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Isolation and characterization of cellulolytic yeast belonging to Moesziomyces sp. from the gut of Grasshopper (메뚜기의 내장에서 분리한 Moesziomyces 속에 속하는 셀룰로오스 분해 효모의 분리 및 특성)

  • Kim, Ju-Young;Jung, Hee-Young;Park, Jong-Seok;Cho, Sung-Jin;Lee, Hoon Bok;Sung, Gi-Ho;Subramani, Gayathri;Kim, Myung Kyum
    • Korean Journal of Microbiology
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    • v.55 no.3
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    • pp.234-241
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    • 2019
  • An intensive interaction between yeasts and insects has highlighted their relevance for attraction to food and for the insect's development and behavior. Yeast associated in the gut of insects secretes cellulase which aided in the food digestion (cellulose degradation). Three strains of cellulose-degrading yeast were isolated from the gut of adult grasshoppers collected in Gyeonggi Province, South Korea. The strains $ON22^T$, $G10^T$, and $G15^T$, showed positive cellulolytic activity in the carboxymethyl cellulose (CMC)-plate assay. The phylogenetic tree based on sequence analysis of D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer (ITS) regions revealed that the strains $ON22^T$ (100 and 98.4% sequence similarities in D1/D2 domains and ITS) and $G10^T$ (99.8 and 99.5% in D1/D2 domain and ITS region) were most closely related to the species Moesziomyces aphidis JCM $10318^T$; $G15^T$ (100% in D1/D2 domains and ITS) belongs to the species Moesziomyces antarcticus JCM $10317^T$, respectively. Morphology and biochemical test results are provided in the species description. Cellulase with its massive applicability has been used in various industrial processes such as biofuels like bioethanol productions. Therefore, this is the first report of the cellulolytic yeast strains $ON22^T$, $G10^T$, and $G15^T$ related to the genus Moesziomyces in the family Ustilaginaceae (Ustilaginales), in Korea.

A Study on the Landscape Planning and Landscape Architecture Construction Principles by the Type Outside Relics (발굴유적 외부공간의 유형별 경관계획 및 조경시공 원칙 연구)

  • Shin, Hyun-Sil
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.36 no.4
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    • pp.58-69
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    • 2018
  • The purpose of this study was to present specific standards for landscape planning and construction applicable to landscaping areas in various historical sites. The results are as follows: First, the types of historical sites are classified according to their preservation status and processing techniques, and the classification according to their status is divided into those that have been buried underground, such as those that have been exposed to the ground, such as buildings and structures, and those that have a reputation or a record of the sites. The classification according to the wartime processing technique was classified by such techniques as closures that isolate the site, penetration that can be seen on the site, and overlays where a separate protection facility is installed on the site. Second, the principles of landscape planning for display of historical sites are divided into the items that make up the historical site, surface treatment of the sites, tree planting, and installation of facilities. If the site is not a circular space, the area separating the space by different materials between the components is required. In the event that preservation of the historical site is required, it is deemed desirable to use the soil, and to do so, the use of grasses and shrubs is effectively considered to be effective. The introduction of plants and plants should be considered according to the nature of the space. Depending on the area and nature of the monument, the facility is required to take a cautious approach by reviewing its influence on the landscape and the additional excavation of the monument. Third, the readjustment method derived as a result of looking at the landscaping principle in the historical site space was classified as conservation of status, installation of protection facilities, burial, restoration, relocation, and reproduction. Preservation of the status quo is essential for limited landscape planning and should not affect the prototype of existing relics. The protection facility shall be installed where necessary to protect the relics, and when the soil is formed, the surface treatment shall be required to remove trees that could damage the site and prevent soil and soil oil from being lost after the site. The restoration shall establish a landscaping plan according to the circular preservation based on the clues to the circle. The transfer requires a landscaping plan to create an environment similar to the outer space of an existing site and should be able to highlight the value or location of the original site. The reenactment should have a landscaping plan to revive the landscape and atmosphere of the past for the now-defunct remains. Fourth, landscaping can simultaneously satisfy the preservation of excavation sites and the increase in exhibition effects. In order to protect the traces of the past and vitalize the site of the ruins today, specific measures are required, the creation of a park for historical sites that preserve the functions and value of the relics, and the formation of a shape of linked contents can be suggested as alternatives.

