• Mass Spectrometry Letters
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• v.13 no.4
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• pp.158-165
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• 2022

Many therapeutic class drugs such as beta-blocker, corticosteroids, NSAIDs, etc are prohibited substances in the horse racing industry. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology makes it possible to isolate drugs from interference, enables various drug analyses in complex biological samples due to its sensitive sensitivity, and has been successfully applied to doping control. In this paper, we describe a rapid and sensitive method based on solid-phase extraction (SPE) using solid phase cartridge and LC-MS/MS to screen for different class's 35 drug targets in equine plasma. Plasma samples were pretreated by SPE with the NEXUS cartridge consisted non-polar carbon resin and minimum buffer solvent. Chromatographic separation of the analytes was performed on ACQUITY HSS C18 column (2.1 × 150 mm, 1.8 ㎛). The elution gradient was conducted with 5 mM ammonium formate (pH 3.0) in distilled water and 0.1% formic acid in acetonitrile at a flow rate of 0.25 mL/min. The selected reaction monitoring (SRM) mode was used for drug screening with multiple transitions in the positive ionization mode. The specificity, limit of detection, recovery, and stability was evaluated for validation. The method was found to be sensitive and reproducible for drug screening. The method was applied to plasma sample analysis for the proficiency test from the Association of Racing Chemist.

• Fashion & Textile Research Journal
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• v.18 no.3
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• pp.374-379
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• 2016

This study has investigated improvement points of existing products by analyzing problems of horseback riding pants sold in market and by investigating wearing feeling of horseback riders. The purpose of this study is to suggest horseback riding pants which fit to body types of Korean adult women and also have improved functionality by reflecting findings of the study. The contents of this study is believed to suggest useful results on improvement of horseback riding pants sold in market; (1) By visiting hands-on experiencing horseback riding facilities, questionnaire investigation was carried out and we find problems of existing horseback riding pants. (2) Materials, designs and patterns of products sold in market are analyzed for improvement of horse-riding pants appropriate for Korean adult women body types. (3) Based on the results of previous analysis, this study was investigated wearing satisfaction after manufacturing improved horseback riding pants. As results of investigating wearing feeling for products manufactured with pattern designs reflecting the analysis result of existing horseback riding clothes as well as wearing feeling of horseback riders, it was possible to prevent wounds of bodies after horseback riding and the riders expressed high satisfaction for the products manufactured based on the research results.

• Food Science of Animal Resources
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• v.26 no.3
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• pp.375-379
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• 2006

Food labeling regulations require that the meat species in various meat products are accurately declared to the consumer. Substitution or adulteration of costly meat with a cheaper one is one of the most common problems in the meat industry. In this study, PCR-restriction fragment length polymorphism(RFLP) method of the mitochondrial cytochrome b(mt cyt b) gene has been applied for identification of the origin of six mammalian meat species(beef, port horse, goat, mutton and deer) and three poultry meat species(chicken, turkey and duck) as raw materials for meat products. PCR was used to amplify a variable region of mt cyt b gene. Meat species differentiation was determined by digestion of the amplified products with a 359 bp fragment using HaeIII and HinfI restriction enzymes, which generated species-specific RFLP patterns. This PCR-RFLP DNA marker of mt cyt b gene could be very useful for the accurate and reliable identification and discrimination of animal meat species in routine analysis.

• Journal of Veterinary Clinics
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• v.31 no.4
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• pp.357-359
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• 2014

Equine infectious anemia (EIA) is a worldwide infectious disease of horses and other equids. The large serological survey of EIA was performed in Jeju from 2005 through 2011. Using the conventional enzyme-linked immunosorbent assay (cELISA), a total of 10,040 animals (1,329 Jeju Ponies, 8,324 Jeju Pony-Crossbreds and 387 Thoroughbred horses) was tested at the Equine Hospital of Jeju Race Park or Jeju Stud Farm, Korea Racing Authority. This survey found no serological evidence of EIA presence in Jeju. There had been no horse and pony with antibody against EIA since 1985 and no official report on outbreak the disease. Therefore, surveillance conducted found no evidence of EIA activity in Jeju.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.54 no.1
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• pp.12-24
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• 2018

