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Effects of Cultivation Environment and Fruit Ripeness on Microbial Load in Mulberry (재배환경 및 과실 숙도가 오디의 미생물학적 부하량에 미치는 영향)

  • Ryu, Song Hee;Yun, Bohyun;Kim, Hye-Young;Choi, Ah-Hyun;Kim, Se-Ri;Kim, Won-Il;Ryu, Jae-Gee;Han, Sanghyun
    • Journal of Food Hygiene and Safety
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    • v.32 no.1
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    • pp.1-7
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    • 2017
  • This study was conducted to investigate the microbial loads in mulberry fruits depending on cultivation environment and fruit ripeness. The population levels of total aerobic bacteria in mulberry fruits collected from open field orchards were higher than those from three plots protected within plastic green houses. In regards to fruit ripeness, the levels of total aerobic bacteria in ripe black fruits were higher than those in unripe green and red mulberry. From the farms into where livestock animals were allowed to enter, Escherichia coli was detected in soil at a level of 4.26~4.94 log CFU/g and in mulberry fruits at 5.03~6.07 log CFU/g, while no coliform and E. coli were detected from where the intrusion of livestock was prevented. We also examined the density change of inoculated E. coli in mulberry fruits as they were becoming mature. While E. coli did not increase in green fruits, two and four log CFU/g increases at $20^{\circ}C$ and $37^{\circ}C$, respectively, were observed with red and fully mature black mulberries during 48 hours incubation. To ensure the food safety of mulberry, it is suggested that the introduction of E. coli into a farm through livestock should be prevented and more hygienic caution should be taken especially when the fruits are ripe.

Pollutant Contents with Particle Size Distribution in Bridge Road Drainage Sediment (교량도로 배수받이 퇴적물질의 입경별 오염물질 함량)

  • Lee, Jun-Ho;Cho, Yong-Jin;Bang, Ki-Woong
    • Journal of Korean Society of Environmental Engineers
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    • v.29 no.12
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    • pp.1360-1365
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    • 2007
  • The purpose of this study is to present the basic data for nonpoint pollutant loads from bridge road drainage sediments using the results to analyze organic matter and heavy metals from the four bridge drainage sampling sites with sediments of different particle size ranges. The sediment sample was collected from the bridge road drainage and the masses of nine sediments fractions were obtained after drying the separated sediment in an over at $85^{\circ}C:>2,000{\mu}m$, $1,000\sim2,000{\mu}m$, $850\sim1,000{\mu}m$, $425\sim850{\mu}m$, $212\sim425{\mu}m$, $125\sim212{\mu}m$, $90\sim125{\mu}m$, $75\sim90{\mu}m$, $<75{\mu}m$. The sediment extract was analyzed water quality constituents, including chemical oxygen demand(COD), total nitrogen(T-N), total phosphorus(T-P), heavy metals and particle size distribution. The results indicate that most of particle size ranges of the bridge road sediments was $125\sim425{\mu}m$, and portion of $<75{\mu}m$ was low. But most of the pollutants are associated with the finer fractions of the load sediments. As the results of analysis, the range and average values of COD, T-N, T-P, Fe, Cu, Cr, and Pb were $177\sim198.8$ mg/kg(77.6 mg/kg), $23\sim200$ mg/kg(83 mg/kg), T-P $18\sim215$ mg/kg(129 mg/kg), and $1,508\sim5,612$ mg/kg(3,835 mg/kg), $9.2\sim69.3$ mg/kg(49 mg/kg), $19.1\sim662.2$ mg/kg(214 mg/kg), and $28.4\sim251.4$ mg/kg(114 mg/kg), respectively. The relationship between sediment size and pollutants concentration have an inverse proportion. The removal of road sediments with frequently could be reduced the significant nonpoint pollutant load, because of the bridge road sediment contains considerable micro-particles and heavy metals.

