• Journal of Microbiology and Biotechnology
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• v.19 no.3
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• pp.307-313
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• 2009

In order to systemically investigate the possibility of using coxsackievirus B3 (CVB3) to deliver foreign genes in vivo, a recombinant strain of CVB3 encoding the renilla gene (CVB3-renilla) was constructed. The recombinant CVB3 resulted in extensive and transient expression of the renilla protein within mouse organs, especially the pancreas. The level of expression was generally dependent upon the viral titer present. Moreover, the CVB3-renilla strain was completely attenuated. Interestingly, the recombinant CVB3 vector was expressed much more strongly in mouse organs than was a comparable adenoviral vector. The CVB3-renilla strain did not express the renilla gene in mice with pre-existing coxsackievirus-specific neutralizing antibodies, but direct organ-specific administration of the virus during open-peritoneum surgery was able to circumvent this immunity. This coxsackievirus vector may represent a useful means for delivering and expressing foreign genes in mouse models in an acute and extensive fashion.

• Journal of the Korean Society of Propulsion Engineers
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• v.8 no.2
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• pp.102-114
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• 2004

The present work has been developed the interpretation processor including the behavior of material failure and the separation phenomena under transient dynamic loading (the operation of explosive bolt) using AUTODYN V4.3, SoildWork 2003 and TrueGrid V2.1 programs. It has been demonstrated that the interpretation in ridge-cut explosive bolt under dynamic loading condition should be necessary to the appropriate failure model and the basic stress of bolt failure is the principal stress. The use of this interpretation processor developing the present work could be extensively helped to design the shape and the amount of explosives in the explosive bolt having a complex geometry. It is also proved that the interpretation processor approach is an accurate and effective analysis technique to evaluate the separation mechanism in explosive bolts.

• The Transactions of the Korean Institute of Power Electronics
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• v.8 no.4
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• pp.351-358
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• 2003

This paper describes distribution characteristics of switching surge voltage in stator windings of induction motor driven by IGBT PWM inverter. To analyze the voltage distribution between the turns and coils of stator winding, equivalent circuit model of induction motor including cable was proposed and high frequency parameter is computed by using finite-element method (FEM). From the electro-magnetic transient program (EMTP) simulation of the whole system for induction motor, feeder cable, and PWM inverter, the variable effect on rising time of the inverter, cable length, and switching frequency on the voltage distribution is also presented. In order to experiment, an induction motor, 380[V], 50[HP], with taps from one phase are built to consider the voltage distribution so that these results can be helpful when filter was designed to remove high dv/dt.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.39 no.8
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• pp.653-660
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• 2015

In this study, we conduct power performance and load analyses of two different types of vertical-axis tidal-current turbines using the computational fluid dynamics (CFD) method. To analyze the power output and loads, we perform transient CFD simulations considering the cavitation model using ANSYS CFX. The averaged power output of an H-type rotor was 7.47 kW and 67.6 kW in normal and extreme operating conditions, respectively, which did not satisfy the initial design conditions. However, in the case of the helical-type rotor, the power output under normal and extreme conditions were close to the expected values. The cavitation, which may cause instantaneous power fluctuation, occurred repeatedly at the suction side of the rotors. In order to guarantee a more stable power supply and to prevent fatigue failure, we require a design that minimizes cavitation.

• Journal of Advanced Marine Engineering and Technology
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• v.40 no.5
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• pp.447-452
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• 2016

The use of gas as fuel, particularly liquefied natural gas (LNG), has increased in recent years owing to its lower sulfur and particulate emissions compared to fuel oil or marine diesel oil. LNG is a low temperature, volatile fuel with very low flash point. The major challenges of using LNG are related to fuel bunkering, storing, and handling during ship operation. The main components of an LNG fuel system are the bunkering equipment, fuel tanks, vaporizers/heaters, pressure build-up units (PBUs), and gas controlling units. Low-pressure dual-fuel (DF) engines are predominant in small LNG-powered vessels and have been operating in many small- and medium-sized ferries or LNG-fueled generators.(Tamura, K., 2010; Esoy, V., 2011[1][2]) Small ships sailing at coast or offshore rarely have continuous operation at constant engine load in contrast to large ships sailing in the ocean. This is because ship operators need to change the engine load frequently due to various obstacles and narrow channels. Therefore, controlling the overall system performance of a gas supply system during transient operations and decision of bunkering time under a very poor infrastructure condition is crucial. In this study, we analyzed the fuel consumption, the system stability, and the dynamic characteristics in supplying fuel gas for operating conditions with frequent engine load changes using a commercial analysis program. For the model ship, we selected the 'Econuri', Asia's first LNG-powered vessel, which is now in operation at Incheon Port of South Korea.

