• Journal of Plant Biotechnology
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• v.30 no.3
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• pp.233-239
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• 2003

We have developed a reliable and high-frequency genetic transformation and regeneration system via somatic embryogensis of Eleutherococcus sessiliflorus. Embryogenic callus obtained from seed were co- cultivated with Agrobacterium tumefaciens strain EHA101/pIG121Hm harboring genes for intron-$\beta$-glucoronidase(GUS), kanamycin and hygromycin resistance. Following co-cultivation, two types of samples(fine embrogenic calli and early globular embryo clusters) were cultivated on Murashige and Skoog(MS) medium containing 1 mg/L2.4-D for 3day in dark. Transient expression of GUS gene was found to be higher in the early globular embryo clusters than in the embryogenic calli. Also, co-cultivated period affected expression of GUS gene; the best result was obtained when globular embryo clusters were co-cultivated with Agrobacterium for 3 days. Subsequently, this callus transferred to selective MS medium containing 1mg/L2.4-D, 50mg/L kanamycin or/and 30mg/L hygromycin and 300mg/L cefortaxime. These embryogenic calls were subcultured to the same selection medium at every 2 weeks intervals. Approximately 24.5% of the early globular embryos co-cultivated with Agrobacterium for 3days produced kanamycin or/and hygromycin-resistant calli. Transgenic somatic embryos were converted into plantlets in half strength MS medium supplemented with 3mg/L GA$_3$ kanamycin and were confirmed by GUS histochemical assay and polymerase chain reaction analysis. Genomic Southem blot hybridization confirmed the incorporation of NPT II gene into the host genome.

• Advances in pediatric surgery
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• v.12 no.2
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• pp.183-191
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• 2006

Thyroid nodules are less common in children than in adults and their management is still controversial. The clinical presentations, operations, complications, histopathologic findings, and postoperative progressions of 34 pediatric patients that underwent thyroidectomy for palpable thyroid nodule at the Department of Surgery, Seoul National University Children Hospital between 1986 and 2003 were studied retrospectively by reviewing medical records and telephone interviews. The mean age of the patients was 11.8 years old. There were 23 females (67.6 %) and 11 males (32.4 %). Surgical indications were clinical need of histological confirmation (n=15), unresponsiveness to thyroxin replacement therapy (n=10), suggestion of the carcinoma on fine needle aspiration cytology (n=5), cosmetic purpose-a huge benign nodule (n=2), completion thyroidectomy for medullary thyroid carcinoma (n=1), and prophylactic thyroidectomy in a MENIIpatient (n=1). Unilateral Lobectomy was performed in 20 patients (57.1 %), subtotal thyroidectomy in 8 (22.9 %), total thyroidectomy in 5 (14.7 %), and completion thyroidectomy in 1 (2.9 %). Lymph node dissection was performed in 9 cases. Benign tumor was found in 23 patients (67.6 %), adenomatous goiter (n=18) and follicular adenoma (n=5). Malignant tumor was found in 11 children (32.4 %), 9 papillary carcinomas (26.5 %), and 2 medullary carcinomas (5.9 %). Of the 9 papillary carcinomas, 7 cases (77.8 %) had lymph node metastasis. No lymph node metastasis was found in 2 medullary carcinomas. Complications developed in 5 cases - transient hypocalcaemia (n=2), and temporary hoarseness (n=3). There was no mortality. Median follow-up period was 7.4 years (0.5-18 years). One patient showed recurrence in cervical lymph nodes 10 years after surgery and modified radical neck dissection was performed. Because of the high incidence of malignancy and advanced stage at initial presentation, more meticulous diagnostic work up is necessary for children with thyroid nodule, and more radical surgical treatment should be performed when malignant nodule is suggested.

• Advances in pediatric surgery
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• v.20 no.1
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• pp.7-11
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• 2014

The present study compared the postoperative analgesic effects of ilioinguinal and iliohypogastric nerve block with infiltration of local anesthetics (bupivacaine) into the wound in children after inguinal hernia repair. Ninety children below 7 years old who were scheduled elective inguinal hernia repair were randomly allocated into one of three groups. The patients in nerve block (NB) group, ilioinguinal and iliohypogastric nerve block was done with 0.5 mL/kg of 0.25% bupivacaine. The patients in infiltration of local anesthetics (LI) group, 0.5 mL/kg of 0.25% bupivacaine was infiltrated into the wound after surgery. The patients in control group were allocated as a Control group. Postoperative pain was assessed at 1, 3, 5, and 24 hours after operation with FLACC scale and additional analgesic consumption were counted. The three groups were not significantly different in age, sex, body weight, and duration of operation. Pain scores at 1 hour and 3 hours after operation were significantly higher in Control group than in NB group and LI group (p<0.01), whereas there were no difference between NB group and LI group. The rescue analgesics administration was significantly higher in Control group (n=11) than in NB group (n=6) and LI group (n=7) (p<0.05). There were 2 cases of transient femoral nerve palsy in NB group. Both of ilioinguinal and iliohypogastric nerve block and infiltration of local anesthetics into the wound provided effective postoperative analgesia in early postoperative period following inguinal hernia repair in children. But no difference between the two methods. Technically, infiltration of local anesthetics into the wound was easier and safer than ilioinguinal and iliohypogastric nerve block.

