• Title/Summary/Keyword: Test Standard

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Comparison of LAL Test with Pyrogen Test (발열성 물질시험과 세균 내독소 시험의 비교 연구)

  • 이유경;강윤숙;백선영;김용관;신광훈;민홍기
    • YAKHAK HOEJI
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    • v.43 no.5
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    • pp.606-613
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    • 1999
  • To survey the possibility of replacing the pyrogen test with Limulus Amebocyte Lysate(LAL) test and to find out a standard methods suitable to our blood products made in Korea, 100 samples of 20% human serum albumin were tested by commercial LAL test kits and results of those were compared with rabbit pyrogen test. The LAL test is used both dinetic-chromogenically and kinetic-turbidimetrically. Both methods equally showed broad detection range (5.0~0.005 EU/ml), excellent sensitivity ($\geq$ 0.005 EU/ml) and predominant recovery rate within valid dilution range, but kinetic-turbidimetric method seemed to be more reproducible than kinetic-chromogenic method(kinetic-chromogenic method : S.D. = 15.88, kinetic-turbidimetric method : S.D. = 8.12). After heating the sample at 75$^{\circ}C$ for 15 min, the results showed a little elevated recovery rate with both methods. After performing the test on 100 albumin samples with both kits, the results were analysed using the USP standard (1.33 EU/ml). 7% of samples in kinetic-chromogenic methods and 1% of samples in kinetic-turbidimetric method exceeded the limit of endotoxin levels regulated for blood products in USA. Because this phenomenon was not observed in both methods at the same time and both methods have high sensitivity ($\geq$0.005 EU/ml), these results seemed to depend on nonspecific reaction. Considering its sensitivity and reproducibility, we could assure that LAL test is proper to detecting pyrogenic with good sensitivity.

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Comparative Study on the Characteristics of Microalgae as Standard Species for Marine Ecotoxicity Tests (Skeletonema sp., Dunaliella tertiolecta) (해양생태독성시험 표준생물로서 미세조류의 특성 비교 연구(Skeletonema sp., Dunaliella tertiolecta))

  • Kim, Tae Won;Moon, Chang Ho;Lee, Su Jin
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.26 no.5
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    • pp.514-522
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    • 2020
  • To understand the ecotoxicological differences between representative Skeletonema sp. and Dunaliella tertiolecta, both producers as international standard test species for marine ecotoxicity testing, we compared each standard test method, and comparatively analyzed the suitability of the species for environmental assessment and their sensitivity to various test substances. Although most of the test conditions were the same in each method, there were differences in limitation of pH changing and the initial inoculation density in the validation criteria, which is supposed to originate from the low growth rate of D. tertiolecta. In terms of suitability, both species showed consistency in test performance by repeatedly meeting the validation criteria required by the standard test methods. The salinity ranges available for testing were 20 and 10 psu for Skeletonema sp. and D. tertioelecta, respectively. Finally, regarding sensitivity, the toxicity sensitivity of Skeletonema sp. was relatively higher than that of D. tertiolecta for the reference toxicant, actual polluted water discharged (ballast water), and other chemicals. This implies that using at least two species of microalgae from different classification groups could help increase the reliability and objectivity of test results in the performance of marine ecotoxicity tests using producers.

Development of Ecotoxicological Standard Methods using Early Life Stage of Marine Rotifer Brachionus plicatilis and Benthic Copepod Tigriopus japonicus (윤충류 Brachionus plicatilis 및 저서 요각류 Tigriopus japonicus의 초기 생활사를 이용한 해양생태독성시험 방법에 관한 연구)

