Water temperature-dependent fluctuations of biochemical and molecular activities in the harmful dinoflagellate, Cochlodinium polykrikoides were studied. In terms of genomic DNA concentration, a similar value of 0.6 was observed at $12^{\circ}C$ and $15^{\circ}C$. However, DNA significantly increased beyond $18^{\circ}C$ (p<0.05), to a maximum of 1.8 at $24^{\circ}C$. DNA concentration significantly decreased to 0.6. The concentrations of RNA and total protein were likely at their highest values of 1.7 and 0.07 ${\mu}g$$ml^{-1}$ at $24^{\circ}C$, respectively. RNA and total protein concentrations began to increase at $15^{\circ}C$. Oxygen availability between lower and higher temperatures was significantly different and increased from $18^{\circ}C$ according to light intensity, regardless of wavelengths (p<0.05). At $24^{\circ}C$, the highest value of the maximum electron transport rate ($ETR_{max}$), ranging from 537.9 (Ch 1) to 602.5 ${\mu}mol$ electrons $g^{-1}$ Chl ${\alpha}s^{-1}$ (Ch 4), was also apparent. Nitrate reductase (NR) and ATPase activities were at their highest values of 0.11 ${\mu}mol$$NO_{2}^{-}$${\mu}g^{-1}$ Chl ${\alpha}h^{-1}$ and 0.78 pmol 100 $mg^{-1}$ at $24^{\circ}C$, respectively. In an analysis of CHN, the concentration of C and N also significantly increased (p<0.05). Most of the measurements for the cellular activities at $27^{\circ}C$, however, were less than at $24^{\circ}C$. These results suggest that the sub-cellular activities of C. polykrikoides are sensitive to changes in water temperature. It may be desirable to estimate at $18^{\circ}C$ the initiation of the massive blooming development of C. polykrikoides. In nature, it will be very difficult to maintain the massive blooms beyond $24^{\circ}C$ because of a possibly significant decrease in molecular activity of C. polykrikoides.
Park, Seongwon;Lee, Hye Won;Lee, Yong Seok;Park, Seok Soon
Korean Journal of Ecology and Environment
/
v.46
no.4
/
pp.488-498
/
2013
A three-dimensional hydrodynamic model was applied to the Lake Euiam. The lake has three inflows, of which Gongji Stream has the smallest flow rate and poorest water. The dam-storage volume, watershed area, lake shape and discharge type of the Chuncheon Dam and the Soyang Dam are different. Therefore, it is difficult to analyze the water plume and mixing pattern due to the difference of the two dams regarding the amount of outflow and water temperature. In this study, we analyzed the effects of different characteristics on temperature and conductivity using the model appropriate for the Lake Euiam. We selected an integrated system supporting 3-D time varying modeling (GEMSS) to represent large temporal and spatial variations in hydrodynamics and transport of the Lake Euiam. The model represents the water temperature and hydrodynamics in the lake reasonably well. We examined residence time and spreading patterns of the incoming flows in the lake based on the results of the validated model. The results of the water temperature and conductivity distribution indicated that characteristics of upstream dams greatly influence Lake Euiam. In this study, the three-dimensional time variable water quality model successfully simulated the temporal and spatial variations of the hydrodynamics in the Lake Euiam. The model may be used for efficient water quality management.
We have investigated geographical distribution and physico-chemical properties of water masses or water types at mid-bottom depth in the neighbouring sea of Cheju Island in August 1986. In 50m layer the Yellow Sea Bottom Cold Water(YSBCW) below $12^{\circ}C$ was observed in the northwestern area of Cheju Island, while the Tsushima Warm Water(TWW) with relatively high temperature$(>16^{\circ}C)$ and salinity more than 34.0 in its southeastern area extended as far as the coast of about 15km. Also, 50m layer at the outside stations of its southwestern area indicated relatively cold water temperature$(11-30^{\circ}C)$, probably due to southward transport of the Yellow Sea Bottom Cold Water(YSBCW . The Yellow Sea Warm Water(YSWW), the mixed water of the YSBCW and the TWW, ranged $13^{\circ}C$ to $16^{\circ}C$ in water temperature and was appeared mainly in the coastal and intermediate area of Cheju Island. And the relatively cold water in the southwestern area and the Tsushima Warm Water were more extensively distributed in 50m layer than the deeper layer. Horizontal distributions of nitrate and phosphate showed a pattern similar to that of water temperature. As it were, the Yellow Sea Bottom Cold Water had the highest concentration of nutrients, while southwestern outside stations had the lowest nutrient contents. Especially, the concentration of nitrate in the latter was remarkably low compared with the value at the other stations. It may be attributed to intensive vertical mixing by collision of the northward driven Tn with the southward driven YSBCW. Also, it was particular that the Tsushima Warm Water indicated relatively high silicate content corresponding to that of the Yellow Sea Bottom Cold Water. Based on the data of $\Delta Si/\Delta P$ ratio, it seems that the mid-bottom waters in this study area are younger than the surface or intermediate water in the Korean East Sea.