• The Journal of the Korea Contents Association
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• v.16 no.1
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• pp.52-59
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• 2016

For almost of concrete structures by placing in cold weather, it is very important that the selection of curing method at early aged construction stage. The Exothermic curing method with hot wire and rapid hardening cement is used mostly to prevent the initial cracks and the strength decrease. Most of the construction sites, however, have not been applied to the optimal curing method caused by the simple approaches and the empirical judgements. Therefore, this paper has proposed a evaluation algorithm of the exothermic curing method for representing heating temperature, period, position of hot wire by analyzing the transient heat transfer solution. This has been implemented, moreover, using an object oriented programming language to develop structural analysis system taking account risk parameters. This system is composed of input module, database module, database store module, analysis module, and result generation module. Linkage interface between the central database and each of the related module is implemented by the visual c# concept. Graphic user interface and the relational database table are supported for user's convenience.

• Archives of Plastic Surgery
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• v.34 no.3
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• pp.342-345
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• 2007

Purpose: A common side effect of the scalp reduction is a creation of a 'slot' with the hair growing in the opposite directions away from the scar. Overcoming the unnatural appearance of the slot has been a vexing problem in the scalp reduction surgery. None of the conventional corrective surgical techniques provides a complete and satisfactory aesthetic result. The Frechet flap is a triple transposition flap used for the correction of the slot defect secondary to scalp reduction surgery, seldom needing further scar revision. The Frechet technique provides a solution to the problem of the central slot concealment that is unattainable by other means, such as; Z-plasty and mini-graft. Methods: Authors applied the Frechet technique to Asian patients who had undergone scalp reduction and operated on 4 patients from March, 2000 to January, 2001. Average follow-up period was 13 months. Patients with long scars passing through the temporoparietoccipital zone were excluded. All the undermining was performed in the subgaleal plane, reaching the upper auricular sulcus and stopping just above the nuchal ridge. Results: None of the patients experienced infection, hematoma, nor any permanent hair loss. Transient telogen effluvium at the distal end of flap 2 and 3 was noticeable in one case. Conclusion: In conclusion, the results are aesthetically satisfactory without any significant complications.

• Korean Journal of Head & Neck Oncology
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• v.14 no.2
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• pp.206-213
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• 1998

Primary hyperparathyroidism is still uncommomn disease in Korea. However the frequency of this disease has been slowly increased with routine measurement of serum calcium and increasing awareness of hyperparathyroidism in recent years. The diagnosis is established by a persistent elevation of serum calcium and parathyroid hormone and by clinical evaluation. This is a report of eleven patients with primary hyperparathyroidism treated with surgical operation during a period from 1983 to 1997 at Department of Hospital. Authors analyzed the cases to evaluate clinical characteristics and outcome of surgical treatment retrospectively. The result was as follows. 1) In sex distribution, female patients were eight and three were male, the age distribution ranged from 18 to 67 years. 2) The presenting clinical manifestations were renal and urinary stone in eight, bone pain or fracture in six, muscle weakness in four, neurologic symptoms in four, neck mass in three, hypertension in two, and G-I symptoms in one. 3) All patients showed hypercalcemia and elevated serum parathyroid hormone level. 4) Preoperative localization study was performed with computerized tomography, ultrasonography, MRI, arteriography and thyroid scaning. 5) The tumor locations were left lower in eight, left upper in one, right lower in one, and right upper location was one case. 6) Histopathologic findings disclosed adenoma in all cases. 7) All patients were treated by surgical excision and postoperatively transient hypocalcemia occurred in six patients, but no other complication was developed.

