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Polychlorinated Dibenzo-p-dioxins and Polychlorinated Dibenzofurans in Marine Sediments from Mokpo Coastal Water of Korea

  • Moon, Hyo-Bang;Kang, Sung-Kyung;Seo, Won-Ju;Choi, Min-Kyu;Yu, Jun;Choi, Hee-Gu;Park, Jong-Soo
    • Fisheries and Aquatic Sciences
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    • v.10 no.2
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    • pp.93-101
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    • 2007
  • Concentrations of polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) were determined in marine sediments from Mokpo coastal water of Korea. Total and toxic equivalent (TEQ) concentrations of PCDD/Fs ranged from 19.4 to 175pg/g dry weight and ranged from 0.195 to 2.87 pg WHO-TEQ/g dry weight, respectively. These PCDD/F concentrations were below the safety sediment value (20 pg TEQ/g dry weight) of chronic toxicity. A highly significant correlation between the concentrations of PCDD/Fs and total organic carbon (TOC) in marine sediments was observed. Overall PCDD/F concentrations in the sediments were elevated in rivers and at inner locations close to harbors, indicating that these contaminants derived from local discharges of human activities. PCDD/F concentrations measured in our study were lower than those in several industrialized areas in Korea. Nonparametric multidimensional scaling (MDS) ordination showed that combustion processes from industrial complexes are the major source of PCDD/Fs in Mokpo coastal water.

Qualitative and Quantitative Analysis of Dibenzocyclooctadiene Lignans for the Fruits of Korean "Omija" (Schisandra chinensis) (한국산 오미자로부터 디벤조사이클로옥타디엔 계열 리그난 화합물 정성 및 정량 분석)

  • Kim, Heon Woong;Shin, Jae Hyeong;Lee, Min Ki;Jang, Ga Hee;Lee, Sung Hyeon;Jang, Hwan Hee;Jeong, Seok Tae;Kim, Jung Bong
    • Korean Journal of Medicinal Crop Science
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    • v.23 no.5
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    • pp.385-394
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    • 2015
  • Background : Dibenzocyclooctadiene lignans are secondary metabolites present abundantly in the fruits belonging to the genus Schisandra. According to previous studies, Schisandra lignans exhibit anti-inflammatory, anti-cancer and anti-diabetic properties, as well as an inhibitory effect on platelet aggregation. Therefore, establishing the Korean "Omija" (Schisandra chinensis) as a lignan-rich source, in addition to identifying and quantifying the lignans, is extremely valuable. Methods and Results : Dibenzocyclooctadiene lignans were analyzed with liquid chromatography using diode array detection/mass spectrometry, from methanol extracts subsequently identified by a constructed chemical library of 50 lignans. A total of 27 components of lignan including gomisin S were identified, of which schisandrin, gomisin A, gomisin N, deoxyschisandrin, ${\gamma}$-schisandrin, and schisandrin C were identified as the major components in the Korean Omija, Schisandra chinensis. These compounds were divided into two groups, S-biphenyl and R-biphenyl based on the configurations of the stereoisomers structures with contents of 661.7 and 1350.1mg per 100 g dry weight, respectively. The total lignan content averaged 2011.4mg per 100 g dry weight, of which schisandrin and gomisin N comprised the majority (771.8 and 420.5mg per 100 g dry weight respectively). Conclusions : Lignans which are present in high quantities in the ripe fruit of Schisandra chinensis are important functional compounds that play a major role in the prevention and treatment of human diseases.

Effect of Serpentine as Soil Conditioner on Growth of Turfgrass (토양 개량재로서 사문석이 잔디의 생육에 미치는 영향)

  • 태현숙;고석구;김용선
    • Journal of the Korean Institute of Landscape Architecture
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    • v.30 no.3
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    • pp.86-93
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    • 2002
  • The objectives of this research were to investigate the effect of serpentine as a new soil conditioner for growth of turfgrass. To achieve the goal, pure sand or mixtures of sand and serpentine with various ratios were tested for soil physical properties and the growth effects of perennial ryegrass and zoysiagrass growth were compared. Major results of this research are summarized as follows; 1) Hydraulic conductivity of 10~30% serpentine mixtures were observed within the range of 1010~901mm/h which is good for USGA recommendation. Experimental results of pH and EC for various mixtures indicated that the 10% serpentine mixture was the most suitable for turfgrass growth. 2) Perennial ryegass treated with 10% serpentine mixture showed the highest visual quality(p<0.01) among all treatments. And serpentine treatment was more effective to improve visual quality of perennial ryegrass than that of zoysiagrass. The treatment of 10% serpentine had better visual qualities than that of 20% in both of zoysiagrass and perennial ryegrass. Treatment with the right amount of serpentine extends green period for one to two weeks during early winter in both zoysiagrass and perennial ryegrass. 3) In perennial ryegrass, the treatment of 10% serpentine resulted in an increase of total dry weight compare with those of zeolite or barley stone, and also dramatically promoted the dry weight by 15% compared with sand 100%(control). Total dry weight of zoysiagrass treated with 10% serpentine was 9% higher than that of san. These results indicated that serpentine can be a good soil conditioner for both zoysiagrass and perennial ryegrass when it is blended with sand within a range of 10 to 20% by volume.

