• 한국응용과학기술학회지
• /
• 제38권3호
• /
• pp.801-812
• /
• 2021

바이오틴을 피부에 전달하기 위해 고압균질기를 사용하여 바이오틴과 세라마이드를 모두 함유하는 에토좀에 대하여 연구하였다. 바이오틴이 주된 성분으로 사용되었으며, 세라마이드 NP는 인지질 이중층의 지지체로 활용되었다. 바이오틴은 수용성 내부에 포획되었고, 세라마이드 NP는 에토좀의 이중층에 흡착되었다. 세라마이드 NP를 함유한 에토좀의 물성을 살펴보면 소포체의 크기는 80~130 nm, 다분산지수는 0.09~0.16, 제타 전위는 -40~-49 mV로 측정되었다. 세라마이드 NP가 없는 소포체의 크기는 124.80±1.46 nm, 다분산지수와 제타전위는 각각 0.088±0.018과 -45.48±1.27 mV이었다. 따라서 세라마이드 NP가 함유된 에토좀은 세라마이드 NP가 없는 에토좀에 비해 소포체의 물리적 특성이 개선됨을 알 수 있었다. 세라마이드 NP를 함유한 에토좀의 피부흡수율은 12시간 후 6.13~14.98%이었으며, 반면에 세라마이드 NP가 없는 에토좀의 피부흡수율은 7.08%이었다. 결론적으로 세라마이드 NP를 함유한 에토좀은 피부흡수 효율을 향상시킬뿐만 아니라 소포체의 안정성에도 긍정적인 영향을 미쳤다.

• Journal of Ginseng Research
• /
• 제45권1호
• /
• pp.183-190
• /
• 2021

Background: The circadian rhythm is the internal clock that controls sleep-wake cycles, metabolism, cognition, and several processes in the body, and its disruption has been associated with aging. The differentiated embryo chondrocyte (Dec) gene is related to circadian rhythm. To our knowledge, there are no reports of the relationship between dec gene expression and KRG effect. Therefore, we treated Dec gene knockout (KO) aging mice with KRG to study anti-aging related effects and possible mechanisms. Methods: We evaluated KRG and expression of Dec genes in an ototoxicity model. Dec genes expression in livers of aging mice was further analyzed. Then, we assessed the effects of DEC KO on hearing function in mice by ABR. Finally, we performed DNA microarray to identify KRG-related gene expression changes in mouse liver and assessed the results using KEGG analysis. Results: KRG decreased the expression of Dec genes in ototoxicity model, which may contribute to its anti-aging efficacy. Moreover, KRG suppressed Dec genes expression in liver of wild type indicating inhibition of senescence. ABR test indicated that KRG improved auditory function in aging mouse, demonstrating KRG efficacy on aging related diseases. Conclusion: Finally, in KEGG analysis of 238 genes that were activated and 158 that were inhibited by KRG in DEC KO mice, activated genes were involved in proliferation signaling, mineral absorption, and PPAR signaling whereas the inhibited genes were involved in arachidonic acid metabolism and peroxisomes. Our data indicate that inhibition of senescence-related Dec genes may explain the anti-aging efficacy of KRG.

• Parasites, Hosts and Diseases
• /
• 제58권5호
• /
• pp.551-558
• /
• 2020

The flaviviruses are small single-stranded RNA viruses that are typically transmitted by mosquitoes or tick vectors and are etiological agents of acute zoonotic infections. The viruses are found around the world and account for significant cases of human diseases. We investigated population of culicine mosquitoes in central region of Korean Peninsula, Incheon Metropolitan City and Hwaseong-si. Aedes vexans nipponii was the most frequently collected mosquitoes (56.5%), followed by Ochlerotatus dorsalis (23.6%), Anopheles spp. (10.9%), and Culex pipiens complex (5.9%). In rural regions of Hwaseong, Aedes vexans nipponii was the highest population (62.9%), followed by Ochlerotatus dorsalis (23.9%) and Anopheles spp. (12.0%). In another rural region of Incheon (habitat of migratory birds), Culex pipiens complex was the highest population (31.4%), followed by Ochlerotatus dorsalis (30.5%), and Aedes vexans vexans (27.5%). Culex pipiens complex was the predominant species in the urban region (84.7%). Culicine mosquitoes were identified at the species level, pooled up to 30 mosquitoes each, and tested for flaviviral RNA using the SYBR Green-based RT-PCR and confirmed by cDNA sequencing. Three of the assayed 2,683 pools (989 pools without Anopheles spp.) were positive for Culex flaviviruses, an insect-specific virus, from Culex pipiens pallens collected at the habitats for migratory birds in Incheon. The maximum likelihood estimation (the estimated number) for Culex pipiens pallens positive for Culex flavivirus was 25. Although viruses responsible for mosquito-borne diseases were not identified, we encourage intensified monitoring and long-term surveillance of both vector and viruses in the interest of global public health.

