• Applied Biological Chemistry
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• 제40권2호
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• pp.163-166
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• 1997

효모(Torulopsis bombicola)를 콩기름으로 강화한 배지에서 $25^{\circ}C$로 7일간 진탕배양하였다. 얻어진 배양액으로부터 sophorose-lipid를 분리하였고, 이를 알칼리처리하여 sophorose를 제조하였다. 또한 배양액중의 sophorose를 아세칠화한 후 silica gel column chromatography하여 아세칠화물을 분리하였고, 이를 실온에서 알칼리 처리하여 sophorose를 제조하였다. 한편, 회화나무(Sophora japonica)의 미숙과실의 MeOH 추출물을 용매분획한 후, 얻어진 n-BuOH 분획을 silica gel column chromatography하여 플라보노이드 배당체 분획을 분리하였고, 이를 $0.02\;N{\;}H_2SO_4$로 가수분해하여 sophorose를 제조하는 방법을 확립하였다.

• 한국응용과학기술학회지
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• 제17권4호
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• pp.213-225
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• 2000

Sophorolipids were biosynthesized using a strain of yeast, Torulopsis bombicola ATCC22214. It has been reported that this yeast gives the highest yields for the production of biosurfactant sophorolipids. Hence, this yeast was used in this study. One of the objectives of this study is to increase the yield of the sophorolipid synthesis. To meet this end, basic culture medium was formulated on the basis of literature research to-date. When this medium was used, the increase in yield from 15% to 150% was observed compared to using the media in the literature. To examine how the interfacial characteristics of sophorolipids change with substrate, glucose (the first carbon source) was maintained in the media and after being cultured for three days, the second carbon sources such as alkanes, vegetable oils, alcohols or organic acids were added. The whole broth was extracted twice with ethyl acetate and the extract was analyzed by thin layer chromatograhy(TLC). After qualitative analyses by TLC, surface tensions of sophorolipids were measured by the Wilhelmy plate method and critical micelle concentration(CMC) was determined using these surface tension data. Also, interfacial tensions were measured by the spinning drop method and emulsions of the three-component water/decane/sophorolipid system were tested. Sophorolipids were effective and efficient in terms of surface tension reduction and CMC, but they were ineffective as emulsifiers because emulsions were separated within 30 minutes.