• Title/Summary/Keyword: Treatment Efficacy

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Apoptotic Activity of Curcumin and EF-24 in HTB-41 Human Salivary Gland Epidermoid Carcinoma Cells

  • Kim, Ji-Won;Lee, Seul Ah;Go, Dae-San;Park, Byung-Sun;Kim, Su-Gwan;Yu, Sun-Kyoung;Oh, Ji-Su;Kim, Chun Sung;Kim, Jeongsun;Park, Jong-Tae;Kim, Do Kyung
    • International Journal of Oral Biology
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    • v.40 no.2
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    • pp.63-69
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    • 2015
  • Curcumin (diferuloylmethane), a constituent of turmeric powder derived from the rhizome of Curcuma longa, has been shown to inhibit the growth of various types of cancer cells by regulating cell proliferation and apoptosis. However, a need exists to design more effective analogs because of curcumin's poor intestinal absorption. EF-24 (diphenyl difluoroketone), the monoketone analog of curcumin, has shown good efficacy in anticancer screens. However, the effects of curcumin and EF-24 on salivary gland epidermoid carcinoma cells are not clearly established. The main goal of this study was to investigate the effects of curcumin and EF-24 on cell growth and induction of apoptosis in human salivary gland epidermoid carcinoma cells. Our studies showed that curcumin and EF-24 inhibited the growth of HTB-41 cells in a dose- and time-dependent manner, and the potency of EF-24 was > 34-fold that of curcumin. Treatment with curcumin or EF-24 resulted in nuclear condensation and fragmentation in HTB-41 cells, whereas the control HTB-41 cell nuclei retained their normal regular and oval shape. Curcumin and EF-24 promoted proteolytic cleavages of procaspase-3/-7/-9, resulting in an increase in the amount of cleaved caspase-3/-7/-9 in the HTB-41 cells. Caspase-3 and -7 activities were detected in viable HTB-41 cells treated with curcumin or EF-24. These results suggest that the curcumin and EF-24 inhibit cell proliferation and induce apoptosis in HTB-41 human salivary gland epidermoid carcinoma cells, and that they may have potential properties as an anti-cancer drug therapy.

A Typology of Maritally Violent Men: Testing the Three Batterer Subtypes Derived from Theory (가정폭력 행위자의 유형: 이론에 따른 세 하위유형의 검증)

  • Chang, Hee-Suk;Kim, Ye-Sung
    • Korean Journal of Social Welfare
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    • v.56 no.3
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    • pp.303-325
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    • 2004
  • This study investigated subtypes of men who batter, and explored the differences among them. It was based on 217 subjects from all around the nation who received legal punishment. In the analysis of the typology, we specifically tested whether the Holtzworth-Munroe and Stuart proposed typology was verified. The results of the cluster analysis revealed support for their theoretical distinction for three types of abusers. These results imply that Holtzworth-Munroe and Stuart's batterer typology is applicable to Korean batterers to some degree. Type 1 men demonstrated the lowest levels of physical and psychological abuse toward their wives and were the least likely to have had a history of child abuse or alcohol problems. These men had lower MCMI scores and did not show any extraordinary personality traits. Men in this category were violent only against their wives, had relatively liberal sex role attitudes and had the most satisfaction in their intimate relationships. Type 2 men were violent only at home, using a moderate level of violence. These men had very high levels of dependency on others and showed a borderline, avoident or passive-aggressive personality. The amount of alcohol consumption was similar to Type 1, but scores of jealousy, self-esteem, and attitudinal variables were similar to Type 3 men. They lacked assertiveness skills and reported the least marital satisfaction. Type 3 men used the most severe violence and were violent both inside and outside the home. These men showed signs of antisocial and aggressive personality. They had experienced frequent physical abuse during childhood, were the most likely to abuse alcohol and had lower self-control. Type 3 were the most traditional in their views of women's roles and had attitudes supporting violence. Based upon the study findings, practical implications of enhancing treatment efficacy were considered.

