• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2005년도 International Meeting of the Microbiological Society of Korea
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• pp.217.2-217
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• 2005

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2005년도 International Meeting of the Microbiological Society of Korea
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• pp.215.1-215
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• 2005

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.493-494
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• 2000

• 생약학회지
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• 제35권4호통권139호
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• pp.324-329
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• 2004

SKT is consisted of skullcap radix, knope-sedge radix and trametes mushroom. SKT mixture extract has been used for curing breast cancer and cervical cancer as a folk medicine without any kind of experimental evidence to support the rationales for its clinical use. This study was undertaken to investigate the antitumor effects and toxicity of SKT. Tumor was induced by implantation of B16F10 melanoma cells $(1{\times}10^6\;cells/mouse)$ into abdominal skin in ICR mice and SKT application (5 mg/mouse, p.o.) was initiated 4 days prior to tumor induction and lasted for 42 days. SKT significantly inhibited not only tumor growth but also metastasis of i.v. implanted melanoma cells into lung and showed prolonged life span of tumor bearing mice. The combined theraphy of SKT with doxorubicin was more effective against tumor metastasis into lung. SKT almostly recovered serum SGPT to normal level of galactosamine/LPS-induced hepatitis mice. High dose of SKT did not show any acute side effects. But, in vitro SKT did not inhibit the growth of melanoma cells, which suggests that the antitumor effects of SKT might be menifested by indirect mechanisms.

• Journal of Microbiology and Biotechnology
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• 제14권6호
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• pp.1190-1195
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• 2004

In recent years, there has been an intensive research on decolorization of dye and textile wastewater by various fungal strains. In this study, the decolorization ability of three commercial dyes, acid yellow 99, acid blue 350, and acid red 114, were investigated using 10 fungal strains. Among the fungal strains tested, Trametes versicolor KCTC 16781 completely decolorized all dyes in both solid and liquid experiments, and was also able to decolorize the mixture of those three dyes in liquid experiments. The secretion of the ligninolytic enzymes into the extracellular medium during decolorization by T versicolor KCTC 16781 was also studied. No lignin peroxidase activity was detected, and manganese peroxidase and laccase activities were investigated.

• Journal of Forest and Environmental Science
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• 제34권2호
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• pp.126-135
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• 2018

Wood decay fungi were collected in 47 different locations in eight provinces of South Korea from 2011 to 2013. One thousand and five hundreds three fruiting bodies of wood-decay fungi were collected, identified, and classified into 2 phyla, 7 classes, 19 orders, 56 families, 159 genera and 365 species. The most dominant genus and species found were Trametes and T. versicolor. The highest species diversity was found in broad-leaved forest (273 species), and was also found at elevations of 500-1,000 m (227 species). A total of 333 species were collected from broad-leaved trees, 87 species from coniferous trees, and 55 species were collected from both forest types. Gymnopilus liquiritiae was the most dominant species in coniferous trees, while T. versicolor, which was mostly collected from tree trunks below 500 m in elevation, was dominant in broad-leaved trees. Results from the quantitative cluster analysis of wood decay fungi showed that the highest species diversity index was 1.80 in the mixed forests, while the highest similarity among forest types was shown between the broad-leaved and mixed forests.

• Mycobiology
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• 제47권4호
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• pp.506-511
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• 2019

We investigated the antifungal activities of an endophytic fungus identified as Acaromyces ingoldii, found on a loblolly (Pinus taeda L.) pine bolt in Louisiana during routine laboratory microbial isolations. The specific objectives were to determine the inhibitory properties of A. ingoldii secondary metabolites (crude extract) on the mycelial growth of a brown-rot fungus Gloeophyllum trabeum and a white-rot fungus Trametes versicolor, and to determine the effective concentration of A. ingoldii crude preparation against the two decay fungi in vitro. Results show the crude preparation of A. ingoldii from liquid culture possesses significant mycelial growth inhibitory properties that are concentration dependent against the brownrot and white-rot fungi evaluated. An increase in the concentration of A. ingoldii secondary metabolites significantly decreased the mycelial growth of both wood decay fungi. G. trabeum was more sensitive to the inhibitory effect of the secondary metabolites than T. versicolor. Identification of specific A. ingoldii secondary metabolites, and analysis of their efficacy/specificity warrants further study. Findings from this work may provide the first indication of useful roles for Acaromyces species in a forest environment, and perhaps a future potential in the development of biocontrol-based wood preservation systems.

• Journal of Microbiology and Biotechnology
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• 제25권8호
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• pp.1361-1370
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• 2015

A blue laccase was purified from a white rot fungus of Trametes trogii, which was a monomeric protein of 64 kDa as determined by SDS-PAGE. The enzyme acted optimally at a pH of 2.2 to 4.5 and a temperature of 70℃ and showed high thermal stability, with a half-life of 1.6 h at 60℃. A broad range of substrates, including the non-phenolic azo dye methyl red, was oxidized by the laccase, and the laccase exhibited high affinity towards ABTS and syringaldazine. Moreover, the laccase was fairly metal-tolerant. A high-molecular-weight kraft lignin was effectively polymerized by the laccase, with a maximum of 6.4-fold increase in weight-average molecular weight, as demonstrated by gel permeation chromatography. Notable structural changes in the polymerized lignin were detected by Fourier transform infrared spectroscopy and ¹H NMR spectroscopy. This revealed an increase in condensed structures as well as carbonyl and aliphatic hydroxyl groups. Simultaneously, phenolic hydroxyl and methoxy groups decreased. These results suggested the potential use of the laccase in lignin modification.

• 미생물학회지
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• 제36권3호
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• pp.192-195
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• 2000

백색부후균류 laccase의 아미노 말단과 카복시 말단에 잘 보존된 구리결합부위를 암 호화하는 DNA 염기서열과 상보적인 primer를 이용하여 백색부후균의 일종인 영지버섯 Gandoderma lucidum에서 laccase 유전자 단편을 분리하였다. PCR 로 증폭하여 클로닝한 1.6 Kb DNA 절편의 염기 서열을 결정하여 분석하였다. 이 DNA에는 7개의 인트론이 존재 하였으며 엑손의 염기서열과 이로부터 추정된 아미노산 서열은 Tranmetes villosa laccase(lccl)와 각각 47%, 79% 동일하였다. 기타 다른 백색부후균류의 laccase 아미노산 서 열과는 66~78% 동일하였다.

• Advances in environmental research
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• 제9권2호
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• pp.85-95
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• 2020

In this study, the decolorization of Evercion Blue P-GR (EBP) and Ostazin Black H-GRN (OBH) was investigated using white-rot fungi named as Trametes versicolor (T. versicolor) by Membrane Bioreactor (MBR) system. This study involved experiments employing synthetic textile wastewater in Membrane Bioreactor (MBR) system (170 ml), initially inoculated with a pure culture of fungi, but operated, other than controlling pH (4.5±0.2) and temperature (25±1℃), under non-sterile conditions. The effect of dye concentrations on fungal biodegradation was also investigated. The decolorization efficiencies were 98%, 90%, and 87% respectively, for EBP when the initial dye concentration of 50, 100, and 200 mg L^-1 were used. However, the decolorization percentages for OBH dye were obtained 95% for 50 mg L^-1 dye solution in 2 days and 66% for 100 mg L^-1 dye solution in 5 days. Possible interactions between dye molecules and the fungal surface were confirmed by SEM, EDX, and FTIR analyses.