Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Isolation and characterization of cellulolytic yeast belonging to Moesziomyces sp. from the gut of Grasshopper

메뚜기의 내장에서 분리한 Moesziomyces 속에 속하는 셀룰로오스 분해 효모의 분리 및 특성

  • Kim, Ju-Young (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Jung, Hee-Young (College of Agricultural and Life Sciences, Kyungpook National University) ;
  • Park, Jong-Seok (School of Biological Sciences, College of Natural Sciences, Chungbuk National University) ;
  • Cho, Sung-Jin (School of Biological Sciences, College of Natural Sciences, Chungbuk National University) ;
  • Lee, Hoon Bok (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Sung, Gi-Ho (Institute for Healthcare and Life Science, International St. Mary's Hospital and College of Medicine, Catholic Kwandong University) ;
  • Subramani, Gayathri (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Kim, Myung Kyum (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University)
  • 김주영 (서울여자대학교 자연과학대학) ;
  • 정희영 (경북대학교 농업생명과학대학) ;
  • 박종석 (충북대학교 자연과학대학 생물과학부) ;
  • 조성진 (충북대학교 자연과학대학 생물과학부) ;
  • 이훈복 (서울여자대학교 자연과학대학) ;
  • 성기호 (가톨릭관동대학교 국제성모병원 및 의과대학) ;
  • 가야쓰리 수브라마니 (서울여자대학교 자연과학대학) ;
  • 김명겸 (서울여자대학교 자연과학대학)
  • Received : 2019.06.10
  • Accepted : 2019.09.04
  • Published : 2019.09.30
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Abstract

An intensive interaction between yeasts and insects has highlighted their relevance for attraction to food and for the insect's development and behavior. Yeast associated in the gut of insects secretes cellulase which aided in the food digestion (cellulose degradation). Three strains of cellulose-degrading yeast were isolated from the gut of adult grasshoppers collected in Gyeonggi Province, South Korea. The strains $ON22^T$, $G10^T$, and $G15^T$, showed positive cellulolytic activity in the carboxymethyl cellulose (CMC)-plate assay. The phylogenetic tree based on sequence analysis of D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer (ITS) regions revealed that the strains $ON22^T$ (100 and 98.4% sequence similarities in D1/D2 domains and ITS) and $G10^T$ (99.8 and 99.5% in D1/D2 domain and ITS region) were most closely related to the species Moesziomyces aphidis JCM $10318^T$; $G15^T$ (100% in D1/D2 domains and ITS) belongs to the species Moesziomyces antarcticus JCM $10317^T$, respectively. Morphology and biochemical test results are provided in the species description. Cellulase with its massive applicability has been used in various industrial processes such as biofuels like bioethanol productions. Therefore, this is the first report of the cellulolytic yeast strains $ON22^T$, $G10^T$, and $G15^T$ related to the genus Moesziomyces in the family Ustilaginaceae (Ustilaginales), in Korea.

효모와 곤충 간의 집중적인 상호 작용은 곤충의 먹이에 대한 유인과 발달 및 행동에 대한 관련성을 보였다. 곤충 내장에서 분리된 효모는 먹이의 소화를 돕는 셀룰라아제(셀룰로오스 분해)를 분비한다. 한국의 경기도에서 수집한 메뚜기의 장에서 셀룰로오스를 분해하는 효모 세 균주를 분리 하였다. 효모 균주의 cellulase 활성을 확인하기 위해, 카르복시 메틸 셀룰로즈(CMC)를 함유하는 배지로 플레이트상의 투명한 영역을 요오드 용액을 사용하여 관찰하였다. 효모 $ON22^T$, $G10^T$ 및 $G15^T$ 균주는 CMC-플레이트 분석에서 양성 셀룰로오스 활성을 나타냈다. Large subunit rDNA 유전자와 Internal transcribed spacer (ITS) 영역의 D1/D2 영역의 서열 분석에 기초한 계통수를 통해 $ON22^T$와 $G10^T$ 균주가 Moesziomyces aphidis JCM 10318 (D1/D2 영역에서 각 100%와 99.8%, ITS 영역에서 각 98.4% 및 99.5% 서열유사성)와 가장 가깝고 G15는 Moesziomyces antarcticus JCM $10317^T$ 종 (D1/D2 영역에서 100%, ITS에서 100% 서열 유사성)에 속한다는 것을 밝혔다. 셀룰라아제는 바이오 에탄올 생산과 같은 바이오 연료와 같은 다양한 산업 공정에서 사용되고 있다. 따라서, 셀룰로오스 분해 미생물의 분리 및 연구는 중요성을 갖게 되었다. 이 논문은 한국의 Moesziomyces 속의 셀룰로오스 분해 효모 균주인 $ON22^T$, $G10^T$, $G15^T$에 대한 첫 번째 보고이다.

Keywords

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