• Title/Summary/Keyword: Time-Domain Analysis

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Molecular Cloning of the cDNA of Heat Shock Protein 88 Gene from the Entomopathogenic Fungus, Paecilomyces tenuipes Jocheon-1

  • Liu, Ya-Qi;Park, Nam Sook;Kim, Yong Gyun;Kim, Keun Ki;Park, Hyun Chul;Son, Hong Joo;Hong, Chang Ho;Lee, Sang Mong
    • International Journal of Industrial Entomology and Biomaterials
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    • v.28 no.2
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    • pp.71-84
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    • 2014
  • The full-length heat shock protein 88 (HSP88) complementary DNA (cDNA) of Paecilomyces tenuipes Jocheon-1 was obtained by screening the Paecilomyces tenuipes (P. tenuipes) Jocheon-1 Uni-Zap cDNA library and performing 5' RACE polymerase chain reaction (PCR). The P. tenuipes Jocheon-1 HSP88 cDNA contained an open reading frame (ORF) of 2,139-basepair encoding 713 amino acid residues. The deduced amino acid sequence of the P. tenuipe s Jocheon-1 HSP88 cDNA showed 77% identity to Nectria haematococca HSP88 and 45-76% identity to other fungal homologous HSP88s. Phylogenetic analysis and BLAST program analysis confirmed that the deduced amino acid sequences of the P. tenuipes Jocheon-1 HSP88 gene belonged to the ascomycetes group within the fungal clade. The P. tenuipes Jocheon-1 HSP88 also contained the conserved ATPase domain at the N-terminal region. The cDNA encoding P. tenuipes Jocheon-1 HSP88 was expressed as an 88 kilodalton (kDa) polypeptide in baculovirus-infected insect Sf9 cells. Under higher temperature conditions for the growth of the entomopathogenic fungus, mRNA expression of P. tenuipes Jocheon-1 HSP88 was quantified by real time PCR (qPCR). The results showed that heat shock stress induced a higher level of mRNA expression compared to normal growth conditions.

A Structural Analysis between Overseas Opening of Geospatial Information and the Promotion of Geospatial Information Industry Using the Systems Thinking (시스템 사고를 통한 지도데이터 국외개방과 공간정보 산업 활성화간 인과구조 분석)

  • Yi, Mi Sook
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.36 no.4
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    • pp.213-221
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    • 2018
  • South Korea has been reluctant to open its geospatial information overseas to ensure security as a divided country. However, this cannot continue as the domestic and international environments related to geospatial information and the industrial ecosystem of information and communication technologies have been changing dramatically. Within this context, this study aims to analyze the causal relations among relevant variables and how they change and interact with time using a systems thinking process. First, causal maps were created for the domains of national security, map-based convergence service, and corporate competition. Then, the causal maps for each domain were integrated, based on which the points for policy intervention and dominant feedback loops were identified. The analysis results showed that securing the self-sufficiency of domestic geospatial businesses is a key element to determine the whole causal map, and the variable that changes the dominant feedback loop from a vicious circle to a virtuous one is the decision to open geospatial information overseas. In this study, I found the policy leverage that is a policy intervention point that can produce a great effect with little input by building a causal map of the interactions between major variables. This study is significant in that it identified and analyzed the dominant feedback loop as to which causal structure would dominate the system in the long term. The results of this study can be used to discuss not only the impacts of map data overseas opening on the national security and geospatial information industry, but also the interactions in the future when Google or other global companies request to release the geospatial information.

Application of CFD to Design Procedure of Ammonia Injection System in DeNOx Facilities in a Coal-Fired Power Plant (석탄화력 발전소 탈질설비의 암모니아 분사시스템 설계를 위한 CFD 기법 적용에 관한 연구)

  • Kim, Min-Kyu;Kim, Byeong-Seok;Chung, Hee-Taeg
    • Clean Technology
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    • v.27 no.1
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    • pp.61-68
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    • 2021
  • Selective catalytic reduction (SCR) is widely used as a method of removing nitrogen oxide in large-capacity thermal power generation systems. Uniform mixing of the injected ammonia and the inlet flue gas is very important to the performance of the denitrification reduction process in the catalyst bed. In the present study, a computational analysis technique was applied to the ammonia injection system design process of a denitrification facility. The applied model is the denitrification facility of an 800 MW class coal-fired power plant currently in operation. The flow field to be solved ranges from the inlet of the ammonia injection system to the end of the catalyst bed. The flow was analyzed in the two-dimensional domain assuming incompressible. The steady-state turbulent flow was solved with the commercial software named ANSYS-Fluent. The nozzle arrangement gap and injection flow rate in the ammonia injection system were chosen as the design parameters. A total of four (4) cases were simulated and compared. The root mean square of the NH3/NO molar ratio at the inlet of the catalyst layer was chosen as the optimization parameter and the design of the experiment was used as the base of the optimization algorithm. The case where the nozzle pitch and flow rate were adjusted at the same time was the best in terms of flow uniformity.

