• 생약학회지
• /
• 제7권1호
• /
• pp.55-57
• /
• 1976

The callus formation of Glycyrrhiza glabra L. var. $glandulifera\;R_{EG}.\;et\;H_{ERD}$. in tissue culture was promoted on Murashige and Skoog's basal solution supplemented with 40g/l of sucrose, 1mg/l of kinetin and 5mg/l of 2, 4-D. The fresh and dry weights of callus and glycyrrhizin contents in callus of the Glycyrrhiza glabra L. var. $glandulifera\;R_{EG}.\;et\;H_{ERD}$. were determined monthly up to 12 months and obtained the results as follows: 1.The fresh weight of formed callus was increased rapidly from 2 to 4 months but growing rate of callus was slow from 4 to 6 months. This indicates that the cell division of callus was most active during the first $2{\sim}3$ months. 2. Glycyrrhizin contents in callus were also increased but the contents were not related to the increased weight of callus.

• 생물환경조절학회지
• /
• 제15권1호
• /
• pp.8-20
• /
• 2006

This research was conducted to determine the plant growth and nutrient contents of potted chrysanthemum 'Lima Honey' as influenced by application rate of dolomite in three root media, peatmoss+vermiculite (1:1, v/v), peatmoss+composted rice hall (1:1, v/v), and peatmoss+composted pine bark (1:1, v/v). All root media contained polyacrylic acid sodium salt (PASS) at a rate of $4.5g L^{-1}$. In crop growth at 40 and 80 days after transplanting, elevated application rates of dolomite up to $6.0g L^{-1}$ in three root media increased fresh and dry weights per plant. But the treatment of $9.0g L^{-1}$ had less fresh and dry weights than those of $6.0g L^{-1}$. Tissue phosphorus content decreased and those of Ca and Mg increased by the elevated application rates of dolomite. Elevated application rates of dolomite in three root media resulted in the increase of pH, EC, and the concentrations of K, Ca, and Mg at 43 days after transplanting. Those at 80 days after transplanting were also increased, but the differences among treatments were less significant as compared to those at 43 days after transplanting. From the results of dry weight in potted chrysanthemum 'Lima Honey', the proper application rate of dolomite to three root media containing PASS at a rate of $4.5g L^{-1}$ were $6.0g L^{-1}$.

• 식물조직배양학회지
• /
• 제26권4호
• /
• pp.229-233
• /
• 1999

본 연구에서는 낙엽송 배발생조직의 장기저장을 위한 가능성에 대하여 조사하였다. 0.4M혹은 20% PEG로 24시간 전 처리하여 -0.33$^{\circ}C$/min의 동결온도 하강율로 초저온시켰을 때 높은 조직생중량을 보인 반면 -0.5 혹은 -1.$0^{\circ}C$/min의 경우 초저온 후 조직의 생중량이 저조한 것으로 나타났다. 1, 7, 및 28일간 초저온보존된 조직을 회수하여 재생장시킨 다음 PCR을 이용하여 변이분석을 한 결과 초저온보존 기간에 관계없이 DNA변이는 전혀 나타나지 않았다. 초저온보존된 배발생 조직으로부터 체세포배 유도 및 식물체 재분화를 유도할 수 `있었다.

• 대한방사선기술학회지:방사선기술과학
• /
• 제11권2호
• /
• pp.3-15
• /
• 1988

When X-rays were projected into a patient, there occured the phenomena such as penetration, absorption and scattering etc. The penetrating rays were recorded on films as X-ray image used for diagnosis but scattered rays caused the radiation hazard both to the patient, specialist and technicians. The soft tissue includes many organs which are sensitive to the radiation and in may occupy $40{\sim}50%$ of body weight. Therefore X-rays should be carefully projected to the patient and it is strongly recommended to analyse the distribution of X-rays, when ever the patient is exposed to X-rays. In this study, the distribution of X-ray according to the thickness, the radiation field and the tube voltages (kVp) in soft tissue, the following results were obtained: 1. Total transmitted rays which kept the step with X-ray tube voltage (kVp) increased in proportion to the increasing of X-ray tube voltage. 2. The scattered ray rate in the total transmitted ray was not significantly found with X-ray tube voltage. 3. The affecting factors of the scattered ray rate in total transmitted ray were shown through the radiation field and the thickness. 4. The dose of scattered ray by the angle was observed more in direction of primary ray ($0^{\circ}$) and back scattering ($160^{\circ}$) than in direction of $90^{\circ}$. 5. The more the distance from phantom to the patient should be less distribution of scattered ray.

