tribolium castaneum to light emitting diode (LED) trap in granary and compared with the black light bulb (BLB) trap, which is typical used in commercial trap. The red LED trap showed more attractive to S. zeamais and T. castaneum than that of the BLB. Moreover, the external condition of granary was about 1.5 times more attractive to S. zeamais and T. castaneum than the internal condition of granary. These results suggested that red LED trap could be useful to control S. zeamais and T. castaneum in granary.

• Proceedings of the Korean Society of Sericultural Science Conference
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• 2000.10a
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• pp.133-133
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• 2000