• Title/Summary/Keyword: Test procedures

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Comparison on serological reaction between complement fixation test and enzyme-linked immunosorbent assay for detection of antibodies against Sendai virus, mouse hepatitis virus and Mycoplasma pulmonis in mice and rats (마우스 및 랫트의 Sendai virus, mouse hepatitis Virus, Mycoplama pulmonis 감염(感染)에 대한 보체결합반응(補體結合反應)과 효소표식면역흡착측정법(酵素標識免疫吸着測定法)과의 비교(比較))

  • Chung, Yoo-yeul;Lee, Hak-cheul;Lee, Eun;Yoo, Byung-sam
    • Korean Journal of Veterinary Research
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    • v.29 no.4
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    • pp.517-523
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    • 1989
  • This study was undertaken to establish reliable diagnostic-procedures for the microbiological monitoring of laboratory animals. Murine(mice and rats) antibodies against hemagglutinating virus of Japan(HVJ), mouse hepatitis virus(MHV) and Mycoplasma pulmonis(Mp) were detected sensitively and specifically in experimentally and naturally infected animals' sera by an indirect enzyme-linked immunosorbent assay(ELISA), using urease conjugated antimurine immunoglobulin. The sensitivity and specificity of the complement fixation test which has been apllied widely for serodiagnosis of HVJ, MHV and Mp infections were apparently lower than those of ELISA. From these results, the ELISA was found to be available for the serodiagnosis of HVJ, MHV and Mp infections in mice and rats.

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Flexural properties of a light-cure and a self-cure denture base materials compared to conventional alternatives

  • Mumcu, Emre;Cilingir, Altug;Gencel, Burc;Sulun, Tonguc
    • The Journal of Advanced Prosthodontics
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    • v.3 no.3
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    • pp.136-139
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    • 2011
  • PURPOSE. A new light curing urethane dimethacrylate and a cold curing resin with simpler and faster laboratory procedures may have even improved flexural properties. This study investigated the 3-point flexural strengths and flexural moduli of two alternate base materials. MATERIALS AND METHODS. A cold curing resin (Weropress) and a light curing urethane dimethacrylate base material (Eclipse). Along with Eclipse and Weropress, a high impact resin (Lucitone199) and three conventional base materials (QC 20, Meliodent and Paladent 20) were tested. A 3-point bending test was used to determine the flexural strengths and flexural moduli. The mean displacement, maximum load, flexural modulus and flexural strength values and standard deviations for each group were analyzed by means of one-way analysis of variance (ANOVA) (with mean difference significant at the 0.05 level). Post hoc analyses (Scheffe test) were carried out to determine the differences between the groups at a confidence level of 95%. RESULTS. Flexural strength, displacement and force maximum load values of Eclipse were significantly different from other base materials. Displacement values of QC 20 were significantly different from Lucitone 199 and Weropress. CONCLUSION. The flexural properties and simpler processing technique of Eclipse system presents an advantageous alternative to conventional base resins and Weropress offers another simple laboratory technique.

A Study on the Pants Pattern For the Fatty Body -The Subject of Elementary Schoolboys (비만체형을 위한 바지원형 연구 -학령기 남아를 중심으로 -)

  • 조윤주;윤정혜;박정순
    • Journal of the Korean Society of Costume
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    • v.23
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    • pp.99-110
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    • 1994
  • The purpose of this study was to develop the pattern drafting method which would be suitable for their physical characteristics for the fatty body of Korean elementary school boys who re more than 1.5 Rhrer's Index. The procedures of the study were as follows : We have surveyed the rate of fatty children who were more than 1.5 Rhrer's Index on 2, 185's and elementary school children. The result of research showed the rate of fatty boys in 9-11 years-old were the highest one. Therefore, body measurement and statistical analysis. 203's boys between the ages of 9-11 and over 1.5Rhrer's Index were measured on 25 items. Descriptives, correlation and factor analysis were computed. Fatty boys higher than standard boys, and bigger than grith and width items. 4 type of conventional pants pattern were collected . The most accepted conventional pants pattern was decided through wearing test. The new pattern was based on the most accepted conventional pattern, and developed through wearing test. After new pattern drafting , the sensory evaluation for appearance and comfort was applied to evaluate the new pattern by comparing it with the conventional pattern. The result of sensory evaluation , it was found that the new pattern was more suitable then the conventional pattern.

