Mycobiology

The Korean Society of Mycology (한국균학회)
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Effect of Acaromyces Ingoldii Secondary Metabolites on the Growth of Brown-Rot (Gloeophyllum Trabeum) and White-Rot (Trametes Versicolor) Fungi

  • Olatinwo, Rabiu (USDA Forest Service, Southern Research Station) ;
  • So, Chi-Leung (School of Renewable Natural Resources, LSU AgCenter) ;
  • Eberhardt, Thomas L. (USDA Forest Service, Forest Products Laboratory)
  • Received : 2019.05.11
  • Accepted : 2019.10.28
  • Published : 2019.12.01
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Abstract

We investigated the antifungal activities of an endophytic fungus identified as Acaromyces ingoldii, found on a loblolly (Pinus taeda L.) pine bolt in Louisiana during routine laboratory microbial isolations. The specific objectives were to determine the inhibitory properties of A. ingoldii secondary metabolites (crude extract) on the mycelial growth of a brown-rot fungus Gloeophyllum trabeum and a white-rot fungus Trametes versicolor, and to determine the effective concentration of A. ingoldii crude preparation against the two decay fungi in vitro. Results show the crude preparation of A. ingoldii from liquid culture possesses significant mycelial growth inhibitory properties that are concentration dependent against the brownrot and white-rot fungi evaluated. An increase in the concentration of A. ingoldii secondary metabolites significantly decreased the mycelial growth of both wood decay fungi. G. trabeum was more sensitive to the inhibitory effect of the secondary metabolites than T. versicolor. Identification of specific A. ingoldii secondary metabolites, and analysis of their efficacy/specificity warrants further study. Findings from this work may provide the first indication of useful roles for Acaromyces species in a forest environment, and perhaps a future potential in the development of biocontrol-based wood preservation systems.

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References

  1. Boekhout T, Theelen B, Houbraken J, et al. Novel anamorphic mite-associated fungi belonging to the Ustilaginomycetes: Meira geulakonigii gen. nov., sp. nov., Meira argovae sp. nov. and Acaromyces ingoldii gen. nov., sp. nov. Int J Syst Evol Micr. 2003;53(5):1655-1664. https://doi.org/10.1099/ijs.0.02434-0
  2. Paz Z, Gerson U, Sztejnberg A. Assaying three new fungi against citris mites in the laboratory, and field trial. BioControl. 2007;52(6):855-862. https://doi.org/10.1007/s10526-006-9060-2
  3. Gerson U, Gafni A, Paz A, et al. A tale of three acaropathogenic fungi in Israel: Hirsutella, Meira and Acaromyces. Exp Appl Acarol. 2008;46(1-4):183-194. https://doi.org/10.1007/s10493-008-9202-6
  4. Kushnir L, Paz Z, Gerson U, et al. The effect of three basidiomycetous fungal species on soil-borne, foliage and fruit-damaging phytopathogens in laboratory experiments. BioControl. 2011;56(5):799-810. https://doi.org/10.1007/s10526-011-9341-2
  5. Gao XW, Liu HX, Sun ZH, et al. Secondary metabolites from the deep-sea derived fungus Acaromyces ingoldii FS121. Molecules. 2016;21(4):371. https://doi.org/10.3390/molecules21040371
  6. Jusino MA, Lindner DL, Banik MT, et al. Heart rot hotel: fungal communities in red-cockaded woodpecker excavations. Fungal Ecol. 2015;14:33-43. https://doi.org/10.1016/j.funeco.2014.11.002
  7. Arantes V, Goodell B. Current understanding of brown-rot fungal biodegradation mechanisms: a review. Deteriorat Protect Sustain Biomater. 2014;1158:3-21.
  8. Hatakka A, Hammel KE. Fungal biodegradation of lignocelluloses. In: Hatakka A, Hammel KE, editors. Industrial applications. Berlin, Heidelberg: Springer; 2011. pp. 319-340.
  9. Zhang Z, Yang T, Mi N, et al. Antifungal activity of monoterpenes against wood white-rot fungi. Int Biodeterior Biodegrad. 2016;106:157-160. https://doi.org/10.1016/j.ibiod.2015.10.018
  10. Gardes M, Bruns TD. ITS primers with enhanced specificity for basidiomycetes - application to the identification of mycorrhizae and rusts. Mol Ecol. 1993;2(2):113-118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
  11. White TJ, Bruns T, Lee SJ, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, et al., editors. PCR protocols: a guide to methods and applications. New York: Academic Press, Inc.; 1990. pp. 315-322.
  12. Olatinwo R, Fraedrich S. An Acaromyces species associated with bark beetles from southern pine has inhibitory properties against Raffaelea lauricola, the causal pathogen of Laurel wilt disease of Redbay. Plant Health Prog. 2019;20(4):220-228. https://doi.org/10.1094/php-06-19-0039-rs
  13. Olatinwo R, Fraedrich S. Acaromyces ingoldii inhibits the laurel wilt pathogen, Raffaelea lauricola in vitro. Phytopathology. 2016;106(12):55.
  14. Fraedrich SW, Johnson CW, Menard RD, et al. First report of Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae) and Laurel wilt in Louisiana, USA: the disease continues westward on sassafras. Florida Entomol. 2015;98(4):1266-1268. https://doi.org/10.1653/024.098.0445
  15. Stodulkova E, Cisarova I, Kolarik M, et al. Biologically active metabolites produced by the basidiomycete Quambalaria cyanescens. PloS One. 2015;10(2):e0118913. https://doi.org/10.1371/journal.pone.0118913
  16. Yang DQ, Wan H, Wang XM, et al. Use of fungal metabolites to protect wood-based panels against mould infection. BioControl. 2007;52(3):427-436. https://doi.org/10.1007/s10526-006-9022-8
  17. Jung SJ, Kim NK, Lee DH, et al. Screening and evaluation of Streptomyces species as a potential biocontrol agent against a wood decay fungus, Gloeophyllum trabeum. Mycobiology. 2018;46(2):138-146. https://doi.org/10.1080/12298093.2018.1468056

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