• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제41권1호
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• pp.11-18
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• 2015

Objectives: The goal of this study was to determine the correlation of clinicopathological factors and the up-regulation of vascular endothelial growth factor (VEGF) expression in oral squamous cell carcinoma. Materials and Methods: Immunohistochemical staining of VEGF and quantitative real-time polymerase chain reaction (RT-PCR) of VEGF mRNA were performed in 20 specimens from 20 patients with oral squamous cell carcinoma and another 20 specimens from 20 patients with carcinoma in situ as a controlled group. Results: The results were as follows: 1) In immunohistochemical study of poorly differentiated and invasive oral squamous cell carcinoma, high-level staining of VEGF was observed. Significant correlation was observed between immunohistochemical VEGF expression and histologic differentiation, tumor size of specimens (Pearson correlation analysis, significance r>0.6, P<0.05). 2) In VEGF quantitative RT-PCR analysis, progressive cancer showed more VEGF expression than carcinoma in situ. Paired-samples analysis determined the difference of VEGF mRNA expression level between cancer tissue and carcinoma in situ tissue, between T1 and T2-4 (Student's t-test, P<0.05). Conclusion: These findings suggest that up-regulation of VEGF may play a role in the angiogenesis and progression of oral squamous cell carcinoma.

• 현장농수산연구지
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• 제19권1호
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• pp.75-82
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• 2017

식용백합 Lilium brownii의 국내 생육특성과 인편조직배양에 의한 자구 발생수, 자구중, 자구중은 다음과 같다. 노지조건에서는 5월 25일 개화하였고 무가온하우스는 5월 18일이었으며 초장은 노지 50±3cm, 하우스 54±3cm 였다. 인편착생 위치별 자구 발생수, 자구중, 자구중은 외인편>중인편>내인편 순으로 좋았다. 인편 크기별로는 대인편에서 3.5개로 가장 많았고 인편이 클수록 자구중, 자구중이 좋았다. 대량생산을 위한 BA 농도별 처리는 BA. 1.5mg/l에서 3.8개로 가장 많았고 자구중 및 자구경도 좋았다.

• Carbon letters
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• 제13권3호
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• pp.139-150
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• 2012

Poly-L-lactic acid (PLLA), PLLA/hydroxyapatite (HA), PLLA/multiwalled carbon nanotubes (MWNTs)/HA, PLLA/trifluoroethanol (TFE), PLLA/gelatin, and carbon nanofibers (CNFs)/${\beta}$-tricalcium phosphate (${\beta}$-TCP) composite membranes (scaffolds) were fabricated by electrospinning and their morphologies, and mechanical properties were characterized for use in bone tissue regeneration/guided tissue regeneration. MWNTs and HA nanoparticles were well distributed in the membranes and the degradation characteristics were improved. PLLA/MWNTs/HA membranes enhanced the adhesion and proliferation of periodontal ligament cells (PDLCs) by 30% and inhibited the adhesion of gingival epithelial cells by 30%. Osteoblast-like MG-63 cells on the randomly fiber oriented PLLA/TEF membrane showed irregular forms, while the cells exhibited shuttle-like shapes on the parallel fiber oriented membrane. Classical supersaturated simulated body fluids were modified by $CO_2$ bubbling and applied to promote the biomineralization of the PLLA/gelatin membrane; this resulted in predictions of bone bonding bioactivity of the substrates. The ${\beta}$-TCP membranes exhibit good biocompatibility, have an effect on PDLC growth comparable to that of pure CNF membrane, and can be applied as scaffolds for bone tissue regeneration.

• Journal of Periodontal and Implant Science
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• 제29권2호
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• pp.355-373
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• 1999

Many researches have been reported that collagen as cellular stroma, matrix of grafting materials, mediator of agents for the purpose of promoting healing process invivo, but the responses in vivo were seen various. The goal of this experiment is to assess the effect of collagen on bony healing, through histological evaluation of implanted collagen on the calvarial defect in rats. 2-month-old Sprague-Dawley, 24 rats were used and 12 rats assigned to each group of control and test. Defect of 5mm in diameter was made on the calvarial bone with trephine bur. Following thorough saline rinse, defect of control group was left in empty and that of experimental group was filled with fibrillar collagen($COLLATAPE^{(R)}$, COLLA-TEC. INC. U.S.A.) soaked in saline. 3 rats in each group were sacrificed at 3, 7, 14, 21 days after operation respectively, and the tissue blocks were prepared for light microscope with H-E for evaluation of overall healing, with TRAP(tartrate resistant acid phosphatase) for evaluation of osteoclastic activity and with immunohistochemical staining for macrophages. The results were as follows : 1. In the control group, inflammatory responses were disappeared at day 14, but, in the experimental group inflammatory infiltrates were reduced at day 21. Thus, the experimental group showed more severe soft tissue inflammation than control group. 2. Both control and experimental group showed slight appositional growth at day 7 and gradual bony growth to 21th day. But, complete bony healing of the defect was not shown. There was no significant difference in bony healing between control and experimental group 3. Specific response of macrophages for implanted collagen was observed at day 14 in the experimental group. In conclusion, although fibrillar collagen caused inflammation of soft tissue during initial healing period, inflammatory responses by fibrillar collagen didn't inhibit bony regeneration and implanted collagen was biodegradaded by macrophages. Thus, we expect that fibrillar collagen can be used for useful mediator of graft materials or growth factors.