• The Korean Journal of Pain
• /
• 제34권2호
• /
• pp.210-216
• /
• 2021

Background: Postherpetic neuralgia (PHN) is the most common complication of acute herpes zoster. The treatment of PHN remains a challenge for clinical pain management. Despite the effectiveness of anticonvulsants, antidepressants, and lidocaine patches in reducing PHN, many patients still face intractable pain disorders. In this randomized controlled study, we evaluated whether hydromorphone through intravenous patient-controlled analgesia (IV PCA) was effective in relieving PHN. Methods: Patients with PHN were randomly divided into two groups, one group received oral pregabalin with IV normal saline, another group received oral pregabalin with additional IV PCA hydromorphone for two weeks. Efficacy was evaluated at 1, 4, and 12 weeks after the end of the treatments. Results: Two hundred and one patients were followed up for 12 weeks. After treatment, numerical rating scale (NRS) score of patients in the hydromorphone group was significantly lower than that of the control group, and the difference of NRS scores between the two groups was statistically significant at 4 and 12 weeks after treatment. The frequency of breakthrough pain in the hydromorphone group was significantly lower than that in the control group 1 and 4 weeks after treatment. After treatment, the quality of sleep in the hydromorphone group was significantly improved compared with the control group. The most common adverse reactions in the hydromorphone group were dizziness and nausea, with no significant respiratory depression. Conclusions: IV PCA hydromorphone combined with oral pregabalin provides superior pain relief in patients with PHN, which is worthy of clinical application and promotion.

• Fisheries and Aquatic Sciences
• /
• 제24권11호
• /
• pp.360-369
• /
• 2021

Paralytic shellfish toxins (PSTs) and tetrodotoxin (TTX) are neurotoxins that display pharmacological activity that is similar to that of specific sodium channel blockers; they are the principle toxins involved in shellfish and puffer fish poisoning. In Korea, puffer fish is a very popular seafood, and several cases of accidental poisoning by TTX have been reported. Therefore, it is necessary to determine whether puffer fish poisoning incidents are caused by PSTs or by TTX. In this study, we used mouse bioassay (MBA) and liquid chromatograph-tandem mass spectrometry (LC-MS/MS) to determine the presence of PSTs and TTX in puffer fish from an area near Mireuk-do, Tong-Yeong on the southern coast of Korea from January through March, 2014. The toxicity of PSTs and TTX extracts prepared from three organs of each specimen was analyzed by MBA. Most of the extracts killed mice with typical signs of TTX and PSTs. The LC-MS/MS analysis of seven specimens of Takifugu pardalis and Takifugu niphobles, each divided into muscles, intestines, and liver, were examined for TTX. In T. pardalis, the TTX levels were within the range of 1.3-1.6 ㎍/g in the muscles, 18.8-49.8 ㎍/g in the intestines, and 23.3-96.8 ㎍/g in the liver. In T. niphobles, the TTX levels were within the range of 2.0-4.5 ㎍/g in the muscles, 23.9-71.5 ㎍/g in the intestines, and 28.1-114.8 ㎍/g in the liver. Additionally, the toxicity profile of the detected PSTs revealed that dcGTX3 was the major component in T. pardalis and T. niphobles. When PSTs were calculated as saxitoxin equivalents the levels were all less than 0.5 ㎍/g, which is below the permitted maximum standard of 0.8 ㎍/g. These findings indicate that the toxicity of T. pardalis and T. niphobles from the southern coast of Korea is due mainly to TTX and that PSTs do not exert an effect.