A Study on the Evaluation and Maintenance for Alternative Habitats of the Narrow-mouth Frog (Kaloula borealis) - A Case Study on the Alternative Habitats of Kaloula borealis at the University of Seoul - (맹꽁이 대체서식지 조성 평가 및 유지관리 방안 연구 - 서울시립대학교 맹꽁이 대체서식지를 사례로 -)

  • Park, Seok-Cheol;Han, Bong-Ho;Park, Min-Jin
    • Journal of the Korean Institute of Landscape Architecture
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    • v.47 no.1
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    • pp.76-87
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    • 2019
  • The purpose of this study was to evaluate the performance of and to derive future maintenance-management measures of the constructed alternative habitat for the Kaloula borealis at the University of Seoul, examining the period between 2015-2017. The research was constructed in 2014 and in a $191m^2$ area. The performance evaluation was divided into maintaining the habitat of the target species, maintaining the population and reproduction rates of the target species, maintaining the habitat of the wild species, the resilience of natural ecosystems, and the harmony with the surrounding environment. In terms of maintaining the habitat of the target species, soil collected from the existing habitat of the Kaloula borealis and was the depth was increased to 30cm in the alternative habitat. An artificial water supply was required every year during the supporting the spawning and hatching of other amphibians along with the Kaloula borealis. The sources of water of the alternative habitat were both rain and tap water, as it cannot be maintained naturally. Additionally, the Kaloula borealis thrived because it inhabited the research site and the average temperature was $26.2^{\circ}C$ from April-June, which is when the Kaloula borealis spawns. In terms of maintaining the population and reproduction rates of the Kaloula borealis, they were evaluated to have stable rates of reproduction. In terms of maintaining the habitat of the wild species, studies on vegetation and the structure of the characteristics of prey or predators will be needed. Also, alien species, such as Humulus japonicus and Bidens frondosa needed to be removed to maintain the wetland ecosystem of the wild species. In the assessment of the resilience of the natural ecosystems, the mud was monitored, noting the changes in the depth of water, with steps taken to reduce the leakage of water. The mud collected from the Haneul Pond wetland, which is located around the research site was piled up. Also, partial mowing management and the inducement of a natural vegetation colony was required for vegetation management. It was also necessary to create porous spaces, such as old trees and tree branches to create a habitat with hiding places and feeding and spawning places for small organisms. In terms of the harmony with the surrounding environment, the following threat factors needed to be managed: amphibian roadkill by vehicles and pedestrians and artificial draining due to nearby user access. Based on the monitoring results, alternative habitat management measures presented the promoting various waterside structures, in which amphibians can spawn and hide in, managing the water environment consistently, managing the vegetation, focused on the habitat of the wild species, and managing the surrounding environment for the habitat. The creation of an alternative habitat should be managed through monitoring, reflecting the characteristics of the changes in the site. Also continuing efforts are also needed to improve the habitat of the target species.

Vegetation Characteristics of Ridge in the Seonunsan Provincial Park (선운산도립공원의 능선부 식생 특성)

  • Kang, Hyun-Mi;Park, Seok-Gon;Kim, Ji-Suk;Lee, Sang-Cheol;Choi, Song-Hyun
    • Korean Journal of Environment and Ecology
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    • v.33 no.1
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    • pp.75-85
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    • 2019
  • The purpose of this study is to understand the vegetation characteristics of ridges (Gyeongsusan-Seonunsan-Gaeipalsan) in the Seonunsan Provincial Park and to establish reference information for the management of the park in the future. We designated 62 plots with the area of $100m^2$ were installed and analyzed them to investigate the vegetation characteristics. The results of community classification based on TWINSPAN showed seven categories of vegetation communities in the surveyed region: Quercus dentata-Deciduous broad-leaved Community, Quercus variabilis-Pinus thunbergii-Quercus serrata Community, Pinus densiflora Community, Deciduous broad-leaved Community-I, Carpinus tschonoskii-Castanea crenata-Quercus aliena Community, Deciduous broad-leaved Community-II, and Carpinus tschonoskii-Carpinus laxiflora Community. In the vegetation of Seonunsan Provincial Park, coniferous trees such as Pinus thunbergii and Pinus densiflora have been gradually losing their population as part of ecological succession to deciduous broad-leaved trees such as Quercus spp., Carpinus tschonoskii, and Carpinus laxiflora. Moreover, Carpinus turczaninowii, Mallotus japonicus, and others were identified as vegetation reflecting the geographical characteristics of the region neighboring the west coast. The estimated age is 30-60 years, and the oldest tree Pinus densiflora is 63-years old. The index of diversity ($100m^2$) was 0.7942 for Carpinus tschonoskii-Carpinus laxiflora Community, 0.8406 for Carpinus tschonoskii-Castanea crenata-Quercus aliena Community, 0.8543 for Quercus dentata-Deciduous broad-leaved Community, 0.9434 for Quercus variabilis-Pinus thunbergii-Quercus serrata Community, 0.9520 for Deciduous broad-leaved Community-I, 0.9633 for Pinus densiflora Community, and 1.0340 for Deciduous broad-leaved Community-II in the ascending order.