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EFFECT OF THE CHLORHEXIDINE AND FLUORIDE-CONTAINING VARNISH ON THE LEVEL OF SALIVARY MUTANS STREPTOCOCCI (Chlorhexidine과 fluoride 성분의 varnish가 타액 내 mutans streptococci 수에 미치는 효과)

  • Seo, Jeong-Ah;Baik, Byeong-Ju;Yang, Yeon-Mi;Lee, Yong-Hee;Kim, Sang-Hoon;Kim, Jae-Gon
    • Journal of the korean academy of Pediatric Dentistry
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    • v.31 no.4
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    • pp.579-586
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    • 2004
  • The objective of the present prospective trial was to compare the efficacy of chlorhexidine(Cervitec), Fluoride (Fluorprotector) and combination of chlorhexdine and fluoride varnishes in decreasing the level of salivary mutans streptococci. Forty healthy students of school of dentistry, Chonbuk national university were investigated to evaluate mutans streptococci(MS) counts and randomized into four groups to treat with the experimental varnishes; A) untreated group(n=10), B) chlorhexidine group(n=10), C) fluoride group(n=10), D) chlorhexidine and fluoride group(n=10). Dentocult $SM^{(R)}$ (Orion Diagnostica) strip method was used for measurement of the level of mutans streptococci in saliva. Stimulated saliva were collected at baseline for mutans streptococci counts evaluation (ms1), 12 weeks later the completion of each varnish treatment, mutans streptococci counts were re-evaluated. In varnish group with chlorhexidine, fluoride and combination of chlorhexidine and fluoride, the level of mutans streptococci was lower after 12 weeks than at baseline, but there were no significant differences in saliva(p>0.05), when compared with baseline. After 12 weeks, a remarkable reduction was still found in the subjects with high level of mutans streptococci at baseline, but not different in the low and moderate level of mutans streptococci(p>0.05).

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Shelf-life Prediction of ${\gamma}-Irradiated$ Boiled-Dried Anchovies (감마선 조사 건멸치의 저장수명 예측)

  • Kwon, Joong-Ho;Byun, Myung-Woo;Suh, Jae-Soo
    • Korean Journal of Food Science and Technology
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    • v.31 no.6
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    • pp.1557-1562
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    • 1999
  • As a series of studies on the preservation methods for boiled-dried anchovies, determination of sorption properties and shelf-life prediction were made for the samples. Dried anchovies, which were gamma-irradiated at pre-established dose (5 kGy) after packaging in both a polyethylene film (PE, 0.1 mm) and a laminated film $(nylon\;15\;{\mu}m/polyethylene\;100\;{\mu}m,\;NY/PE)$, were subjected to a quality evaluation during 4 months at different storage conditions, such as $15^{\circ}C/68%\;RH,\;25{\circ}C/75%\;RH,\;and\;35^{\circ}C/84%$ RH. The sample showed 5.47% of BET monomolecular layer moisture content and the corresponding water activity, 0.15. The velocity constants of browning reaction and organoleptic changes in the sample were in proportion to storage temperature, and $Q_{10}$, values were ranged from 2.17 to 2.40 in a given packaging and irradiation conditions. In the shelf-life prediction of the stored sample at $25^{\circ}C$, non-irradiated groups packaged in PE and NY/PE were 84 days and 125 days. While 5 kGy-irradiated groups in the same packaging were 126 days and 138 days, respectively. This finding proved the efficacy of laminated-film packaging and irradiation treatment in preserving the quality of dried anchovies.