• Nuclear Engineering and Technology
• /
• 제54권7호
• /
• pp.2728-2735
• /
• 2022

The research on the flow and heat transfer characteristics of lead bismuth(LBE) is significant for the thermal-hydraulic calculation, safety analysis and practical application of lead-based fast reactors(LFR). In this paper, a new CFD model is proposed to solve the thermal-hydraulic analysis of LBE. The model includes two parts: turbulent model and turbulent Prandtl, which are the important factors for LBE. In order to find the best model, the experiment data and design of 19-pin hexagonal rod bundle with spacer grid, undertaken at the Karlsruhe Liquid Metal Laboratory (KALLA) are used for CFD calculation. Furthermore, the turbulent model includes SST k - 𝜔 and k - 𝜀; the turbulent Prandtl includes Cheng-Tak and constant (Pr_t =1.5,2.0,2.5,3.0). Among them, the combination between SST k - 𝜔 and Cheng-Tak is more suitable for the experiment. But in the low Pe region, the deviation between the experiment data and CFD result is too much. The reason may be the inlet-effect and when Pe is in a low level, the number of molecular thermal diffusion occupies an absolute advantage, and the buoyancy will enhance. In order to test and verify versatility of the model, the NCCL performed by the Nuclear Thermal-hydraulic Laboratory (Nuthel) of Xi'an Jiao tong University is used for CFD to calculate. This paper provides two verification examples for the new universal model.

• 사물인터넷융복합논문지
• /
• 제8권5호
• /
• pp.85-91
• /
• 2022

과학기술과 감성의 융합을 가장 강조하는 IOT 사회환경에서 본 연구는 디자인 분야를 전공하고 있는 중국 유학생 대학원생들의 학습공동체 운영을 기반으로, 디자인 융합 수업에 참여하면서 어떠한 경험을 하였는지 그리고 그 경험의 의미는 무엇인지를 탐색해보는 것을 연구목적으로 하였다. 이를 위해 T대학에 재학하고 있는 12명의 대학원생을 대상으로 디자인 기반의 탐구 방법을 활용하여 다양한 연구 문제를 시각화하는 것으로 실행연구를 하였다. 디자인 중심의 융합수업은 외부환경에 의해 주어지는 표현기법과 연구 참여자들의 내적 동기에 의해 시각적 전략으로 표현되는 내용을 바탕으로 소그룹으로 진행되었다. 연구 참여자들은 연구 문제의 관점과 의도를 연구물에 나타내기 위해 연구 과정에서 시각 자료의 은유적 사용과 분석, 연구 문제에 대한 감각적 접근 등의 다양한 시각적 전략을 사용하여 표현한 융합디자인 연구물은 창의적 사고과정을 표출하는 형식으로 제시되었다. 탐구의 한 방법으로 다양한 경험을 토대로 결과물을 제시한 수업방식은 새로운 수업 형식의 변화 및 교수자들의 실천적 지식 나눔과 학습참여자들의 공동체 참여의 의미를 시사한다고 여긴다.

• Mycobiology
• /
• 제50권1호
• /
• pp.66-78
• /
• 2022

The identification of oleaginous yeast species capable of simultaneously utilizing xylose and glucose as substrates to generate value-added biological products is an area of key economic interest. We have previously demonstrated that the Cutaneotrichosporon dermatis NICC30027 yeast strain is capable of simultaneously assimilating both xylose and glucose, resulting in considerable lipid accumulation. However, as no high-quality genome sequencing data or associated annotations for this strain are available at present, it remains challenging to study the metabolic mechanisms underlying this phenotype. Herein, we report a 39,305,439 bp draft genome assembly for C. dermatis NICC30027 comprised of 37 scaffolds, with 60.15% GC content. Within this genome, we identified 524 tRNAs, 142 sRNAs, 53 miRNAs, 28 snRNAs, and eight rRNA clusters. Moreover, repeat sequences totaling 1,032,129 bp in length were identified (2.63% of the genome), as were 14,238 unigenes that were 1,789.35 bp in length on average (64.82% of the genome). The NCBI non-redundant protein sequences (NR) database was employed to successfully annotate 11,795 of these unigenes, while 3,621 and 11,902 were annotated with the Swiss-Prot and TrEMBL databases, respectively. Unigenes were additionally subjected to pathway enrichment analyses using the Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Cluster of Orthologous Groups of proteins (COG), Clusters of orthologous groups for eukaryotic complete genomes (KOG), and Non-supervised Orthologous Groups (eggNOG) databases. Together, these results provide a foundation for future studies aimed at clarifying the mechanistic basis for the ability of C. dermatis NICC30027 to simultaneously utilize glucose and xylose to synthesize lipids.