Sewer Decontamination Mechanism and Pipe Network Monitoring and Fault Diagnosis of Water Network System Based on System Analysis (시스템 해석에 기초한 하수관망 오염 매카니즘과 관망 모니터링 및 이상진단)

  • Kang, OnYu;Lee, SeungChul;Kim, MinJeong;Yu, SuMin;Yoo, ChangKyoo
    • Korean Chemical Engineering Research
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    • v.50 no.6
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    • pp.980-987
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    • 2012
  • Nonpoint source pollution causes leaks and overtopping, depending on the state of the sewer network as well as aggravates the pollution load of the aqueous water system as it is introduced into the sewer by wash-off. According, the need for efficient sewer monitoring system which can manage the sewage flowrate, water quality, inflow/infiltration and overflow has increased for sewer maintenance and the prevention of environmental pollution. However, the sewer monitoring is not easy since the sewer network is built in underground with the complex nature of its structure and connections. Sewer decontamination mechanism as well as pipe network monitoring and fault diagnosis of water network system on system analysis proposed in this study. First, the pollution removal pattern and behavior of contaminants in the sewer pipe network is analyzed by using sewer process simulation program, stormwater & wastewater management model for expert (XP-SWMM). Second, the sewer network fault diagnosis was performed using the multivariate statistical monitoring to monitor water quality in the sewer and detect the sewer leakage and burst. Sewer decontamination mechanism analysis with static and dynamic state system results showed that loads of total nitrogen (TN) and total phosphorous (TP) during rainfall are greatly increased than non-rainfall, which will aggravate the pollution load of the water system. Accordingly, the sewer outflow in pipe network is analyzed due to the increased flow and inflow of pollutant concentration caused by rainfall. The proposed sewer network monitoring and fault diagnosis technique can be used effectively for the nonpoint source pollution management of the urban watershed as well as continuous monitoring system.

Finite element analysis of peri-implant bone stress influenced by cervical module configuration of endosseous implant (임플란트 경부형상이 주위골 응력에 미치는 영향에 관한 유한요소법적 분석)

  • Chung, Jae-Min;Jo, Kwang-Heon;Lee, Cheong-Hee;Yu, Won-Jae;Lee, Kyu-Bok
    • The Journal of Korean Academy of Prosthodontics
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    • v.47 no.4
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    • pp.394-405
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    • 2009
  • Statement of problem: Crestal bone loss, a common problem associated with dental implant, has been attributed to excessive bone stresses. Design of implant's transgingival (TG) part may affect the crestal bone stresses. Purpose: To investigate if concavely designed geometry at a dental implant's TG part reduces peri-implant bone stresses. Material and methods: A total of five differently configured TG parts were compared. Base model was the ITI one piece implant (Straumann, Waldenburg, Switzerland) characterized by straight TG part. Other 4 experimental models, i.e. Model-1 to Model-4, were designed to have concave TG part. Finite element analyses were carried out using an axisymmetric assumption. A vertical load of 50 N or an oblique load of 50 N acting at $30^{\circ}$ with the implant's long axis was applied. For a systematic stress comparison, a total of 19 reference points were defined on nodal points around the implant. The peak crestal bone stress acting at the intersection of implant and crestal bone was estimated using regression analysis from the stress results obtained at 5 reference points defined along the mid plane of the crestal bone. Results: Base Model with straight configuration at the transgingival part created highest stresses on the crestal bone. Stress level was reduced when concavity was imposed. The greater the concavity and the closer the concavity to the crestal bone level, the less the crestal stresses. Conclusion: The transgingival part of dental implant affect the crestal bone stress. And that concavely designed one may be used to reduce bone stress.

Organic Matter and Heavy Metals Pollution Assessment of Surface Sediment from a Fish Farming Area in Tongyoung-Geoje Coast of Korea (통영-거제 연안 어류 양식장 표층 퇴적물 중 유기물 및 중금속 오염 평가)