A Comparative Study on Digestive Parameters in an Indigenous Fat-tailed and a Conventional Dorper Sheep Fed on Maize Stover Crop Residue

  • Mwenya, B.;Wollny, C.;Blummel, M.;Banda, J.;Takahashi, J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.4
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    • pp.529-533
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    • 2003
  • The intake, dry matter (DM) digestibility, total mean retention time (TMRT) and weight changes were investigated in four fat tailed and four Dorper sheep fed on a maize stover crop residue. Animals were subjected to five dietary treatments, which were: 90, 135 and $180g\;DM/kgBW^{0.75}/day$ of chopped maize stover offered with urea, and in subsequent dietary treatments $180g\;DM/kgBW^{0.75}/day$ of stover supplemented with either dried bean husks or poultry manure. Although not statistically different (p>0.05) fat tailed sheep had higher maize stover intake than the Dorper sheep when expressed per metabolic body weight. The DM intake and digestibility increased significantly (p<0.001) in both sheep breeds when the maize stover offer levels were increased from 90 to $135g/kgBW^{0.75}/day$. Dorper sheep had significantly (p<0.001) higher DM digestibility and longer total mean retention times for both the liquid and solid matter than the fat-tailed sheep. Fat-tailed sheep gained 4.75 kg while Dorper sheep gained 3.75 kg over the entire experimental period. The weight change per dietary treatment was affected by the amount of maize stover and the type and amount of supplement consumed. The higher DM digestibility and longer total mean retention time in Dorper sheep shows that the breed has adapted to utilize the low quality roughage diets. An indigenous fat tailed, has the potential to ingest high volumes of fibrous feed and has higher voluntary intakes relative to its body size. The two sheep breeds are a valuable genetic resource in sub Saharan Africa that is characterized by long dry season with no readily available and affordable conventional feeds for small ruminants.

Studies on Potential utilization of Earthworm Cast as Vegetable Plant Growth Media - Effects of mixing ratios with earthworm cast in nursery soil on pepper(Capsicum annuum L.) seedling growth - (원예용 육모 상토로서의 지렁이 분립의 이용에 관한 연구 - 지령이 분립의 혼합비율이 고추묘의 생육에 미치는 영향 -)

  • Han, Junga;Jun, hajoon;Jo, Ikhwan
    • Journal of the Korea Organic Resources Recycling Association
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    • v.2 no.1
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    • pp.65-73
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    • 1994
  • Earthworm cast was investigated in this study in order to domestically develop inexpensive and safe plug nursery soil. 20,30,40 and 50% of earthworm cast were added to the nursery soil as vegetable plant growth media, in which peatmoss and vermiculite constituted the rest of the soil. The effects of earthworm cast on the growth of pepper (Capsicum annuum L.) seedling were obtained as follows. 1. According to the growth stages, significant differences(p=0.001) were recorded in number of leaves, leaf area and biological dry matter yield per plant(shoor or root dry matter yield per plant). In view of mixing ratios in the nursery soil, number of leaves, leaf area and stem weight per plant showed differences significantly at 1% level and leaf weight, root weight and biological dry weight per plant at 5% level, respectively. 2. The nursery soil with earthworm cast showed increased number of leaves and leaf area per plant compared to the commercial nursery soil particularly in the later stage of this study than in the earlier stage 3. Through the total period of seedling growth, leaf weight, stem weight and root weight per plant in the nursery soil with earthworm cast were genrally higher than those in the control and this trend was apparant in the treatment of more than 40% of mixing ratio with earthworm cast. 4. Although leaf weight per plant was higher than stem weight per plant till the 3rd week, from the 4th week stem weight per plant was getting higher. In the later stage of seedling growth, the stem weight was higher in the earthworm cast mixed treatment than that in the control. 5. There was no significant difference on biological dry matter yield in the earlier stage of this study, however in the later stage, it was higher in the earthworm cast mixed treatment than that in the control.

