• 생물환경조절학회지
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• 제16권4호
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• pp.358-364
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• 2007

시비농도를 인위적으로 조절하여 잎들깨를 관비재배하면서 인산의 시비수준이 생장과 결핍증상 발현에 미치는 영향을 구명하고, 생육을 우수하게 유지할 수 있는 식물체 및 토양의 한계농도를 밝히기 위하여 본 연구를 수행하였다. 인산이 결핍될 경우 전체 지상부 생육이 심하게 억제되었으며, 노엽에서 초기증상이 발현되고, 엽병과 엽신이 자주색을 띄는 특징을 보였다. 증상이 나타난 부위는 점차 갈변하고 괴사하였다. 본 연구의 인산 시비수준 내에서는 농도가 높아질수록 식물 생육이 증가하여 0, 0.5 및 4.0mM 시비구에서 생체중이 각각 0.48g, 9.289 및 25.5g였고, 건물중이 0.06g, 1.46g 및 4.13g으로 조사되었다. 생육이 가장 우수하였던 4.0mM 처리에서 지상부 인산함량과 엽병추출액의 인산 농도가 1.78% 및 $2,040mg{\cdot}kg^{-1}$였고, 이 보다 10%낮은 식물 생육을 최저 한계점으로 판단한다면 각각 0.3% 및 $900mg{\cdot}kg^{-1}$ 이상의 인산 농도를 유지하도록 시비해야 한다고 판단하였다. 정식 65일 후 인산 4.0mM 처리의 토양 인산 농도가 $1.26mg{\cdot}L^{-1}$였으며, 이 또한 수량감소를 방지하기 위해 $0.57mg{\cdot}L^{-1}$ 이상의 토양 농도를 유지하도록 시비해야 할 것으로 판단하였다.

• 한국균학회지
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• 제27권1호통권88호
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• pp.32-38
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• 1999

양식 가물치로부터 Saprolegnia sp.를 분리하고 그들의 일부 생리학적 특성과 제어를 위한 식물 추출물(精油)을 탐색하였다. 분리된 Saprolegnia sp. 영양체 생장 최적 온도는 $25^{\circ}C$이었고, 최적 pH는 6.0이었으며, 최적 NaCl 농도는 0%이었다. 또한 phosphate 10 mM 농도와 casamino acid 10mg/L농도에서 균사의 생장은 잘되었다. Saprolegnia sp.의 제어 정도를 조사하기 위해 쑥, 편백, 측백나무로부터 정유를 분리하여 10, 100, 500, 1,000, 1,500, 2,000ppm에서 처리한 결과 쑥 정유는 처리농도 10 ppm 이상 농도에서부터 억제 양상이 나타나다가 농도가 높아질수록 더 많은 억제양상을 나타냈다. 측백과 편백나무 정유를 처리한 것에서는 10ppm이상 처리 농도에서부터 억제되기 시작하여 그 이상의 처리 농도에서는 상당한 균사 생장 제어 효과를 나타내다가 500 ppm 이상 처리 농도에서는 완전한 제어 효과를 나타냈다. 또한 Saprolegnia sp.에 감염된 가물치의 조직 병리학적인 조사를 실시한 결과 균사가 감염된 근육조직 부분에는 깊은 곳까지 균사가 침투하였고, 아가미의 세포는 곤봉형태를 나타냈으며, 간조직 세포에서는 일부가 괴사되었고 부분적으로 ceroid 물질이 침착되어 있었다. 또한 비장조직 세포에서는 일부 괴사된 세포를 관찰하였고, ceroid 물질의 침착을 볼 수 있었다. 신장조직은 세뇨관의 상피세포에서 일부 괴사를 관찰하였고, 조혈 조직에는 ceroid 물질의 침착상태가 나타났다.

• Biomolecules & Therapeutics
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• 제23권2호
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• pp.174-179
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• 2015

BP201, porcine lung tissue-derived phospholipids, consists of phosphatidylcholine as a major phospholipid species. BP201 promoted hair growth after application onto the shaved backs of BALB/c and C3H mice. Its effect was enhanced when applied together with minoxidil (MNX) in C3H mice. When the tissue specimens prepared from the shaved skins of BP201-treated and control mice were microscopically examined, the total numbers of hair follicles in both anagen and telogen phases of BP201-treated mice were significantly higher than those of control mice. The numbers of hair follicles in the anagen phase of BP201-treated mice were also higher than those of control mice. In combination with MNX, BP201 further increased the total number of hair follicles, but did not alter the percentage of hair follicles in the anagenic phase. BP201 also increased the proliferation of human hair follicle dermal papilla cells. Collectively, BP201 possesses hair growth promoting potential, which would suggest its use singly or in combination for hair growth products.