In this study, we evaluated the changes of fruit quality indices during fruit development and ripening in Korean new pear cultivar 'Changjo', developed from a cross between 'Tama' and '81-1-27' ('Danbae' ${\times}$ 'Okusankichi') in 1995 and named in 2009, to determine appropriate harvest time and to enhance the market quality and broaden the cultivation area. The fruits of 'Changjo' pears harvested from 132 days after full bloom (DAFB) to 160 DAFB. Fruit growth and quality indices were monitored at 1 week interval by measuring fruit weight, length, diameter, firmness, and taste related quality indices. The calculated fruit fresh weight increased continuously with fruit development and reached to an average of 594g on Sep. 20 (160 DAFB). The ratio of length to diameter declines as fruit maturation progress, resulting in 0.898 for ripe fruit stage as a round oblate shape. Flesh firmness of 'Changjo' pears showed over 30N until 153 DAFB and then decreased abruptly with fruit ripening, reaching a final level of about 26.44N on 160 DAFB. Starch content of fruit sap was also decreased abruptly after 146 DAFB which decreased almost half of the fruits harvested at 139 DAFB. In parallel with the decrease of flesh firmness, ethanol insoluble solids (EIS) content decreased sharply with fruit ripens, only 50% of EIS was detected on the fruits harvested on 160 DAFB when compared to that of the fruits harvested on 139 DAFB (Aug. 30). The maximum value of soluble solids contents was observed in the fruits harvested on 153 DAFB, resulting in $14.2^{\circ}Brix$. The changes of skin color difference $a^*$ which means loss of green color occurred only after 139 DAFB, coincide with the decrease of SPAD value of the fruit skin. The sugars of the 80% ethanol soluble fraction consisted mainly of fructose, sorbitol, glucose and sucrose, also increased during maturation and ripening. Fructose and sucrose contents were larger than those of glucose and sorbitol in flesh tissues. These results were explained that stored starch is converted to soluble sugars during fruit maturation, mainly in fructose and sucrose increasing the sweetness of this cultivar. Total polyphenols were increased up to middle of fruit maturation (146 DAFB) and then decreased continuously until the end of fruit maturation. Consequently, our results suggested that the commercial harvest time of 'Changjo' pears should not be passed 153 DAFB and late harvest of this cultivar would not good for quality maintenance during shelf-life. As a result of the post-harvest low-temperature acclimation experiment during the short-term transportation period, fruits harvested at 146 DAFB tended to maintain higher firmness after 14 days of simulated marketing at $25^{\circ}C$ compared to fruits harvested at 153 DAFB regardless of temperature set. And, the slower the rate of decrease to the final transport temperature of $5^{\circ}C$, the higher the incidence of internal browning and ethylene production. Therefore, in order to suppress the physiological disorder and to maintain the fruit quality when exporting to Southeast Asia in the 'Chanjo' pears, it is desirable to lower the temperature of the fruits within a short time after harvest and to set the harvest time before 146 days after full bloom.