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Validation of an Analytical Method for Deacetylasperulosidic acid, Total Sugar and Monosaccharide Analysis in Fermented Morinda citrifolia Polysaccharide Powder (발효노니 다당체 분말의 deacetylasperulosidic acid, 총당 및 단당류 분석법 검증)

  • Kwon, Heeyeon;Choi, Jisoo;Kim, Soojin;Kim, Eunmin;Uhm, Jihyun;Kim, Bokyung;Lee, Jaeyeon;Kim, Yongdeok
    • Journal of Food Hygiene and Safety
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    • v.37 no.4
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    • pp.216-224
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    • 2022
  • This study was aimed at validating the analysis methods for deacetylasperulosidic acid (DAA), total sugar, galacturonic acid, glucose, and galactose, which are the indicator components of fermented Morinda citrifolia polysaccharide extract (Vitalbos). We modified the previously reported methods for validating the analytical methods. The specificity, linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ) were measured using phenol-sulfuric acid method and high-performance liquid chromatography (HPLC). The retention time and spectrum of the standard solution of Vitalbos coincided, confirming the specificity. The calibration curve correlation coefficient (R2), of five indicator components, ranged from 0.9995-0.9998, indicating excellent linearity of 0.99 or more. The intra-day and inter-day precision range of the assay was 0.14-3.01%, indicating a precision of less than 5%. The recovery rate was in the range of 95.13-105.59%, presenting excellent accuracy. The LOD ranged from 0.39 to 0.84 ㎍/mL and the LOQ ranged from 1.18 to 2.55 ㎍/mL. Therefore, the analytical method was validated for DAA, total sugar, galacturonic acid, glucose, and galactose, in Vitalbos. The indicator component content in Vitalbos was determined using a validated method. The contents of DAA, total sugar, galacturonic acid, glucose, and galactose were 2.31±0.06, 475.92±5.95, 72.83±1.05, 71.63±2.44, and 67.30±2.31 mg/g of dry weight, respectively. These results suggest that the developed analytical method is efficient and could contribute to the quality control of Vitalbos, as a healthy functional food material.

Prediction of the content of white clover and perennial ryegrass in fresh or dry mixtures made up from pure botanical samples, by near infrared spectroscopy

  • Blanco, Jose A.;Alomar, Daniel J.;Fuchslocher, Rita I.
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1266-1266
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    • 2001
  • Pasture composition, an important attribute determining sward condition and value, is normally assessed by hand separation, drying and measuring weight contribution of each species in the mixture. This is a tedious, time and labour consuming procedure. NIRS has demonstrated the potential for predicting botanical composition of swards, but most of the work has been carried out on dry samples. The aim of this work was to evaluate the feasibility of developing NIR models for predicting the white clover and ryegrass content in fresh or dry mixtures artificially prepared from pure samples of both species. Mixtures from pure stands of white clover(Trifolium repens) and perennial ryegrass (Lolium perenne) were prepared with different proportions (0 to 100%) of each species (fresh weight). A total of 55 samples were made (11 mixtures,5 cuts). Spectra (400 to 2500 nm) were taken from fresh chopped (rectangular cuvettes, transport sample module) samples, in a NIR Systems 6500 scanning monochromator controlled by the software NIRS 3 (Infrasoft International), which was also utilized for calibration development. Different math treatments (derivative order, subtraction gap and smooth segment) and a scatter correction treatment of the spectra (SNV and Detrend) were tested. Equations were developed by modified partial least squares. Prediction accuracy evaluated by cross-validation, showed that percentage of clover or ryegrass, as contribution in dry weight, can be successfully percentage of clover or ryegrass, as contribution in dry weight, can be successfully predicted either on fresh or dried samples, with equations developed by different math treatments. Best equations for fresh samples were developed including a first, second, or third derivative, whereas for dry samples best equations included a second or third derivative. Standard errors of ross validation were about 6% for fresh and 3.6% for dry samples, Coefficient of determination of cross validation (1-VR) were over 0.95 times the value of SECV for fresh samples and over 8 times the value of SECV for dry samples. Scatter correction (SNV and Detrend) in general improved prediction accuracy. It is concluded more precise on dried and ground samples, it can be used with an acceptable error level and less time and labour, on fresh samples.

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Effects of Ensiled Cassava Tops on Rumen Environment Parameters, Thyroid Gland Hormones and Liver Enzymes of Cows Fed Urea-treated Fresh Rice Straw

