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Effects of Various Light Sources on the Carotenoid and Glucosinolate Contents in Chinese Cabbage (Brassica rapa L. ssp. pekinensis) (다양한 광원이 배추 내 Carotenoid와 Glucosinolate 함량에 미치는 영향)

  • Sung, Ho-Young;Jo, Lee-Kyeong;Chun, Jin-Hyuk;Woo, Hyun-Nyung;Kim, Sun-Ju
    • Korean Journal of Environmental Agriculture
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    • v.37 no.2
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    • pp.79-86
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    • 2018
  • BACKGROUND: Chinese cabbage biosynthesizes various phytochemicals including carotenoids and glucosinolates. Environmental stress has a major effect on the growth and yields of vegetables, and can significantly affect nutritionally important phytochemicals. Phytochemicals of plants are influenced by light, temperature, carbon dioxide, and growing conditions. The aim of this study was to investigate the effect of various light sources on carotenoid and glucosinolate contents in Chinese cabbage. METHODS AND RESULTS: [Experiment I] Set the control (field control, FC) on the ground. Using acrylic sunlight, experiments were set up transparency box (field transparency, FT), red box (field red, FR) and blue box (field blue, FB). [Experiment II] Set the control (chamber control, CC) in the greenhouse. Using plant growth chamber with artificial light, experiments were set up LED red (chamber red, CR), LED blue (chamber blue, CB), LED mixed red+blue (chamber red+blue, CRB) and fluorescent (chamber fluorescent, CF). After plant growth, Chinese cabbage was harvested at 110 days after sowing (DAS). The status of plants growth (leaf length, width, fresh weight etc.) was immediately investigated. Carotenoid and GSL contents were analyzed by HPLC. [Experiment I] Results documented that the ranges of total carotenoid contents were 25.39 ~ 58.80 mg/kg dry wt for lutein, 0.84~ 4.22 mg/kg dry wt for zeaxanthin, and 3.85~18.71 mg/kg dry wt for ${\beta}$-carotene. Lutein was the highest for the content and the largest for the variation as well. [Experiment II] Results documented that the ranges of total carotenoid contents were 24.66~137.96 for lutein, 2.51~20.65 for zeaxanthin, and 8.40~49.80 mg/kg dry wt for ${\beta}$-carotene. The total carotenoid contents of CR (156.62) and CB (115.90) were 1.6~2.3 times larger than the other treatments, and ${\beta}$-carotene content was about twice as high as that of the other treatments on the CR (38.74 mg/kg dry wt.). [Experiment I] Total GSL content was the highest in FT (19.76) that was higher 1.7 times than the lowest treatment ($11.39{\mu}mol/g\;dry\;wt$.). [Experiment II] The total content of GSL was highest in CRB (4.19) and lowest in CF ($2.88{\mu}mol/g\;dry\;wt$.). In the CRB, total GSL contents ($4.19{\mu}mol/g\;dry\;wt$.) was the highest. CONCLUSION: Total and individual carotenoid and GSL contents in Chinese cabbage show significant differences under different light sources. Red and blue lights contribute to significant carotenoids expression and antioxidant activity for nutrition and health benefits. These results concluded that the introduction of varying lights affected the synthesis of important nutrient compounds in Chinese cabbage. It is predicted that the application of good light source enhances the accumulation of functional compounds.

Performance of Broiler Chicks Fed Normal and Low Viscosity Rye or Barley with or without Enzyme Supplementation

  • He, T.;Thacker, P.A.;McLeod, J.G.;Campbell, G.L.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.2
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    • pp.234-238
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    • 2003
  • This experiment was conducted to measure nutrient digestibility and performance in broiler chicks fed diets based on normal and low viscosity rye or barley fed with and without enzyme (pentosanase and $\beta$-glucanase) during a 17 day growth trial. A total of 150 one-day old, male broiler chicks (5 birds per pen and 5 pens per treatment) were randomly assigned to one of six dietary treatments in a $3{\times}3$ factorial design experiment (3 cereals${\times}$2 enzyme levels). Digestibility coefficients were determined using chromic oxide. Digestibility coefficients for dry matter and crude protein were significantly (p=0.0001) higher for the barley-based diets than for any of the rye-based diets. Digestibility coefficients for gross energy did not differ (p>0.05) due to cereal grain. There were no differences in the digestibility coefficients for dry matter and gross energy between chicks fed normal and low viscosity rye. However, the digestibility coefficient for crude protein was higher (p=0.01) for the low viscosity rye compared with the normal viscosity rye. Addition of enzyme to the diet significantly (p=0.0001) increased digestibility coefficients for dry matter, crude protein and energy. There were no significant differences in weight gain, feed intake or feed conversion between birds fed barley or rye or between birds fed normal or low viscosity rye. Enzyme supplementation significantly improved (p=0.0001) weight gain, intake and feed conversion. The overall results of this experiment indicate that unsupplemented barley and rye do not support adequate growth rates in poultry. Enzyme supplementation dramatically improved broiler performance. In addition, genetic selection to reduce the viscosity of rye had only a modest effect on the nutritive value of rye for broilers.