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The comparison of Effectiveness between Bee Venom and Sweet Bee Venom Therapy on Chronic Lower Back Pain. (만성요통환자에 대한 Bee Venom과 Sweet Bee Venom의 치료효능 비교 연구)

  • Kim, Jae-Hong;Jang, Sun-Hee;Yoon, Hyun-Min;Jang, Kyung-Jun;Ahn, Chang-Beohm;Kim, Cheol-Hong;Song, Choon-Ho;Choi, Han-Na
    • Journal of Pharmacopuncture
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    • v.11 no.4
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    • pp.15-24
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    • 2008
  • Objective The aim of this study is to investigate if Sweet Bee Venom(SBV) Therapy has the equal effect in comparison with Bee Venom (BV) Therapy on Chronic Lower Back Pain. Methods Clinical studies were conducted to 39 patients who were treated Chronic Lower Back Pain in Dept. of Acupuncture and Moxibustion, Dongeui University from March 1 to June 30, 2008. Subjects were randomly devided into 2 groups : BV treated group(Group A, n=19), SBV treated group(Group B, n=20) In BV treated group(Group A), we treated patients with dry needle acupuncture and BV Therapy. In SBV treated group(Group B), we treated patients with dry needle acupuncture and SBV Therapy. All process of treatment were performed by double blinding method. 1. To estimate the efficacy of venom in controlling pain, we have checked Visual Analog Scale(VAS). 2. For evaluating functional changes of patients, we have checked Oswestry Lower Back Disability Questionnaire(ODI). 3. To estimate Itching which is the most prominent symptom of allergic reaction, we have checked Visual Analog Scale(VAS). Results 1. In controlling pain, the results of BV treated group(Group A) is more effective than that of SBV treated group(Group B). 2. In promoting function, the results of BV treated group (Group A) is more effective than that of SBV treated group(Group B). 3. In controlling itching, the results of SBV treated group(Group B) is more effective than that of BV treated group(Group A). Conclusions According to the study, SBV Therapy shows more effective result than BV Therapy in controlling itching. But BV Therapy is more effective than SBV Therapy in controlling pain and promoting function.

Insecticidal Activity of Essential Oils against Whitegrub (식물정유의 굼벵이에 대한 살충활성)

  • Lee, Dong Gon;Jung, Young Hak;Choi, Dae Hong;Choi, Sung Hwan;Choo, Ho Yul;Lee, Dong Woon
    • Weed & Turfgrass Science
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    • v.4 no.2
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    • pp.129-134
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    • 2015
  • White grub damages various crops, trees and they can also be one of the most serious pests of turf grass in golf courses. This study was conducted to determine the biocontrol of white grubs with 18 different kinds (anise, camphor, castor, cinnamon, clove oil, citronella, fennel, geranium, lavender, lemongrass, linseed, neem, peppermint, pine, rosemary, tea-tree, thyme, and turpentine) of plant essential oils in laboratory, green house and field. Anise oil (88.9%), linseed oil (100%), and tea-tree oil (88.9%) were highly effective among tested essential oils against 3rd instar of Bifurcanmala aulax in cap vial experiment. However, anise and linseed oils showed low mortality against $3^{rd}$ instar of Popillia japonica in pot greenhouse experiment. Efficacy of anise, linseed oil, and tea-tree was also different depending on target white grub in field trials. Correlated mortality showed 32.6% only in tea-tree oil treatment against pupae of Adoretus tenuimaculatus however, correlated mortality of anise, linseed and tea-tree oil were 54.8, 51.6 and 56.5% respectively against $3^{rd}$ instar of Exomala orientalis in the field trial in Adelscott Country Club in Hapcheon, Gyeongnam province.

Effects of fermented Taraxacum mongolicum by Dongchimi on protection of liver cell and immune activity (포공영(민들레)의 동치미 발효에 의한 간세포 보호 및 면역증진 효과)

