This study was conducted to elucidate the most appropriate method to obtain auxotrophic mutants from Valsa ceratosperma, the causal fungus of apple canker, which may be used as a gene marker in detecting the transfer of the factors of avirulent strains to virulent strains. Among the 3 kinds of synthetic media tested, each have two formula for minimal and complete, the medium which has been used in study of Endothia parasitica (E. P medium) was turned out to be most appropriate for the growth of V. ceratosperma. A medium for single colony formation from pycnidiospore of this fungus was developed by adding 0.5% L - sorbose to the E. P minimal medium. The period of incubation in dark for preventing the photoreactivation after U. V irradiation was estimated as about 60hrs at which most of the spores become binucleate. Largest number of putative auxotrophs were obtained at about 50second of irradiation to the spores smeared on the medium for single colony formation, at which the survival rate of spores was 5 to 6 percent. With these method developed in this experiment, 161 isolates of putative auxotrophs were detected among which the nutrient requirement for 10 isolates were determined. Five out of 10 mutants were still virulent to apple tree and all but one could not sporulate.
This experiment was to investigate and analyse the effect of fertilizer at different level of treatment where the ordinary chemical fertilizers (Urea, TSP, muriate of potash) and the same amount of nutrients contained solid fertilizer respectively applied. 1. Ordinary chemical fertilizer known as quick releasing fertilizer showed its main effect in the first and second years but the effect was declined in the third year wheras slow releasing solid fertilizer showed no effect in the first year but gradually appeared in second year was reached at peak in the third year and effect continued till the forth year. 2. Both treatments, ordinary chemical fertilizer and solid fertilizer, showed fertilizer effect however ordinary chemical fertilizer treatment had 6.2 per cent of fertilizer loss over the solid fertilizer treatment. 3. Optimum fertilizer dose per tree seems to be 40-80 grams and fertilizer effect was continued for three years after the treatment. Fertilizer response in the first year only appeared on leaves with dark in color and its response on growth was observed in second year where ordinary chemical fertilizer applied and solid fertilizer treatment showed in third year. 4. For the above results, it may be desirable that optimum amount of ordinary chemical fertilizer should be applied once every other or every two years and solid fertilizer should be applied once every two years or every three years. 5. Solid fertilizer does not occure fertilizer damage when it was applied in excess and has labor saving advantage. 6. It is highly recommendable to produce solid fertilizer for the forest use in view of improving forest soil fertility and increasing forest resources in Korea.
Generally potassium in the soil can be remained as water soluble, exchangeable, and available types, respectively. Theses types of potassiums are also known to keep their kinetic relationships to each other. The Purpose on this research was to investigate types and relationships of potassium in the soil, and the characteristics of potassium supply in different forest soils. The results could be summarized as follows; 1. The amounts of available potassium in A and B horizon soils by continuous leaching with 0.01N-HCl were as follows : for A Horizon soils, pohang(Gray brown forest soils), Changsung (Brown forest soils), and Youngwol(Dark red forest soils) were ranged from 0.2me/l00g to 0.8me/100g, Taean(Red and Yellow forest soils) was 0.1-0.6me/100g, Kapyung(Brown forest soils) was 0.2-0.4me/100g. For the B layer, Youngwol was 0.1-0.5me/100g, Pohang, Taean, Kapyung were 0.1-0.4me/100g, Changsung was 0.1-0.3me/100g, respectivly. 2. Of ten times-pulse leaching with 0.01N-HCl for A layer soil, more 80% of total available potassium leaching were recovered by the second pulse as leachate for Pohang, Changsung, Youngwol, while similar amounts of potassium in the leachate were obtained with the third pulse leaching for Kapyung, Taean, respectively, On the other hand, the 80 % release of available potassium from the B layer soil was obtained by the second pulse leaching for all areas investigated. 3. For the relationships between soluble potassium and exchangeable potassium, the exchangeable potassium was increased while the soluble potassium was not changed significantly in B layer. And both soluble potassium and exchangeable potassium in A layer were similar. For the relationship between exchangeable potassium and available potassium in both A and B layers, the amounts of available potassium increased by 1.2 to 1.5 times as the exchangeable potassium increased. 4. For distribution of all types of potassium throughout locations investigated, the types of potassium were in the decreasing orders of available, exchangeable, and soluble. 5. The simplified method for an analysis of all types of potassium by sequential leaching with 0.01N-HCl should be developed not only with respect to time-saving and efficiency but also verification of the relationship between available potassium and tree growth.