  • Khang, Duong Nguyen;Wiktorsson, Hans
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.7
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    • pp.936-941
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    • 2004
  • Four rumen-cannulated cows (330 kg average weight at 4 years) were used to evaluate the supplement of ensiled cassava tops (ECT) (variety KM 94, 39% DM) on rumen functions, thyroid hormones and liver enzymes. The treatments, arranged in a 4 $\times$4 Latin square design, were ECT at 0, 50, 100 and 150 g CP 100 kg$^{-1}$ body weight (BW), and a basal diet of urea-treated fresh rice straw (UFRS) ad libitum and 1.1 kg dry matter (DM) cassava root meal (CRM) in each 30 day study period. The results showed a continuous decrease in dry matter intake (DMI) of UFRS with increasing level of ECT supplement (p<0.001). The highest total DMI was observed for treatment ECT$_{150}$ (2.68 kg DM 100 kg$^{-1}$ BW day$^{-1}$) followed by treatments ECT$_{100}$, ECT$_{50}$ and ECT$_{0}$, with 2.47, 2.24 and 2.06 kg DM 100 kg$^{-1}$ BW⋅day$^{-1}$, respectively. Increasing levels of ECT supplement increased the concentration of total volatile fatty acids (p<0.05) and ammonia nitrogen (p<0.05) and resulted in a decrease in pH (p<0.05). Overall average plasma triiodothyronine and thyroxine concentrations were 0.80, 0.82, 0.85 and 0.69 ng ml$^{-1}$ (p>0.05), and 50.9, 49.5, 50.7 and 42.4 ng ml-1 (p>0.05) for treatments ECT$_{0}$, ECT$_{50}$, ECT$_{100}$ and ECT$_{150}$, respectively. There were non-significant differences in alanine aminotransferase and aspartate aminotransferase among treatments. It is concluded that ECT is a valuable protein-rich feed supplement to cattle, and the highest level of on average 2.48 kg DM ECT per cow and day (28% of total DMI) did not significantly affect thyroid gland hormones and liver enzymes in cows.

Growth Response and Arsenic Uptake of White Clover (Trifolium repens) and Evening Primrose(Oenothera odorata) Colonized with Arbuscular Mycorrhizal Fungi in Arsenic-Contaminated Soil

  • Kim, Dae-Yeon;Lee, Yun-Jeong;Lee, Jong-Keun;Koo, Na-Min;Kim, Jeong-Gyu
    • Korean Journal of Environmental Agriculture
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    • v.27 no.1
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    • pp.50-59
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    • 2008
  • A greenhouse experiment was conducted to investigate the role of the arbuscular mycorrhizal(AM) fungus, Glomus mosseae(BEG 107) in enhancing growth and arsenic(As) and phosphorus(P) uptake of white clover(Trifolium repens) and evening primrose(Oenothera odorata) in soil collected from a gold mine having concentrations of 381.6 mg total As $kg^{-1}$ and 20.5 mg available As $kg^{-1}$. Trifolium repens and O. odorata are widely distributed on abandoned metalliferous mines in Korea. The percent root colonization by the AM fungus was 55.9% and 62.3% in T. repens and O. odorata, respectively, whereas no root colonization was detected in control plants grown in a sterile medium. The shoot dry weight of T. repens and O. odorata was increased by 323 and 117% in the AM plants compared to non-mycorrhizal(NAM) plants, respectively. The root dry weight increased up to 24% in T. repens and 70% in O. odorata following AM colonization compared to control plants. Mycorrhizal colonization increased the accumulation of As in the root tissues of T. repens and O. odorata by 99.7 and 91.7% compared to the NAM plants, respectively. The total uptake of P following AM colonization increased by 50% in T. repens and 70% in O. odorata, whereas the P concentration was higher in NAM plants than in the AM plants. Colonization with AM fungi increased the As resistance of the host plants to As toxicity by augmenting the yield of dry matter and increasing the total P uptake. Hence, the application of an AM fungus can effectively improve the phytoremediation capability of T. repens and O. odorata in As-contaminated soil.

Growing Response of Cyrtomium falcatum and Rumohra aristata Indoor as Influenced by Light Quality Treatment (실내에서 광질이 도깨비고비와 가는쇠고사리의 생육에 미치는 영향)

  • Bang, Kwang-Ja;Ju, Jin-Hee;Kwon, Min-Hoon
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.7 no.6
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    • pp.49-53
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    • 2004
  • This study was aimed to promote evergreen ferns native to Korea as a material for interior landscape by investigate effects of light quality on the growth of Cyrtomium falcatum and Rumohra aristata, in an indoor environment that artificial light was used, especially. Result of experiments are as follows; 1. Wavelengths were measured as control(=570~580nm), red(=600~610nm), yellow(=550~580nm), green(=500~510nm) and blue(=430~440nm) between different color film. The order of photon flux density was red>yellow>control>green>blue decreased. 2. Although there was no difference in the growth of Cyrtomium falcatum depending on light quality, in case of fronds with sori and new fronds, there were highest under red film. Fresh weight was no significant in all treatments, but dry weight was increased with green>control>yellow>blue>red in order. 3. In case of Rumohra aristata, there was no difference in its growth, however, number of total fronds was highest under green film. Although fresh weight was increased with yellow film, dry weight was highest under green film.