  • Choi, Bog-Soo;Kim, Hye-Ja
    • Journal of Society of Preventive Korean Medicine
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    • v.18 no.1
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    • pp.11-21
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    • 2014
  • Objective : This study set out to combine the treatment efficacy of Taraxacum with Dongchimi fermentation and investigate Taraxacum's effects on protection of liver cell and controlling nitric oxide(NO) through experiments, thus checking whether it had values as a physiological active matter. The experimental materials include Taraxacum Dongchimi (TD) and Taraxacum fermented by Dongchimi (TDF). As for methodology, experiments were carried out to compare TD and TDF in components, protection effects for liver cells, anticancer effects on liver cells, and protection effects for brain cells in the aspects of liver function and immunity enhancement. Method : The experimental materials include Taraxacum Dongchimi (TD) and Taraxacum fermented by Dongchimi (TDF). As for methodology, experiments were carried out to compare TD and TDF in components, protection effects for liver cells, anti-cancer effects on liver cells, and protection effects for brain cells in the aspects of liver function and immunity enhancement. Results : As shown in the chromatogram results, each valid component content increased in Taraxacum fermented by Dongchimi (TDF) for each time section. Of them, the valid component content at 36.80 minutes was approximately 2.7 times higher in TDF at 21.8% than in Taraxacum Dongchimi (TD) at 8.28%. TDF generated more excellent protection effects against the toxicity that caused oxidative damage to the liver cell(HepG2) with t-BHP than TD. The survival rate was low in TD of $100{\mu}g/m{\ell}$ and $300{\mu}g/m{\ell}$ and increased to 23.3% in TDF of $100{\mu}g/m{\ell}$. The survival rate was the highest at $300{\mu}M$ with a significant difference of 68.1%(P<0.05). Both TD and TDF showed effects of controlling nitric oxide production according to concentration with TDF recording a higher rate of controlling nitric oxide production than TD. There were significant differences(P<0.05) in the effects of controlling nitric oxide production at 200 ug/ml, 400 ug/ml in both groups. Especially the result TDF of $400{\mu}g/m{\ell}$ was thus similar to those of butein, the positive control group. Conclusion : The result of this studies is that Taraxacum fermented by Dongchimi (TDF) increased the valid component content compared with the simple mixture(TD). The findings clearly show that it is a material with the effects of improving immunity and liver cell protection. If fermentation methods are further developed to use it as a functional material, it will be subject to more opportunities of being used in other functional foods and make a contribution to integrated medicinal food development.

Patient Controlled Analgesia of Alfentanil after a Total Abdominal Hysterectomy: A Comparison of the Intravenous and Epidural Route (전자궁 적출술 후 자가통증조절장치를 이용하여 정맥과 경막외로 투여된 Alfentanil의 진통효과 비교)

  • Choi, Soo Kyeong;Yoon, Seok Hwa;Lee, Jun Hwa;Hwang, Jae Ha;Jung, Woo Suk;Kim, Yoon Hee;Lee, Won Hyung
    • The Korean Journal of Pain
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    • v.20 no.2
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    • pp.169-173
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    • 2007
  • Background: Although the use of intravenous patient controlled analgesia (IVPCA) has been compared to the use of patient conrolled epidural analgesia (PCEA), there is no optimal administration route of alfentanil for the treatment of postoperative pain. This randomized double-blind study compared the efficacy of the use of IVPCA and PCEA for postoperative pain and the side effects after a total abdominal hysterectomy (TAH). Methods: Sixty patients undergoing a TAH were randomly assigned to receive either IVPCA (Group I) or PCEA (Group E) for the infusion of alfentanil for postoperative pain control. In both groups, a loading dose of $750{\mu}g$ alfentanil was administered. All patients received the same continuous infusion rate (0.3 mg/h), bolus dose (0.15 mg), and lockout time (15 min). The incidence of side effects, the VAS (visual analog scale) of pain, blood pressure, and heart rate were checked for 20 hours after the loading dose injection. Results: The VAS of pain was not significantly different between the two groups of patients. The onset of the analgesic effect was significantly more rapid in the Group I patients than in the Group E patients. There was no difference in side effects for either group. Conclusions: When considering multiple factors such as the onset of analgesia, technical difficulties or infection after the procedure, IVPCA using alfentanil is more useful than PCEA for postoperative pain control after a TAH.

