• Journal of Microbiology and Biotechnology
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• v.17 no.7
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• pp.1208-1212
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• 2007

Production of extracellular epothilone B, one of the potent anticancer agents, by free and immobilized Sorangium cellulosum was studied using the repeated batch culture process. The concentration of alginate used in immobilization was directly related to the mass transfer rate of nutrients, mechanical stability, and the epothilone B production yield. With the optimized 3% (w/v) calcium alginate carrier, a prolonged repeated batch culture was investigated for the 5 repeated batches for 24 days. The maximum productivity of epothilone B obtained from the alginate-immobilized cells was 5.03 mg/l/day, which is 3 times higher than that of free cells (1.68 mg/l/day).

• Journal of the Korea Organic Resources Recycling Association
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• v.24 no.1
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• pp.31-40
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• 2016

In this study, mesophilic anaerobic digestion of source separated food waste was carried out by leachate recirculation system and methane gas was produced. Two systems - system A and B were fabricated and placed within water bath to maintain $36^{\circ}C$. Each system was comprised of an anaerobic bioreactor and a leachate tank. Leachate in bioreactor was separated through the screen located at 30 mm above the bottom and a pump was installed to transfer collected leachate to the leachate tank. Everyday, 2.5 L of the leachate was pumped from the bioreactor to the leachate tank for 30 min and transferred leachate was pumped back to the top of the bioreactor for 30min, sequentially. Source separated food waste used for this experiment was washed by water before transferring to the laboratory. Transferred food waste was warmed to $36^{\circ}C$ before being fed to bioreactors. System A was fed to 49.1 g VS (Volatile Solids) and System B was fed to 54.0 g VS at every two weeks, respectively. $NH_4{^+}-N$ and salinity were monitored to see the inhibition toward anaerobic bioreaction and it was found that concentrations of these materials were not high enough to affect the bioreaction. Although the food waste was fed biweekly for 112 days and 140 days at system A and B, respectively, there was no sludge withdrawal from each system. Average methane productions rates were 0.439 L $CH_4/g$ VS and 0.368 L $CH_4/g$ VS for system A and B, respectively.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.28 no.1
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• pp.24-30
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• 2004

Analysis model for the two-phase catalytic reactor is presented. With the progress in development of micro thermofluidic devices, needs fur understanding of the phenomena in two phase reaction in cm scale has been arisen. To investigate thermal and reactive performance of down scaled two phase reactor simple analysis model that is a kind of lumped flow model is proposed. Analysis model presented is based on the experiment on mm scale model reactor. Target experiment is catalytic decomposition of 70wt％ hydrogen peroxide with existence of perovskite L $a_{0.8}$S $r_{0.2}$Co $O_3$ catalyst. It is composed of balance equations of mass and energy. Each phase is considered to be a species fur the simplicity. Axial diffusion and transversal distribution of properties are neglected. Two phase catalytic reaction is modeled as successive gasification of liquid lump around catalyst and reaction in gas phase. Heat transfer is modeled by model function ofNu number. Modeled Nu is expressed as Nu=N $u_{0}$ (1＋ $a_1$( $a_2$ $T^{-}$ $a_3$)exp( $a_4$ $T^{-1}$)exp( $a_{5}$ z). Transfer coefficients are determined by the comparison of experimental results. With the model, heat transfer characteristics are investigated. Also by the mass transfer coefficient, characteristics in mass transfer is investigated. With the result basic understanding on design and analysis of mm scale two-phase reactive device is obtained. Also it can be further applied to micro scale reactive device fabricated by micromachining.ing..

• Transactions of the Korean Society of Mechanical Engineers B
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• v.37 no.4
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• pp.377-385
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• 2013

To identify the effects of an outflow area on pool boiling heat transfer in a vertical annulus, three different flow restrictors were studied experimentally. For the test, a heated tube of smooth stainless steel and water at atmospheric pressure were used. Both annuli with open and closed bottoms were considered. To validate the effects of the outflow area on the heat transfer, the results of the annulus with the restrictor were compared with the data for the plain annulus without the restrictor. The reduction of the outflow area ultimately results in a decrease in the heat transfer. As the outflow area is very small, a slight increase in heat transfer is also observed. The major cause of this tendency is explained as the difference in the intensity of liquid agitation cause by the movement of coalesced bubbles. It is identified that the convective flow, pulsating flow, and evaporative mechanism are considered as the important mechanisms.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.41 no.11
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• pp.719-726
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• 2017

An experimental study was performed to investigate the combined effects of the pitch and heat flux of nearby tubes on boiling in a pool as well as the heat transfer from a horizontally-installed tube bundle. For this test, two smooth stainless steel tubes (19 mm outside diameter) were used, and the water was at atmospheric pressure. The pitch of these tubes was varied between 28.5 mm and 95 mm, and the heat flux of the nearby tube altered between 0 and $90kW/m^2$. Enhancements in heat transfer were clearly observed when the heat flux of the nearby tube increased while the heat flux of the test section remained below $40kW/m^2$. The tube pitch was found to have a negligible effect on heat transfer when the pitch was greater than four times larger than the tube diameter. The circulating flow, convective flow, and liquid agitation were all seen to enhance heat transfer; however, the interaction between the flow and coalescence of bubbles was detrimental to heat transfer.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.57 no.8
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• pp.1412-1415
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• 2008

A glucose biosensor using a microdisk resonator in polymeric waveguides was developed by observing either the shift in the resonant wavelength or the variation in the optical power. The deformation in the transfer curve of the vertically coupled resonator sensor resulting from the variation in the disk-to-ring coupling, which was incurred by the application of the target analyze, was suppressed. And the refractive index of the polymeric waveguide was devised to closely follow that of the analyze itself for enhancing the sensitivity of the sensor. The sensitivity and measurement range were observed to be respectively 0.14 pm/(mg/dL) and 1500 mg/dL (theoretically up to 4700 mg/dL, for the wavelength shift method and 0.04 dB/(mg/dU and 140 mg/dL the power variation scheme.

