• Journal of Venture Innovation
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• v.3 no.1
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• pp.101-115
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• 2020

In the era of the 4th Industrial Revolution customers living began to come out, not inside the purchase funnel. Due to the diversity of product selection and the increase in digital channels, the way customers search for information and purchase it is changing innovatively. So, the customer journey in the digital age is much more complicated than the traditional funnel model suggests. Unlike many previous studies, this study was conducted for 1,200 customers in four product groups of fashion, automobile, cosmetics, and online shopping malls. As a result of the study, we investigated how digital self-efficacy plays a role in purchasing in a series of processes in which digital experience affects customer satisfaction and finally affects purchase. As a theoretical implication, as a result of introducing and testing digital self efficacy as moderated mediation effect. the digital self-efficacy between customer satisfaction and customer loyalty were determined to play a moderated mediation effect role. As a practical implication, it was necessary to actively utilize digital marketing for customers with high digital self-efficacy, but it was suggested that customers with low digital self-efficacy need to be careful about digital marketing fatigue.

• Korean Journal of Plant Tissue Culture
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• v.22 no.3
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• pp.175-182
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• 1995

Rice is one of the most successful monocot in regenerating fertile and genetically stable transgenic plants. However there is no report of a rice line developed in Korea that can be used for regeneration of fertile and genetically stable transformants. In this paper we first demonstrate that a Korean variety Nakdongbyeo, is suitable to obtain transgenic rice plants. Protoplasts from embryogenic suspension cultures were co-transformed with HPT (hygromycin phosphotransferase) and GUS ($\beta$-glucuronidase) genes in separate plasmids in the presence of PEG (polyethylene glycol). In 5 independent experiment, the average frequency of calli showing hygromycin resistance were 1.73%. Plantlets were regenerated from the Hy $g^{R}$ calli. The average efficiency of plantlet regeneration was apprbximately 27%. Based on the GUS activities of hygromycin resistant calli, ca.35% of the resistant calli carried active GUS genes. The R0 transgenic plantlets were grown to maturity and Rl seeds were obtained. By examining the in siぉ activity of GUS in Rl seeds and seedlings, we confirmed that the GUS transgene driven by a CaMV 35S (cauliflower mosaic virus) promoter showed proper expression patterns. We also confirmed Mendelian segregation of the HPT transgene in the Rl generation.n.

• Journal of Plant Biotechnology
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• v.40 no.4
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• pp.224-230
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• 2013

Lily is an important cut-flower in Korea and world as well due to it's a variety of flower colors and various sizes of flowers. To develop elite lily cultivars, conventional breeding techniques have been used so far. However, an introduction of tissue culture system in mass propagation of bulbs and regeneration of shoots with a high efficiency is prerequisite at this moment. Especially, growth of bulbs and shoots as well as reduction of browning is critical factor to proliferate bulbs with shoots of superior lines or cultivar in lily. For this purpose, we tried to test whether ascorbic acid, citric acid and silver nitrate in medium to facilitate growth of bulbs and shoots as well as reduction of browning of bulb scales in lily. As a result, ascorbic acid and silver nitrate showed no significant differences compared to control plants in the growth of bulbs and shoots. When bulb scales were treated with $150mg{\cdot}l^{-1}$ of citric acid, formations of shoots and bulbs showed best result. While, bulb scale treated with $100mg{\cdot}l^{-1}$ of citric acid showed the growth of shoot and root as well as increasing of fresh weight compared to other treatments. Regarding the reduction of browning, $150mg{\cdot}l^{-1}$ of ascorbic acid showed the best result with the less than 2%. Although more experiments with several commercial varieties are needed in the future to establish mass propagation of bulbs of lily, results obtained in this study are supposed to provide the basic knowledge and contribution in tissue culture system of lily.