Phenomenon of vegetation recruitment on the sand bar is drastically rising in the streams and rivers in Korea. In the 1960s prior to industrialization and urbanization, most of the streams were consisted of sands and gravels, what we call, 'White River'. Owing to dam construction, stream maintenance, etc. carried out since the '70s, the characteristic of flow duration and sediment transport have been disturbed resulting in the abundance of vegetation in the waterfront, that is, 'Green River' is under progress. This study purposed to identify the correlation among water level, water temperature, rainfall, soil moisture and soil texture out of the factors which give an effect on the vegetation recruitment on the sand bar of unregulated stream. To this purpose, this study selected the downstream of Naeseong Stream, one of sand rivers in Korea, as the river section for test and conducted the monitoring and analysis for 289 days. In addition, this study analyzed the aerial photos taken from 1970 to 2009 in order to identify the aged change in vegetation from the past to the present. The range of the tested river section was 361 m in transverse length and about 2 km in longitudinal length. According to the survey analysis, the tested river section in Naeseong Stream was a gaining river showing the higher underground-water level by 20~30 m compared to Stream water level. The difference in the underground water temperature was less than $5^{\circ}C$ by day and season and the Stream temperature did not fall to $10^{\circ}C$ and less from May when the vegetation germination begins in earnest. The impact factor on soil moisture was the underground water level in the lower layer and the rainfall in the upper layer and it was found that all the upper and lower layer were influenced by soil particle size. The soil from surface to 1 m-underground out of 6 soil moisture-measured points was sand with the $D_{50}$ size of 0.07~1.37 mm and it's assumed that the capillary height possible in the particle size would reach around 14~43 cm. On the other hand, according to the result of space analysis on the tested river section of unregulated stream for 40 years, it was found that the artificial disturbance and drought promoted the vegetation recruitment and the flooding resulted in the frequency extinction of vegetation communities. Even though the small and large scales of recruitment and extinction in vegetation have been repeated since 1970, the present vegetation area increased clearly compared to the past. It's found that the vegetation area is gradually increasing over time.
One of the critical problems to preserve books and documents in libraries and archives is the deterioration. Some of previous results showed that the major cause of paper deterioration was the acid-catalyzed hydrolysis of the cellulose in paper fibres and aging rate of acidic paper was faster than that of alkaline paper. Therefore, It is necessary to remove the acid in the paper for reducing the rate of paper deterioration. It has been reported to extend the useful life of acidic paper by three to five times. Recently, It has been recognized the need for an effective method of deacidifying large quantities of books and document. However, in the previous many reports little attention was paid to the effect of paper additives. In this paper, We carried out experiment about the effect of additives on paper aging and the effect of deacidification by the gaseous ethanolamines (monoehtanolamine, diethanolamine, triehtanolamine). In result, it was found that the strength of aging was in the order of the alum+rosin>alum >AKD> control and the rate of deacidification was in the order of the monoethanolamine>diethanolamine>triethanolamine. The treatment with the gaseous ethanolamines caused decreasing of brightness and dropping of fold endurances. However, deacidification by combination treatment of the various gaseous ehtnaolamines prevented from decreasing of brightness and dropping of folding endurances.
The blue-light effect on the grown as well as on the physiological activity of some major horticultural plants in Korea has been investigated. The light quality used for the work was obtained from sunlight filtered by an orangecolored polyethylene film which removed about 70% of visible light in the spectral region of $350㎚{\sim}500㎚$. The film was developed in this laboratory especially for the work and named BCR film meaning blue color-removing film. The light environment in the plastic house which was built with BCR film provided plants with the blue color-deficient sunlight. Thus, the photobiological effect of blue light could be examined conversely by comparing with the effect of white sunlight in a conventional plastic house built with colorless polyethylene film. In a sense of applicability to horticulture, two remarkable effects of the blue color-deficient sunlight on plant physiology were observed: First, it enhanced to a great extent the growth activity of plants-pepper, cucumber, zucchini, tomato, and leaf lettuce at the vegetative stage as well as at the reproductive stage, as demonstrated by their yield which were in average $40{\sim}50%$ increased compared with the control (under white sunlight). Second, it improved significantly the cold tolerance of plants, as exhibited with their resistance to chilling during treatment in a cold chamber maintained at a temperature which caused chilling injury to the plants of control. The visualized effects were reflected on the physiological activity of cells on organelle level. Chloroplast isolated from the plant leaves grown under BCR film showed considerably stronger photosynthetic activity, as judged by the increased electron transport rate of illuminated chloroplast, than that from leaves grown under white PE film. Mitochondria from leaves grown under BCR film maintained normal respiration activity until temperature decreased to a few degree($^{\circ}C$) lower than the temperature which caused respiratory inhibition to mitochondria obtained from leaves of the control.
