• KSBB Journal
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• 제5권3호
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• pp.207-214
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• 1990

Growth properties of carrot hairy roots transformed by Agrobacterium rhizogenes were compared in flask and bioreactor. Oxygen transfer coefficient KLa was measured during the cultivation in bioreactor. In flask cultures, initially sucrose 30g/l was nearly exhausted after 20days. pH was dropped from initially 5.8 to 4.79 after 4 days, but it is stable after that time. Finally, after 28 days, hairy roots were grown about twelve times. In view of the results studied optimum conditions, hairy roots were maintained high growth rates in sucrose 50g/l, pH 5.8, total nitrogen 60mM. Also in bioreactor cultures, fixed stainless sieve in bottom and aerated 0.31 vvm, the results of cultivation by the use of sucrose 50g/l had grown about twenty-eight times and pH variations were liked in flask. As a results, growth rate of 1.756g fresh weight/day/g inoculum in bioreactor were higher about three times than 0.57g fresh weight/day/g inoculum in flask culture. KLa values were showed a tendency to decrease from 0.209 min-1 to 0.068 min-1.

• 식물조직배양학회지
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• 제26권2호
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• pp.137-142
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• 1999

양액재배에 의한 조직배양 마늘의 종구 생산시 종구의 크기, 배양토 종류, 재식 깊이와 양액조성이 맹아 소요일수, 맹아율 및 생육에 미치는 영향에 대해 실험하였다. 양액종류, 종구의 크기에 관계없이 재식깊이를 1cm로 하였을 때 깊게 심은 (3cm)식재구에 비해 최초 맹아일수 및 맹아율이 크게 단축되었다. 반면 수확 후 종구의 직경, 무게 및 식물체 생체중은 재식 깊이 3cm로 하였을 때 다소 증가하였다. 종구의 크기는 맹아율, 맹아일수뿐만 아니라 식재 후의 생육에도 크게 영향을 미쳤는데 조직배양시 가능한 큰 종구를 생산하는 것이 토양이식 후 양호한 생육을 촉진할 수 있을 것으로 나타났다. 오리엔탈 양액 (N=0.17, P=0.45, K=1.29, Ca=2.44, and Mg=0.98 me/L)은 야마자끼 (N=0.27, P=0.16, K=1.50, Ca=1.36, and Mg=0.78 me/L) 양액보다 종구의 생장과 맹아율이 높았다. 식재용토로써 부엽, 바크, 모래를 혼합한 상토나 바로커를 이용하였을 때 수확 후의 종구의 직경, 무게 및 식물체 생체중이 현저히 증가하였다.

• 식물조직배양학회지
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• 제26권2호
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• pp.99-102
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• 1999

Glucose - induced arginine transport system was induced by a exogenous application of NH$_4$^+ ion. The uptake rate of arginine (Arg) depended on the external NH$_4$^+ ion concentration. The uptake rate was inhibited by the presence of NH$_4$^+ ion within 1 min, whereas it increased maximally after 30 min. Glucose and NH$_4$^+ ion induced the same arginine transport system. Km value of Arg transport systems was 2 $\mu$M, and V_(max) was 60 $\mu$mol^(-1) . h . g fresh weight^(-1) for NH$_4$^+ ion and 174 $\mu$mol^(-1) . h . g fresh weight^(-1) for glucose induced transport system. But, the transport system of Glu for glucose and NH$_4$^(-1) ion induced had different Km values. Km value of Glu was 285 $\mu$M for glucose - and 58 $\mu$M for NH$_4$^+ ion induced transport system. Thus, NH$_4$^+ ions play a important role as inducer for the glutamine transport system. NH$_4$^+ ion induced glutamine system was inhibited over 90% by cycloheximide. We concluded that a new carrier protein for glutamine was induced by NH$_4$^+ ion.

• Archives of Plastic Surgery
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• 제37권6호
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• pp.726-731
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• 2010

Purpose: Autologous fat grafting is a popular procedure for the correction of the soft tissue depression and deformity. But there are several issues required to be carefully considered in relation to this procedure, mainly about the unpredictability and the low survival rate of the grafted fat due to absorption and partial necrosis. Sphingosine-1-phosphate (S1P) is a lysophospholipid mediator that has been proposed to promote angiogenesis and to regulate the differentiation of adipose derived stromal cells (ASCs). In this study, we analyzed the viability of the grafted fat tissue mixed with S1P into each 12 nude mice (cann.cg-fox1nu/crlori) compared to the group of mice grafted fat tissue only. Methods: Human aspirated fat was grafted subcutaneously into the backs of 8-week-old nude mice with or without S1P. Eight weeks later, the grafted fat was harvested and the weight and volume were checked. The fat was stained with hematoxylin-eosin and 4',6-diamidino-2-phenylindole. Results: S1P group weighed significantly more than the control group (p=0.009), and the volume from the S1P group was considerably larger than that of the control group (p=0.004) either. In histological features, the surviving layer of the S1P group was thicker than the control group and microvasculature appeared to be prominent in the S1P group, especially in the outer layers. Conclusion: These findings suggest that S1P plays a vital role in the soft tissue augmentation, potentially providing a novel point of the control in adipose tissue for microfat graft.