• Microbiology and Biotechnology Letters
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• v.16 no.6
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• pp.494-498
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• 1988

The optimum conditions for glucoamylase production, and ethanol productivity of the transformant TSD-14 were investigated as compared with the parental strains. The properties of TSD-14 were comparatively similar to the donor S. diastaticus IFO 1046 as regards the conditions of glucoamylase production and ethanol productivity. The soluble starch was the most effective carbon source for the glucoamylase production. While inorganic nitrogen sources did not prompt cell growth and enzyme production, the organic nitrogen sources generally enhanced both cell growth and glucoamylase production. The metal salts such as FeSO$_4$, MgSO$_4$, MnCl$_2$, and NiSO$_4$were favorable to the enzyme production. And the optium temperature and initial pH for glucoamylase production were 3$0^{\circ}C$ and 5. The transformant TSD-14 produced 8.3%(v/v) ethanol from 15% sucrose medium, 4.8%(v/v) ethanol from 15% soluble starch medium, and 7.5%(v/v) ethanol from 15% liquefied potato starch medium. The corresponding fermentation efficiency were 84% , 45% and 70%, respectively.

• Korean Journal of Cognitive Science
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• v.7 no.1
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• pp.57-74
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• 1996

This paper presents a comparison of recognition performance of the features used inthe recent handwritten korean character recognition.This research aims at providing the basis for feature selecion in order to improve not only the recognition rate but also the efficiency of recognition system.For the comparison of feature performace,we analyzed the characteristics of theose features and then,classified them into three rypes:global feature(image transformation)type,statistical feature type,and local/ topological feature type.For each type,we selected four or five features which seem more suitable to represent the characteristics of korean alphabet,and performed recongition experiments for the first consonant,horizontal vowel,and vertical vowel of a korean character, respectively.The classifier used in our experiments is a multi-layered perceptron with one hidden layer which is trained with backpropagation algorithm.The training and test data in the experiment are taken from 30sets of PE92. Experimental results show that 1)local/topological features outperform the other two type features in terms of recognition rates 2)mesh and projection features in statical feature type,walsh and DCT features in global feature type,and gradient and concavity features in local/topological feature type outperform the others in each type, respectively.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.8
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• pp.8-16
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• 2016

This study presents the visualization of shape information based on Octree using 3D laser scanning. The process of visualization was established to construct the Octree structure from the 3D scan data. The scan data was converted to a 2D surface through the mesh technique and the surface was then converted to a 3D object through the Raster/Vector transformation. The 3D object was transmitted to the Octree Root Node and The shape information was constructed by the recursive partitioning of the Octree Root Node. The test-bed was selected as the steel bridge structure in Sungkyunkwan University. The shape information based on Octree was condensed into 89.3%. In addition, the Octree compressibility was confirmed to compare the shape information of the office building, a computer science campus in Germany and a New College in USA. The basis is created by the visualization of shape information for double-deck tunnel and it will be expected to improve the efficiency of structural health monitoring and maintenance.

• Journal of Plant Biotechnology
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• v.37 no.2
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• pp.125-132
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• 2010

Rice is the staple food of more than 50% of the worlds population. Cultivated rice has the AA genome (diploid, 2n=24) and small genome size of only 430 megabase (haploid genome). As the sequencing of rice genome was completed by the International Rice Genome Sequencing Project (IRGSP), many researchers in the world have been working to explore the gene function on rice genome. Insertional mutagenesis has been a powerful strategy for assessing gene function. In maize, well characterized transposable elements have traditionally been used to clone genes for which only phenotypic information is available. In rice endogenous mobile elements such as MITE and Tos (Hirochika. 1997) have been used to generate gene-tagged populations. To date T-DNA and maize transposable element systems has been utilized as main insertional mutagens in rice. A main drawback of a T-DNA scheme is that Agrobacteria-mediated transformation in rice requires extensive facilities, time, and labor. In contrast, the Ac/Ds system offers the advantage of generating new mutants by secondary transposition from a single tagged gene. Revertants can be utilized to correlate phenotype with genotype. To enhance the efficiency of gene detection, advanced gene-tagging systems (i.e. activation, gene or enhancer trap) have been employed for functional genomic studies in rice. Internationally, there have been many projects to develop large scales of insertionally mutagenized populations and databases of insertion sites has been established. Ultimate goals of these projects are to supply genetic materials and informations essential for functional analysis of rice genes and for breeding using agronomically important genes. In this report, we summarize the current status of Ac/Ds-mediated gene tagging systems that has been launched by collaborative works from 2001 in Korea.

