• Title/Summary/Keyword: Terminal point

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Responses of Bacteria to TNT: Cells′Survival, SDS-PAGE and 2-D Electrophoretic Analyses of Stress-Induced Proteins (TNT에 대한 세균의 반응기작: 생존율, 스트레스 유도단백질의 SDS-PAGE 및 2-D 전기영동 분석)

  • 오계헌;장효원;강형일;김승일
    • Korean Journal of Microbiology
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    • v.38 no.2
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    • pp.67-73
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    • 2002
  • The cellular responses of soil-borne bacterium, Pseudomonas sp. HK-6 to explosive 2,4,6-trinitrotoluene (TNT) were examined. Two stress shock proteins (SSPs), approximately 70-kDa DnaK and a 60-kDa GroEL were found in HK-6 cells in response to TNT. Analyses of SDS-PAGE and Western blot using anti-DnaK and GroEL revealed that SSPs were induced in HK-6 cells exposed to 0.5 M of TNT far 6-12 hrs. The maximum induction of proteins was achieved at 8-hr incubation point after HK-6 cells'exposure to TNT. Similar SSPs were found to be induced in HK-6 cells by heat shock (shift of temperature, from $30^{\circ}C$ to $42^{\circ}C$) or cold shock (shift of temperature,$30^{\circ}C$ to $4^{\circ}C$).2D-PAGE of soluble protein tractions from the culture of Pseudomonas sp. HX-6 exposed to TNT demonstrated that approximately 450 spots were observed on the silver stained gels ranging from pH 3 to pH 10. Among them, 12 spots significantly induced and expressed in response to TNT were selected and analyzed. Approximately 60-kDa protein, which was assumed highly expressed on the gel, was used for amino acid sequencing. N-terminal microsequencing with in-gel digestion showed that N-terminal sequence of the TNT-induced protein, <$^1XXAKDVKFGDSARKKML^17$, shared extensive similarity with $^1XXAKDVKFGDSARKKML^17$, N-terminal sequence of (P48216) GroEL of Pseudomonas putida.

Light and Scanning Electron Microscopic Study on the Structure of the Gastrointestinal Mucosa of the Mole, Talpa micrura coreana (두더지 (Talpa micrura coreana) 위장관 점막의 구조에 대한 광학 및 주사전자현미경적 연구)

  • Kwun, H.S.;Chung, J.W.;Chun, M.H.;Kim, J.;Cha, J.H.;Maeng, S.H.
    • Applied Microscopy
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    • v.15 no.2
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    • pp.111-129
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    • 1985
  • The morphology and histology of the gastrointestinal mucosa of the mole, Talpa micrura coreana (Thomas), were studied using light and scanning electron microscopes. Tissue specimens were taken from body and pyloric portions of the stomach, and from the initial, proximal, middle, distal and terminal portions of the intestine. For light microscopy, tissue blocks were fixed in 10% buffered neutral formalin, embedded in paraffin wax, and sectioned at a thickness of $5{\mu}m$. These sections were stained with hematoxylin-eosin. For scanning electron microscopy, tissue blocks were fixed in 1% glutaraldehyde-1.5% paraformaldehyde, and postfixed in 1% osmium tetroxide, dehydrated in graded alcohol, transferred to isoamylacetate and dried by the critical point drier(Polaron E 3000). Subsequently, specimens were coated with gold and observed with a JSM-35C scanning electron microscope. The results were as follows: The mucous membrane of the body portion of the stomach had numerous irregular folds and the pyloric mucosa formed the strawberry-shaped folds, and general histological structures of each portion were similar to those of man. The intestine could not be differentiated macroscopically and microscopically into small and large intestines. There was no cecum, appendix, taenia coli, haustra coli or appendices epiploicae. In the initial portion (4 mm long), conical or tongue-shaped villi with the height of $143.3{\pm}10.7{\mu}m$ were present, and large mucous glands were seen in the submucosa. In the proximal, middle and distal portions, wavy folds composed of the epithelium and lamina propria were densely and transversely arranged, and their heights were $440.4{\pm}45.5{\mu}m,\;454.4{\pm}19.9{\mu}m\;and\;205.2{\pm}33.5{\mu}m$, respectively. The mucosa of the terminal portion (3 cm long) formed several longitudinal folds, and the intestinal glands were directly opened on the smooth surface of the folds. Aggregated lymphoid follicles were observed in the major portions of the intestine except the initial and terminal portions. There was no circular or semilunar fold throughout the intestine.