Comprehensive analysis of deep learning-based target classifiers in small and imbalanced active sonar datasets (소량 및 불균형 능동소나 데이터세트에 대한 딥러닝 기반 표적식별기의 종합적인 분석)

  • Geunhwan Kim;Youngsang Hwang;Sungjin Shin;Juho Kim;Soobok Hwang;Youngmin Choo
    • The Journal of the Acoustical Society of Korea
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    • v.42 no.4
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    • pp.329-344
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    • 2023
  • In this study, we comprehensively analyze the generalization performance of various deep learning-based active sonar target classifiers when applied to small and imbalanced active sonar datasets. To generate the active sonar datasets, we use data from two different oceanic experiments conducted at different times and ocean. Each sample in the active sonar datasets is a time-frequency domain image, which is extracted from audio signal of contact after the detection process. For the comprehensive analysis, we utilize 22 Convolutional Neural Networks (CNN) models. Two datasets are used as train/validation datasets and test datasets, alternatively. To calculate the variance in the output of the target classifiers, the train/validation/test datasets are repeated 10 times. Hyperparameters for training are optimized using Bayesian optimization. The results demonstrate that shallow CNN models show superior robustness and generalization performance compared to most of deep CNN models. The results from this paper can serve as a valuable reference for future research directions in deep learning-based active sonar target classification.

Prediction of Lifetime of Steel Bridge Coating on Highway for Effective Maintenance (고속도로 강구조물의 효율적 유지관리를 위한 도막수명예측)

  • Lee, Chan-Young;Cheong, Haimoon;Park, Jin-Hwan
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.28 no.3A
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    • pp.341-347
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    • 2008
  • Among coating systems used for steel bridge coatings on highway such as red lead-pigmented alkyd, chlorinated rubber, waterborne inorganic zinc, inorganic zinc/epoxy/urethane and inorganic zinc/epoxy/fluororesin, evaluation of deterioration degree and prediction of lifetime through regression analysis were carried out for coating systems widely used and grossly degraded. For evaluation of deterioration degree, 75 bridges on highway were selected, and evaluations were carried out according to point offering method regulated by Guideline of maintenance coating for steel bridges used in Korea Expressway Corporation. Lifetime prediction results showed 13.0~13.3 years for the whole nation, 11.8 years for urban and industrial region in the metropolitan area, 13.2 years for rural region except the metropolitan area, 13.5~13.7 years for chlorinated rubber coating systems, and 12.86 years for red lead-pigmented alkyd systems. For prediction of the rest life of coating, we tried to execute parallel translations of standard deterioration curve to current life and deterioration degree for both x and y axes, and it was thought that parallel translation for x axis corresponded to deterioration aspects in actual environment. Maximum and minimum equations were derived from standard deterioration equation by adding and subtracting error values deduced in regression analysis to/from each coefficient in order to establish maintenance coating criteria for overall steel bridges on highway. Whole domain was divided into 8 parts in order to predict the rest life of coating and optimum time of maintenance coating, and maintenance coating criteria for each 8 domains were presented.

Measurement and Comparative Analysis of Propagation Characteristics in 3, 6, 10, and 17 GHz in Two Different Indoor Corridors (두 가지 서로 다른 실내 복도에서 3, 6, 10, 17 GHz의 전파 특성 측정 및 비교 분석)

  • Seong-Hun Lee;Byung-Lok Cho
    • The Journal of the Korea institute of electronic communication sciences
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    • v.18 no.6
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    • pp.1031-1040
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    • 2023
  • Propagation characteristics in line-of-sight(LOS) paths in 3, 6, 10, and 17 GHz frequency bands were measured and analyzed in two different indoor corridors: second floors of Buildings D2 and E2. The measurement was designed to measure when the receiving antenna moved at 0.5 m intervals from 3 m to 30 m, while the transmission antenna was fixed. The analysis of the two indoor corridors was compared by applying basic transmission loss, root mean square (RMS) delay spread, and K-factor. For basic transmission loss, the loss coefficient of the floating intercept path loss model was higher in the indoor corridor of Building E2 than in that of Building D2. Similarly, the RMS delay spread in the time domain was greater in the indoor corridor of Building E2. However, the indoor corridor of Building D2 exhibited higher K-factor in the 3, 6, and 17 GHz bands with lower wave propagation in the 10 GHz band. Despite the 2 indoor corridors being identical, the propagation characteristics varied due to different internal structures and materials. The results provide measurement data for ITU-R Recommendations regarding various indoor environments.