This study was conducted in order to improve opening efficiency of the miniaturized large-scale net for anchovy boat seine gear to reduce the fleet size. Field experiments were performed to observe geometry of nets by catcher boats. When the distances between the two ships were 150, 300 and 450 m, and the speeds of towing nets were 0.6, 0.9, and 1.2 k't, respectively. The vertical opening and actual opening of each part of the miniaturized large-scale net was as follows: the front part of the wing net, 8.7-13.3 m, 51-78%; the middle part of the wing net, 28.1-34.2 m, 55-67%; the entrance of the inside wing net, 31.3-38.5 m, 60-73%; the square and bosom, 22.7-29.6 m, 47-62%; the entrance of the body net, 20.9-26.4 m, 42-52%; the entrance of the bag net, 17.2-21 m, 72-89%; the flapper, 13.2-15.3 m, 78-83%; and the end of the bag net, 13.2-15.7 m, 72-75%. By connecting the net pendants with the front part of the wing net, the opening of the front part of the wing net was significantly improved compared to the traditional gear, which ensured both the wing net and the inside wing net with a normal net height. This, in turn, increased the efficiency of herding. The height of the body and bag nets was also higher than that of the tradition gear. In particular, the body net attached to the gear significantly improved the pocket shape of the gear and reduced the number of fish that were caught and escaped from the bag net, which increased the rate of fishing. The tension of towing nets was measured approximately between 2,958 and 7,110 kg, which indicates that the fleet can tow nets with 350 ps, the standard engine horse power. The fishing operation time was shortened compared with of the existent net, and the large-scale buoy attachment operation was also possible to operate the ship without fish detecting boat.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.56 no.1
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• pp.26-36
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• 2020

Thousands of pelagic and demersal fishes inhabit the waters around Korea and many of them are overexploited. One of the reasons is technological development, which increases the efficiency of the vessels continuously. The analysis was conducted to identify the change of fishing power index to develop the vessel and gear technology that may have improved the fishing efficiency of the otter trawl fishery from 1960s to 2010s. Gross tonnage was decreased stably, but horse power was increased annually. The perimeter of net mouth was somewhat longer, but little changed. Color fish finder was utilized from the mid-1960s. Hydraulic net drum were introduced in the early 1990s, and supply rate was gradually increased. Surveys on the supply and upgrading of fishing equipment utilized visiting research. Therefore, the relative fishing power index in the trawl fishery increased about two to three times in the 2010s compared to the 1980s. The results are expected to contribute to reasonable fisheries stock management.

• Journal of Embryo Transfer
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• v.28 no.4
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• pp.355-360
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• 2013

Precise, rapid and simple methods for species identification in animals are among the most important techniques in the livestock industry and research fields including meat classification. In this study, polymerase chain reaction (PCR) based molecular identification using inter species polymorphisms were examined by PCR-restriction fragment length polymorphism (RFLP) analysis for mitochondrial DNA (mtDNA) cytochrome b (CYTB) gene sequences among four mammalian livestock animals (cattle, horse, goat and pig). The results from PCR-RFLP analysis using the AluI restriction enzyme were also provided for the species-specific band patterns among CYTB gene sequences in these four species. The AluI-digestion for CYTB genes provided interesting migration patterns differentially displayed according to each species. Cattle and horse had one AluI-recognition site at different nucleotide positions and their AluI-digested fragments showed different band patterns on the gels. Pig had two AluI-recognition sites within the amplified CYTB sequences and produced three bands on the gels. Goat had no AluI-recognition site and was located at the same position as the uncut PCR product. The results showed the species-specific band patterns on a single gel among the four livestock animal species by AluI-RFLP. In addition, the results from blind tests for the meat samples collected from providers without any records showed the identical information on the species recorded by observing their phenotypes before slaughter. The application of this PCR-RFLP method can be useful and provide rapid, simple, and clear information regarding species identification for various tissue samples originating from tested livestock species.

• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.4
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• pp.243-246
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• 2018

This study was carried out to identify the effect of the rate of botanical composition, dry matter yield and liveweight gain of horse by renewing low productive pasture. According to the first survey (5.11) before grazing, No.64 was the highest at 81%, followed by No.39 71%t and No.44 65%. For the second and third surveys, the rate of pasture was relatively low, except for No.64. Annual total DM production was the highest at No.44 13,459 kg/ha, then with No.64 and No.39, productivity was 13,232kg/ha and 12,042kg/ha, respectively. No.44 and No.39, the confluence of orchardgrass and perennial ryegrass, showed a sharp decline because of summer depression after the second survey. The livestock growth rate per 10,000 square meters was 70kg, compared with 47.5kg and 36.2kg, respectively. The daily livestock gains in No.39, No.44, and No.66 was 1.09 kg, 1.08 kg and 1.03 kg, respectively.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.55 no.1
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• pp.7-19
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• 2019

This study was conducted in order to improve opening efficiency of the miniaturized small-scale net for anchovy boat seine gear to reduce the fleet size. Field experiment was performed to observe geometry of nets by catcher boats. When the distance between the two ships was 150, 300 and 450 m and the speed of towing nets was 0.6, 0.9, and 1.2 kt, the vertical opening and actual opening of each part of the miniaturized small-scale net was as follows: the front part of the wing net, 6.8-9.5 m, 45-63%; the middle part of the wing net, 16.1-30.7 m, 34-65%; the entrance of the inside wing net, 21.6-41.2 m, 44-84%; the square and bosom, 17.4-34.0 m, 38-75%; the entrance of the body net, 16.5-29.4 m, 36-64%; the entrance of the bag net, 14.5-21.9 m, 70-106%; the flapper, 6.7-7.7 m, 81-83%, and the end of the bag net, 8.6-10.9 m, 64-81%. The tension of towing nets was measured to be 2,734-6,812 kg approximately, which indicates that the fleet can tow nets with 350 hp, the standard engine horse power. The fishing operation time was shortened comparing to existent net with the large-scale buoy attachment operation. It was also possible to operate the ship without fish detecting boat.

• Journal of Soil and Groundwater Environment
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• v.15 no.3
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• pp.44-51
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• 2010

Contamination of groundwater from point and non-point sources is one of major problems of water resource manangement in Jeju island. This study characterized groundwater and soil contamination in Hallim area which is one of the areas of significantly contaminated soil and groundwater in Jeju Island. The amount of loaded contaminant (ALC) of Jeju area was estimated as 13,212 ton N/yr and 3,210 ton P/yr, The ALC of Hallim area was amounted to 2,895 ton N/yr and 1,102 ton P/yr, which accounted for 21.9% and 34.3% of the Jeju's ALC, respectively. The soil pH values (5.6-5.9) were not much different in land use areas. By contrat, average cation exchange capacity (CEC) of 14.1 $cmol^+/kg$ was high comparing to the nationwide range of 7.7-10.9 $cmol^+/kg$. Further, Sodium adsorption ratios (SARs) of horse ranch, pasture, and cultivating land for livestock were as high as 0.19, 0.17, and 0.16 respectively, comparing to the other landuse areas. Nitrate nitrogen at 22.2% of total groundwater wells exceeded 10 mg/L (the criteria of nitrate nitrogen for drinking water), averaginged 6.62 mg/L with maximum 28.95 mg/L. Groundwater types belonged to Mg-$HCO_3$, Na-$HCO_3$, Ca-$HCO_3$, and Na-Cl, among which Mg-$HCO_3$ type occupied more than 70% of the total samples, indicating the presence of anthropogenic sources. The concentration of nitrate nitrogen was negatively related to altitude and well depth, and positively related to the concentration of Ca, Mg, and $SO_4$ which might originate from chemical fertilizer. The ratio of nitrogen isotopes was estimated as an average of 8.10$^{\circ}/_{\circ\circ}$, and the maximum value of 17.9$^{\circ}/_{\circ\circ}$. According to the nitrogen isotope ratio, the most important nitrogen source was assessed as chemical fertilizer (52.6%) followed by sewage (26.3%) and livestock manures (21.1%).