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Ductile Fracture Behavior of AS4P Under Mixed Mode (I/II) Loading

  • Oh, Dong-Joon
    • Journal of Mechanical Science and Technology
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    • v.16 no.4
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    • pp.476-484
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    • 2002
  • The aim of this study is to investigate the ductile fracture behavior under mixed mode (I/II) loading using SA533B pressure vessel steel. Anti-symmetric 4-point (AS4P) bending tests were performed to obtain the J-R curves under two different mixed mode (I/II) loadings. In addition, finite element analysis using Rousselier Ductile Damage Theory was carried out to predict the J-R curves under mixed mode (I/II) loadings. In conclusions, the J-R curves under. Mixed Mode (I/II) loading were located between those of Mode I and Mode II loading. When the mixity of mixed mode (I/II) loading was high, the J-R curve of mixed mode (I/II) loading approached that of pure mode I loading after some amount of crack propagation. In contrast with the above fact, if the mixity was low, the J-R curve took after that of pure mode II loading. Finally, it was found that the predicted J-R curves made a good agreement with the test data through the tuning procedures of $\beta$ values at the different mixed mode (I/II) loading.

Diagnostic Agents for Oral and Maxillofacial Diseases (구강 질환 진단용 제제)

  • Kho, Hong-Seop
    • Journal of Oral Medicine and Pain
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    • v.24 no.2
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    • pp.181-187
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    • 1999
  • The most important progress in diagnostic sciences is the increased sensitivity and specificity in diagnostic procedures due to the development of newer micromethodologies and increasing availability of immunological and molecular biological reagents. The outcome of researches in this field has already provided DNA probes and antibodies which can be used for diagnosing various kinds of diseases including inherited ones. This development can be also applied to diagnose diseases in oral and maxillofacial regions. Technological advances have yielded highly sensitive test methodologies so that low analyte concentration and small sample volume are no longer limiting factors. Therefore, saliva can be useful test fluid for an array of analytes. Salivary constituents of diagnostic significance include steroid hormones, antibodies, drugs, and tumor markers. Of the proteins present in saliva, viral-specific immunoglobulins are of the greatest diagnostic interest. The development of conjugates and antigens by recombinant DNA technique and peptide synthesis is necessary for clinical application. Several kits developed for the purpose of blood testing should be modified to permit their application to saliva. The final practical outcome of researches in diagnostic sciences will be various diagnostic agents which can be used for detection of bacteria and viruses, screening and diagnosis of diseases, genetic screening for forensic individual identification. For these purposes, collaboration researches and development between institutions and companies are essential.

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Design on Flight-Critical Function of Mission Computer for KUH (한국형기동헬기 임무컴퓨터 비행필수기능 설계)

  • Yu, Yeon-Woon;Kim, Tae-Yeol;Jang, Won-Hong;Kim, Sung-Woo;Lim, Jong-Bong
    • Journal of the Korea Institute of Military Science and Technology
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    • v.14 no.2
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    • pp.213-221
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    • 2011
  • Avionics system tends to be designed to have the integrated architecture, and it is getting difficult and complex to verify the flight-critical function because of sophisticated structure. In Korean Utility Helicopter, mission computer acts as the MUX Bus Controller to handle the data from both communication, identification, mission/display and survivability equipment inside Mission Equipment Package and aircraft subsystems such as fuel system and electrical system while it is interfacing with Automatic Flight Control System and Full-Authority Digital Engine Control via ARINC-429 bus. The Flight Displays which is classified as flight-critical function in aircraft is implemented on Primary Flight Display after mission computer processes data from AFCS in order to generate graphics. This paper defines the flight-critical function implemented in mission computer for KUH, and presents the static and dynamic test procedures which is performed on System Integration Laboratory along with Playback Recorder prior to flight test.

Implementation of Bluetooth Video Distribution Profile Tester based on TTCN

  • Kim, Jae-Youn;Lee, Kang-Hae;Park, Yong-Bum;Lee, Keun-Ku
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • 2009.01a
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    • pp.404-407
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    • 2009
  • Bluetooth Video Distribution Profile (VDP) defines the protocol and procedures that realize the distribution of video content compressed in a specific format for the efficient use of the limited bandwidth. In this paper, we describe the design of VDP tester based on TTCN-2 (Tree and Tabular Combined Notation), a language standardized by ISO for the specification of tests for real-time and communicating systems. Our work was carried out as a part of supporting a new profile testing module for VDP in PTS (Profile Tuning Suite), a reference test system for Bluetooth interoperability testing. Test demonstration for the interoperability with various VDP solutions at the PTS session in UPF30 (Unplug Fest) showed the validity of the developed tester. Eventually, we introduce the PTS architecture, and show the design and implementation of VDP tester included in the released PTS 3.0 in this paper.

