Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
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The Effect of Sphingosine-1-Phosphate in Survival of Grafted Fat Tissues

Sphingosine-1-Phosphate (S1P)가 이식지방의 생존에 미치는 영향

  • Yoon, Dong-Ju (Yoon Dong Ju Plastic Surgery Clinic) ;
  • Choi, Soo-Jong (Department of Plastic and Reconstructive Surgery, School of Medicine, Pusan National University) ;
  • Choi, Chi-Won (Department of Plastic and Reconstructive Surgery, School of Medicine, Pusan National University) ;
  • Nam, Su-Bong (Department of Plastic and Reconstructive Surgery, School of Medicine, Pusan National University) ;
  • Bae, Yong-Chan (Department of Plastic and Reconstructive Surgery, School of Medicine, Pusan National University)
  • 윤동주 (윤동주 성형외과) ;
  • 최수종 (부산대학교 의학전문대학원 성형외과학교실) ;
  • 최치원 (부산대학교 의학전문대학원 성형외과학교실) ;
  • 남수봉 (부산대학교 의학전문대학원 성형외과학교실) ;
  • 배용찬 (부산대학교 의학전문대학원 성형외과학교실)
  • Received : 2009.12.18
  • Accepted : 2010.04.22
  • Published : 2010.11.10
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Abstract

Purpose: Autologous fat grafting is a popular procedure for the correction of the soft tissue depression and deformity. But there are several issues required to be carefully considered in relation to this procedure, mainly about the unpredictability and the low survival rate of the grafted fat due to absorption and partial necrosis. Sphingosine-1-phosphate (S1P) is a lysophospholipid mediator that has been proposed to promote angiogenesis and to regulate the differentiation of adipose derived stromal cells (ASCs). In this study, we analyzed the viability of the grafted fat tissue mixed with S1P into each 12 nude mice (cann.cg-fox1nu/crlori) compared to the group of mice grafted fat tissue only. Methods: Human aspirated fat was grafted subcutaneously into the backs of 8-week-old nude mice with or without S1P. Eight weeks later, the grafted fat was harvested and the weight and volume were checked. The fat was stained with hematoxylin-eosin and 4',6-diamidino-2-phenylindole. Results: S1P group weighed significantly more than the control group (p=0.009), and the volume from the S1P group was considerably larger than that of the control group (p=0.004) either. In histological features, the surviving layer of the S1P group was thicker than the control group and microvasculature appeared to be prominent in the S1P group, especially in the outer layers. Conclusion: These findings suggest that S1P plays a vital role in the soft tissue augmentation, potentially providing a novel point of the control in adipose tissue for microfat graft.

Keywords

References

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