• Journal of Periodontal and Implant Science
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• 제30권3호
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• pp.527-538
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• 2000

Several extraction cases with advanced bone loss as a result of periodontal disease, root or labial bone fracture, extensive caries, and periapical lesions occur esthetic, functional problems and severe bone loss. Therefore, to treat these cases used several surgical methods and socket preservation among this therapies have been evaluated simple, effective and good prognosis in the implant placement. Socket preservation therapy have been used with barrier membranes or/and graft materials. Deproteinized bovine bone mineral have been evaluated ideal grafting materials. Recently, calcium-phosphate thin film coated bovine bone powders were developed in our country, but the study for these material wasn't reported. When two types of xenograft materials were implanted in extraction sockets of Beagle dogs, the effects of these were analyzed after 4 weeks and 8 weeks histological views. The results of this study were as follows. 1. In control groups, 4 weeks after implantation, the extraction sockets were filled with connective tissue which has dilated vessels and epithelial growth. And after 8 weeks, irregular connective bundles were observed. But new bone formation was not seen. 2. In Bio-Oss groups, epithelial growth was not seen and bone powder was covered with connective tissue fiber. New bone formation was found around the interproximal bone. There was no special change seen after 8 weeks, connective tissue fibers became more regular, and bone growth near bone powder was not made well. 3. In Ca-P BBP groups, epithelial cells didn't grow in the extraction sockets, there was a lot of new bone made around the bone powder after 8 weeks, new bone around bone powder was replaced with mature bone. It is thought that bone powder grafting into the extraction sockets is very useful for conservation of ridge, and Ca-P BBP is more effective in bone formation than Bio-Oss.

• 대한골관절종양학회지
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• 제20권1호
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• pp.1-6
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• 2014

목적: 골육종에서 Connective Tissue Growth Factor (CTGF)의 발현 정도를 확인하고 발암기전에서의 역할을 살펴보고자 하였다. 대상 및 방법: 환자에서 수립한 23개의 골육종 세포주에서 CTGF의 발현 정도를 관찰하였으며, siRNA를 이용하여 CTGF의 발현을 억제한 후 세포침습과 세포 증식에서 CTGF의 역할을 분석하였다. 결과: 17명(74%)의 세포주에서 control 세포인 정상 골모세포보다 CTGF의 발현이 증가되어 있었다. CTGF의 발암기전에서의 역할을 관찰하기 위해 불멸화된 골육종 세포주 SaOS-2와 MG63에서 siRNA로 CTGF의 발현을 억제한 후 siRNA를 transfection한 세포에서 유의하게 세포침습이 억제되고 세포 증식이 억제되는 것을 관찰하였다. 결론: 골육종 세포주에서 CTGF의 발현이 높았고 세포침습, 세포 성장에 관여하는 바 CTGF가 골육종의 발암기전에서 중요한 역할을 하는 것으로 판단된다.

• 폴리머
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• 제25권6호
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• pp.893-901
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• 2001

조직공학적 신경재생 및 파킨슨씨병 등의 시경퇴행성 질환에서의 치료에 이용 목적으로 신경성장인자(nerve growth factor, NGF)를 생분해성 고분자 담체에 NGF를 서방화시키고자 PLA 담체에 함유시켜 유화동결건조법으로 제조하였다. 제조된 NGF의 방출량은 생체외 pH 7.4, 37$^{\circ}C$의 PBS 조건하에서 4주 동안 방출실험 하였으며, 함유된 NGF의 활성을 확인하기 위하여 PC-l2 세포에 직접 배양하여 확인하였다. 제조되어진 PLA 담체는 열린 셀 구조를 가졌으며, 초기 NGF의 함량이 많을수록 방출량도 증가를 보였으며, 제조과정에서의 NGF의 환성을 확인하기 위하여 PC-12 세포를 배양한 결과 신경돌기가 성장하였다. 본 연구는 생분해성 고분자 특정인 확산과 분해에 의해서 생물학적 활성물질인 NGF의 방출을 조절할 수 있으며, 조직공학적으로 서방화되어 3차원적인 신경재생을 가능케 할 것으로 기대된다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제27권5호
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• pp.438-445
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• 2005