• Tunnel and Underground Space
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• v.8 no.3
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• pp.184-193
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• 1998

In order to dispose radioactive wastes safely, it is needed to understand the mechanical, thermal, fluid behavior of rockmass and physico-chemical interactions between rockmass and water. Also, the knowledge about mechanical and hydraulic properties of rocks is required to predict and to model many conditions of geological structure, underground in-situ stress, folding, hot water interaction, intrusion of magma, plate tectonics etc. This study is based on researches about rock mechanics issues associated with a waste disposal in deep rockmass. This paper includes the mechanical and hydraulic behavior of rocks in varying temperature conditions, thermo-hydro-mechanical coupling analysis in rock mass and deformation behavior of discontinuous rocks. The mechanical properties were measured with Interaken rock mechanics testing systems and hydraulic properties were measured with transient pulse permeability measuring systems. In all results, rock properties were sensitive to temperature variation.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.30 no.5
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• pp.79-87
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• 2002

Acoustic responses to pressure oscillations in axisymmetric combustion chamber are numerically investigated to examine the qualitative trend of acoustic instability in liquid rocket engine. Chamber operating condition and excitation frequency of oscillating pressure are selected as exciting parameters of acoustic instability. Artificial perturbation is simulated by total-pressure oscillation with sine wave at chamber inlet. Many approximations and simplifications are introduced without losing the essence of acoustic pressure response. First, steady-state solution for each operating condition is obtained and next, transient analysis is conducted. Depending on operating condition and excitation frequency, the distinct response characteristics are brought. Weak-strength flames and high-frequency excitation tend to cause sensitive acoustic pressure response leading to unstable pressure field. These results are analyzed based on the correlation with acoustic pressure responses from the previous works adopting laminar flamelet model.

• Nuclear Engineering and Technology
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• v.40 no.3
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• pp.199-212
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• 2008

A small-break loss of coolant accident (SB-LOCA) test with a break size equivalent to a 3-inch cold leg break of the APR1400 was carried out as the first transient integral effect test using the ATLAS (Advanced Thermal-hydraulic Test Loop for Accident Simulation). This was the first integral effect test to investigate the integral performance of the test facility and to verify its simulation capability for one of the design-basis accidents. Reasonably good thermal hydraulic data was obtained so that an integral performance of the fluid sub-systems was identified and control performance of the ATLAS was confirmed under real thermal hydraulic conditions. Based on the measured data, a post-test calculation was carried out using the best-estimate thermal hydraulic safety analysis code, MARS 3.1, and the similarity between the expected and actual data was investigated. On the whole, the post-test calculation reasonably predicts the major thermal hydraulic parameters measured during the SB-LOCA test. The obtained data will be used to enhance the simulation capability of the ATLAS and to improve an input model of the ATLAS for simulation of other target scenarios.

• Tunnel and Underground Space
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• v.12 no.3
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• pp.142-151
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• 2002

Claesson(2001)'s analytical solution, and two numerical models with Dirichlet and Neuman interior boundary condition respectively were investigated to estimate the transient temperature distribution with distances from the Taejon underground food cold storage pilot cavern. Claesson's solution, which is based on constant temperature boundary condition at the rock wall during a temperature decline step, showed relatively good agreement with temperature measurements in the rock mass in order of average error difference, 0.89$\^{C}$ without any adjustments on laboratory thermal properties to represent the rock mass. For the numerical model with heat flux through the rock wall, a boundary condition setting technique was newly proposed to overcome the difficulty of prescribing variable convective heat tranfer coefficient and far-field air temperature inside the cavern as they may be certainly changed according to the cooling-down time. The results showed also good agreement with measurements in order of average error difference, 1.58$\^{C}$, and were compared to those of the numerical model with fixed temperature at the rock wall. Finally, the most proper procedure to precisely predict the temperature profile around a cavern was proposed as a series of analysis steps including an analytical exact solution and numerical models.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.33 no.3
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• pp.997-1005
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• 2013

Since the cross-sectional shape of the Nakdong river is compound type, the water stage rises up to the top of the flood plane, as the flow discharge increases during the extreme rain storm in summer. The recent increase of rainfall intensity and flood frequency results in the immersions of parks and hydrophilic facilities located in the flood plain. Therefore it is necessary to analyze the hydraulic characteristics evolved by the extreme rain storm in the flood plain. The study reach ranging from the Gangjeong Goryeong Weir and the Dalseong Weir, where several hydraulic facilities are located along the channel, was selected and numerical simulations were conducted for 42 hours including the peak flood of the typhoon Sanba. The 2-D transient model, FaSTMECH was employed and the accuracy of the model was assessed by comparing the water level between the simulation results and the measured ones at a gauging station. It showed a high correlation with $R^2$ of 0.990, AME of 0.195, and RMSE of 0.252. In addition, the inundation time, the inundation depth, the inundation velocity, and the shear stress variation in the flood plain facilities were analyzed.