Chemical and Physical Predictors of the Nutritive Value of Wheat in Broiler Diets

  • Ball, M.E.E.;Owens, B.;McCracken, K.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.1
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    • pp.97-107
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    • 2013
  • The aim of this study was to establish relationships between chemical and physical parameters of wheat with performance and digestibilities of feed components in broiler chickens fed on wheat-based diets. Ninety-four wheat samples were selected for inclusion in four bird trials. Birds were housed in individual wire metabolism cages from 7 to 28 d and offered water and feed ad libitum. Dry matter intake (DMI), liveweight gain (LWG) and gain:feed were measured weekly. A balance collection was carried out from 14 to 21 d for determination of apparent metabolizable energy (AME), ME:gain, dry matter retention, oil and neutral detergent fibre (NDF) digestibility. At 28 d the birds were humanely killed, the contents of the jejunum removed for determination of in vivo viscosity and the contents of the ileum removed for determination of ileal dry matter, starch and protein digestibility. When wheat parameters were correlated with bird performance data, it was found that specific weight was not significantly (p>0.05) related to bird performance. Bird DMI, LWG and gain:feed were best correlated (p<0.05) with the rate of starch digestion, although the coefficients of correlation (r) were still low (0.246 to 0.523). A negative relationship (p<0.01) between AME and total (r = -0.432) and soluble (r = -0.304) non starch polysaccharide (NSP) was observed in this study. Thousand grain weight (TG) was positively correlated with DMI (r = 0.299), LWG (r = 0.343) and gain:feed (r = 0.371). When establishing multiple regression relationships, correlation coefficients greater than 0.8 were achieved for DMI, LWG, gain:feed and ileal crude protein digestibility. However, the economics involved in determining the parameters involved in the regressions make the process impractical.

RNA Production from the Hydrolyzate of Sliced and Dried Sweet Potatoes. (절간고구마를 이용한 RNA 생산에 관한 연구)

  • 이종임;송재철;조원대;양한철
    • Microbiology and Biotechnology Letters
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    • v.8 no.1
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    • pp.9-18
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    • 1980
  • During an extensive screening tests of yeasts for their RNA formation, it was found that Cryptococcus laurentii had especially high RNA content and high dry cell weight, when hydrolyzate of sliced and dried sweet potatoes was used as a carbon source. Growth conditions of this strain were examined, and the most desirable results were obtained at 48 hours of cultivation on a reciprocal shaker at 3$0^{\circ}C$ with initial pH 6.0. Under the above conditions, the RNA content and yield of dry cells were investigated using various media compositions. Ammonium sulfate 0.40%, peptone 0.6 %, and yeast extract 0.4% were appeared to be favorable as a nitrogen sources. The optimum concentrations of K $H_2$P $O_4$, M $n^{++}$, C $O^{++}$ were 0.05 %, 0.1 %, and 0.001 %, respectively. Ca-pantothenate, 400$\mu\textrm{g}$/$m\ell$, showed relatively favorable effects as a growth factor. The maximum RNA content obtained in this study was 16.8 % of the total dry cell weight.t.t.

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Effects of Nitrogen Fertilizer Application Level and Cutting Time on Forage Yield and Feed Value of Rye in Paddy Field (질소시비량과 예취시기가 답리작 호밀의 수량 및 사료가치에 미치는 영향)

  • ;Je-Cheon Chae
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.4
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    • pp.373-381
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    • 1994
  • This experiment was conducted to investigate the effects of nitrogen fertilizer level on forage yield and feed value, and to clarify the optimum nitrogen fertilizer level of rye in middle west area when cultured in paddy field. The field experiment treated 5 levels of nitrogen fertilization was carried out at Yesan from Oct. 1990 to June 1991. The fresh and dry weight increased with increased nitrogen fertilizer level by 30kg /10a, but dry matter ratio decreased. The ratio of leaf blade and leaf sheath in rye plant increased with increased nitrogen fertilizer level, but the ratio of stem and inflorescence decreased. The content and yield per unit area for protein, total digestible nutrient(TDN). Minerals, and energy increased with increased nitrogen fertilizer level, while acid detergent fiber(ADF) and neutral detergent fiber(NDF) decreased. The ratio of available protein among crude protein became higher with increased nitrogen fertilizer level. The optimum clipping time for net energy gain(NEG) and net energy maintenance(NEM) were 10 days earlier than milk stage for conventional items including TDN and proteins.