• Advances in Traditional Medicine
• /
• 제7권3호
• /
• pp.254-260
• /
• 2007

Alstonia scholaris (Family: Apocynaceae) has been indicated for the treatment of various diseases, one among which is wound healing. The purpose of this study is to investigate the wound healing effect and antioxidant role of Alstonia scholaris leaves in animal models. The ethanol and aqueous extracts of Alstonia scholaris (EEAS, AEAS respectively) were tested against excision, incision and dead space wound models to evaluate the wound healing activity. In excision wound model, treatment was continued till the complete healing of wound, in incision and dead space wound models, the treatment was continued for 10 days. For topical application, 5% w/w ointment of EEAS and AEAS were prepared in 2% sodium alginate. For oral administration, EEAS was suspended in distilled water using Tween 80 and AEAS was dissolved in distilled water. The wound healing was assessed by the rate of wound contraction, period of epithelialisation, skin breaking strength, granulation strength, dry granulation tissue weight, hydroxyproline, collagen and histopathology of granulation tissue. Malondialdehyde level was also estimated to evaluate the extent of lipid peroxidation. AEAS and EEAS significantly promoted wound healing activity in all the wound models studied. Increase in the rate of wound contraction, skin breaking strength, granulation strength, dry granulation tissue weight, hydroxyproline and collagen, decrease in the period for epithelialisation and increased collagenation in histopathological section were observed with EEAS and AEAS treated groups. EEAS and AEAS also significantly decreased the levels of lipid peroxidation. The present study is suggestive that EEAS and AEAS promote wound healing activity.

• 생물환경조절학회지
• /
• 제15권1호
• /
• pp.21-34
• /
• 2006

This research was conducted to determine the plant growth and nutrient contents of potted chrysanthemum 'Lima Honey' as influenced by application rate of fused superphosphate (FSSP) in three root media, peatmoss+vermiculite (1:1, v/v; PV), peatmoss+composted rice hall (1:1, v/v; PR), and peatmoss+composted pine bark (1:1, v/v; PB). All root media contained polyacrylic acid sodium salt at a rate of $4.5g L^{-1}$. The treatment of $1.4g L^{-1}$ in PV and those of $0.7g L^{-1}$ in PR and PB had the greatest fresh and dry weights in each root medium at both 43 and 80 days after transplanting. Elevated application rates of FSPP increased tissue contents of N, P, and K at both 43 and 80 days after transplanting in PV medium. However, the differences in tissue contents of N, P and K in PR medium were less significant among treatments of FSPP. The pre-planting FSPP also less affected the tissue contents of nutrients at 80 days after transplanting as compared to those at 43 days after transplanting. Elevated application rates of FSPP in PV medium increased EC and the concentrations of $NO_3,\;P_2O_5$, K, Ca, and Mg in soil solution of root media at 43 days after transplanting. The EC in PV medium at 80 days after transplanting was higher than that at 43 days after transplanting. The EC in all root media at 80 days after transplanting was not different among treatments of FSPP.

• 대한한방소아과학회지
• /
• 제21권2호
• /
• pp.51-67
• /
• 2007

Objectives The study was designed to find out the relationships between short stature and low weight among children and teenagers. Methods This research was performed in 21 children and teenagers from August 2006 to June 2007. We measured height, weight, body mass index, obesity rare, bone age, and HTMA. Moreover, we analyzed correlation with height, weight, mineral concentrations and ratios obtained from HTMA. Results There were significant correlations between HTMA and short stature and low weight. 1. Most subjects(76%) had slow metabolic types. 2. Ca, Mg, Zn, Ca/P, and Cr had significant differences between high bone age group and high chronological age group. 3. Fe/Cu had significant differences between a group that recent height is more smaller than inherited height and a group that recent height is more taller than inherited height. 4. Ca, Mg, Ca/P had significant differences between a low weight group and a normal weight group classified by obesity rate. Conclusions Children and teenagers with short stature and low weight have specific mineral characters.

• Archives of Plastic Surgery
• /
• 제41권5호
• /
• pp.535-541
• /
• 2014

Background Patients having unilateral breast reconstruction often require a second stage procedure on the contralateral breast to improve symmetry. In order to provide immediate symmetry and minimize the frequency and extent of secondary procedures, we began performing simultaneous contralateral balancing operations at the time of initial reconstruction. This study examines the indications, safety, and efficacy of this approach. Methods One-hundred and two consecutive breast reconstructions with simultaneous contralateral balancing procedures were identified. Data included patient age, body mass index (BMI), type of reconstruction and balancing procedure, specimen weight, transfusion requirement, complications and additional surgery under anesthesia. Unpaired t-tests were used to compare BMI, specimen weight and need for non-autologous transfusion. Results Average patient age was 48 years. The majority had autologous tissue-only reconstructions (94%) and the rest prosthesis-based reconstructions (6%). Balancing procedures included reduction mammoplasty (50%), mastopexy (49%), and augmentation mammoplasty (1%). Average BMI was 27 and average reduction specimen was 340 grams. Non-autologous blood transfusion rate was 9%. There was no relationship between BMI or reduction specimen weight and need for transfusion. We performed secondary surgery in 24% of the autologous group and 100% of the prosthesis group. Revision rate for symmetry was 13% in the autologous group and 17% in the prosthesis group. Conclusions Performing balancing at the time of breast reconstruction is safe and most effective in autologous reconstructions, where 87% did not require a second operation for symmetry.