  • Hwang, Dong-Woon;Hwang, Hyunjin;Lee, Garam;Kim, Sunyoung;Park, Sohyun;Yoon, Sang-Pil
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.27 no.4
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    • pp.510-520
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    • 2021
  • To understand the status of organic matter and heavy metal pollution in surface sediment of a fish farming area, we have measured the concentrations of total organic carbon (TOC), total nitrogen (TN), and heavy metals (As, Cd, Cr, Cu, Fe, Hg, Mn, Pb, and Zn) in surface sediments of a fish farming area near Tongyoung-Geoje coast. The mean concentrations of TOC and TN were 22.7 mg/g and 3.4 mg/g, respectively, and were much higher than those in surface sediments of a semi-enclosed bay in the southern coast of Korea. The mean concentrations of As, Cd, Cr, Cu, Fe, Hg, Mn, Pb, and Zn were 10.5 mg/kg, 0.37 mg/kg, 82.9 mg/kg, 127 mg/kg, 4.19%, 0.041 mg/kg, 596 mg/kg, 39.5 mg/kg, and 175 mg/kg, respectively, and the mean concentrations of Cd and Cu were three times higher than those in surface sediments of shellfish farming area in the southeastern coast of Korea. In addition, the concentrations of TOC and corrected Cu exceeded the values of sediment quality guidelines applied in Korea, and pollution load index (PLI) and ecological risk index (ERI) showed that the metal concentrations in the sediments of some fish farming area have a strongly negative ecological impact on benthic organisms, although most metal concentrations did not exceed the sediment quality guidelines. Based on overall assessment results, the surface sediments of fish farming areas in the study region are polluted with organic matter and some heavy metals. Thus, a comprehensive management plan is necessary to improve the sedimentary environments, identify primary contamination sources, and reduce the input of pollution load for organic matter and heavy metals in the sediments of fish farming areas.

Influence of Land Use on the Pollution Load in the Saemangeum Basin (새만금 유역에서 토지 이용이 오염부하에 미치는 영향 평가)

  • Lee, Deog-Bae;Kim, Jong-Cheon;Lee, Kyung-Bo;Kim, Jong-Gu;Park, Chan-Won
    • Korean Journal of Soil Science and Fertilizer
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    • v.39 no.4
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    • pp.237-244
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    • 2006
  • The SMG project has been driven to secure food and water resources by closing of the SMG dyke for the preparation of the unification of Korean peninsular. It was investigated for pollution loads, land use distribution and water consumption for environmental assessments in two watersheds, the Mankyeong River (MK) and the Dongjin River (DJ) to assess the role of agricultural land on the alleviation of pollution loads to the SMG basin. It is needed to give the priority in managing pollution sources to conserve freshwater in the Saemangeum (SMG) basin after the completion of the SMG reclamation from tideland. The MK has $700million\;m^3$ water of which 14.1% were used for living, 73.6% for agriculture and 12.3% for industry. The DJ has $505million\;m^3$ water of which 3.0% for living, 94.5% for agriculture and 2.5% for industry. As compared to proportion of each land of total area, agricultural land was 1.4 times larger, livestock farming 7 times larger, forest 0.74 times smaller, and built-up area 0.67 times smaller in DJ watershed than in MK watershed. Pollution sources in MK and DJ watersheds were originated at a higher proportion from population including the sewage disposal and a livestock farming area rather than from the land. Water consumption and land use distribution influenced the water quality of the rivers; DJ watershed had far lower value of electric conductivity, $BOD_5$, TN and TP than MK watershed. A large proportion of paddy field also influenced to reduce pollute loadings after rainfall; DJ watershed, which has a relatively large area of paddy fields, had a far lower delivery load after rainfall than MK watershed even though DJ watershed had large livestock farming area. As paddy fields was irrigated by Iksancheon water, 37% of nitrogen, 50% of phosphates and 14.0% of $BOD_5$ was removed by the paddy field just after flowing 150 meter, and rice plants could remove TN 100.0 kg, $P_2O_5$ 24.0 kg, and $K_2O$ 119.2 kg per hectare at harvest by irrigation of Iksancheon water. Conclusively, rice paddy fields played a positive role to conserve the water quality in the Iksancheon watershed.