Effect of 6-Hydroxydopamine (6-OHDA) on the Expression of Testicular Steroidogenic Genes in Adult Rats

  • Heo, Hyun-Jin;Ahn, Ryun-Sup;Lee, Sung-Ho
    • Development and Reproduction
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    • v.14 no.3
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    • pp.199-205
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    • 2010
  • A neurotoxin, 6-hydroxydopamine (6-OHDA) has been widely used to create animal model for Parkinson's disease (PD). The present study was undertaken to examine whether depletion of brain dopamine (DA) stores with 6-OHDA can make alteration in the activities of the testicular steroidogenesis in adult rats. Young adult male rats (3 months old) were received a single dose of 6-OHDA (200 ${\mu}g$ in 10 ${\mu}{\ell}$/animal) by intracerebroventricular (icv) injection, and sacrificed after two weeks. The mRNA levels of steroidogenesis-related enzymes were measured by qRT-PCRs. Serum testosterone levels were measured by radioimmunoassay. Single icv infusion of 6-OHDA significantly decreased the mRNA levels of CYP11A1 (control:6-OHDA group=$1:0.68{\pm}0.14$ AU, p<0.05), CYP17 (control:6-OHDA group=$1:0.72{\pm}0.13$ AU, p<0.05). There were no changes in the mRNA levels of $3{\beta}$-HSD (control:6-OHDA group=$1:0.84{\pm}0.08$ AU) and $17{\beta}$-HSD (control: 6-OHDA group=$1:0.63{\pm}0.20$ AU), though the levels tended to be decreased in the 6-OHDA treated group. Administration of 6-OHDA decreased significantly the mRNA level of StAR when compared to the level of saline-injected control animals (control:6-OHDA group=$1:0.72{\pm}0.08$ AU, p<0.05). Treatment with single dose of 6-OHDA remarkably lowered serum testosterone levels compared to the levels of control group (control:6-OHDA group=$0.72{\pm}0.24:0.13{\pm}0.03ng/m{\ell}$, p<0.05). Taken together with our previous study, the present study demonstrated that the activities of hypothalamus-pituitary-testis hormonal axis could be negatively affected by blockade of brain DA biosynthesis, and suggested the reduced reproductive potential might be resulted in the animals. More precise information on the testicular steroidogenic activities in PD patients and PD-like animals should be required prior to the generalization of the sex steroid hormone therapy to meet the highest standards for safety and efficacy.

Effects of Total Mixed Rations on Growth Performance and Carcass Charcteristics of Hanwoo Steers (섬유질배합사료 급여가 거세한우의 발육 및 도체특성에 미치는 영향 미치는 영향)

  • Cho, Young Moo;Kwon, Eung Gi;Chang, Sun Sik;Kim, Tae Il;Park, Byung Ki;Kang, Su Won;Paek, Bong Hyun
    • Journal of Animal Science and Technology
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    • v.50 no.3
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    • pp.363-372
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    • 2008
  • To investigate the effects of total mixed ration(TMR) on growth performance and carcass characteristics of Hanwoo steers, sixty steers were randomly assigned to one of four treatment groups; separate feeding of concentrate and rice straw(control), wet TMR(TMRⅠ), dry TMR with 20%(TMRⅡ) and 40%(TMRⅢ) barley bran from 6 to 28 month of age. In growth performance, average daily gains of TMRⅡ group was the highest(0.70kg/day) but significant differences between treatments were not found(p>0.05). Average feed required for kg body weight gain of control group was 11.63kg. Those of TMRⅠ through TMRⅢ were 9.29, 10.46 and 10.71kg, respectively. Dressing percentage and retailed cut percentage were 59% and 63%, without any significant differences between treatments(p>0.05). There were no significant differences in rib-eye area, back fat thickness, meat color and fat color (p>0.05). Average marbling score was higher(p<0.05) in TMRⅠ than in control group.