Moon, Na Hyun;Shin, Man Yong;Moon, Ga Hyun;Chun, Junghwa
Korean Journal of Agricultural and Forest Meteorology
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제21권3호
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pp.121-134
/
2019
This study was conducted to review the trends of forest meteorological studies based on the publications for last 20 years in Korean Journal of Agricultural and Forest Meteorology (KJAFM), and to provide insight for future prospect for researches in the field of forest meteorology. A total of 220 papers related to forest meteorology were published in KJAFM for the last 20 years. That corresponds to 33.5% out of all the papers including agricultural meteorology papers. To review the trends of forest meteorology studies, the 220 published papers were classified into seven categories. They are forest meteorology and forest fire, forest meteorology and tree physiology, forest meteorology and forest protection, micrometeorology in mountain area, climate and forest growth, climate and forest vegetation distribution, and climate change and forest ecosystem. Even if there were differences in paper numbers among the seven categories, it was found that various and very specific studies were conducted in the field of forest meteorology for the last 20 years. It was also expected that the accumulation and utilization of various and accurate forest meteorological information would bring remarkable progress of forest meteorological studies in the near future.
Cho, Min Seok;Yang, A-Ram;Hwang, Jaehong;Park, Byung Bae;Park, Gwan Soo
Journal of Korean Society of Forest Science
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제110권2호
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pp.198-209
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2021
This study evaluated the effects of the dimensional characteristics of containers on the nitrogen status of Quercus serrata, Fraxinus rhynchophylla, and Zelkova serrata in the container nursery stage. Seedlings were grown using 16 container types [four growing densities (100, 144, 196, and 256 seedlings/m2) × four cavity volumes (220, 300, 380, and 460 cm3/cavity)]. Two-way ANOVA was performed to test the differences in nitrogen concentration and seedling content among container types. Additionally, we performed multiple regression analyses to correlate container dimensions and nitrogen content. Container types had a strong influence on nitrogen concentration and the content of the seedling species, with a significant interaction effect between growing density and cavity volume. Cavity volumes were positively correlated with the nitrogen content of the three seedling species, whereas growing density negatively affected those of F. rhynchophylla. Further, nutrient vector analysis revealed that the seedling nutrient loading capacities of the three species, such as efficiency and accumulation, were altered because of the different fertilization effects by container types. The optimal ranges of container dimension by each tree species, obtained multiple regression analysis with nitrogen content, were found to be approximately 180-210 seedlings/m2 and 410-460 cm3/cavity for Q. serrata, 100-120 seedlings/m2 and 350-420 cm3/cavity for F. rhynchophylla, and 190-220 seedlings/m2 and 380-430 cm3/cavity for Z. serrata. This study suggests that an adequate type of container will improve seedling quality with higher nutrient loading capacity production in nursery stages and increase seedling growth in plantation stages.
Distribution types of native conifers (Juniperus chinensis, Pinus parviflora, Tusga sieboldii and Taxus cuspidata var. latifolia) were studied by phytosociological investigation and ZM method in Ulleung Island, South Korea. Two main types were divided maritime vegetation (Juniperus chinensis forest) and mountain vegetation (Taxus cuspidata var. latifolia forest and Pinus parviflora-Tusga sieboldii forest). The former was divided into sea cliff distribution (J-SC) and sea ridge distribution (J-SR) type. The latter was classified 7 distribution types; Taxus cuspidata var. latifolia forest was rock distribution (Ta-R) and mountain slope distribution (Ta-MS) type, and Pinus parviflora-Tusga sieboldii forest was rock distribution (P T-R), upper and ridge distribution (P T-UR, 3 units sub-types:1sub, 2sub, 3sub), and Mountain slope distribution (P T-MS) type. It was considered that J-SC, Ta- R, and P T-R were maintained by topographic climax, but J-SR, Ta-MS, P T-UR and P T-MS were the process of vegetation succession. Distribution types of topographic climax are entrusted to process of vegetation succession. Types in the process of vegetation succession will be needed tending of forest to promote saplings growth and seedlings germination. Especially in order to restore Tsuga sieboldii forest should be afforest and make forest gap because It is mid shade tolerant tree and purity percentage of its seed is 1~2%. It was considered that the composition of group mixture forest constituted Pinus parviflora, Tsuga sieboldii, Taxus cuspidata, Camellia japonica, Machilus thunbergii and Acer okamotoanum, etc. will be able to restore native vegetation, after take the form of forest gap by strong thinning and pruning of Pinus thunbergii forest.
