Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Quantitative Analysis of Two Genetically Modified Maize Lines by Real-Time PCR

  • Lee Seong-Hun (Division of Inspection, Experiment Research Institute of National Agricultural Products Quality Management Service) ;
  • Kang Sang-Ho (Gene Expression Team, National Institute of Agricultural Biotechnology) ;
  • Park Yong-Hwan (Gene Expression Team, National Institute of Agricultural Biotechnology) ;
  • Min Dong-Myung (Division of Inspection, Experiment Research Institute of National Agricultural Products Quality Management Service) ;
  • Kim Young-Mi (Gene Expression Team, National Institute of Agricultural Biotechnology)
  • Published : 2006.02.01
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Abstract

A quantitative analytical method to detect new lines of genetically modified (GM) maize, NK603 and TC1507, has been developed by using a real-time polymerase chain reaction (PCR). To detect these GM lines, two specific primer pairs and probes were designed. A plasmid as a reference molecule was constructed from an endogenous DNA sequence of maize, a universal sequence of a cauliflower mosaic virus (CaMV) 35S promoter used in most GMOs, and each DNA sequence specific to the NK603 and TC1507 lines. For the validation of this method, the test samples of 0, 0.1, 0.5, 1.0, 3.0, 5.0, and 10.0% each of the NK603 and TC1507 GM maize were quantitated. At the 3.0% level, the biases (mean vs. true value) for the NK603 and TC1507 lines were 3.3% and 15.7%, respectively, and their relative standard deviations were 7.2% and 5.5%, respectively. These results indicate that the PCR method developed in this study can be used to quantitatively detect the NK603 and TC1507 lines of GM maize.

Keywords

References

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