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Application of DNA Microarray Technology to Molecular Microbial Ecology

  • Cho Jae-Chang
    • Proceedings of the Microbiological Society of Korea Conference
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    • 2002.10a
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    • pp.22-26
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    • 2002
  • There are a number of ways in which environmental microbiology and microbial ecology will benefit from DNA micro array technology. These include community genome arrays, SSU rDNA arrays, environmental functional gene arrays, population biology arrays, and there are clearly more different applications of microarray technology that can be applied to relevant problems in environmental microbiology. Two types of the applications, bacterial identification chip and functional gene detection chip, will be presented. For the bacterial identification chip, a new approach employing random genome fragments that eliminates the disadvantages of traditional DNA-DNA hybridization is proposed to identify and type bacteria based on genomic DNA-DNA similarity. Bacterial genomes are fragmented randomly, and representative fragments are spotted on a glass slide and then hybridized to test genomes. Resulting hybridization profiles are used in statistical procedures to identify test strains. Second, the direct binding version of microarray with a different array design and hybridization scheme is proposed to quantify target genes in environmental samples. Reference DNA was employed to normalize variations in spot size and hybridization. The approach for designing quantitative microarrays and the inferred equation from this study provide a simple and convenient way to estimate the target gene concentration from the hybridization signal ratio.

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Analysis of Kernel Hardness of Korean Wheat Cultivars

  • Hong, Byung-Hee;Park, Chul-Soo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.44 no.1
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    • pp.78-85
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    • 1999
  • To investigate kernel hardness, a compression test which is widely used to measure the hardness of individual kernels as a physical testing method was made simultaneously with the measurement of friabilin (15KDa) which is strongly associated with kernel hardness and was recently developed as a biochemical marker for evaluating kernel hardness in 79 Korean wheat varieties and experimental lines. With the scattered diagram based on the principal component analysis from the parameters of the compression test, 79 Korean wheat varieties were classified into three groups based on the principal component analysis. Since conventional methods required large amount of flour samples for analysis of friabilin due to the relatively small amount of friabilin in wheat kernels, those methods had limitations for quality prediction in wheat breeding programs. An extraction of friabilin from the starch of a single kernel through cesium chloride gradient centrifugation was successful in this experiment. Among 79 Korean wheat varieties and experimental lines 50 lines (63.3%) exhibited a friabilin band and 29 lines (36.7%) did not show a friabilin band. In this study, lines that contained high maximum force and the lower ratio of minimum force to maximum force showed the absence of the friabilin band. Identification of friabilin, which is the product of a major gene, could be applied in the screening procedures of kernel hardness. The single kernel analysis system for friabilin was found to be an easy, simple and effective screening method for early generation materials in a wheat breeding program for quality improvement.

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Improved Method for Determining the Height of Center of Gravity of Agricultural Tractors

  • Kim, YuYong;Noh, JaeSeung;Shin, SeungYeop;Kim, ByoungIn;Hong, SunJung
    • Journal of Biosystems Engineering
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    • v.41 no.3
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    • pp.170-176
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    • 2016
  • Purpose: This study aimed to improve the method for determining the position of the center of gravity for agricultural tractors. Methods: The proposed method uses trigonometric functions and coordinate transformation. Data were measured according to the ISO 789-6 test procedures for the center of gravity of agricultural tractors. The height calculated using the proposed method was compared with that determined from an AutoCAD drawing. To find the center of gravity of the tractor, the algorithm for finding the intersection of the two lines was used. Results: The vertical height from the ground to the center of gravity is 682.06 mm. The vertical coordinates obtained from the calculation and the drawing were the same. Conclusions: The developed method uses trigonometric and polar coordinate transformation. The method was compared and verified with the AutoCAD drawing results. The results indicate that users can apply this developed method instead of the plotting method which is an inconvenient and time-consuming. Further, users can program Microsoft Excel to easily determine the vertical coordinate. In addition, researchers will propose this method to the ISO as a standard method for determining the center of gravity in accordance with ISO 789-6.