• Korean Journal of Plant Tissue Culture
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• v.22 no.1
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• pp.7-11
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• 1995

Protoplasts were isolated from hypocotyl tissues of 5-day-old Brassica oleracea var capitata Green Challenger seedlings. Several media were used for protoplast culture and shoot regeneration. The shoot-regeneration rapacity of protoplast derived callus depended on the initial culture medium. Protoplasts were cultured in liquid medium (B5 medium supplemented with CaCl2, 2H2O 600mg/L, g1ucose 20g/L, D-mannito1 70g/L, NAA lmg/L, BA lmg/L, 2.4-D 0.25 mg/L)at 27$^{\circ}C$ under the dark After 5 to 10 days, cultlues were diluted with medium with a reduced osmotic stabilizer and then transferred to illuminated conditions. The culture medium was changed with the fresh medium at 7- to 10-day-intervals until the formation of microcallus. Hypocotyl protoplast-derived callus proliferated when transferred to MS medium supplemented with NAA lmg/L, BA 1mg/L and GA$_3$ 0.02mg/L. Upon transfer to MS basal medium without growth regulators, roots were produced. In an attempt to increase the regeneration frequency, 10g/L polyvinylpyrrolidone was added to the regeneration medium, but the shoot regeneration was mot improved. The regenerated whole plants were acclimated in a sterized soilless mixture(vermiculite 2;perlite 2;peat moss1) in a culture room.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.25 no.9
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• pp.1183-1192
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• 2001

The numerical simulation has been conducted for the investigation of flow and heat transfer characteristics of an oblique impingement jet injected to a flat plate. The finite volume method was used to discretize the governing equations based on the non-orthogonal coordinate with non-staggered variable arrangement. The $textsc{k}$-$\varepsilon$-ν(sup)'2 turbulence model was employed to consider the consider the anisotropic flow characteristics generated by the impingement jet flow. The predicted results were compared with the experimental data and those of the standard $textsc{k}$-$\varepsilon$ turbulence model. The results of the $textsc{k}$-$\varepsilon$-ν(sup)'2 model showed better agreement with the experimental data than those of the standard $textsc{k}$-$\varepsilon$ model. In order to get the optimum condition, the flow and temperature fields were calculated with a variation of inclined angle($\alpha$=30$^{\circ}$~90$^{\circ}$) and the distance between the jet exit and impingement plate-to-diameter (L/D=4~10) at a fixed Reynolds number(Re=20,000). For a small L/D, the near-peak Nusselt numbers were not significantly effected by the inclined angle. The near-peak Nusselt numbers were not significantly affected by the L/D in the case of a large $\alpha$. The overall shape of the local Nusselt numbers was influenced by both the jet orifice-to-plate spacing and the jet angle.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.41 no.10
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• pp.659-665
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• 2017

When an electrically conducting fluid flows through a staggered tube bank, the heat transfer and fluid flow features are changed by the externally introduced magnetic field. This study provides a numerical investigation of this phenomenon. Heat and fluid flows are investigated for unsteady laminar flows at Reynolds numbers of 50 and 100 with the Hartmann number gradually increasing from zero to 100. As the Hartmann number increases, and owing to the effects of the introduced magnetic field, the velocity boundary layer near the tube wall is thinned, the flow separation is delayed downstream, and the shrinkage of a recirculation zone formed near the rear side is observed. Based on these thermo-fluid deformations, the resulting changes in the local and average Nusselt number are investigated.

• THE INTERNATIONAL COMMERCE & LAW REVIEW
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• v.14
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• pp.177-218
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• 2000

Global Electronic Trading means that trading partners trade each other via Global Electronic Trading Network, that is, global business to business electronic commerce. Recently, where the cargo arrives ahead of the B/L, the importer cannot take delivery of the cargo without the B/L. This situation is referred to as the B/L dilemma. But the BOLERO system will resolve this B/L dilemma. Bolero Project is developing a cross industry utility platform for the secure, electronic transfer of commercial trade information world wide. After the successful testing of an original pilot project, The Bolero Association was formed in 1995 by a group of interested cross industry companies. As a joint venture between SWIFT and TT Club, bolero.net is changing the way the world trades by providing a web-based, paperless mode of commerce that is designed to become a global standard. Bolero International Limited published the first edition of Bolero Rulebook in 1999. The Bolero Rulebook as amended from time to time, governing the relationship between Users and their rights and obligations arising from the Bolero system. The Bolero service will be governed by a multilateral contract called the Bolero Rule Book which specifies the rights and responsibilities of Bolero and its users. The Title Registry and Bolero Bill of Lading provide a fully functional equivalent to the paper bill of lading. The Bolero Bill of Lading can be created, transferred, amended, and surrendered by way of designating to order party, blank endorsement, refusal by the transferee etc. Thus, this study deals with the functions of Bolero Bill of Lading and the problems and solutions in the Bolero Bill of Lading in point of definitions and operation under the Bolero Rulebook.