• 생물환경조절학회지
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• 제15권1호
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• pp.35-46
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• 2006

Objective of this research was to determine the effect of application rate of a slow release fertilizer (SRF) in three root media, peatmoss+vermiculite (1:1, v/v; PV), peatmoss+composted rice hall (1:1, v/v; PR), and peatmoss+composted pine bark (1:1, v/v; PB), on growth and nutrient contents of potted chrysanthemum 'Lima Honey'. All media contained polyacrylic acid sodium salt at a rate of $4.5g L^{-1}$. The fresh and dry weights at 43 days after transplanting did not show statistical differences among treatments in each root media. Elevated application rate of SRF increased fresh and dry weights at 80 days after transplanting in PV and PB media, but not in PR medium. Elevated application rates of SRF resulted in the increase of tissue phosphorus content and decrease of tissue Ca, Na, and Zn contents at both 43 and 80 days after transplanting. Elevated application rates of SRF resulted in the decrease of pH and increase of EC and concentrations of ${NO_3}^-$ and ${P_2O_5}^{3-}$, K, Ca, and Mg in the soil solution of PV and PR media. The trends of those in PR media were also similar except ${NO_3}^-$. The differences among treatments in EC at 80 days after transplanting were less significant as compared to those at 43 days after transplanting in three media.

• 운동영양학회지
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• 제16권2호
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• pp.85-92
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• 2012

This study was conducted to examine the possibility of Salicornia herbacea L. powder as a functional food beneficially affecting carbohydrate metabolism and antioxidant capacity. Twenty-four, 6-week old, maleSprague-Dawleyrats were classified into three groups: normal diet control (CON), high-fat diet control (HFC) and high fat diet + Salicornia herbacea L. (SHF). Related feed was provided to each group for 4 weeks. Weight gain rate increased most in the HFC rats, and the concentration of glucose was significantly high in both the HFC and SHF groups, compared to the CON group. The SHF group showed a significantly high expression rate of Glut 4 (21.36%), compared to the CON and HFC groups. The glycogen content in muscle was significantly high in both the HFC and SHF groups, compared to the CON group. There were significant differences in the malondialdehydecontent in muscles between the groups, with the content in the CON and HFC groups being significantly higher than the SHF group. All the groups showed a similar tendency to each other in the liver tissue as well. Concerning the expression of Cu,Zn-super oxide dismutase andglutathione peroxidaseproteins, the SHF group was significantly higher than the CON and HFC groups. Overall, the experiment result above implies a possibility that an intake of Salicornia herbacea L. powder can regulate weight by decreasing the weight gain rate, further suggesting its effectiveness as a functional food before exercise by increasing the energy storage capacity and antioxidant capacity.

• The Korean Journal of Physiology
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• 제6권2호
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• pp.9-17
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• 1972

This experiment was carried out to investigate systematically how the aerobic metabolic capacity of renal tissue reduced by the effects of a period of induced ischemia. Aerobic metabolic studies were performed on homogenates of cortex and medulla of rabbits. Ischemia was induced by occluding the renal vein or renal artery of the left kidney for an hour. The right kidney used as a paired control. Aerobic metabolism was asesssed by measuring the oxygen consumption using the Warburg's manometric apparatus. The results are summarized as follows: 1. One hour of occlusive ischemia does not increase in the kidney weight in the renal arterial occlusion but increase in the renal venous occlusion. 2. Occlusion of either the renal vein or renal artery for an hour did not reduce to any significant degree the level of endogenous substrate in cortical homogenates as measured the rates of $0_2$ consumption. 3. A significant reduction in the rate of $C_2$ consumption was noted in the medullary homogenates of renal venous occluded kidneys while renal arterial occlusion had less of an effect. 4. The capaciy of homogenates for aerobic metabolism is not reduced by acute ischemia, because of the higher rate of oxygen consumption induced by exogenous glucose in renal vein occlusion. 5. The oxygen consumption of medullary homogenate more decreased to acute ischemia than cortical homogenates. The results of this investigation suggest that one hour circulatory stasis does not reduce major potential capacity of renal cortical tissue at the subcellular level to produce energy. In contrast, the aerobic metabolism of medullary tissue is reduced by renal ischemia. Further, both cortex and medulla appear to be more sensitive to ischemia induced by renal venous occlusion than by renal arterial occlusion.