  • Lee, Seung-Min;Park, Gyung-Soo;Yoon, Sung-Jin;Kang, Young-Shil;Oh, Jeong-Hwang
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.13 no.2
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    • pp.129-139
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    • 2008
  • Marine ecotoxicological standard method was applicated using marine rotifer and benthic copepod as primary consumer of marine ecosystem. Marine rotifer, Brachionus plicatilis and benthic copepod, Tigriopus japonicus were designated as standard test species with the endpoints of 24hr neonate mortality (24hr $LC_{50}$) and 48hr population growth (48hr $EC_{50}$) for rotifer, and 48hr nauplius mortality (48hr $LC_{50}$) for benthic copepod. Tests method was referred to those of ASTM (American Society for Testing and Materials) with the replacement of test species which are widely distributed in Korean waters. The two species showed a wide tolerance on salinity ($5{\sim}35\;psu$) and can be easily cultured in small space even they were not as sensitive as in the mortality test using the nauplius of marine invertebrates. However, these species revealed the significant "concentrationresponse relationship" tested with ocean disposal wastes, and reproducibility using cadmium as standard reference material between laboratories. Accordingly, these two species have good potential as test species for marine ecotoxicological test species. Also, we strongly recommend that at least one of these species be included in the test species of "battery test" in marine bioassay.

A Study on the Automated Compliance Test System for the LSD Protocol Providing the Digital Library Lending Model

  • Kim, Tae-Hyun;Yoon, Hee-Don;Kang, Ho-Gap;Cho, Seung-Hwan
    • Journal of the Korea Society of Computer and Information
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    • v.22 no.4
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    • pp.51-58
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    • 2017
  • Research on the Development of EPUB SCP(Secure Content Protection) Technical Specification and International Standardization has been carried out as a R&D project granted by the Korea Copyright Commission since 2014. The research aims to build a standard framework for the EPUB DRM(Digital Rights Management) based on the Readium LCP(Licensed Content Protection) and KS EPUB DRM specifications, to develop element technologies for the framework, and to standardize it as an IDPF(International Digital Publishing Forum) industrial standard. One of the essential features of the EPUB DRM developed though this study is to support the lending model of the digital library. This paper introduces LSD(License Status Document) specification which is a DRM license management protocol for the digital library lending process, and also represents a model which can automatically test the implemented entities of the LSD specification, test scenarios and test materials.

An Experimental Study on the Bond Characteristics of Glass Fiber Reinforced Polymer Rebar (GFRP Rebar의 부착성능에 관한 실험적 연구)

  • Park Ji-Sun;You Young-Chan;Park Young-Hwan;Choi Ki-Sun;Kim Hyeong-Yeol;Kim Keung-Hwan
    • Proceedings of the Korea Concrete Institute Conference
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    • 2004.05a
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    • pp.124-127
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    • 2004
  • This study is to investigate the bond characteristics of glass fiber reinforced polymer(GFRP) reinforcing bars in concrete by pullout test experimentally. Three different types of GFRP bars with different surface deformations were considered in this study. Also, standard deformed steel reinforcing bar with or without epoxy-coating were included for the comparisons of bond strength. All test procedures including specimens preparation, test apparatus and measuring devices were made according to the recommendation of CSA(Canadian Standards Association) Standard S806-02. From the test results, it was found that small surface indentations contributed to increase the bond strength of GFRP bar significantly. Based on the limited test results till now, the bond strength of GFRP bar with sand-coated deformation commercially available in foreign market is around $80\%$ of that of steel deformed bars.

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A study on Rail Potential Test adaptation for Performance test of DC Railway system (직류 전기철도 성능시험을 위한 레일전위시험 적용연구)

  • Jung, Ho-Sung;Jang, Dong-Uk;Han, Moon-Seob;Park, Hyun-June
    • Proceedings of the KIEE Conference
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    • 2007.10c
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    • pp.238-240
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    • 2007
  • This paper present adaptation method of rail potential test to verify DC railway system's performance and safety. For protection of person and utility caused by rail potential rise, international standard to limit rail potential rise is made and rail potential is tested at the time of plan and construction of DC railway system in foreign country. So, we need rail potential test at the opening time and prepare a continuous monitoring technique of rail potential and a limitation method of rail potential rise. For these, we describe cause of rail potential rise, international standard about rail potential, protection principle, an example of calculation and measurement, and adaptation method for performance test.