Suvi Kim;Yang-gil Kim;Dayoung Lee;Hye-jin Lee;Kyu-Suk Kang
Journal of Korean Society of Forest Science
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제112권1호
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pp.40-56
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2023
Tree species within the Populus genus grow rapidly and have an excellent capacity to absorb carbon, conferring substantial ability to effective purify the environment. Poplar breeding can be achieved rapidly and efficiently if a genetic linkage map is constructed and quantitative trait loci (QTLs) are identified. Here, a high-density genetic linkage map was constructed for the control pollinated progeny using the genotyping-by-sequencing (GBS) technique, which is a next-generation sequencing method. A search was also performed for the genes associated with quantitative traits located in the genetic linkage map by examining the variables of height and diameter at root collar, and resilience to insect damage. The height and diameter at root collar were measured directly, while the ability to recover from insect damage was scored in a 4-year-old breeding population of aspen hybrids (Odae19 × Bonghyeon4 F1) established in the research forest of Seoul National University. After DNA extraction, paternity was confirmed using five microsatellite markers, and only the individuals for which paternity was confirmed were used for the analysis. The DNA was cut using restriction enzymes and the obtained DNA fragments were prepared using a GBS library and sequenced. The analyzed results were sorted using Populus trichocarpa as a reference genome. Overall, 58,040 aligned single-nucleotide polymorphism (SNP) markers were identified, 17,755 of which were used for mapping genetic linkages. The genetic linkage map was divided into 19 linkage groups, with a total length of 2,129.54 cM. The analysis failed to identify any growth-related QTLs, but a gene assumed to be related to recovery from insect damage was identified on linkage group (chromosome) 4 through genome-wide association study.
Environment-friendly harvesting is practiced to maintain ecosystem, landscape, and forest protection functions. The present study was conducted at Simgok-ri, Sinbuk-myeon, Pocheon, Gyeonngi-do, where a 41-50-year-old Japanese larch forest was harvested in an environment-friendly manner from 2017 to 2019. The dynamics of organic matter in this forest were investigated at three years after the harvest. Specifically, organic matter content was measured on the forest floor and in overstory biomass, litterfall, and soil up to 30 cm in depth from June 2020 to January 2021. Owing to the harvest, the amount of overstory biomass of the Japanese larch stands decreased from 142.22 to 44.20 t ha-1. On the forest floor, the amount of organic matter was 32.87 t ha-1 in the control plots and 23.34 t ha-1 in the harvest plots. Annual litterfall was 4.43 t ha-1 yr-1 in the control plots and 1.16 t ha-1 yr-1 in the harvest plots. Soil bulk density in the B horizon was 0.97 g cm-3 in the control plots and 1.06 g cm-3 i n the harvest plots. Soil organic matter content was 11.5% in the control plots and 12.8% in the harvest plots. The total amount of soil organic matter did not differ significantly between the control plots (245.21 t ha-1) and harvest plots (263.92 t ha-1), although the amount of soil organic matter tended to be higher in the harvest plots. The total amount of organic matter in the forest was estimated to be 406.48 t ha-1 in the control plots and 338.21 t ha-1 in the harvest plots. In the harvest plots, the ratio of aboveground organic matter decreased to 13.1% and soil organic matter increased to 78.0%, indicating that the distribution of organic matter changed significantly in these plots. Overall, the carbon accumulated in aboveground biomass was substantially reduced by environment-friendly harvesting, whereas the soil carbon level increased, which played a role in mitigating the reduction of system carbon in the forest. These results highlight one possible resolution for forest management in terms of coping with climate change. However, given that only three years of environment-friendly harvesting data were analyzed, further research on the dynamics of organic matter and tree growth is needed.