• Journal of Microbiology and Biotechnology
• /
• 제32권8호
• /
• pp.1064-1071
• /
• 2022

Arabinogalactans have diverse biological properties and can be used as pharmaceutical agents. Most arabinogalactans are composed of β-(1→3)-galactan, so it is particularly important to identify β-1,3-galactanases that can selectively degrade them. In this study, a novel exo-β-1,3-galactanase, named PoGal3, was screened from Penicillium oxalicum sp. 68, and hetero-expressed in P. pastoris GS115 as a soluble protein. PoGal3 belongs to glycoside hydrolase family 43 (GH43) and has a 1,356-bp gene length that encodes 451 amino acids residues. To study the enzymatic properties and substrate selectivity of PoGal3, β-1,3-galactan (AG-P-I) from larch wood arabinogalactan (LWAG) was prepared and characterized by HPLC and NMR. Using AG-P-I as substrate, purified PoGal3 exhibited an optimal pH of 5.0 and temperature of 40℃. We also discovered that Zn²⁺ had the strongest promoting effect on enzyme activity, increasing it by 28.6%. Substrate specificity suggests that PoGal3 functions as an exo-β-1,3-galactanase, with its greatest catalytic activity observed on AG-P-I. Hydrolytic products of AG-P-I are mainly composed of galactose and β-1,6-galactobiose. In addition, PoGal3 can catalyze hydrolysis of LWAG to produce galacto-oligomers. PoGal3 is the first enzyme identified as an exo-β-1,3-galactanase that can be used in building glycan blocks of crucial glycoconjugates to assess their biological functions.

• 한국콘텐츠학회논문지
• /
• 제21권12호
• /
• pp.66-75
• /
• 2021

우리나라에서 교통 전문 방송사는 서울시에서 운영하는 TBS와 도로교통공단에서 운영하는 TBN 두 군데이다. 본 연구는 두 군데 교통방송 유튜브 플랫폼 활용 현황과 공급되는 콘텐츠의 성격, 활용과정에서 나타난 문제점 및 개선방안을 살펴보는 것을 목적으로 한다. 먼저 유튜브 플랫폼 활용을 보면, TBS의 경우 2021년 3월 전담조직인 '스트리트31팀' 결성하여 총 10명으로 팀을 구성하였으며, TBN은 유튜브 전담조직은 따로 없이 4명의 TF팀 구성하여 운영하고 있다. TBS가 13개 유튜브 채널 중 구독자 110만 명을 보유한 '시민의 방송'을 비롯해 TBS FM(31만 명), TBS eFM(13만 명), 팩트iN스타(32만 명) 등이 주력 채널로 운영하고 있고, <골방 라이브>와 <짤짤이쇼> 등 오리지널 콘텐츠까지 제작하고 있다. 이에 비해 TBN은 메인인 'TBN 통' 구독자가 만 5천 명에 불과하고 12개 지역 네트워크의 유튜브 채널도 부산과 경인을 제외하고 천명도 안 되는 구독자를 보유하고 있다. 오리지널 콘텐츠는 <카린이/ 알려줄car>가 유일하지만 교훈적인 내용으로 별다른 주목을 받지 못하고 있다. 두 방송사 모두 직업의 안정성 때문에 구성원들의 관심 부족과 뉴미디어 콘텐츠 제작에 대해 소극적이다. 따라서 이들 두 방송사에 대한 유튜브 플랫폼 발전을 위해서는 내부의 조직변화 및 구성원들의 인식변화가 필요하고 라이브방송 활성화와 스타 크리에이터의 발굴이 요구되며, 스마트 미디어 환경에 걸맞은 콘텐츠 유통전략을 수립해야 한다.