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Effects of Fertilizer Level in Nursery on Dry Stem Yield and Quality of Mat Rush (Juncus effusus L. var. decipiens Buchenan) (골풀 묘상 시비량이 건경 수량과 품질에 미치는 영향)

  • Shin, Dong-Young;Jung, Dong-Soo;Hyun, Kyu-Hwan;Seo, Young-Nam;Lim, June-Taeg;Kwon, Byung-Sun
    • Korean Journal of Plant Resources
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    • v.20 no.4
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    • pp.309-311
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    • 2007
  • This study was conducted to select the most suitable fertilizer level on nursery of mat rush for good quality and high yield. $N-P_{2}O_{5}-K_{2}O=60-30-60$ (kg/10a), fertilizer level on nursery of mat rush showed relatively superior values for all yield components and quality with 57g in fresh weight, 12g in dry weight, 23% in air drying ratio for per plant at the nursery and 125cm in stem length, 150 numbers in stem number, 4,082kg/10a in fresh stem yield, 345kg/10a in long stem yield, 996kg/10a in medium stem yield, 1,421kg/l0a in total stem yield, 34% in dry stem ratio and 30% in long stem ratio at the main paddy field. The results indicate that fertilizer level, $N-P_{2}O_{5}-K_{2}O=60-30-60kg/10a$ in nursery shows different adaptabilities to a particular fertilizer level and 60-30-60 (kg/10a) seems to be the most suitable fertilizer level of mat rush for good quality and high yield.

Nitrogen fixation, and growth characteristics of Three Legume cover crops in no-tillage paddy field

  • Cho, Young-Son
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.48 no.4
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    • pp.308-315
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    • 2003
  • A field experiment was conducted to investigate the performance of three legume species in a zero-tillage, non-fertilized rice field in a temperate zone. Before the experiment for 5 years, from 1995 to 1999, plant growth patterns of three legume species grown as over-wintering (October-May) cover crops on a paddy field were maintained to study N balance and $\textrm{N}_2$ fixation. Decrease in plant density accelerated from after winter to flowering from 1,090, 320, and 5 to 732, 232, and 6 plants $\textrm{m}^{-2}$ in Chinese milk vetch (CMV), white clover (WC), and hairy vetch (HV), respectively. Total dry weights of plants above-ground level were 0.05, 0.11, and 2.43 g $\textrm{plant}^{-1}$. in WC, CMV and HV respectively but steeply increased at ripening up to 0.77, 2.33, and 26 g $\textrm{plant}^{-1}$. The root dry weight of HV and CMV rapidly increased while in WC, root dry weight increased slightly towards flowering. The highest nodule numbers were recorded in CMV to April thereafter WC produced the highest. Nodule size was distributed within 7mm in CMV but it was larger in HV varying from 1 to 10mm. Shoot N (g $\textrm{m}^{-2}$) greatly increased from over-wintering to flowering in CMV, HV and WC and it ranged from 1.66, 0.5 and 1.92 to 12.6, 3.1 and 13.02 g $\textrm{m}^{-2}$, respectively. After wintering, the initial shoot N content (%) was more in CMV. Root N content (%) was constant or slightly decreased in HV and WC. Soil total N in the control plot (clean fallow) was the highest on Mar. 2 then decreased rapidly to flowering. Soil N content was constant in HV plots whereas it was low in WC plots for the entire growth period except just after winter. Maximum nitrogenase activities were 9, 37.8, and 131 mol $\textrm{C}_2\textrm{H}_4$ $\textrm{plant}^{-1}$ $\textrm{hour}^{-1}$. in CMV, HV, and WC, respectively. Nitrogenase activity showed a direct correlation with nodule number, size and fresh weight. As a cover crop preceding a rice crop, CMV is more suited to colder regions due to its earlier ripening characteristics. Hairy vetch and WC are recommended for regions with a mild winter and a long summer owing to their late ripening and great N fixation activity.