• KSBB Journal
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• 제19권3호
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• pp.174-177
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• 2004

기내에서의 발아 및 형질전환 활성을 지닐 수 있는 백합 (Lilium longiflorum) 화분의 장기보존조건을 얻기 위하여 용매를 이용한 연구를 수행하였다. 화분의 용이한 수집을 위하여 용매를 사용했을 때, petroleum ether, n-heptane, benzene 등의 경우 화분의 기내발아에는 영향을 미치지는 않았으나 수집과 정 후 바로 화분건조 및 냉동 보관함으로써 발아활성을 유지 할 수 있었다. 이들의 활성유지는 또한 vacuum infiltration 및 Agrobacterium을 이용한 형질전환에 따른 외래유전자인 tissue plasminogen activator의 발현에 의하여 확인하였다.

• Journal of Dental Anesthesia and Pain Medicine
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• 제17권3호
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• pp.191-198
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• 2017

Background: For peripheral nerve regeneration, recent attentions have been paid to the nerve conduits made by tissue-engineering technique. Three major elements of tissue-engineering are cells, molecules, and scaffolds. Method: In this study, the attachments of nerve cells, including Schwann cells, on the nerve conduit and the effects of both growth factor and adhesion molecule on these attachments were investigated. Results: The attachment of rapidly-proliferating cells, C6 cells and HS683 cells, on nerve conduit was better than that of slowly-proliferating cells, PC12 cells and Schwann cells, however, the treatment of nerve growth factor improved the attachment of slowly-proliferating cells. In addition, the attachment of Schwann cells on nerve conduit coated with fibronectin was as good as that of Schwann cells treated with glial cell line-derived neurotrophic factor (GDNF). Conclusion: Growth factor changes nerve cell morphology and affects cell cycle time. And nerve growth factor or fibronectin treatment is indispensable for Schwann cell to be used for implantation in artificial nerve conduits.

• Asian Pacific Journal of Cancer Prevention
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• 제16권18호
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• pp.8633-8636
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• 2016

Background: To investigate the expression of insulin-like growth factor-I receptor (IGF-IR) and sushi domain containing protein 3 (SUSD3) in breast cancer tissue, and analyze their relationship with clinical parameters and the correlation between the two proteins. Materials and Methods: The expression of IGF-IR and SUSD3 in 100 cases of breast cancer tissues and adjacent normal breast tissues after surgery was detected by immunohistochemical technique MaxVisionTM, and the relationship with clinical pathological features was further analyzed. Results: The positive rate of IGF-IR protein was 86.0% in breast cancer, higher than 3.0% in adjacent normal breast tissue (P<0.05). The positive expression rate of SUSD3 protein was 78.0% in breast cancer, higher than 2.0% in adjacent normal breast tissue (P<0.05). The expression of IGF-IR and SUSD3 was related to estrogen receptor and pathological types (P<0.05),but not with age, stage, the expression of HER-2 and Ki-67 (P>0. 05). The expression of IGF-IR and SUSD3 in breast cancer tissue was positively related (r=0.553, P<0.01). Conclusions: The expression of IGF-IR and SUSD3 may be correlated to the occurrence and development of breast cancer. The combined detection of IGF-IR, SUSD3 and ER may play an important role in judging prognosis and guiding adjuvant therapy after surgery of breast cancer.

• Asian-Australasian Journal of Animal Sciences
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• 제22권4호
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• pp.565-575
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• 2009

Two experiments in the sequential order were conducted to determine the effects of different dietary lipid sources on the growth and fatty acid composition of rohu (Labeo rohita) and to examine the viability of a return fish oil finisher diet in restoring the human cardio-protective fatty acid profile. In the first experiment, fish were fed either with coconut oil (D1), olive oil (D2), sunflower oil (D3), linseed oil (D4) and fish oil (D5) as the main lipid source in the isonitrogenous diet for 90 days. No significant differences in growth were observed. Among the experimental diets moisture content of fish varied significantly (p<0.05) between the groups. Dietary lipid sources had a profound influence on the fatty acid profile of the muscle and liver as tissue fatty acid profile reflected the dietary fatty acid composition. Increased amounts of eicosapentaenoic acid and docosahexaenoic acid were observed in tissue of fish fed D4 and arachidonic acid was observed in the tissue of fish fed D3. We have also detected the metabolites of n-3 and n-6 pathway in D4 and D3 groups respectively, which prompted us to conclude that rohu, can desaturate and elongate $C_{18}$ essential fatty acids to $C_{20}$ and $C_{22}$ HUFA. A second feeding trial was conducted using the animals from the five different treatment groups for the duration of 30 days with fish oil rich diet (D5). Feeding with fish-oil rich washout diet resulted in the near equalization of all the other treatment groups tissue fatty acid profiles to that of fish oil (D5) fed group. These results indicate that a finishing fish oil diet can be effectively used to restore the human cardioprotective fatty acid profile in rohu fed with vegetable oils as lipid source.