Jihee Seo;Sukyung Kim;Hyun Seok Kim;Junghwa Chun;Myoungsoo Won;Keunchang Jang
Korean Journal of Agricultural and Forest Meteorology
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제25권4호
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pp.427-435
/
2023
Changes in flowering time due to weather fluctuations impact plant growth and ecosystem dynamics. Accurate prediction of flowering timing is crucial for effective forest ecosystem management. This study uses a process-based model to predict flowering timing in 2023 for five major tree species in Korean forests. Models are developed based on nine years (2009-2017) of flowering data for Abeliophyllum distichum, Robinia pseudoacacia, Rhododendron schlippenbachii, Rhododendron yedoense f. poukhanense, and Sorbus commixta, distributed across 28 regions in the country, including mountains. Weather data from the Automatic Mountain Meteorology Observation System (AMOS) and the Korea Meteorological Administration (KMA) are utilized as inputs for the models. The Single Triangle Degree Days (STDD) and Growing Degree Days (GDD) models, known for their superior performance, are employed to predict flowering dates. Daily temperature readings at a 1 km spatial resolution are obtained by merging AMOS and KMA data. To improve prediction accuracy nationwide, random forest machine learning is used to generate region-specific correction coefficients. Applying these coefficients results in minimal prediction errors, particularly for Abeliophyllum distichum, Robinia pseudoacacia, and Rhododendron schlippenbachii, with root mean square errors (RMSEs) of 1.2, 0.6, and 1.2 days, respectively. Model performance is evaluated using ten random sampling tests per species, selecting the model with the highest R2. The models with applied correction coefficients achieve R2 values ranging from 0.07 to 0.7, except for Sorbus commixta, and exhibit a final explanatory power of 0.75-0.9. This study provides valuable insights into seasonal changes in plant phenology, aiding in identifying honey harvesting seasons affected by abnormal weather conditions, such as those of Robinia pseudoacacia. Detailed information on flowering timing for various plant species and regions enhances understanding of the climate-plant phenology relationship.
Cholho Song;Hyun-Ah Choi;Jiwon Son;Youngjin Ko;Stephan A. Pietsch;Woo-Kyun Lee
Korean Journal of Environmental Biology
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제41권4호
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pp.400-412
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2023
In this study, the biogeochemistry management (BGC-MAN) model was applied to North and South Korea pine and oak forest stands to evaluate the Net Primary Productivity (NPP), an indicator of forest ecosystem productivity. For meteorological information, historical records and East Asian climate scenario data of Shared Socioeconomic Pathways (SSPs) were used. For vegetation information, pine (Pinus densiflora) and oak(Quercus spp.) forest stands were selected at the Gwangneung and Seolmacheon in South Korea and Sariwon, Sohung, Haeju, Jongju, and Wonsan, which are known to have tree nurseries in North Korea. Among the biophysical information, we used the elevation model for topographic data such as longitude, altitude, and slope direction, and the global soil database for soil data. For management factors, we considered the destruction of forests in North and South Korea due to the Korean War in 1950 and the subsequent reforestation process. The overall mean value of simulated NPP from 1991 to 2100 was 5.17 Mg C ha-1, with a range of 3.30-8.19 Mg C ha-1. In addition, increased variability in climate scenarios resulted in variations in forest productivity, with a notable decline in the growth of pine forests. The applicability of the BGC-MAN model to the Korean Peninsula was examined at a time when the ecosystem process-based models were becoming increasingly important due to climate change. In this study, the data on the effects of climate change disturbances on forest ecosystems that was analyzed was limited; therefore, future modeling methods should be improved to simulate more precise ecosystem changes across the Korean Peninsula through process-based models.
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