Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지

Cryopreservation of Embryogenic Tissue and Plant Regeneration in Larix leptolepis

낙엽송 (Larix leptolepis) 배발생조직의 초저온보존 및 식물체 재분화

  • Kim, Yong-Wook (Department of Tree breeding, Forestry Research Institute) ;
  • Kim, Joon-Chul (Department of Biology, Kangwon National University) ;
  • Youn, Yang (Department of Tree breeding, Forestry Research Institute) ;
  • Noh, Eu-Rae (Department of Tree breeding, Forestry Research Institute) ;
  • Son, Sung-Ho (Department of Tree breeding, Forestry Research Institute)
  • 김용욱 (임업연구원 임목육종부) ;
  • 김준철 (강원대학교 생물학과) ;
  • 윤양 (임업연구원 임목육종부) ;
  • 노의래 (임업연구원 임목육종부) ;
  • 손성호 (임업연구원 임목육종부)
  • Published : 1999.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

The possibility for long-term preservation of Larix leptolepis embryogenic tissue was tested in this study. Higher relative increase of the tissue fresh weight was observed when embryogenic tissue was pretreated for 24 hrs in a medium containing 0.4 M sorbitol or 20% polyethyleneglycol with cooling rate of -0.33$^{\circ}C$/min. The fast cooling rate of -0.5$^{\circ}C$ and -1.$0^{\circ}C$/min appeared to be less effective in regrowth of tissues from cryopreservation. No DNA variants have been observed by PCR analysis among the embryogenic tissues recovered after 1-, 7-, and 28-day-cryopreservation. The post-thaw embryogenic tissue gave rise to mature somatic embryos which developed into plants.

본 연구에서는 낙엽송 배발생조직의 장기저장을 위한 가능성에 대하여 조사하였다. 0.4M혹은 20% PEG로 24시간 전 처리하여 -0.33$^{\circ}C$/min의 동결온도 하강율로 초저온시켰을 때 높은 조직생중량을 보인 반면 -0.5 혹은 -1.$0^{\circ}C$/min의 경우 초저온 후 조직의 생중량이 저조한 것으로 나타났다. 1, 7, 및 28일간 초저온보존된 조직을 회수하여 재생장시킨 다음 PCR을 이용하여 변이분석을 한 결과 초저온보존 기간에 관계없이 DNA변이는 전혀 나타나지 않았다. 초저온보존된 배발생 조직으로부터 체세포배 유도 및 식물체 재분화를 유도할 수 `있었다.

Keywords

References

  1. CR Acad Sci Ser v.310 Efficient regeneration of plantlets from embryogenic callus of Picea abies (L) Karst after freezing in liquid nitrogen Bercetche J;Galerne M;Dereuddre J
  2. Rep PI-X-120 Tree germplasm preservation using biotechnology. In: Charest PS, Duchesne LC,(eds), Recent Progress in Forest Biotechnology in Canada Charest PJ;Klimaszewska K
  3. Martinus Nijhoff, Dordrecht v.2 Cryopresevation of woody species. In: Bonga JM, Durzan DJ, (eds), Cell and Tissue. Culture in Forestry Chen THH;Kartha KK
  4. Plant Cell Rep v.53 Crypreservation of interior spruce(Picea glauca engelmanii complex) embryogenic cultures Cyr DR;Lazzaroff WR;Grimes SMA;Quan G;Bethune TD;Dunstan DI;Robers DR
  5. Scand J For Res v.8 Cryopreservation of an embryogenic sustension culture of Picea sitchensis and subsequent plant regeneration Find JI;Floto F;Krogstrup P;Moller JD;Norgaard JV;Kristensen MH
  6. Bio/Tech v.5 Biotechonlogly of somatic polyembryogenesis and plantlet regeneration in loblolly pine Gupta PK;Durzan DJ
  7. Can J For Res v.17 Somatic polyembryogenesis in embryogenic cell masses of Picea abies)Norway spruce) and Pinus taeda (loblolly pine) after thawing form liquid nitrogen Gupta PK;Durzan DJ;Finkle BJ
  8. J Plant Physiol v.132 Induction of smoatic embryos and plantlets from cryopreserved cell cultures of white spruce (Picea glauca) Kartha KK;Fowke LC;Leung NL;Caswell KL;Hakman I
  9. J Exp Bot v.43 Crypreservation and plant regeneration from embryogenic cultures of larch (Larix × eurolepis) and black spruce (Picea mariana) Klimaszewska K;Ward C;Cheliak WM
  10. Plant Cell Rep v.11 Recovery of plants from cryopreserved embryogenic cell suspensions of Pinus caribaea Laine EP;Bade P;David A
  11. Plant Cell Rep v.4 Influence of a lobolly pine (Pinus taeda L.) culture medim and its components on growth and comatic embryogenesis of the sild carrot (Darcus carota L.) Litvay JD;Verma DC;Johnson MA
  12. Can J For Res v.23 Variations in cryotolerance of embryogenic Picea abies cell lines and the association to geneic morphological, and physiological factors Norgaard JV;Duran V
  13. Theor Appl Genet v.89 Somatic embryogenesis in white spruce (Picea glauca): genetic control in somatic embryos exposed to storage, maturation treatments, germination, and cryopreservation Park YS;Pond SE;Bonga JM