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Design of Miniaturization Terminal Antenna for 2.4 GHz WiFi Band with MZR (MZR을 이용한 2.4 GHz WiFi 대역 소형 단말기 안테나 설계)

  • Lee, Young-Hun
    • Journal of IKEEE
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    • v.23 no.1
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    • pp.14-21
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    • 2019
  • In this paper, we implemented an on-board miniaturization antenna operating 2.4 GHz using MZR(Mu Zero Resonator). It is must be operating under the constraint that the size of the small terminal PCB should be $78{\times}38{\times}0.8mm^3$ and the size of the system should be $63{\times}38{\times}0.8mm^3$ and the size of the radiating part should be $15{\times}38{\times}0.8mm^3$. The feeding structure uses a CPW structure for stable feeding and a feeding point at the upper left of the system board. A magnetic field coupling structure is used for coupling the feeding part and the antenna. The resonance frequency of the MZR is determined by the series inductance and capacitance of the cell, so the gap between the cells, the length of the cell, the length of the interdigital capacitor, and the spacing between the radiation part and the ground plane are analyzed. The antenna was designed and fabricated using the results. The total size of the antenna including the feed structure is $20.8{\times}9.0{\times}0.8mm^3$, and the electrical length is $0.1664{\lambda}_0{\times}0.072{\lambda}_0{\times}0.0064{\lambda}_0$. The measurement result for 10 dB bandwidth, gain and directivity are 440 MHz(18.3%), 0.4405 dB, and 2.722 dB respectively. It is confirmed that the radiation pattern has omnidirectional characteristics and it can be applied to ultra small terminal antenna.

Chilling Requirement for Breaking of Internal Dormancy of Main Apple Cultivars in Korea (국내 사과 주요 품종들의 자발휴면 타파에 필요한 저온요구도)

  • Kweon, Hun-Joong;Sagong, Dong-Hoon;Song, Yang-Yik;Park, Moo-Yong;Kwon, Soon-Il;Kim, Mok-Jong
    • Horticultural Science & Technology
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    • v.31 no.6
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    • pp.666-676
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    • 2013
  • The study was carried out to examine the initial point of dormancy, breaking time of internal dormancy, and to find out the accumulated hours of low temperature (under $7.2^{\circ}C$ from $0.0^{\circ}C$ to $7.2^{\circ}C$) for bud-breaking. Over-all, the chilling requirement for breaking of internal dormancy in the commercial apple cultivars ('Fuji' and 'Tsugaru') and apple cultivars bred in Korea ('Hongro', 'Sunhong', 'Honggeum', 'Hongan', 'Hongso', 'Gamhong', 'Summer dream') at the Gunwi region for 4 years (from 2009 to 2012) was investigated. Also, the breaking time of internal dormancy in the field at the Gunwi region and the breaking time of dormancy if air temperature of Gunwi region rises $4^{\circ}C$ higher than the current one were investigated using the same data. The initial point of dormancy was set at the time when the lateral bud breaking did not occurred (when heading back cutting was done in the middle of terminal shoots). The occurrence of the breaking of internal dormancy was decided if the breaking of the terminal bud of bourse shoot occurred within 15 days or not in growth chamber. About 100 bourse shoots were collected by cultivar classification in early December every year and were stored at $5.0^{\circ}C$, and they were placed in growth chamber at one week interval. The chilling requirement of cultivars was expressed in accumulated hours in the field and in the growth chamber under $7.2^{\circ}C$ and $0.0-7.2^{\circ}C$ from the initial point of dormancy to the breaking time of internal dormancy. The results showed that the initial point of dormancy in selected cultivars could occur at the end of September. The breaking time of internal dormancy could occur from the end of January to the early of February. The accumulated hours under $7.2^{\circ}C$ for breaking of internal dormancy were 1,600-2,000 hours, while those of $0.0-7.2^{\circ}C$ were 1,300-1,800 hours. In comparing the different apple cultivars, the chilling requirement of the early flowering cultivars seemed lower than that of the late-flowering cultivars. Based on these results, if the air temperature of Gunwi region rises about $4.0^{\circ}C$ higher than the current one, the breaking time of internal dormancy will be delayed by 2-4 weeks.

EPON Based Communication Network Architecture for Offshore Wind Power Farm (EPON을 기반으로 한 해상 풍력 단지 통신망 구조)

  • Jung, Jin-Hyo;Yang, Wonhyuk;Kim, Young-Chon
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.38B no.2
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    • pp.130-139
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    • 2013
  • In recent, the communication networks of wind power farm are becoming crucial technologies for monitoring and controlling the large-scale offshore wind power farm. In this paper, EPON based communication network architectures are proposed in order to combat the problems of conventional Ethernet based communication network such as delay, data processing overhead and the fairness of data transmission among turbines in offshore wind power farm. The proposed architecture constructs the point to multi-point network by using OLT and ONUs installed in central control center and wind turbines respectively. The EPON based communication network architecture has low cost, high reliability, and fair transmission capability. To evaluate the performance of proposed architectures, the wind power farm based on IEC 61850 is modelled by using OPNET The simulation results are analyzed and compared with conventional Ethernet based communication network in terms of the overhead, delay and fairness of data transmission.