A Study on the Basic Directions for Forest Rehabilitation Programs Considering to Economic and Social Conditions of North Korea (북한의 경제사회적 여건을 고려한 황폐산림복구 기본방향 연구)

  • Park, Kyung Seok;Lee, Seong Youn;Park, So Young
    • Journal of Korean Society of Forest Science
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    • v.100 no.3
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    • pp.423-431
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    • 2011
  • The changes of forest degradation in North Korea have closely been related to political, economic and social conditions at all different times. The deforestation by local people for their livelihood has been accelerated when the recession has been worsened due to the 1990's collapse of socialism and the years of natural disasters, and the fall of the centralized and planned economy system. The serious recession in the 1990's has brought many changes in the North Korean society since the 2000's. Not only the underground economy, but also the market in which personal trades are occurred have been expanded as the distribution system of the planned economy system had fallen. In addition, even many state institutions have also increased timber harvest for export to acquire insufficient foreign currency. Eventually, North Korea felt the limits of utilization of forest resources under socialism then started to seek measures to restore devastated forest, while realizing the need of support from the international society. Therefore, some NGOs of South Korea started to give financial support on building tree nurseries in which seedlings for planting are produced to help the rehabilitation of the degraded forests in North Korea. Therefore, Planning of the basic directions for forest rehabilitation programs considering to economic and social conditions of North Korea are needed based on the successful rehabilitation experience of South Korea in the 1970's. First of all, relationships which was built after collapse of centrally planned economy between districts, businesses and workers must be consider to rehabilitate forests in North Korea. Secondly, due to the nature of forest rehabilitation projects this is very needs voluntary participation of resident for a long time, and then forest rehabilitation projects can create jobs for local resident, they can obtain continuous income on the forest rehabilitation projects field in order to promote resident's work in forest rehabilitation projects. Thirdly, the rate dependence on forests of the residents living must keep the level down by rural development projects going side by side with forest rehabilitation projects. Fourthly, use of exsisting forest management system in North Korea is also needed to ensure administrative power and labor for grand scale plantations in a short period of time. Meanwhile after the success of Forest Rehabilitation, it is very important to improve exsisting forest management system.

A Study on the Tendency of Planting Design of Designer's Gardens in the Suncheon Bay National Garden (순천만국가정원 내 작가 정원 식재 경향 연구)

  • Jung, Bom-Bee;Choi, Jung-Mean
    • Journal of the Korean Institute of Landscape Architecture
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    • v.49 no.1
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    • pp.70-82
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    • 2021
  • The purpose of this study is to reveal the tendency of planting design through the analysis of the planting of designer's gardens in the Suncheon Bay National Garden and to derive implications for future garden planting designs. The results of the study are as follows: First, the results of the study show that the practice of tree-based planting is still valid. Large growing trees such as 'Pinus densiflrora', 'Celtis sinensis', 'Zelkova serrata', 'Machilus thunbergii', 'Pinus strobus' overwhelmed the size of the designer's garden(150 to 390㎡). Second, the selection of trees tended to be made considering the designer's intention and the decorative effects rather than by considering the physiological and ecological conditions of the site. Third, among the herbaceous, the rate of the planting of perennials was high. Fourth, the flowering period of planted herbaceous was the most common in summer, followed by spring, fall, and winter. Fifth, the frequency color of the planted herbaceous was the most common in summer, followed by spring, fall, and winter. Fifth, in terms of flower color frequency, the most common was the yellow-series, followed by red-series, blue-series Sixth, average height herbaceous plants(20~60cm) were planted the most(47.4%). Seventh, structural plants that determined the garden's framework depended on trees, and the focal plants mainly utilized were evergreen trees, and the midrange plants were the planted herbaceous plants. The implications derived from the above findings are as follows: First, to ensure the garden's quality and sustainability, the selection of trees should be carefully considered, not considering only the artist's intention but also taking into account the physical and ecological conditions. Second, herbaceous plants can be used in various ways― the garden's focal plants, midrange plants, and ground covers, so more active herbaceous planting needs to be considered. Third, in consideration of the winter landscape, herbaceous planting using characteristics, such as fruits and stems, as well as flower colors should be considered. Fourth, blue and black color herbaceous plants have a noticeable effect even in a small amount, so it is necessary to plant them actively. Fifth, for the design of herbaceous planting, where the individual property of plants can be expressed, the design method should be considered.

