• The Korean Journal of Mycology
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• v.44 no.2
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• pp.113-117
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• 2016

The biodiversity of endophytic fungi on Thuja koraiensis in Mt. Hwaak, Seorak, and Hambaek, Korea was investigated. For the 202 isolates collected from the host trees, internal transcribed spacer rDNA region sequences-based analysis identified 32 taxa; 61.5% of the isolates belonged to Dothideomycetes, 27.0% belonged to Sordariomycetes, and 11.5% belonged to Leotiomycetes. This composition rate is somewhat different from that reported in previous studies for endophytic fungi inhabiting trees of the family Pinaceae. In particular, Phyllosticta spinarum in Dothideomycetes is a dominant species among the diverse endophytes of T. koraiensis. Therefore, further critical research is required for this species.

• Mycobiology
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• v.50 no.5
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• pp.317-325
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• 2022

Endophytic fungi occupy various ecological niches, which reinforces their diversity. As few studies have investigated the endophytic fungi of alpine conifers, we focused on four species of alpine conifers in this study-Abies nephrolepis, Pinus pumila, Taxus cuspidata var. nana, and Thuja koraiensis-and examined them for endophytic fungi. A total of 108 endophytic fungi were isolated. There were four taxa in A. nephrolepis, 12 in P. pumila, 18 in T. cuspidata var. nana, and 17 in T. koraiensis; these were divided into five classes: Agaricomycetes (3.2%), Dothideomycetes (29.0%), Leotiomycetes (15.0%), Sordariomycetes (41.9%), and Orbiliomycetes (1.6%). The most prevalent fungi were Sydowia polyspora (22.7%) and Xylariaceae sp. (22.7%) in P. pumila, Phomopsis juglandina (16.1%) in T. cuspidate var. nana, and Thuja-endophytes sp. 1 (70.0%) in T. koraiensis. However, there was no dominant species growing in A. nephrolepis. Some host plants were analyzed using next-generation sequencing. We obtained 4618 reads for A. nephrolepis and 2268 reads for T. koraiensis. At the genus level, the top three endophytic fungi were Ophiostomataceae_uc (64.6%), Nectriaceae_uc (15.5%), and unclassified organism (18.0%) in A. nephrolepis and Nectriaceae_uc (41.9%), Ophiostomataceae_uc (41.8%), and Magnaporthaceae_uc (9.2%) in T. koraiensis. Our results show that there are different communities of endophytic fungi among different host plants, even if the host plants are in the same region. Such ecological niches are important in terms of the ecological restoration of alpine conifers.

• The Korean Journal of Mycology
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• v.44 no.4
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• pp.338-341
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• 2016

A dark septate endophytic fungal strain 13E043 was isolated from the needle leaves of Thuja koraiensis from a forest in Korea. Morphological characteristics of conidia and phialids. Along with a molecular phylogenetic analysis based on the internal transcribed spacer region of rDNA, the isolate was identified as Phialocephala piceae (also known as Phaeomollisia piceae). This is the first report of a dark septate endophyte isolated from the foliage of conifer trees in Korea.

• The Korean Journal of Mycology
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• v.43 no.4
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• pp.272-276
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• 2015

We examined endophytic fungi from the needle leaves of three species of conifers in Korea: Abies nephrolepis, Thuja koraiensis, Pinus koraiensis. Endophytic fungi were isolated from the surface-sterilized leaves and identified based on morphological characteristics and Internal transcribed spacer sequences of rDNA. Three species of endophytic fungi isolated in this study were the first reported in Korea: Cryptosporiopsis actinidiae, Pestalotiopsis mangiferae, Pyrenochaeta cava.

• Korean Journal of Plant Resources
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• v.33 no.2
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• pp.93-105
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• 2020

The study was investigated in vegetation and environmental factors of Thuja koraiensis habitats where provided for ecological evidence data for conservation and management. Study plots were located at altitudes of 1,000~1,588 m with inclinations of 10~30°. A total of 150 taxa was identified 22 quadrates in 6 study sites. The highest basal area was analyzed in Mt. Gariwangsan 49.98 ㎡/㏊ with the lowest Mt. Hwaaksan 3.46 ㎡/㏊. Thuja koraiensis forest was classified into two communities; Abeis nephrolepis-Taxus cuspidata community, Betula ermanii-Thuja koraiensis community. As a result of Multi-Response Permutation Procedures test, there was significant difference among the communities.

