• Current Research on Agriculture and Life Sciences
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• 제5권
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• pp.158-167
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• 1987

The purpose of this study is to inquire and analyse the relation between traveltime (Tc) and watetshed physical characteristics surveyed such as river length (L), Lea, river main slope (s), base length of time area diagram, and storage constant (k). The results obtained in this study are as follows. The average widths of watersheds were with the range from 4.6 kilometers to 16.7 kilometers. The shape factors of main stream ranged from 0.08 to 0.37. The average slopes to main 8tream were within the range of 1.7-5.5 meter per kilometer. The relation between the base length and traveltime from S. C. S. method, Rational method, and RZIHA+KRAVEN method were derived $Tc=0.524{\times}1.35^c$ (r=0.98), $Tc=0.628{\times}1.339^c$, (r=0.98), $Tc=0.667{\times}1.342^c$ (r=0.97). The base length of the time-area diagram (c) for the IUH was derived as $c=0.9(\frac{L.L_{ca}}{\sqrt{s}})^{0.35}$ and correlation coefficient was 0.98 which defined a high significance. The storage constant K, derived in this study was $K=8.32+0.0213{\frac{L}{\sqrt{s}}}$ with correlation coefficient (0.96). The relation between storage Constant and conventional formula were figured out $Tc=0.0003{\times}3.323^k$ (r=0.97). $Tc=0.00045{\times}3.268^k$ (r=0.99) and $Tc=0.0004{\times}3.26^k$ (r=0.963). The base length (c) and storage constant (k) of time-Area Diagram were very important parts that determined traveltime for flood events. In the estimate of travel time for predicting flood volume, the formula of $Tc=0.524{\times}1.35^c$ that would be available to apply the Nak - Dong river watershed area and homogeneous watershed characteristics was found.

• 대한미생물학회지
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• 제13권1호
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• pp.43-48
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• 1978

항균제내성(抗菌劑耐性)인 Salmonella typhi를 공시(供試)하여 chloramphenicol(Cm), tetracycline(Tc), ampicillin(Ap), kanamycin(Km) 및 rifampicin(Rif)의 항균작용(抗菌作用)을 한천평판희석법(寒天平板稀釋法)(평판법(平板法))과 broth 희석법(稀釋法)(broth법(法))으로 비교(比較)하였다. 공시균(供試菌)의 최소발육저지농도(最少發育沮止濃度)(MIC)를 보면 Cm, Tc 및 Rif에 있어서는 평판법(平板法)과 broth법(法) 사이에 유의(留意)할만한 차이(差異)는 없었으나 Km에 있어서는 broth법(法)의 MIC가 평판법(平板法)보다 높은 것이 약(約) 반수(半數)있었고 Ap에 있어서도 소수(少數) 있었다. Cm과 Rif는 대부분(大部分)의 공시균(供試菌)에 대(對)하여 정균적(靜菌的)으로 작용(作用)하였으나 Tc, Ap 및 Km은 살균적(殺菌的)으로 작용(作用)하였고 그 작용(作用)은 균주(菌株)에 따라 차이(差異)가 있었다.

• 대한핵의학회지
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• 제26권2호
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• pp.346-354
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• 1992

서울대학교 의과대학에서 개발한 항 CEA항체에 대한 방사성옥소 및 $^{99m}Tc$의 표지법을 개발하고 이런 표지항체의 면역학적 특성을 분석하였다. 1) 8가지의 항 CEA의 단세포군 항체중 CEA-79항체와 CEA-92항체가 가장 좋은 면역학적 특성을 보였다. 표지항체의 면역반응성은 60% 정도이었고, 항원/항체 친화상수는 $1\sim2\times10^9l/m$ 이었다. 2) CEA-79항체는 chloramine-T법으로, CEA-92항체는 iodogen 법으로 방사성옥소를 표지 하는 것이 가장 좋은 표지효율 및 면역 반응성을 보였다. 3) CEA-79항체는 0.5 mCi/mg에서부터 100mCi/mg까지 비방사능을 변화시켰을 때 표지효율이나 면역 반응성에 유의한 차이는 발견할 수 없었다. 4) Glucarate를 이용한 pretargeting transchelation법으로 CEA-79항체를 $^{99m}Tc$로 표지하는 조건을 확립하였다. 표지효율은 $60\sim70%$ 이었다. 본 실험을 통하여 얻은 적절한 조건의 $^{131}I$과 $^{99m}Tc$표지방법의 확립은 앞으로 방사면역신티그라피와 방사면역치료법에 유용하게 사용될 수 있을 것으로 기대된다.