Production and Fuel Properties of Wood Chips from Logging Residues by Timber Harvesting Methods (목재수확 방법에 따른 벌채부산물 목재칩의 생산 및 연료 특성)

  • Choi, Yun-Sung;Jeong, In-Seon;Cho, Min-Jae;Mun, Ho-Seong;Oh, Jae-Heun
    • Journal of Korean Society of Forest Science
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    • v.110 no.2
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    • pp.217-232
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    • 2021
  • This study calculated the productivity and cost of extraction and processing of logging residues by cut-to-length (CTL) and whole-tree (WT) harvesting methods. In addition, the comparative analysis of the characteristics of wood chip fuel to examine whether it was suitable for the fuel conditions of the energy facility. In the harvesting and processing system to produce the wood chips of logging residues the system productivity and cost of the CTL harvesting system were 1.6 Gwt/SMH and 89,865 won/Gwt, respectively. The productivity and cost of the WT harvesting system were 2.9 Gwt/SMH and 72,974 won/Gwt, respectively. The WT harvesting productivity increased 1.3times while harvesting cost decreased by 18.7% compared to the CTL harvesting system. The logging residues of wood chips were not suitable for CTL wood chips based on International Organization for Standardization (ISO 17225-4:2021) and South Korea standard (NIFoS, 2020), but the quality (A2, Second class) was improved through screening operation. The WT-unscreened wood chips conformed to NIFoS standard (second class) and did not conform to ISO but were improved through screening operation (Second class). In addition to the energy facility in plant A, all wood chips except CTL-unscreened wood chips were available through drying processing. The WT-unscreened wood chips were the lowest at 99,408 won/Gwt. Plants B, C, and D had higher moisture content than plant A, so WT-unscreened wood chips without drying processing were the lowest at 57,204 won/Gwt. Therefore, the production of logging residues should improve with operation methods that improve the quality of wood chips required for applying the variable biomass and energy facility.

A Study on Prototype Landscape of Mujang-Eupchi(茂長邑治) during Joseon Dynasty (조선시대 무장읍치(茂長邑治)의 원형경관 고찰)

  • Sim, Soon-hee;Song, Suk-ho;Kim, Choong-sik
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.40 no.1
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    • pp.1-14
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    • 2022
  • This study focused on examining the location characteristics of Mujang-Eupchi(茂長邑治), a traditional city of Joseon Dynasty, and shedding light on its prototype landscape. The findings were summarized as follows: Mujang-Eupchi showed a Confucian space system with Munmyo(文廟) within Hyanggyo(鄕校) in the east, Sajikdan(社稷壇) in the west, Seonghwangsa(城隍祠) in the fortress and Yeodan(厲壇) and Seonghwangdan(城隍壇) in Jinsan(鎭山) in the north around the Mujang-Eupseong(茂長邑城), an old fortress, built in the 17th year of King Taejong(1417). It seemed that Seonghwangdan located in Jinsan maintained a coexistence system with Seonghwangsa(城隍祠) within the Eupseong. A Pungsu(風水) stream in a V-shape ran before the southern gate of Eupseong, forming a Sugu(水口) in front of Namsan(南山) that was an Ansan(案山). They dug a southern pond called Hongmunje(紅門堤) to protect the vitality of the village and built Gwanpungjeong(觀豊亭). In the 19th century, Hongmunje and Gwanpungjeong were renamed into Muheungje(茂興堤) and Muheungdang(茂興堂), respectively. Eupsu(邑藪) were planted in front of the southern pond including Wondo(圓島), and Songdeokbi(頌德碑), Dangsanmok(堂山木), and Dangsanseok(堂山石) served as a Sugumagi(水口막이) and protected the entrance of Eupchi. After the Liberation, the southern pond was buried in 1955, and a market was formed at the site, which resulted in the disappearance of its prototype. The study also investigated the name and location of Chilgeori(七거리) in the village as it was lost following the unification of Bu(府), Gun(郡), and Myeon(面) titles in 1914 during the Japanese colonial period. Chilgeori Dangsan was based on Yin and Yang theory and became the subject of the organization mainly composed of Grandfather Dangsan menhir and Grandmother Dangsan tree. Chilgeori Dangsan was a religious place of the community to guard the village, serving as seven gateways to control access at the village boundary and it had a locational feature of protecting the inner mountain ranges of Eupchi.