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A Study of N-Insertion Preferences in Korean (선호도 조사를 통한 ㄴ첨가 현상의 실현 양상 연구)

  • Kook, Kyungnk-A;Kim, Ju-Won;Lee, Ho-Young
    • MALSORI
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    • no.53
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    • pp.37-60
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    • 2005
  • A Study of N-Insertion Preferences in KoreanKyung-A Kook, Ju-Won Kim, Ho-Young LeeSince n-insertion is not an obligatory process in Korean, it is necessary to investigate what factors influence n-insertion preferences and whether n-insertion preferences have been changed over time. To find answers to these questions, an n-insertion preference test using a questionnaire was conducted. 183 words were selected for this test and 167 subjects participated in the test. The results of this test show that the n-insertion preferences were influenced by the speakers' age, the number and structure of the syllable, word class, phonetic environments, and familiarity. It is suggested that the results of this test should be incorporated into the Principles of Standard Pronunciation and in the Grand Dictionary of Standard Korean.

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An Investigation of Performance Change of Diesel Oxidation Catalyst for Diesel Vehicle (경유차 적용 디젤산화촉매장치의 성능 변화 분석)

  • Hwang, Jin-Woo;Lee, Chang-Sik
    • Journal of ILASS-Korea
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    • v.14 no.1
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    • pp.15-19
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    • 2009
  • This paper is to investigated the analysis of performance characteristics of diesel oxidation catalyst (DOC) for diesel vehicle with 2.5L piston displacement. The performance evaluation test of DOC applied to test diesel vehicle was carried out for four kinds of DOCs manufactured from different company. The testing DOCs were randomly selected from the retrofit vehicle and then standard test vehicle that was representative for the application group was equipped with DOC for the test. In this verification test, the reduction rate of particulate matter (P.M.) and the deviation of the performance of the DOC were examined through CVS-75 mode of the standard vehicle and SOF reduction rate of specific DOC was investigated. It was found that some DOCs failed to pass the criteria of the P.M. reduction rate because of the reason seen catalyst aging even if they were same devices. In the result of SOF analysis, the specific DOC showed more PM reduction than SOF of PM. reduction exceptionally.

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The analysis on the shape of a Standard Test Specimen for the Torsion Test and The Effects of Misalignments (비틀림 시험에 대한 표준시험시편 형상 및 축 정렬 이상 영향 분석)

  • Kim, Ju-Hee;Kim, Yun-Jae;Park, Chi-Yong;Heo, Yong-Hak;Je, Jin-Ho
    • Proceedings of the KSME Conference
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    • 2008.11a
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    • pp.155-160
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    • 2008
  • Using a three-dimensional (3-D) FE analyses, this paper provides the shape optimization of the standard test specimen for the torsion test, as well as a method for analyzing effects of misalignment under the angular and concentric misalignment. For verification, FE analysis is performed, which is designed for the perfectly full-model. To optimize the design shape of the torsion-controlled fatigue test specimen, we performed sensitivity analysis using shape parameters. Additionally, two kinds of misalignment (angular misalignment and concentric misalignment) are applied to the circular and tubular specimens to show effects of misalignments in the FE analysis. The present results will provide valuable information for designing shafts for every kind of mechanical system under torsional force.

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Study on Vibration Fatigue Analysis of Automotive Battery Supporter (자동차 배터리 지지 구조의 진동 피로 해석에 대한 연구)

  • Ah, Sang Ho
    • Journal of Auto-vehicle Safety Association
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    • v.11 no.4
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    • pp.22-27
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    • 2019
  • In this paper, the vibration load and analysis results for automotive battery supporter were performed to provide efficient vibration tolerance performance prediction methods for single-product vibration tolerance testing, and the major influencing factors and considerations for setting up single-unit vibration tolerance tests were reviewed. A common applicable standard load was applied to efficiently predict the performance of single-unit vibrations through the frequency response analysis technique. The results similar to test results can be predicted by checking vulnerable parts of the vehicle components for vibration loads and applying scale factor to standard loads. In addition, it was confirmed that the test conditions with a frequency generating the same durability severity as the endurance test are needed for accurate prediction of the durability of the single-unit vibration tolerance test conditions, and the acceleration and frequency with the conditions that there is no significant nonlinear phenomena in the vibration system are established during the single-unit vibration tolerance test conditions.