• Journal of the Korean Society of Safety
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• v.21 no.2 s.74
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• pp.143-149
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• 2006

This paper deals with the space-time finite element analysis of two-dimensional vibration problem with a single variable. The method of space-time finite elements enables the simpler solution than the usual finite element analysis with discretization in space only. We present a discretization technique in which finite element approximations are used in time and space simultaneously for a relatively large time period. The weighted residual process is used to formulate a finite element method for a space-time domain. A stability problem is described and some investigations for chosen type of rectangular space-time finite elements are carried out. Instability is caused by a too large time step of successive time steps in the traditional time-dependent problems. It has been shown that the numerical stability of time-stepping on the larger time steps is quite good. The unstructured space-time finite element not only overcomes the shortcomings of the stability in the traditional numerical methods, but it is also endowed with the features of an effective computational technique. Some numerical examples have been presented to illustrate the efficiency of the described method.

• Nuclear Engineering and Technology
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• v.45 no.5
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• pp.613-624
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• 2013

Multi-dimensional two-phase phenomena occur in many industrial applications, particularly in a nuclear reactor during steady operation or a transient period. Appropriate modeling of complicated behavior induced by a multi-dimensional flow is important for the reactor safety analysis results. SPACE, a safety analysis code for thermal hydraulic systems which is currently being developed, was designed to have the capacity of multi-dimensional two-phase thermo-dynamic phenomena induced in the various phases of a nuclear system. To validate the performance of SPACE, a two-dimensional two-phase flow test was performed with slab geometry of the test section having a scale of $1.43m{\times}1.43m{\times}0.11m$. The test section has three inlet and three outlet nozzles on the bottom and top gap walls, respectively, and two outlet nozzles installed directly on the surface of the slab. Various kinds of two-dimensional air/water flows were simulated by selecting combinations of the inlet and outlet nozzles. In this study, two-dimensional two-phase void fraction profiles were quantified by measuring the local gap impedance at 225 points. The flow conditions cover various flow regimes by controlling the flow rate at the inlet boundary. For each selected inlet and outlet nozzle combination, the water flow rate ranged from 2 to 20 kg/s, and the air flow rate ranged from 2.0 to 20 g/s, which corresponds to 0.4 to 4 m/s and 0.2 to 2.3 m/s of the superficial liquid and gas velocities based on the inlet port area, respectively.

• Journal of Power System Engineering
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• v.12 no.1
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• pp.13-19
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• 2008

In this study, a variable induction and exhaust system is applied to turbocharged diesel engine to improve the volumetric efficiency, especially, in a low and transient engine speed range where much of the pollutant matters are expelled out. The volumetric efficiency is known as one of the most important factor which affects significantly engine performance, fuel economy and further emission and noise level. As the torque increase with the engine speed up, the gas flow in an exhaust pipe become pulsating and then has an effect on boost up capacity of air charging into the cylinder and expelling capacity to atmosphere simultaneously. But at a low and idling speed, the pulsation effect was not so significant. Accordingly, resonator was employed to compensate their loss. The variable induction system consists of the secondary pipe, resonator, intercooler, and torque variance were examined with extended operating conditions. In the mean time, for interpretation and well understanding for the phenomena of wave action that arising during intake and exhaust process between turbocharger and variable intake system, the concept of the combined supercharging was introduced. Some of results are depicted which deal with a pressure history during valve events of induction process. Consequently, by the governing of these phase and amplitude of pulsating wave, it enables us to estimate and evaluate for the intake system performance and also, designing stage of the system layout.

• Journal of the Korean institute of surface engineering
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• v.48 no.6
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• pp.297-302
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• 2015

Nanocrystalline diamond(NCD) coated aluminium plates were prepared and applied as heat sinks for LED modules. NCD films were deposited on 1 mm thick Al plates for times of 2 - 10 h in a microwave plasma chemical vapor deposition reactor. Deposition parameters were the microwave power of 1.2 kW, the working pressure of 90 Torr, the $CH_4/Ar$ gas ratio of 2/200 sccm. In order to enhance diamond nucleation, DC bias voltage of -90 V was applied to the substrate during deposition without external heating. NCD film was identified by X-ray diffraction and Raman spectroscopy. The Al plates with about 300 nm thick NCD film were attached to LED modules and thermal analysis was carried out using Thermal Transient Tester (T3ster) in a still air box. Thermal resistance of the module with NCD/Al plate was 3.88 K/W while that with Al plate was 5.55 K/W. The smaller the thermal resistance, the better the heat emission. From structure function analysis, the differences between junction and ambient temperatures were $12.1^{\circ}C$ for NCD/Al plate and $15.5^{\circ}C$ for Al plate. The hot spot size of infrared images was larger on NCD/Al than Al plate for a given period of LED operation. In conclusion, NCD coated Al plate exhibited better thermal spreading performance than conventional Al heat sink.