• The Korean Journal of Physiology
• /
• 제1권1호
• /
• pp.33-41
• /
• 1967

The metabolic patterns of C-1 and C-6-carbon atoms of glucose were observed in the tissue homogenates of the Ehrlich ascites tumor tissue which was incubated for 3 hours in the Dubnuff metabolic shaking incubator. $C^{14}-1-and\;C^{14}-6-glucose$ were used as tracers. The glucose media in which tissue homogenate was incubated was kept at a concentration of 200mg% glucose of carrier and appropriate amount of $C^{14}-1-or\;C^{14}-6-tracer$. At the end of 3 hour incubation, respiratory $CO_2$ samples trapped by alkaline which is placed in the tenter well of incubation flask were analyzed for the total $CO_2$ production rates and their radioactivities. The tissue homogenate samples after incubation were analyzed for their concentrations of glucose, lactate, pyruvate and glycogen and calculations were made on the glucose consumption rate, pyruvate and lactate accumulation rates. The following results were obtained. Data obtained in each group are as follows: 1. In the tissue homogenate, which was incubated with $C^{14}-1-glucose as a substrate, total $CO_2$ production rate averaged $19.0{\pm}5.0{\mu}M/hr/gm$ and the mean specific activity of respiratory $CO_2$ was $840{\pm}296\;cpm/mgC.$ Relative specific activity (RSA) which means the fraction of $CO_2$ derived from medium $C^{14}-1-glucose$ to total $CO_2$ production rate was calculated by ratio of SA of respiratory $CO_2$ and medium $C^{14}-1-glucose.$ RSA was $14.3{\pm}5.0%,$ Accordingly actual $CO_2$ production rate from medium $C^{14}-1-glucose$ showed a mean value of $2.79{\pm}1.35\;{\mu}m$ of which amount was equivalent to the mean value of total glucose consumption rate $(RGDco_2)$, namely, $5.1{\pm}1.3%.$ Lactate and pyruvate appearance rates averaged $7.13{\pm}1.26\;and\;0.21{\pm}0.02{\mu}M/hr/gm,$ respectively. Assuming that these 3 carbon compounds appeared in the medium were derived from glucose, calculations were made that relative glucose disappearance rate into lactate $(RGD_L)$ was $38.0{\pm}5.4%\;and\;RGD_P$ was $1.23{\pm}0.03%.$ Therefore, about 43.3% of the total glucose consumed were accounted for by conversion into the respiratory $CO_2$, lactate and pyruvate. 2. In the second group, which was incubated with $C^{14}-1-glucose$ as a substrate, glucose consumption rate, lactate and pyruvate appearance rates showed almost the same order as the values of the $C^{14}-1-glucose$ substrate group. However, RSA was remarkably decreased showing a mean value of $1.02{\pm}0.13%.$ This fact means that the C-6 carbon of glucose take the minor part in the oxidative metabolism of glucose. The glycogen level in both substrate tissue homogenate showed less than 0.3% of tissue weight. These low value suggested that there was an inhibition of carbohydrate synthesis in the Ehrlich ascites tumor tissue. 3. The catabolic pathway of glucose in the tumor tissue were analyzed on the basis of Bloom's principle from the values of RSA. It was found that in the tumor tissue more than 90% of $CO_2$ derived from glucose were oxidized via the alternate pathway other than principal EMP-TCA cycle such as hexose monophosphate pathway (HMP). From the data described above, it was assumed that in the Ehrlich tumor tissue anaerobic glycolysis proceeds normally although, the oxidation of products of anaerobic glycolysis via the TCA cycle is inhibited resulting in the accumulation of lactate and almost all of oxidative energy from glucose is released by oxidative pathway such as HMP.

• 한국초지조사료학회지
• /
• 제8권2호
• /
• pp.104-109
• /
• 1988

A field experiment was conducted in order to investigate the seasonal changes of leaf grwoth and related characteristics in three cultivars of orchardgrass; Potomac, Kay and Sumas. The results were summarized as follows: 1. Leaf elongation was increased in a nearly linear phase during first and third cutting stages. It was increased slowly in early 10 days to 15 days after cutting and increased rapidly there-after during the rest cutting stages. In cultivars, Potomac was showed higher leaf elongation than other cultivars during all cutting stages. There was no difference of leaf width within cutting stages, but the leaf width of fall regrwoth was narrow. Sumas had relatively short and wide leaves. 2. Leaf dry weight and leaf area in first cutting stage were larger than others. Leaf area was increased rapidly form 15 days after cutting and leaf weight was increased rapidly from 20 days over all cutting stages. The increase in leaf area and dry weight were slow down after 30 days. 3. Number of epidermal cells was increased rapidly after cutting and the rate of increase was slow down after 30 days. In a cross section of leaf tissue, the part of mesophyll was occupied with about 60% of total area and larger area than other tissue, the part of mesophyll was occupied with about 60% of total area and larger area than other tissues. Leaf tissue had a large vacancy at early growth period after harvest and was filled gradually with mesophyll. This result was related to the increase of leaf dry matter.