A Rational Ground Model and Analytical Methods for Numerical Analysis of Ground-Penetrating Radar (GPR) (GPR 수치해석을 위한 지반 모형의 합리적인 모델링 기법 및 분석법 제안)

  • Lee, Sang-Yun;Song, Ki-Il;Park, June-Ho;Ryu, Hee-Hwan;Kwon, Tae-Hyuk
    • Journal of the Korean Geotechnical Society
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    • v.40 no.4
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    • pp.49-60
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    • 2024
  • Ground-penetrating radar (GPR) enables rapid data acquisition over extensive areas, but interpreting the obtained data requires specialized knowledge. Numerous studies have utilized numerical analysis methods to examine GPR signal characteristics under various conditions. To develop more realistic numerical models, the heterogeneous nature of the ground, which causes clutter, must be considered. Clutter refers to signals reflected by objects other than the target. The Peplinski material model and fractal techniques can simulate these heterogeneous characteristics, yet there is a shortage of research on the necessary input parameters. Moreover, methods for quantitatively evaluating the similarity between field and analytical data are not well established. In this study, we calculated the autocorrelation coefficient of field data and determined the correlation length using the autocorrelation function. The correlation length represented the temporal or spatial distance over which data exhibited similarity. By comparing the correlation length of field data with that of the numerical model incorporating fractal weights, we quantitatively evaluated a numerical model for heterogeneous ground. Consequently, the results of this study demonstrated a numerical modeling technique that reflected the clutter characteristics of the field through correlation length.

A Hybrid Forecasting Framework based on Case-based Reasoning and Artificial Neural Network (사례기반 추론기법과 인공신경망을 이용한 서비스 수요예측 프레임워크)

  • Hwang, Yousub
    • Journal of Intelligence and Information Systems
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    • v.18 no.4
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    • pp.43-57
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    • 2012
  • To enhance the competitive advantage in a constantly changing business environment, an enterprise management must make the right decision in many business activities based on both internal and external information. Thus, providing accurate information plays a prominent role in management's decision making. Intuitively, historical data can provide a feasible estimate through the forecasting models. Therefore, if the service department can estimate the service quantity for the next period, the service department can then effectively control the inventory of service related resources such as human, parts, and other facilities. In addition, the production department can make load map for improving its product quality. Therefore, obtaining an accurate service forecast most likely appears to be critical to manufacturing companies. Numerous investigations addressing this problem have generally employed statistical methods, such as regression or autoregressive and moving average simulation. However, these methods are only efficient for data with are seasonal or cyclical. If the data are influenced by the special characteristics of product, they are not feasible. In our research, we propose a forecasting framework that predicts service demand of manufacturing organization by combining Case-based reasoning (CBR) and leveraging an unsupervised artificial neural network based clustering analysis (i.e., Self-Organizing Maps; SOM). We believe that this is one of the first attempts at applying unsupervised artificial neural network-based machine-learning techniques in the service forecasting domain. Our proposed approach has several appealing features : (1) We applied CBR and SOM in a new forecasting domain such as service demand forecasting. (2) We proposed our combined approach between CBR and SOM in order to overcome limitations of traditional statistical forecasting methods and We have developed a service forecasting tool based on the proposed approach using an unsupervised artificial neural network and Case-based reasoning. In this research, we conducted an empirical study on a real digital TV manufacturer (i.e., Company A). In addition, we have empirically evaluated the proposed approach and tool using real sales and service related data from digital TV manufacturer. In our empirical experiments, we intend to explore the performance of our proposed service forecasting framework when compared to the performances predicted by other two service forecasting methods; one is traditional CBR based forecasting model and the other is the existing service forecasting model used by Company A. We ran each service forecasting 144 times; each time, input data were randomly sampled for each service forecasting framework. To evaluate accuracy of forecasting results, we used Mean Absolute Percentage Error (MAPE) as primary performance measure in our experiments. We conducted one-way ANOVA test with the 144 measurements of MAPE for three different service forecasting approaches. For example, the F-ratio of MAPE for three different service forecasting approaches is 67.25 and the p-value is 0.000. This means that the difference between the MAPE of the three different service forecasting approaches is significant at the level of 0.000. Since there is a significant difference among the different service forecasting approaches, we conducted Tukey's HSD post hoc test to determine exactly which means of MAPE are significantly different from which other ones. In terms of MAPE, Tukey's HSD post hoc test grouped the three different service forecasting approaches into three different subsets in the following order: our proposed approach > traditional CBR-based service forecasting approach > the existing forecasting approach used by Company A. Consequently, our empirical experiments show that our proposed approach outperformed the traditional CBR based forecasting model and the existing service forecasting model used by Company A. The rest of this paper is organized as follows. Section 2 provides some research background information such as summary of CBR and SOM. Section 3 presents a hybrid service forecasting framework based on Case-based Reasoning and Self-Organizing Maps, while the empirical evaluation results are summarized in Section 4. Conclusion and future research directions are finally discussed in Section 5.