• 미생물학회지
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• 제27권2호
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• pp.85-91
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• 1989

조직특이적 및 일반적 유전자 발현에 미치는 SV40와 murine cytomegalovirus (MCMV) enhancer의 영향을 조사하였다. 이를 위하여 chloramphenicol acetyl transferase (CAT) 유전자의 아래쪽에 이들 enhancer들을 삽입한 재조합 플라스미드 들을 제조하였다. 원숭이세포(CV1PO)와 HeLa 세포에 이들 플라스미드들을 이입시킨 후, CAT 유전자가 발현되는 정도를 조사하였다. Enhancer가 없는 플라스미드에 비해 SV40와 MCMV enhancer는 CAT의 발현을 각각 20배와 150배로 가중시켰다. CAT 유전자의 앞에 있는 SV40 프로모터를 2.2kbp의 소성장호르몬(bGH) 유전자의 조절부위로 치환한 경우는 enhancer가 있어도 전혀 CAT 의 말현이 검출되지 않았다. 조절부위을 230 bp 로 짧게 하여 치환한 경우는, SV 40 enhancer 가 있을 때, CAT의 말현이 매우 증가하였다. 이와는 내조적으로 더 강한 MCMV enhancer는 bGH 특이적인 발현을 별로 증가시키지 못하였다.

• Animal Bioscience
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• 제34권7호
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• pp.1134-1145
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• 2021

Objective: This study aimed to investigate the effect of dietary supplementation with Allium mongolicum Regel extracts on the growth performance, carcass characteristics, fat color, and concentrations of three branched-chain fatty acids related to flavor in ram lambs. Methods: Sixty 3-month-old, male, small-tailed Han sheep were selected and randomly allocated into four groups in a randomized block design. Four feeding treatments were used: i) a basal diet without supplementation as the control group (CK); ii) the basal diet supplemented with 10 g/lamb/d Allium mongolicum Regel powder as the AMR group; iii) the basal diet supplemented with 3.4 g/lamb/d Allium mongolicum Regel water extract as the AWE group; and iv) the basal diet supplemented with 2.8 g/lamb/d Allium mongolicum Regel ethanol extract as the AFE group. Results: The results demonstrated that the dry matter intake was lower for the AFE group than that in other groups (p = 0.001). The feed conversion ratio was greater for the AFE than that in other groups (p = 0.039). Dietary supplementation with Allium mongolicum Regel powder and its extracts decreased the concentrations of 4-methyloctanoic acid (MOA) (p<0.001), 4-ethyloctanoic acid (EOA) (p<0.001), and 4-methylnonanoic acid (MNA) (p = 0.044) in perirenal adipose tissue compared to those observed in the CK lambs. Dietary supplementation with Allium mongolicum Regel powder and its extracts decreased the concentrations of MOA (p<0.001) and EOA (p<0.001) in dorsal subcutaneous adipose tissue compared to those in the CK lambs. The concentrations of MOA (p<0.001) and EOA (p = 0.002) in omental adipose tissue were significantly affected by treatment, although there was a tendency for lower MNA (p = 0.062) in AMR, AWE, and AFE lambs than that in CK lambs. Conclusion: This study demonstrated that Allium mongolicum Regel and its extracts could significantly promote feed efficiency, although dry matter intake decreased and could decrease the MOA and EOA concentrations related to characteristic flavor and odor of body fat in lambs, except for tail adipose tissue.

• Journal of Dairy Science and Biotechnology
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• 제34권1호
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• pp.1-7
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• 2016

젖소 초유에는 성장인자가 풍부하게 함유되어 있는데, 상처 치유에 중요한 역할을 하고, 초유의 생리활성 기능을 담당하고 있다. Tyrosine kinase receptor의 활성을 유도하는 성장인자가 특이적으로 관여하여 세포의 분화, 면역기능, 신경기능 등 세포간 상호작용에 관여하는 EGFR(상피증식인자 수용체)와 FGFR(섬유아세포 증식인자)가 있다. 또한 VEGFR (혈관내피 증식인자)와 PDGF(혈소판유래 증식인자)도 존재한다. 조직회복을 위한 각질세포 분화와 세포의 이행에 성장인자가 상승효과를 나타내었고, 초유 또는 초유에 포함된 성장인자 peptide들은 장관질환 치료에 효과가 있으므로 치료제로 이용 가능성을 보여주었다.