• The Journal of the Korea Contents Association
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• v.12 no.6
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• pp.518-529
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• 2012

Integration of Europe was started when European Union Treaty was concluded at Maastricht for the first time on December, 1991. Europe which may be called as a cradle of modern national state has realized a single Europe not only in the socio-economic integration field but also in the political field. Under this background, it is considered that life-long education policy for developing a new integrated growth engine of EU requires educational response that may get ready for socio-economic environmental transformation more than anything else. In particular, this policy is faced with an important task of having to achieve harmony of efficiency through diversity and mutual coordination in pursuing cooperation and integrated development of life-long education field. However, notwithstanding their efforts, since 2008, some countries of EU were faced with economic crisis due to economic recession and this situation starts to drive the whole Europe even to the point of their financial crisis at last. This crisis is currently shaking socio-economic integration of EU. This study intends to observe a status of establishing life-long education system and promoting a policy for socio-economic integration of EU and to analyze as to what kind of relevance adult participation rate of life-long learning among the countries belonged to EU has with per capita income and to explore as whether socio-economic integration among member countries could be sustained based on problems of integrative life-long education system under the economic crisis of EU. In addition, through this study, an implication required for presenting a new paradigm conversion, policy establishment and development direction for the life-long education of our country is intended to be deduced.

• Journal of Korean Society of Environmental Engineers
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• v.31 no.10
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• pp.927-932
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• 2009

Toxic and recalcitrant organic pollutants in wastewaters can be effectively treated when advanced oxidation and biodegradation are combined, ideally with intimate coupling, in which both processes occur simultaneously in the same system. One means to achieve intimate coupling is to coat nanoscale $TiO_2$ on the outside of macroporous biofilm carriers. This study investigated the kinetics of photocatalysis with $TiO_2$-coated porous carriers. The carriers were made of polyvinyl alcohol (PVA) and coated with $TiO_2$ using a low-temperature sol-gel process. The $TiO_2$-coated carriers catalyzed the oxidation of methylene blue (MB) effectively under irradiation of UV light. The overall reaction rate with adsorption and photolysis saturated at high MB concentration, and approached the adsorption rate, which was first order for all MB concent rations. This result indicates that adsorbed MB may have slowed photocatalysis by blocking active sites for photocatalysis. The overall kinetics could be described by a quasi-Langmuir model. The estimated maximum specific (per unit mass of $TiO_2$) transformation rate of MB by the $TiO_2$-coated carriers was four times larger than that obtained from slurry-$TiO_2$ reactors. This observation demonstrated that the $TiO_2$ present as a coating on the carriers maintained high efficiency for transforming recalcitrant organic matter via photocatalysis. These findings serve as a foundation for advancement of an intimate coupling of photocatalysis to biodegradation.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.37 no.3
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• pp.247-256
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• 2011

Existing organic UV protection materials seem to be problematic due to their penetration and irritation to skin. Inorganic UV protection materials are also at issue for safety of their nano-type transformation. Therefore, the recent studies of UV protection materials have been focused not only on the effectiveness but also on their safety. One of the UV protection materials in study which have higher safety is the organic-inorganic conjugation type UV protection material. Previously, we have reported the manufacturing process, physical property and UV protection efficiency of methoxychinnamidoprophy poloysilsesquixan as a new cross-linked polymer type UV protection material. In this study, we have evaluated the effect of the methoxychinnamidoprophy poloysilsesquixan on embryo-fetal development in SD rats. This study is expected to show some definite information related to the effect on pregnancy or embryo-fetal abnormality in case of the clinical exposure of the methoxychinnamidoprophy poloysilsesquixan.