Expression profile of defense-related genes in response to gamma radiation stress (방사선 스트레스 반응 방어 유전자의 탐색 및 발현 분석)

  • Park, Nuri;Ha, Hye-Jeong;Subburaj, Saminathan;Choi, Seo-Hee;Jeon, Yongsam;Jin, Yong-Tae;Tu, Luhua;Kumari, Shipra;Lee, Geung-Joo
    • Journal of Plant Biotechnology
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    • v.43 no.3
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    • pp.359-366
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    • 2016
  • Tradescantia is a perennial plant in the family of Commelinaceae. It is known to be sensitive to radiation. In this study, Tradescantia BNL 4430 was irradiated with gamma radiation at doses of 50 to 1,000 mGy in a phytotron equipped with a $^{60}Co$ radiation source at Korea Atomic Energy Research Institute, Korea. At 13 days after irradiation, we extracted RNA from irradiated floral tissues for RNA-seq. Transcriptome assembly produced a total of 77, 326 unique transcripts. In plantlets exposed to 50, 250, 500, and 1000 mGy, the numbers of up-regulated genes with more than 2-fold of expression compared that in the control were 116, 222, 246, and 308, respectively. Most of the up-regulated genes induced by 50 mGy were heat shock proteins (HSPs) such as HSP 70, indicating that protein misfolding, aggregation, and translocation might have occurred during radiation stress. Similarly, highly up-regulated transcripts of the IQ-domain 6 were induced by 250 mGy, KAR-UP oxidoreductase 1 was induced by 500 mGy, and zinc transporter 1 precursor was induced by 1000 mGy. Reverse transcriptase (RT) PCR and quantitative real time PCR (qRT-PCR) further validated the increased mRNA expression levels of selected genes, consistent with DEG analysis results. However, 2.3 to 97- fold higher expression activities were induced by different doses of radiation based on qRT-PCR results. Results on the transcriptome of Tradescantia in response to radiation might provide unique identifiers to develop in situ monitoring kit for measuring radiation exposure around radiation facilities.

A Study on the Effect of Using Sentiment Lexicon in Opinion Classification (오피니언 분류의 감성사전 활용효과에 대한 연구)

  • Kim, Seungwoo;Kim, Namgyu
    • Journal of Intelligence and Information Systems
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    • v.20 no.1
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    • pp.133-148
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    • 2014
  • Recently, with the advent of various information channels, the number of has continued to grow. The main cause of this phenomenon can be found in the significant increase of unstructured data, as the use of smart devices enables users to create data in the form of text, audio, images, and video. In various types of unstructured data, the user's opinion and a variety of information is clearly expressed in text data such as news, reports, papers, and various articles. Thus, active attempts have been made to create new value by analyzing these texts. The representative techniques used in text analysis are text mining and opinion mining. These share certain important characteristics; for example, they not only use text documents as input data, but also use many natural language processing techniques such as filtering and parsing. Therefore, opinion mining is usually recognized as a sub-concept of text mining, or, in many cases, the two terms are used interchangeably in the literature. Suppose that the purpose of a certain classification analysis is to predict a positive or negative opinion contained in some documents. If we focus on the classification process, the analysis can be regarded as a traditional text mining case. However, if we observe that the target of the analysis is a positive or negative opinion, the analysis can be regarded as a typical example of opinion mining. In other words, two methods (i.e., text mining and opinion mining) are available for opinion classification. Thus, in order to distinguish between the two, a precise definition of each method is needed. In this paper, we found that it is very difficult to distinguish between the two methods clearly with respect to the purpose of analysis and the type of results. We conclude that the most definitive criterion to distinguish text mining from opinion mining is whether an analysis utilizes any kind of sentiment lexicon. We first established two prediction models, one based on opinion mining and the other on text mining. Next, we compared the main processes used by the two prediction models. Finally, we compared their prediction accuracy. We then analyzed 2,000 movie reviews. The results revealed that the prediction model based on opinion mining showed higher average prediction accuracy compared to the text mining model. Moreover, in the lift chart generated by the opinion mining based model, the prediction accuracy for the documents with strong certainty was higher than that for the documents with weak certainty. Most of all, opinion mining has a meaningful advantage in that it can reduce learning time dramatically, because a sentiment lexicon generated once can be reused in a similar application domain. Additionally, the classification results can be clearly explained by using a sentiment lexicon. This study has two limitations. First, the results of the experiments cannot be generalized, mainly because the experiment is limited to a small number of movie reviews. Additionally, various parameters in the parsing and filtering steps of the text mining may have affected the accuracy of the prediction models. However, this research contributes a performance and comparison of text mining analysis and opinion mining analysis for opinion classification. In future research, a more precise evaluation of the two methods should be made through intensive experiments.