Local, Jobless Person, Homo Economicus, Three Axis of Kwak Hashin's Works (로컬, 룸펜, 경제적 인간, 곽하신 소설의 세 좌표)

  • Kim, Yang-Sun
    • Journal of Popular Narrative
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    • v.26 no.3
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    • pp.161-188
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    • 2020
  • This paper seeks to expand the scale of literary history by restoring and analyzing the whole aspect of Kwak Hashin's works, which has so far been studied little. For this purpose, I notice the rupture of discontinuity of his works which is greatly divided into the colonial period and post Korean war period. And the characteristics of each works can be analyzed based on the three axis, local(colonial period), jobless person(post-war period), and Homo Economicus(some short stories, and popular novels in post-war period). In Chapter 2, 'Local-the world of Munjang', I evaluated that Kwak Hashin's novel, which had been published in the late 1930s in the Journal of Munjang, embodied anti-modern aesthetic consciousness, as clearly revealing the sorrow for disappearing things, the pre-modern sense of time, and the preference for local. In Chapter 3, 'Jobless Person' and Chapter 4, 'The State of All People's Struggle against All People, The Appearance of Homo Economicus', the Korean society in late 1950s, which entered underdeveloped capitalist countries after Korean war, can be characterized by two contrasting male-gender, one is the jobless, incompetent male, and the economic man on the other hand. In the late '50s, Lumpen(=Jobless Person) novels showed the problems of the Korean economy through incompetent male character. The intelligent men took the path to survival rather than morality or intimacy, projecting their own incompetence and anxiety to women/wives. In the popular novels Women's Song and The Shadow of the Fig Tree, achievement-oriented male figures who betrayed their colleagues, and exploited women's sex by using love relationships to rise to the top appeared. They can be defined as the Homo Economicus who embody the state of universal struggle against all people. These novels showed the formation of the masculinity in post Korean war period, which pursued the survival of the fittest, borrowing form of popular novel. As we have seen so far, Kwak Hashin needs to be re-evaluated as an writer who expanded the modern literary history in the outside of literature. He was the last generation writer written in Korean late colonial period, and provided the model of postwar literature by borrowing the form of journalism and popular novels.

Study on Plant Indicator Species of Picea jezoensis (Siebold & Zucc.) Carrière Forest by Topographic Characters - From China (Baekdu-san) to South Korea - (가문비나무림의 지형특성에 따른 식물 지표종에 관한 연구 - 중국 백두산 일대에서 남한까지 -)

  • Byeong-Joo, Park;Tae-Im, Heo;Jun-Gi, Byeon;Kwang-il, Cheon
    • Journal of Environmental Impact Assessment
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    • v.31 no.6
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    • pp.388-408
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    • 2022
  • This study was conducted to select the indicator species (plant) according to the topographical characteristics in the Picea jezoensis forests, endangered subalpine coniferous trees. In South Korea and China (close to Baekdusan), the southern tree line limit of Picea jezoensis has meaningful geographical and latitudinal values for analyzing the ecological characteristics of P. jezoensis forests. Latitude greatly affects the geographical values of plant ecology, and the difference in latitude and habitat affects the change in species composition in forests. With prolonged environmental change, the habitat of subalpine plants will become smaller, and the plants may become extinct. As the P. jezoensis forests of South Korea and China, in particular, are in danger of disappearing without protection, it is important to monitor the population and develop a conservation strategy. Eighty-seven circular plots were established in P. jezoensis forests in South Korea and China. Through processes such as MRPP-test and NMS ordination, indicator species were selected based on this, and basic data for biodiversity assessment were presented. As a result of the Indicator Species Analysis (ISA), 5 taxa were selected from the upperstory vegetation and 18 taxa from the understory vegetation at the altitude(p<0.05). Indicator species by aspect were analyzed as 3 taxa for upperstory vegetation and 16 taxa for understory vegetation (p<0.05). In the case of indicator species according to the slope, 6 taxa for upper vegetation and 24 taxa for understory vegetation were selected(p<0.05). As for the indicator species according to their habitat, 8 taxa in upper vegetation and 65 taxa on understory vegetation were selected. As a result of MRPP-test, it was analyzed that the species composition was heterogeneous in the group of understory vegetation than that of upperstory vegetation. As a result of NMS ordination, the correlation with environmental factors of indicator species was analyzed by rock exposure for upperstory vegetation and latitude for understory vegetation (cut off level=0.3).