Competitive Effects of Allelochemics on the Monoculture and Corss-cropping Culture System of Plants (작물(作物)의 단일(單一) 및 교호(交互) 재배시(栽培時) 알레로파지 특성(特性)에 관(關)한 연구(硏究))

  • Suh, Jang-Sun;Lee, Sang-Kyu
    • Korean Journal of Soil Science and Fertilizer
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    • v.26 no.4
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    • pp.259-264
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    • 1993
  • Allelopathic chemicals exudated from plants stimulate or inhibit crops directly or indirectly. To prove the effects of allelochemics, we isolated and identified the compounds by bioassays on the monoculture and crosscropping cultre systems. p-Coumaric acid were exudated on all of the test crops such as tomato, red pepper, lettuce, chinese cabbage and sesame, but pyrogallol and phenylacetic acid on tomato. hydroquinone on red pepper and egg plant, pyrogallol on lettuce, and vanillic acid on chinese cabbage. The highest total concentration of allelochemics was $5,883{\mu}g$ on tomato, lowest was $220{\mu}g/g$ dry plant weight on sesame. On the cross-cropping culture of tomato-egg plant, tomato-red pepper, chinese cabbage-egg plant, chinese cabbage-red pepper and chinese cabbage-sasame, the plant height, aerial dry weight and total dry weight of the tomato and the chinese cabbage were inereased contrast with monoculture, but decreased greatly on red pepper and sesame. Growth rate of both crops on the cross-cropping culture of tomato-chinese cabbage declined, while that of chinese cabbage was increased but lettuce decreased on the chinese cabbage-lettuce cross-cropping culture contrast with monoculture.

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Effects of Mixing Ratio of Substrates and Concentration of Fertigation in Nutrient Solutions on the Early Growth of Cucumber Plug Seedlings (배지의 혼입비율과 관비 양액 농도가 오이 플러그묘의 초기생장에 미치는 영향)

  • Kim, Hong-Gi;Cho, Ja-Yong;Bae, Jong-Hyang;Yang, Seung-Yul;Heo, Buk-Gu
    • Journal of Bio-Environment Control
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    • v.16 no.2
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    • pp.101-107
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    • 2007
  • This experiment was carried out to develop the substrate mixtures for the production of high quality plug seedlings of cucumber. Peatmoss based substrates, rice hull, carbonized rice hull, decomposed sawdust, perlite and granular rockwool were mixed by five different mixing ratioes (M1, M2, M3, M4 and M5). The cultivars used were cucumber (Cucumis sativus L. cv. Janghyung heukjinju) plants. The higher the content of peatmoss added, the higher the plant growth in terms of plant height, leaf area and total dry weight, which leading to the production of high quality plug seedlings. Seedlings growth of cucumber were greater in M5 mixtures [peatmoss:rice hull:decomposed sawdust=40:40:20(v/v)], M4 mixtures [peatmoss:rice hull:decomposed sawdust:granular rockwool=30:25:20:25(v/v)] and M2 mixtures [peatmoss:rice hull:decomposed sawdust:granular rockwool=20:20:15:25:20(v/v)] The concentrations of nutrient solution (EC) had a great influence on plant height, leaf area, total fresh and dry weight of cucumber seedlings growth. As the concentration of nutrient solution increased from 0.1 to $1.5dS{\cdot}m^{-1}$, the growth and seedling quality of cucumber in plant height, leaf area and dry weight were significantly improved.

Influence of DIF on Factors Associated with Growth of Young Watermelon(Citrullus vulgaris S.) Plant in Controlled Environments (수박의 초기 생장에 미치는 DIF의 영향)

  • 권성환
    • Journal of Bio-Environment Control
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    • v.5 no.1
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    • pp.1-6
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    • 1996
  • For reducing planting distance in greenhouse grown watermelon(Citrullus vulgaris S.) this experiment was carried out to study the effect of DIF on stem elongation and growth. Day temperatures ranged from $25^{\circ}C$ to 35$^{\circ}C$ : night temperatures ranged from $25^{\circ}C$ to 35$^{\circ}C$ at 5$^{\circ}C$ interval. Stem elongation, leaf size, dry weight and flowering were influence by day and night temperatures. Stem elongation and length of internode decrease with increasing night temperature at same day temperature. The optimums for number of leaves categories was with day at 35$^{\circ}C$, and flower production was the lowest at $25^{\circ}C$. Total leaf area meter was maximized at 35/30(DT/NT), but for size pet one leave was the largest 25/25(DT/NT). Responses of leaf size per leaves were similar to that of internode length, with maximum day and night at $25^{\circ}C$. Total plant dry weight was the highest 35/30 (DT/NT) and minimum occurring at 25/30(DT/NT). The shoot/root ratios of dry weight Increased with day temperature up to 3$0^{\circ}C$ and were the highest with night at $25^{\circ}C$. Chlorophyll contents decreased with decreasing day and night temperature.

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