• Journal of Korean Society of Forest Science
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• v.95 no.4
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• pp.393-397
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• 2006

Thuja koraiensis Nak. is a short and creeping evergreen shrub which reaches about 3 m in height and only occurs in the northeast China and in high mountains over the Korea. It's designated as a rare and endangered tree species in Korea and DD (data deficient) in Red List Category & Criteria of IUCN. This study was carried out to develop the propagation technique by cutting for conservation of genetic resources of T. koraiensis. The rooting responses of branch cuttings, obtained from hard (May) and semi-hard wood shots (August) to three plant growth regulators (PGRs), namely, IAA, IBA, and NAA applied at various concentrations (0, 100, 500, 1000, 2000, and 3000 mg/l) were examined in sand and mixed soil media. Percentage of rooting showed significant difference between cutting time, among kinds and among concentration of PGRs. The optimum cutting time was April to May in hardwood cutting. The application of IAA 1000 mg/l and NAA 500mg/l were effective in callus formation and rooting of cutting. Relatively, rooting of cutting of the control taken in May was above 93%.

• Korean Journal of Environment and Ecology
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• v.24 no.2
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• pp.194-201
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• 2010

This study was carried out to analyze vegetation and soil characteristic, and ordination of subalpine forest vegetation of Daecheongbong area, Mt. Seorak. Communities by characteristic in species composition of subalpine forest were classified into Rhododendron mucronulatum var. ciliatum - Betula ermani community group, Pinus pumila, Abies nephrolepis, Thuja koraiensis, and Rhododendron mucronulatum var. ciliatum - Betula ermanii typical community. The results of the correlation between forest communitie sand soil conditions of Daecheongbong area by DCCA ordination method are as follows: The Pinus pumila community and Thuja koraiensis community were mainly found in the high percentage area of total nitrogen, available phosphorous, organic matter in comparison with Abies nephrolepis community and Rhododendron mucronulatum var. ciliatum - Betula ermanii typical community. The Abies nephrolepis community and Rhododendron mucronulatum var. ciliatum - Betula ermanii typical community were mainly found in the high elevation area in comparison with Pinus pumila community and Thuja koraiensis community. The Pinus pumila community and Thuja koraiensis community were mainly found in the northern and northwestern direction, but Abies nephrolepis community and Rhododendron mucronulatum var. ciliatum - Betula ermanii typical community were mainly found in the southwestern direction.

• Journal of Korean Society of Forest Science
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• v.98 no.1
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• pp.1-7
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• 2009

We investigated the genetic variation and structure in Korean Arborvitae (Thuja koraiensis Nak.), by 29 examining I-SSR polymorphic loci in 84 individuals distributed among four natural populations in Korea. The level of population genetic diversity ($A_e$=1.44, P=72.42, $H_e$=0.258, S.I.=0.385) was similar to or slightly higher than that of plants with similar ecological traits and life history (Cupressaceae). Most genetic diversity was allocated among individuals within populations (${\Phi}_{ST}$=0.13). The UPGMA dendrogram based on genetic distance failed in showing decisive geographic relationship. The Mt. Bangtae population had the lowest level of genetic diversity and was the most distinctive from the other populations. Mt. Jang population which is possessed of the highest level of genetic variation and Mt. Bangtae population which is consisted of heterogeneous was considered to be a prime candidate for the conservation studies.

• Natural Product Sciences
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• v.8 no.4
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• pp.170-172
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• 2002

In continuous efforts for discovery of novel potent antitumor agents from natural products, fifty-seven methanolic extracts derived from indigenous Korean plants were primarily evaluated for in vitro cytotoxic activity in cultured human lung (A549) and colon (Col2) cancer cells. As a result, 16 plant extracts were found to be active against A549 cells and 15 extracts were active against Col2 cells in the criteria of $IC_{50}$<$50\;{\mu}g/ml$. In particular, the extracts of Calystegia soldanella $(IC_{50}$<$8.0\;{\mu}g/ml\;in\;A549;IC_{50}=27.4\;{\mu}g/ml\;in\;Col2)$, Heloniopsis orientalis $(IC_{50}=4.6\;{\mu}g/ml\;in\;A549; IC_{50}=4.5\;{\mu}g/ml\;in\;Col2)$, and Thuja koraiensis $(IC_{50}=1.2\;{\mu}g/ml\;in\;A549;IC_{50}=0.6\;{\mu}g/ml\;in\;Col2)$ showed a potent cytotoxic activity. Further study for the identification of active compounds from these lead extracts might be warranted.

• Journal of the Korean Wood Science and Technology
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• v.34 no.6
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• pp.21-28
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• 2006

This study was carried out to examine the effect of soil improvement according to the shape of charcoal and the kind of carbonized tree species. As the results, all of the seedlings of Thuja occidentalis and Aesculus turbinata grew better in the charcoal-treated soil than the non-treated soil. In case of powder charcoal, Thuja occidentalis seedlings grew up best in the soil treated with powder charcoal of Pinus koraiensis and then grew well in order of Larix leptolepis > particle board > Quercus acutissima. In case of granulated charcoal, the seedlings grew well in order of the granulated charcoal of Larix leptolepis > particle board > Pinus koraiensis. It was analyzed that the soil porosity and the organic matter content were mo re in the charcoal -treated soil than the non-treated soil also. It is inferred that because the aeration property and the absorption of organic matter were increased in the root zone, the growth of seedlings was better in the char-coal-treated soil.