Study of system using load cell for real time weight sensing of artificial incubator (인공부화기의 실시간 중량감지를 위한 로드셀을 이용한 시스템 연구)

  • jeong, Jin-hyoung;Kim, Ae-kyung;Lee, Sang-Sik
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.11 no.2
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    • pp.144-149
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    • 2018
  • The eggs are incubated for 18 days through the generator and incubated in the developing incubator. During the developmental period, the weight loss of the fetus is correlated with the ventricular formation, and the proper ventricular formation is also associated with the healthy embryonic hatching and the egg hatching rate. However, in the incubator period of the domestic hatchery, it is a reality to acquire the resultant side by the Iranian standard weight measurement with the experience of the hatchery and the person concerned and the development period without the apparatus for measuring the present weight. As a result, prevalence of early mortality, hunger and illness during hatching are frequent. Monitoring the reduction of weaning weight is crucial to obtaining chick quality and hatching performance with weight changes within the development machine. Water loss is different depending on the size of eggs, egg shell, and elder group. We can expect to increase the hatching rate by measuring the weight change in real time and optimizing the ventilation change accordingly. There is a need to develop a real-time measurement system that can control 10 to 13% reduction of the total weight during hatching. The system through this study is a way to check the one - time directly when moving the existing egg, and it is impossible to control the measurement of the fetal water evaporation within the development period. Unlike systems that do not affect the hatching rate, four load cells are connected in parallel on the Arduino sketch board and the AT-command command is used to connect the mobile phone and computer in real time. The communication speed of Bluetooth was set to 15200 to match the communication speed of Arduino and Hyper-terminal program. The real - time monitoring system was designed to visually check the change of the weight of the fetus in the artificial incubator. In this way, we aimed to improve the hatching rate and health condition of the hatching eggs.

A study on drainage characteristics and load amount evaluation by crop type in a hydroponic cultivation facility of horticultural complex (수경재배 시설원예단지 작물 유형별 배액 특성 및 부하량 평가 연구)

  • Jin, Yujeong;Kang, Taegyoung;Lim, Ryugab;Kim, Hyunwoo;Kang, Donghyeon;Park, Minjung;Son, Jinkwan
    • Journal of Wetlands Research
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    • v.23 no.4
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    • pp.352-363
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    • 2021
  • The purpose of this study was to evaluate the load of nutrients contained in the drainage discharged from the facility horticultural complex and to use them for re-use of fluids and design for introduction of water treatment plants. Representative hydroponic cultivation crops were selected as tomato, paprika, cucumber, and strawberry, and the total number of samples analyzed for water quality was 80. As a result of the analysis, since various fertilizer components such as N, P, K+, Na+, Mg2+, Ca2+, Si4+, HCO3-, Cl-, S2-, Fe, Mn, Cu, Zn, Mo and B are contained at very high concentrations in the drainage, the need for water treatment was confirmed. Through statistical analysis, it was analyzed that the drainage concentration of strawberries was lower than that of tomatoes, paprika, and cucumbers. In the case of tomatoes, these essential ion concentrations are the highest, so it was confirmed that they are subject to valuable resources in terms of reuse of fertilizers. The load of N and P of the drainage discharged from the facility horticultural complex 1m2 was analyzed. For N, the daily processing capacity of 4.0 kg of tomatoes, 3.3 kg of paprika, 3.0 kg of cucumbers, and 1.5 kg of strawberries was calculated based on 1 ha. It was suggested that the P concentration needs a scale and capacity that can handle 0.5 kg of tomatoes, 0.6 kg of paprika, 0.4 kg of cucumber, and 0.2 kg of strawberries per day. Through this study, the amount of nitrogen and phosphorus contained in the drainage discharged from the greenhouse of each crop was evaluated to analyze the economy. In addition, it was expected to be used as basic data that can be used to calculate the treatment capacity to be reflected when introducing water treatment facilities in facility horticultural complexes for sustainable agriculture.