• 대한한의학방제학회지
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• 제17권2호
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• pp.133-142
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• 2009

Objective : Recently, obesity has increased at an alarming rate and is now a worldwide health problem. Natural dietary supplement, $CharmSlim^{(R)}$(C), is mixed pills, which consists of beans, black sesames, kelps, onions, adlay seeds, anchovies, pyogo mushrooms, green tea. Our study was performed to determine the weight-loss effects of natural dietary supplement, based on natural ingredients, in mice fed high-fat diet with an abundance of carbohydrates. Methods : Four groups of male ICR mice were fed different diets during 6 weeks: normal diet(NOR), high-fat (15%, w/w) diet(HF), high-fat supplemented with natural dietary supplement powdered 5%(C5) and 10%(C10) groups. We measured the changes of food intake, body weight and adipose tissues weights. Also we examined levels of lipid profiles in serum. Results : Body weight, liver weight and food efficiency ratio were significantly higher in the HF group than in NOR group. After 6 weeks of treatment, body weight, liver weight and adipose tissues weights (epididymal, perinephric, visceral and BAT) were significantly decreased in the C10 group when compared to the control HF group. The C10 group had markedly lower serum levels of total cholesterol and triglyceride when compared with HF group. Conclusion : These results demonstrate that this dietary supplement can suppress the increased lipid profiles, body weight and fat gains, thus could be considered as an effective adjunct in the treatment of obesity.

• International Journal of Contents
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• 제7권2호
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• pp.26-31
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• 2011

People with obesity or over-weight need nutritional intervention to reduce their weight, because weight loss reduces the incidence rate of chronic diseases such as hypertension, type II diabetes, cardiovascular diseases and cancer in obese people. This study was to develop a system for individualized weight control program available both for wired and wireless internet users. This system is especially useful to users carrying wireless internet mobile device. If they input their physical information (height, weight and waist circumference) and mineral levels measured by hair tissue mineral analysis, the system provides evaluation of their health status and metabolic related functions such as endocrine and carbohydrate tolerance. Based on these evaluations, food menus are then offered to them to manage their health status and to improve their metabolic related physiological functions in a personalized way. The system also provides more information for recommended foods, such as nutritional information, food ingredients, recipes, and videos related to cooking. Bibimbap was selected as an example dish for customized contents for mobile web. Bibimbap is one of the most well-known Korean traditional dishes prepared with various kinds of ingredients including several different kinds of vegetables, meat, and egg so that it is a low calorie dish as well as a well-balanced diet. Therefore, this system developed in this study allows the mobile users to access web site through wired wireless internet everywhere and provides a customized content to the users to manage their weight and finally to achieve a desirable weight.

• Journal of Nutrition and Health
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• 제6권1호
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• pp.15-29
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• 1973

Albio rats right after weaning, weighing $50{\sim}55g$, were divided into the control, high-carbohydrate-, high-lipid-, and high-protein-fed groups. and were fed for 12 weeks with the respective diets to observe the increase in body weights as well as changes in the chemical constituentes and enzyme activities in the liver tissue, with the following results. (1) There was little difference in the rate of increase in the body weights among the groups, showing normal growth, except the high-protein-fed group which showed decrease in rate of body weight increase from the 7th week after feeding. (2) The liver weight was either increased after 12 weeks of feeding with the high-carbohydrate and high-Lipid diets, or showed no difference with the high-protein diet, as compared to the control weight. (3) The liver cytosol protein content was increased when fed with the high-protein diet, but decreased when fed with the high-carbohydrate and high lipid diets, as compared to the control content. (4) The triglyceride and cholesterol contents in the liver were decreased in the high-protein-fed group, but increased markedly in the high-carbohydrate- and high-lipid-fed groups as compared to the control values. (5) The hepatic glucokinase, G6PD, LDH, and fatty acid synthetase activities were increased in the high-carbohydrate and high-lipid-fed groups, and GOT and CPT activities were increased in the high-protein-fed group. From the above results. it was known that the high-carbohydrate and high-lipid diets stimulated the hepatic lipid metabolism, giving rise to lipogenesis, but the high- protein diet could prevent the lipogeuesis leading to the body weight increase.