Cyclosporine A(CsA) is a powerful immunossuppresive agent used to prevent graft rejection of organ and treat autoimmune disease. One of the major side effects associated with CsA treatment is the development of gingival overgrowth. The purpose of this study was to evaluate the expression and association of the several growth factors in gingival overgrowth induced CsA using by immunohistochemical technique. Normal tissues as control were obtained from healthy normal gingivae and overgrowth gingival tissues as experiments were obtained from the patients taken the CsA. The expressions of the MMP-1, TIMP-1, TGF$\beta$-1, p21, p53, PCNA were evaluated by immunohistochemical technique. The more overgrowth was detected at the epithelial and connective tissue area in experimental group. The MMP-1, TGF$\beta$-1, p21, p53, PCNA expressions were significantly increased in experimental group. The TIMP-1 expressions was not significantly increased in experimental group. We could conclude that the gingival overgrowth induced CsA was related with the collagen matabolism in connective tissue and also the production of the growth factor from epithelial tissue.

• 한국작물학회지
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• 제24권4호
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• pp.62-66
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• 1979

고려인삼의 배 및 식물조직편의 탈분화와 Callus의 유기 및 생장에 미치는 2,4-D 및 Bengyladenine의 효과를 구명하고 Callus와 유기 및 생장에 필요한 2,4-D의 최저 및 최적농도를 밝히고져 본연구를 수행하였던 바 그 결과를 요약하면 다음과 같다. 1. 2,4-D를 0.1mg/l 첨가한 배지상에서는 인나의 배나 식물편에서 Callus가 전혀 유기되지 않았으나 0.5mg/l 처리한 배지상에서는 전체배의 \frac{1}{3} 에서 Callus가 유기되었으며 식물편에서는 모두 약간의 Callus가 유기되었다. 2. Callus의 유기는 2,4-D를 5.0mg/l처리한 배지에서 가장 빨랐으나 Callus의 생장량은 1.0~2.0mg/l의 2,4-D를 첨가한 배지에서 현저히 않은 경향이었다. 3. 2,4-D를 0.5mg/l첨가한 배지에 이식한 배에서는 자엽이 Callus화되어 Callus가 어느 정도 성장한 후 상배축의 기부에서 5~6개이상의 구슬모양의 신아가 형성되었다. 4. Benzyladenine은 근, 경 및 엽의 생장을 억제하고 자엽의 신장비대를 조장하였으며 고농도의 BenByladenine은 Callus의 유기를 억제하는 효과가 현저하였다. 5. 2,4-D 0.1mg/l 및 Benzyladenine 1.0mg/l를 조합처리한 배지상에 이식된 배의 상배축 기부에서도 5개이상의 구슬모양의 신아가 형성되었다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제28권6호
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• pp.421-433
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• 2002

In order to elucidate the pathogenesis of cleft lip and palate, first of all, it is necessary to understand the developmental mechanisms of growth factors and extracellular matrix proteins in the tissues of cleft lip and palate. We have performed immunohistochemical studies on human cleft lip and palate tissues to elucidate the pathogenetic implications of cleft lip and palate. 16 specimens from postnatal human cleft lip and palate subjects and 17 specimens from autopsy of prenatal human cleft lip and palate were fixed in 10% buffered formalin, embedded in paraffin. The sections were routinely stained by hematoxylin and eosin, also stained by PAS, and followed by immunohistochemical stainings using the antiseras of growth factors and extracellular matrix proteins such as PCNA, S-100, c-erb-B2, MMP-3, MMP-10, HSP-70, transglutaninase-C, E-cadherin, VEGF, vWF. Both the prenatal and postnatal specimens of cleft lip and palate showed dysplastic proliferation of the basal cell layer, increased infiltration of melanocytes into mucosal epithelium, sebaceous gland hyperplasia ingrowing into the muscular tissue of lip and palate, and fatty infiltration into the submucosal deep connective tissue. The strong reactions of MMP-3 and HSP-70 were detected in the tissues of cleft lip and palate, especially increased in degenerating muscle bundles, while the immunostainings of PCNA and c-erb-B2 were weakly positive in the tissues of cleft lip and palate. These data suggest that the retrogressive tissue degeneration around the cleft areas persistently exist during the prenatal and postnatal period after cleft formation, and the sebaceous gland hyperplasia and fatty infiltration with the intense expression of MMP-3 and HSP-70 is closely related to the muscular degeneration around the cleft area.