• 대한핵의학회지
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• 제33권1호
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• pp.84-93
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• 1999

목적: 주기율표상에서 7족에 위치하며, 유사한 화학적 특성을 지닌 $^{99m}Tc$와 $^{188}Re$의 항체에 표지하는 조건의 차이점과 앞서 표지된 항체의 핵종에 따른 거동의 차이를 비교 실험해 보고자 하였다. 대상 및 방법: mercaptoethanol을 사용하여 non-specific human IgG의 disulfide 결합을 환원시켜, -SH 기의 발생을 유도하였다. 앞서 처리된 IgG을 stannous ion 을 사용하여 $^{99m}Tc$과 $^{188}Re$을 환원하여 $^{99m}Tc$-IgG과 $^{188}Re$-IgG을 제작하였다. HPLC를 사용하여 $^{99m}Tc$-IgG은 1시간, 4시간. 6시간, 24시간마다 $^{188}Re$-IgG은 1시간, 4시간, 16시간, 24시간마다 시간경과에 따른 안정도 변화를 확인하였다. 결과: 한 분자당 약 2개의 -SH기를 유도 면역 활성이 최대한 유지되는 환원된 IgG를 얻을 수 있었다. 각각 90%와 95% 이상의 높은 표지반응수율로 $^{99m}Tc$-IgG과 $^{188}Re$-IgG을 얻었다. $^{99m}Tc$-IgG의 %peak area를 1시간, 4시간, 6시간, 24시간마다 측정한 값은 각각 91%, 83%, 78%, 7%이었다. $^{188}Re$-IgG의 경우 1시간, 4시간, 16시간, 24시간에서 94%, 80%, 47%, 42%이었다. $^{99m}Tc$-IgG을 정맥주사 후 4시간 후의 %ID/g는 장기별로 신장, 혈액, 위, 농양($9.42{\pm}0.68,\;1.43{\pm}0.24,\;0.86{\pm}0.18,\;0.72{\pm}0.10$)순이었고, 24시간에서는 신장, 농양, 위, 혈액($7.61{\pm}1.58,\;0.57{\pm}0.07,\;0.37{\pm}0.09,\;0.281{\pm}0.09$)의 순이었다. $^{188}Re$-IgG의 경우 4시간에서 신장, 혈액, 농양, 위($3.92{\pm}0.62,\;1.32{\pm}0.08,\;0.88{\pm}0.01,\;0.26{\pm}0.06$)의 순이였고, 24시간에서 신장, 농양, 혈액, 위($4{\pm}0.26,\;0.37{\pm}0.04,\;0.29{\pm}0.01,\;0.13{\pm}0.03$)의 순이었다. 결론: $^{99m}Tc$-IgG과 $^{188}Re$-IgG의 표지항체는 직접표지 법을 사용하여 효과적으로 제조되었다. 그러나, 직접표지법을 사용한 $^{99m}Tc$-IgG과 $^{188}Re$-IgG의 안정도는 시간경과에 따라 불안정함을 보여 안정성을 증가시키기 위한 항체표지 방법들에 대한 연구가 필요할 것으로 생각되었다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1991년도 추계학술대회 논문집 학회본부
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• pp.269-273
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• 1991

High Tc oxide superconductor with a Tc above 100 K has been successfully prepared by solid state reaction method in added-Pb BiSrCaCuO system. As compared with 123 compound, the formation reaction of the high Tc requires long time heat treatment. It is due to the transformation from the low Tc phase to high Tc phase. The sintering just below the melting point of the calcined powder mixture is effective on the formation of the high Tc phase in BiSrCaCuO system to be added with Pb. The growth of the high Tc superconducting phase has a thin plate shape, which is characterized by the c parameter of 37${\AA}$. The formation kinetics is also investigated in the samples with different Bi/Pb ratio and the 30% Pb addition is most preferable for the formation of the high Tc phase. The formation of the high Tc phases is delayed by the excessive addition of Pb. The lattice parameter(c) of the unit cell(both the low and high Tc phases) is increased with increase of Pb.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2006년도 제37회 하계학술대회 논문집 C
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• pp.1337-1338
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• 2006

BiSrCaCuO was prepared by the thermal pyrolysis method. The solid precursor produced by the dehydration of the gel at $120^{\circ}C$ for 12h is not in the amorphous state as expected but in a crystalline state. X-ray diffraction peaks of nearly the same angular position as the peaks of high Tc phase were observed in the precursor. After pyrolysis at $90^{\circ}C$ and calcination at $840^{\circ}C-920^{\circ}C$ for 4h, the high Tc phase was cleary observed. In this paper, the establishment of fabrication condition and additive effects of second elements were examined so as to improve the related properties to the practical use of BiSrCaCuO superconductor, and we reported the production of the BiSrCaCuO high Tc superconductor by the pyrolysis method.

