Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Skin Absorption and Physical Property of Ceramide-added Ethosome

세라마이드 함유 에토좀의 물성과 피부흡수

  • Hyeon, Tong-Il (Department of Chemistry & Cosmetics, Jeju National University) ;
  • Yoon, Kyung-Sup (Department of Chemistry & Cosmetics, Jeju National University)
  • 현통일 (제주대학교 화학.코스메틱스학과) ;
  • 윤경섭 (제주대학교 화학.코스메틱스학과)
  • Received : 2021.05.29
  • Accepted : 2021.06.21
  • Published : 2021.06.30
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Abstract

In order to delivery biotin to skin, ethosomes containing both biotin and ceramide were researched by using high pressure homogenizer. Biotin was utilized as a drug and ceramide NP was utilized as a supporter of bilayer. The biotin was entrapped in aqueous core, while ceramide NP was packed in the bilayer of the ethosomes. Looking at the physical properties of vesicles containing ceramide NP, the sized was 80~130 nm, the polydispersity index was 0.09~0.16, and the zeta potential was -40~-49 mV. In vesicles without ceramide NP, the size was 124.80±1.46 nm, and the zeta potential and polydispersity index were -45.48±1.27 mV and 0.088±0.018, respectively. Therefore, the ethosome with ceramide NP has improved physical properties of vesicles compared to the ethosome without ceramide NP. Skin absorption rates of ethosomes with ceramide NP were 6.13~14.98%, while skin absorption rate of ethosome without ceramide NP was 7.08% at 12 h. In conclusion, ethosomes containing ceramide NP not only improved the skin absorption efficiency, but had also a positive effect on the stability of vesicles.

바이오틴을 피부에 전달하기 위해 고압균질기를 사용하여 바이오틴과 세라마이드를 모두 함유하는 에토좀에 대하여 연구하였다. 바이오틴이 주된 성분으로 사용되었으며, 세라마이드 NP는 인지질 이중층의 지지체로 활용되었다. 바이오틴은 수용성 내부에 포획되었고, 세라마이드 NP는 에토좀의 이중층에 흡착되었다. 세라마이드 NP를 함유한 에토좀의 물성을 살펴보면 소포체의 크기는 80~130 nm, 다분산지수는 0.09~0.16, 제타 전위는 -40~-49 mV로 측정되었다. 세라마이드 NP가 없는 소포체의 크기는 124.80±1.46 nm, 다분산지수와 제타전위는 각각 0.088±0.018과 -45.48±1.27 mV이었다. 따라서 세라마이드 NP가 함유된 에토좀은 세라마이드 NP가 없는 에토좀에 비해 소포체의 물리적 특성이 개선됨을 알 수 있었다. 세라마이드 NP를 함유한 에토좀의 피부흡수율은 12시간 후 6.13~14.98%이었으며, 반면에 세라마이드 NP가 없는 에토좀의 피부흡수율은 7.08%이었다. 결론적으로 세라마이드 NP를 함유한 에토좀은 피부흡수 효율을 향상시킬뿐만 아니라 소포체의 안정성에도 긍정적인 영향을 미쳤다.

Keywords

Acknowledgement

This research was supported by the 2021 scientific promotion program funded by Jeju National University.

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