Development of Summer Leaf Vegetable Crop Energy Model for Rooftop Greenhouse (옥상온실에서의 여름철 엽채류 작물에너지 교환 모델 개발)

  • Cho, Jeong-Hwa;Lee, In-Bok;Lee, Sang-Yeon;Kim, Jun-Gyu;Decano, Cristina;Choi, Young-Bae;Lee, Min-Hyung;Jeong, Hyo-Hyeog;Jeong, Deuk-Young
    • Journal of Bio-Environment Control
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    • v.31 no.3
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    • pp.246-254
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    • 2022
  • Domestic facility agriculture grows rapidly, such as modernization and large-scale. And the production scale increases significantly compared to the area, accounting for about 60% of the total agricultural production. Greenhouses require energy input to create an appropriate environment for stable mass production throughout the year, but the energy load per unit area is large because of low insulation properties. Through the rooftop greenhouse, one of the types of urban agriculture, energy that is not discarded or utilized in the building can be used in the rooftop greenhouse. And the cooling and heating load of the building can be reduced through optimal greenhouse operation. Dynamic energy analysis for various environmental conditions should be preceded for efficient operation of rooftop greenhouses, and about 40% of the solar energy introduced in the greenhouse is energy exchange for crops, so it should be considered essential. A major analysis is needed for each sensible heat and latent heat load by leaf surface temperature and evapotranspiration, dominant in energy flow. Therefore, an experiment was conducted in a rooftop greenhouse located at the Korea Institute of Machinery and Materials to analyze the energy exchange according to the growth stage of crops. A micro-meteorological and nutrient solution environment and growth survey were conducted around the crops. Finally, a regression model of leaf temperature and evapotranspiration according to the growth stage of leafy vegetables was developed, and using this, the dynamic energy model of the rooftop greenhouse considering heat transfer between crops and the surrounding air can be analyzed.

Viral Load Dynamics After Symptomatic COVID-19 in Children With Underlying Malignancies During the Omicron Wave

  • Ye Ji Kim;Hyun Mi Kang;In Young Yoo;Jae Won Yoo;Seong Koo Kim;Jae Wook Lee;Dong Gun Lee;Nack-Gyun Chung;Yeon-Joon Park;Dae Chul Jeong;Bin Cho
    • Pediatric Infection and Vaccine
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    • v.30 no.2
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    • pp.73-83
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    • 2023
  • Purpose: This study aimed to investigate the viral load dynamics in children with underlying malignancies diagnosed with symptomatic coronavirus disease 2019 (COVID-19). Methods: This was a retrospective longitudinal cohort study of patients <19 years old with underlying hemato-oncologic malignancies that were diagnosed with their first symptomatic severe acute respiratory syndrome coronavirus 2 polymerase chain reaction (PCR)-confirmed COVID-19 infection during March 1 to August 30, 2022. Review of electronic medical records and telephone surveys were undertaken to assess the clinical presentations and transmission route of the patients. Thresholds of negligible likelihood of infectious virus was defined as E gene reverse transcription (RT)-PCR cycle threshold (Ct) value ≥25. Results: During the 6-month study period, a total of 43 children with 44 episodes of COVID-19 were included. Of the 44 episodes, the median age of the patients included was 8 years old (interquartile range [IQR], 4.9-10.5), and the most common underlying disease was acute lymphoid leukemia (n=30, 68.2%), followed by patients post-hematopoietic stem cell transplantation (n=8, 18.2%). Majority of the patients had mild COVID-19 (n=32, 72.7%), and three patients (7.0%) had severe/critical COVID-19. Furthermore, 2.3% (n=1) died of COVID-19 associated acute respiratory distress syndrome. The largest percentage of the patients showed E gene RT-PCR Ct value ≥25 between 15-21 days (n=13, 39.4%), followed by 22-28 days (n=10, 30.3%). In 15.2% (n=5), E gene RT-PCR Ct value remained <25 beyond 28 days after initial positive PCR. Refractory malignancy status (β, 67.0; 95% confidence interval, 7.0-17.0; P=0.030) was significantly associated with prolonged duration of E gene RT-PCR <25. A patient with prolonged duration of E gene RT-PCR Ct value <25 was suspected to have infectivity shown by the transmission of the virus to his mother at day 86 after his initial positive test. Conclusions: Children that acquire symptomatic COVID-19 during refractory malignancy state are at a high risk for prolonged shedding warranting PCR-based transmission precautions in this cohort of patients.