• Journal of Microbiology and Biotechnology
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• 제16권2호
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• pp.205-211
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• 2006

A quantitative analytical method to detect new lines of genetically modified (GM) maize, NK603 and TC1507, has been developed by using a real-time polymerase chain reaction (PCR). To detect these GM lines, two specific primer pairs and probes were designed. A plasmid as a reference molecule was constructed from an endogenous DNA sequence of maize, a universal sequence of a cauliflower mosaic virus (CaMV) 35S promoter used in most GMOs, and each DNA sequence specific to the NK603 and TC1507 lines. For the validation of this method, the test samples of 0, 0.1, 0.5, 1.0, 3.0, 5.0, and 10.0% each of the NK603 and TC1507 GM maize were quantitated. At the 3.0% level, the biases (mean vs. true value) for the NK603 and TC1507 lines were 3.3% and 15.7%, respectively, and their relative standard deviations were 7.2% and 5.5%, respectively. These results indicate that the PCR method developed in this study can be used to quantitatively detect the NK603 and TC1507 lines of GM maize.

• 대한핵의학회지
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• 제23권1호
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• pp.49-54
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• 1989

Many previously described nuclear medicine procedures to assess glomerular filtration rate (GFR) required numerous blood samples obtained over a period of several hours to determine plasma concentrations of the injected radiopharmaceuticals. And other indirect methods of determining renal clearance have some problems due to individual variations in volume of distribution of the radionuclides used. Rescently reported Jackson's method have the great advantages that is a direct measurement method requiring less than 40 min of imaging time and single blood sampling. And it correctly accounts for individual variations in volume of distribution of the radiopharmaceuticals and can be done with routine renal scintrgraphy. We measured $^{99m}Tc-DTPA$ renal clearance with Jackson's method during the routine $^{99m}Tc-DTPA$ renal scintigraphy in 63 patients admitted to department of internal medicine in SNUH. In 23 cases among 63 patients creatinine clearence was accounted simultaneously. The range of $Cl_{DPDA}$ was from 19.9 ml/min to 170 ml/min and the correlation of $Cl_{DPDA}$ and creatinine clearance was discribed by Y=16.2570+0.7852 X($X=Cl_{DTPA}$ Y=creatinine clearance). And the correlation coefficient r was 0.88. We concluded that $^{99m}Tc-DTPA$ renal clearance measurement with Jackson's method was clinically useful to account GFR that can be done with routine $^{99m}Tc-DTPA$ renal scintigraphy simultaneously.

• Food Science and Biotechnology
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• 제17권3호
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• pp.569-572
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• 2008

A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 3 events of genetically modified (GM) maize. The event-specific primers were used to discriminate the following 3 events of GM maize (DAS-59122-7, TC6275, and MIR604) using multiplex PCR method. The zein gene was used as an endogenous maize reference gene in the multiplex PCR detection. The primer pair Zein-FIR producing a 99 bp amplicon was used to amplify the zein gene. The primer JI-Das-F1/R1 for DAS-59122-7, JI-TC6275-F3/R3 for TC6275, and JI-MIR F1/R1 for MIR604 yielded an amplicon of 130, 162, and 197 bp, respectively. The detection limit of multiplex PCR was 1% for DAS-59122-7, TC6275, and MIR604 for one reaction.

• KIEE International Transactions on Electrophysics and Applications
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• 제5C권5호
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• pp.217-220
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• 2005

We have fabricated a superconducting YBCO system according to the pyrolysis method and low pressure apparatus. In our experiment, the X-ray diffraction pattern of the non doped YBaCuO layer indicated that the superconductor contained only 90K phase crystal. The critical temperature and critical current density for a thick layer at $650^{\circ}C$ were Tc=90 K and $Jc=6{\times}10^{4}A/cm^2$ at 90K. In low pressure apparatus, the 90 K phase YBaCuO was grown at a lower temperature compared with the normal system. Tc and Jc at $650^{\circ}C$ were Tc = 90 K and $Jc=6{\times}10^{4}A/cm^2$ at 90K.