Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Monitoring Culicine Mosquitoes (Diptera: Culicidae) as a Vector of Flavivirus in Incheon Metropolitan City and Hwaseong-Si, Gyeonggi-Do, Korea, during 2019

  • Bahk, Young Yil (Department of Biotechnology, College of Biomedical and Health Science, Konkuk University) ;
  • Park, Seo Hye (Department of Parasitology and Tropical Medicine & Global Resource Bank of Parasitic Protozoa Pathogens, Inha University School of Medicine) ;
  • Kim-Jeon, Myung-Deok (Department of Infectious Diseases Diagnosis, Incheon Metropolitan City Institute of Public Health and Environment) ;
  • Oh, Sung-Suck (Department of Infectious Diseases Diagnosis, Incheon Metropolitan City Institute of Public Health and Environment) ;
  • Jung, Haneul (Department of Parasitology and Tropical Medicine & Global Resource Bank of Parasitic Protozoa Pathogens, Inha University School of Medicine) ;
  • Jun, Hojong (Department of Parasitology and Tropical Medicine & Global Resource Bank of Parasitic Protozoa Pathogens, Inha University School of Medicine) ;
  • Kim, Kyung-Ae (Department of Infectious Diseases Diagnosis, Incheon Metropolitan City Institute of Public Health and Environment) ;
  • Park, Jong Myong (Department of Infectious Diseases Diagnosis, Incheon Metropolitan City Institute of Public Health and Environment) ;
  • Ahn, Seong Kyu (Department of Parasitology and Tropical Medicine & Global Resource Bank of Parasitic Protozoa Pathogens, Inha University School of Medicine) ;
  • Lee, Jinyoung (Department of Parasitology and Tropical Medicine & Global Resource Bank of Parasitic Protozoa Pathogens, Inha University School of Medicine) ;
  • Choi, Eun-Jeong (Department of Infectious Diseases Diagnosis, Incheon Metropolitan City Institute of Public Health and Environment) ;
  • Moon, Bag-Sou (Department of Infectious Diseases Diagnosis, Incheon Metropolitan City Institute of Public Health and Environment) ;
  • Gong, Young Woo (Department of Infectious Diseases Diagnosis, Incheon Metropolitan City Institute of Public Health and Environment) ;
  • Kwon, Mun Ju (Department of Infectious Diseases Diagnosis, Incheon Metropolitan City Institute of Public Health and Environment) ;
  • Kim, Tong-Soo (Department of Parasitology and Tropical Medicine & Global Resource Bank of Parasitic Protozoa Pathogens, Inha University School of Medicine)
  • Received : 2020.06.29
  • Accepted : 2020.09.07
  • Published : 2020.10.31
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Abstract

The flaviviruses are small single-stranded RNA viruses that are typically transmitted by mosquitoes or tick vectors and are etiological agents of acute zoonotic infections. The viruses are found around the world and account for significant cases of human diseases. We investigated population of culicine mosquitoes in central region of Korean Peninsula, Incheon Metropolitan City and Hwaseong-si. Aedes vexans nipponii was the most frequently collected mosquitoes (56.5%), followed by Ochlerotatus dorsalis (23.6%), Anopheles spp. (10.9%), and Culex pipiens complex (5.9%). In rural regions of Hwaseong, Aedes vexans nipponii was the highest population (62.9%), followed by Ochlerotatus dorsalis (23.9%) and Anopheles spp. (12.0%). In another rural region of Incheon (habitat of migratory birds), Culex pipiens complex was the highest population (31.4%), followed by Ochlerotatus dorsalis (30.5%), and Aedes vexans vexans (27.5%). Culex pipiens complex was the predominant species in the urban region (84.7%). Culicine mosquitoes were identified at the species level, pooled up to 30 mosquitoes each, and tested for flaviviral RNA using the SYBR Green-based RT-PCR and confirmed by cDNA sequencing. Three of the assayed 2,683 pools (989 pools without Anopheles spp.) were positive for Culex flaviviruses, an insect-specific virus, from Culex pipiens pallens collected at the habitats for migratory birds in Incheon. The maximum likelihood estimation (the estimated number) for Culex pipiens pallens positive for Culex flavivirus was 25. Although viruses responsible for mosquito-borne diseases were not identified, we encourage intensified monitoring and long-term surveillance of both vector and viruses in the interest of global public health.

Keywords

References

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