In the southern sea of Korea and the areas of Tsushima warm currents the relationship between the distributions of eggs and larvae of anchovy (Engraulis japonica) and oceanic conditions was examined on July and August 1997, The south Korean coastal waters, the water temperature of below $20\~23^{\circ}$ and the salinity of above 33.0 (PSU), the mixed waters between the south Korean coastal waters and the Tsushima warm currents, $21\~25^{\circ}$ and $32.0\~32.5$ and the Tsushima warm currents, above $26^{\circ}$ and below 31.5 were distributed at the surface layer. The Tsushima warm currents were distributed at the northeast of Jeju Is. and off the southern sea of Korea. As an appearance of warm streamer, the mixed waters were intruded into the coastal areas of Komun Is.$\~$Sori Is. and Sori Is.$\~$Yokji Is.. Approximate paths of surface water by the drift card experiments were similar with the intrusions of the warm water identified from the water temperature and salinity distributions. The distributions of chlorophyll concentration were consistent with the distributions of water temperature and salinity, Anchovy eggs and larvae were mostly distributed at Komun Is., Yokji Is, and the southwest of Koie Is. where chlorophyll concentrations were high and cyclonic circulations by the warm water intrusions (warm streamers) were formed.
$Ta_2O_5$ thin film IS a promising material for the high dielectrics of ULSI DRAM. In this study, $Ta_2O_5$ thin film was grown on p-type( 100) Si wafer by thermal metal organic chemical vapo deposition ( MCCVD) method and the effect of operating varialbles including substrate temperature( $T_s$), bubbler temperature( $T_ \sigma$), reactor pressure( P ) was investigated in detail. $Ta_2O_5$ thin film were analyzed by SEM, XRD, XPS, FT-IR, AES, TEM and AFM. In addition, the effect of various anneal methods was examined and compared. Anneal methods were furnace annealing( FA) and rapid thermal annealing( RTA) in $N_{2}$ or $O_{2}$ ambients. Growth rate was evidently classified into two different regimes. : (1) surface reaction rate-limited reglme in the range of $T_s$=300 ~ $400 ^{\circ}C$ and (2: mass transport-limited regime in the range of $T_s$=400 ~ $450^{\circ}C$.It was found that the effective activation energies were 18.46kcal/mol and 1.9kcal/mol, respectively. As the bubbler temperature increases, the growth rate became maximum at $T_ \sigma$=$140^{\circ}C$. With increasing pressure, the growth rate became maximum at P=3torr but the refractive index which is close to the bulk value of 2.1 was obtained in the range of 0.1 ~ 1 torr. Good step coverage of 85. 71% was obtained at $T_s$=$400 ^{\circ}C$ and sticking coefficient was 0.06 by comparison with Monte Carlo simulation result. From the results of AES, FT-IR and E M , the degree of SiO, formation at the interface between Si and TazO, was larger in the order of FA-$O_{2}$ > RTA-$O_{2}$, FA-$N_{2}$ > RTA-$N_{2}$. However, the $N_{2}$ ambient annealing resulted in more severe Weficiency in the $Ta_2O_5$ thin film than the TEX>$O_{2}$ ambient.
Upper-air synoptic data and surface weather elements such as temperature, relative humidity, wind speed, cloud and precipitation were analyzed in some detail to determine the characteristics of Nopsae, a foehn-like surface wind over the Youngsuh region of Central Korea. NOAA AVHRR and GMS images are also referenced to identify the distribution of clouds and precipitation to classify the tpyes of foehn over the study area. The data period examined is from 1982 until 1993 of spring and summer months from March through August. Results of the anaylsis are as follows. Warm and dry air penetration over the Younesuh region has experienced on foehn days occured between March 21 and August 10 during study perion. The mean annual number of foehn the days were 28. Foehn phenomena were prominent during March 21-25, April 5-15, May 25-June 10, and June 26-30 pentads. The intensity of the phenomena can be evaluated as the difference of daily maximum temperature and relative humidity between windward sites and leeward sites. The intensity of daily maximum temperature reached 14.5$^{\circ}C$, but most values were in the range of 5.0-7.5$^{\circ}C$ (61%). Although strong intensity of foehns usually develop in June, it is common that farmers in the region experince more aridity during the foehnday of April and May due to the transplantation of rice seedlings. Long-run foehn are not common phenomena and 55% of foehn terminate in one day, but there is a record that Nopsae persisted up to 9 days continuously. The author identified using the cloud and precipitation data out of NOAA-11, AVHRR and GMS images is that one of them has no precipitation over windward side. The available data and the results of the analysis are somewhat inadequate. Since the results imply that wave phenomenon is potentially important in terms of local surface weather and vertical momentum transport, more detailed theoretical and observational studies are necessary to clarify the mechanism and the impacts of Nopsae.
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