The POI Model of individualization user central Intelligence Form based of WMS (WMS 기반의 개별 사용자 중심 지능형 POI 서비스 모델)

  • Nam, Haeng-Woo;Kang, Min-Sung;Kim, Bu-Rim;Kim, Eun-Young;Kim, Do-Hyeun;Lee, Sang-Jun
    • Proceedings of the Korea Contents Association Conference
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    • 2006.11a
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    • pp.725-728
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    • 2006
  • Recently, It is gradually increasing about need of various information service based of Location that in compliance with rapidly development wireless internet terminal using the real-time location information at mobile environment. But existing information depends service provision method to information provider. so It is many insufficient tailorable information provision about user individuals each other propensity. For this, It need Service skill to provide easily information about tailorable POI(Point Of Interest) of user preference using information based of Location in mobile computing environment. Therefore In this paper, It is use information service based of location in mobile environment. So It analyzes POI information in compliance with propensity of user and It proposes to provide information about service model. It provides to follow individuals propensity analysis POI information service based of location in proposed model. so It provides actively more value information to user.

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A Study on the Maritime Delimitation Policy of China on Maritime Delimitation in Tonkin Gulf and Policy of Korea (통킹만 경계획정을 통해본 중국의 해양경계획정 정책 및 우리나라 대응방안에 관한 연구)

  • Yang, Hee-Cheol;Park, Seong-Wook;Jeong, Hyeon-Su
    • Ocean and Polar Research
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    • v.29 no.3
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    • pp.245-262
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    • 2007
  • On 25 December 2000, China and Vietnam signed the Agreement on the Delimitation of the Territorial Seas, EEZs and Continental Shelves in the Tonkin Gulf. Three and a half years after signature, in June 2004, China and Vietnam both ratified a maritime boundary agreement for the Tonkin Gulf (Beibu Gulf) and the agreement entered into force. A potentially complicating factor in the negotiation process was likely to have been the status of the Sino-French Agreement of 1887. In the end, the agreement reached indicated that even if the status of the Sino-French Agreement of 1887 was part of the negotiations, both sides eventually agreed that it would not have an impact on the delimitation of maritime zones in the Gulf of Tonkin. Another crucial issue was the impact of the islands, in particular, the Vietnamese controlled Bach Long Vi Island and Con Co Island. Especially, Bach Long Vi Island was entitled to a half suite of maritime zones (3n.m. EEZ) and would impact the tracing of a line of equidistance in the Gulf of Tonkin. Minor as the point might be, Con Co Island also would have an impact for it would play a fixing terminal point for the boundary. Article 7 of the agreement is about minerals and hydrocarbons of cross-boundary deposit, and if any single geophysical structure of oil and gas or other mineral deposits should straddle the demarcation line, an agreement is to be reached on the development of the structure or deposit and on the most effective manner to equally share the profits resulting from the development.

Low Thrust, Fuel Optimal Earth Escape Trajectories Design (저추력기를 이용한 연료 최적의 지구탈출 궤적 설계 연구)

  • Lee, Dong-Hun;Bang, Hyo-Choong
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.35 no.7
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    • pp.647-654
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    • 2007
  • A Discrete continuation Method/homotopy approaches are studied for energy/fuel optimal low thrust Earth escape trajectory by solving a two point boundary value problem(TPBVP). Recently, maneuvers using low thrust propulsion system have been identified as emerging technologies. The low thruster is considered as the main actuator for orbit maneuvers. The cost function consists of a energy/fuel consumption function, and constraints are position and velocity vectors at the terminal escape point. Solving the minimum energy/fuel problem directly is not an easy task, so we adopt the homotopy analysis. Using a solution of the minimum energy, which is solved by discrete continuation method, we obtain the solution of the minimum fuel problem.

Analysis of Wi-Fi Signal Characteristics for Indoor Positioning Measurement (실내 위치 측정을 위한 Wi-Fi 신호 특성 분석)

  • Ha, IlKyu;Zhang, Zhehao;Park, HeeJoo;Kim, ChongGun
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.16 no.10
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    • pp.2177-2184
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    • 2012
  • A different and effective method for indoor positioning system is needed and increased it's importance compare to the outdoor GPS based method. The FingerPrint positioning method is known as a superior method in indoor positioning system that maintains signal strength patterns for RPs(Reference Points) in database and compare the DB with the measured real-time signals on the mobile device. FingerPrint positioning method is necessary to establish an accurate database, but errors can occur by several factors. In this paper, we analyze the signal patterns of each terminal in accordance with connection state of access point and trace that the error in accordance with connection state of access point can be an important error in FingerPrint DB configuration through an experimental case study.

A Study of Call Admission Scheme using Power Strength Threshold value between APs in Wireless LAN Environments (무선랜 환경에서 AP간 전력임계치값을 통한 호 처리 연구)

  • Lim, Seung-Cheol
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.15 no.3
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    • pp.107-112
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    • 2015
  • A smart phone and the number of wireless terminals are mobile-to the nature of the AP mobility are many call transfer between wireless terminals and AP (Access Point). Each wireless terminal that is the call processing process for each is relatively large to cause the wireless traffic, and a factor that inhibits the efficient use of the radio band on the AP. In this paper, we use the power intensity threshold and threshold timer of the AP received by the mobile station to reduce the amount of switching traffic between the AP's cause and traffic generation factors between the wireless device and the AP that can effectively utilize the radio traffic from the AP the measures proposed. The proposed method and the conventional method is improved by simulation to handle the amount of radio traffic from the AP it was confirmed that it is possible to effectively utilize the whole of the radio band.