The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Diversity of Endophytes Isolated from Thuja koraiensis Nakai in the Korean Peninsula

눈측백(Thuja koraiensis Nakai)에서 분리한 내생균의 다양성

  • Eo, Ju-Kyeong (Department of Biology Education, Korea National University of Education) ;
  • Lee, Bong-Hyung (Department of Biology Education, Korea National University of Education) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 어주경 (한국교원대학교 생물교육과) ;
  • 이봉형 (한국교원대학교 생물교육과) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Received : 2016.03.30
  • Accepted : 2016.06.20
  • Published : 2016.06.30
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Abstract

The biodiversity of endophytic fungi on Thuja koraiensis in Mt. Hwaak, Seorak, and Hambaek, Korea was investigated. For the 202 isolates collected from the host trees, internal transcribed spacer rDNA region sequences-based analysis identified 32 taxa; 61.5% of the isolates belonged to Dothideomycetes, 27.0% belonged to Sordariomycetes, and 11.5% belonged to Leotiomycetes. This composition rate is somewhat different from that reported in previous studies for endophytic fungi inhabiting trees of the family Pinaceae. In particular, Phyllosticta spinarum in Dothideomycetes is a dominant species among the diverse endophytes of T. koraiensis. Therefore, further critical research is required for this species.

화악산, 설악산, 함백산에 서식하는 눈측백의 잎에서 내생균을 분리하여 다양성을 조사하였다. 숙주식물로부터 총202개의 균주를 분리하였고, 이 균주들의 ITS rDNA 염기서열을 분석한 결과 32개의 분류군으로 동정되었으며, Diaporthe nobilis, Mycosphaerella handelii, Pestalotiopsis cocculi 3종이 국내 미기록종으로 확인되었다. 분리된 균주 중 61.5%는 Dothideomycetes였으며, 27.0%는 Sordariomyetes, 11.5%는 Leotiomycetes에 속하였다. 눈측백에 서식하는 내생균에서 우점종은 Dothideomycetes에 속하는 Phyllosticta spinarum인 것으로 조사되었으며 이 종에 대한 후속연구가 필요한 것으로 생각된다.

Keywords

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