• ALGAE
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• v.26 no.2
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• pp.153-165
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• 2011

Species of the diatom genus Chaetoceros with special intercalary setae are uncommon. For this study, we collected Chaetoceros species from August 2008 to September 2009 in Korean coastal waters and examined the ultra structures of the Chaetoceros species C. coarctatus, C. compressus var. hirtisetus, C. contortus, C. diversus, and C. messanensis, using light and scanning electron microscopy. C. coarctatus, in the subgenus Phaeoceros, showed longer and stronger spines than those found in other species. C. coarctatus and C. diversus had special intercalary setae with spines in straight arrangements, whereas C. compressus var. hirtisetus, C. contortus, and C. messanensis had special intercalary setae with spines arranged in spirals. The setae of C. coarctatus had spines that were robust toward the tips and, overall, longer and stronger than were those of other species. C. coarctatus and C. diversus were straight, and C. compressus var. hirtisetus, C. contortus, and C. messanensis spiraled. C. messanensis had two types of special intercalary setae, both forked: 1 with spines in spirals and 1 lacking spines. We did not find spines on the anterior part of divergent point of the special intercalary setae of C. messanensis. Foramina shapes of these 5 Chaetoceros species varied as follows: very small or no foramina in C. coarctatus, relatively wide and slightly centrally constricted foramina in C. compressus var. hirtisetus and C. contortus, quite narrowly slitted or no foramina in C. diversus, and lanceolate or hexagonal foramina in C. messanensis. We found rimoportula in both intercalary and terminal valves of C. coarctatus, but C. compressus var. hirtisetus, C. contortus, C. diversus, and C. messanensis only had rimoportula in terminal valves. In addition, C. compressus var. hirtisetus and C. contortus were new to Korean coastal waters.

• The KIPS Transactions:PartC
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• v.8C no.1
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• pp.88-96
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• 2001

Future mobile communication networks, which consist of ATM-based B-ISDN backbone networks and wireless ATM networks, will provide u user with broadband connection and QoS service. These network systems need the lossless handoff methods which support user mobility, satisfy ATM features such as ATM cell ordering and no ATM cell duplication and minimize buffer requirement for ATM cell buffering. In this paper we suggest a multicast-based handoff method to supp$\alpha$t lossless connection as well as to minimize buffer overhead. It establishes a dynamic multicast connection between source terminal and wireless member AP (access point)s. When the mobile terminal receives data packet correctly, it notifies the positive acknowledgement of the packet to all member APs. And member APs then release the MT related temporary buffer space for MT's future movement. Therefore member APs can eliminate unnecessary buffer usage and manage the buffer space efficiently. Analysis result shows that our handoff method has better performance in buffer requirement for lossless and seamless connection services over VCT (virtual connection tree) method and other dynamic multicast-based handoff methods.

• Journal of Advanced Navigation Technology
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• v.20 no.4
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• pp.292-297
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• 2016

Since continuous descent operations (CDO) is one of several tools available to aircraft operators and air navigation service providers (ANSPs) to increase safety, flight predictability, and airspace capacity while reducing noise, controller-pilot communications, fuel burn and emissions, widespread implementation of CDO would result in significant reductions in the environmental impact and aircraft operation costs in south korea as well. After analyzing each procedure from standard terminal arrival routes used for the Incheon international airport, it can be noticed that one of the procedures has a relatively high altitude constraint at initial approach fix than others, which lead the pilots to use unnecessary drag device in certain situations. Therefore we came to a conclusion that some arrival procedures need to be revised, so unnecessary procedure required during approach can be minimized, thereby reducing fuel consumption, noise and emissions compared to current approach procedures. And it is going to increase the safety margin significantly during approach phase due to reduced workload.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.19 no.12
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• pp.2993-3002
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• 2015

INCOTERMS 2010 apply to the International Trade Contract for the last 5 years. The export trade condition and the document type for last 10 years was researched to analysis of the practical problems of INCOTERMS 2010 application. The result of the research; First, several rules which are against the official INCOTERMS 2010 rules are used. Second, the conventional rules like FAS FOB, CIF and CFR which are used for the ocean and inland transportation are applied without any changes. Third, the issue of the time of delivery(transfer of ownership) at the CPT and CIP affects not to activate these two rules. Fourth, the frequency of the DAT use is low. Because, the terminal designate is hard at the point of the contract and the terminal is changeable during the transport. According to these issues, the active publicity campaign is needed for the INCOTERMS 2010. And, the analysis of the terms and the solution of conflict are also needed.

• BMB Reports
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• v.30 no.1
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• pp.46-54
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• 1997

Alkaliphilic Bacillus sp. S-1 secretes a large amount (approximately 80% of total pullulanase activity) of an extracellular pullulanase (PUL-E). The pullulanase exists in two forms: a precursor form (PUL-I: $M_r$ 180,000), and a processed form (PUL-E: $M_r$ 140,000). Two forms were purified to homogeneity and their properties were compared. PUL-I was different in molecular weight, isoelectric point, $NH_2$-terminal amino acid sequence, and stabilities over pH and temperature ranges. The catalytic activities of PUL-I were also distinguishable in the $K_m$ and $V_{max}$ values for various substrates, and in the specific activity for pullulan hydrolysis. PUL-E showed 10-fold higher specific activities than PUL-I. However. PUL-I is immunologically identical to PUL-E, suggesting that PUL-I is initially synthesized and proteolytically processed to the mature form of PUL-E. Processing was inhibited by PMSF, but not by pepstatin, suggesting that some intracellular serine proteases could be responsible for processing of the PUL-I. PUL-I has a different conformational structure for antibody recognition from that of PUL-E. It is also postulated that the translocation of alkaline pullulanase(AP) in the bacterium possibly requires processing of the $NH_2$-terminal region of the AP protein. Processing of the precursor involves a conformational shift. resulting in a mature form. Therefore. precursor processing not only cleaves the signal peptide, but also induces conformational shift. allowing development of active form of the enzyme.

• Journal of Electrical Engineering and Technology
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• v.10 no.4
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• pp.1540-1551
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• 2015

The detection of position and speed in BLDC motors without using position sensors has meant many efforts for the last decades. The aim of this paper is to develop a sensorless technique for detecting the position and speed of BLDC motors, and to overcome the drawbacks of position sensor-based methods by improving the performance of traditional approaches oriented to motor phase voltage sensing. The position and speed information is obtained by computing the derivative of the terminal phase voltages regarding to a virtual neutral point. For starting-up the motor and implementing the algorithms of the detection technique, a FPGA board with a real-time processor is used. Also, a versatile hardware has been developed for driving BLDC motors through pulse width modulation (PWM) signals. Delta and wye winding motors have been considered for evaluating the performance of the designed hardware and software, and tests with and without load are performed. Experimental results for validating the detection technique were attained in the range 5-1500 rpm and 5-150 rpm under no-load and full-load conditions, respectively. Specifically, speed and position square errors lower than 3 rpm and between 10º-30º were obtained without load. In addition, the speed and position errors after full-load tests were around 1 rpm and between 10º-15º, respectively. These results provide the evidence that the developed technique allows to detect the position and speed of BLDC motors with low accuracy errors at starting-up and over a wide speed range, and reduce the influence of noise in position sensing, which suggest that it can be satisfactorily used as a reliable alternative to position sensors in precision applications.

• The Plant Pathology Journal
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• v.25 no.4
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• pp.400-407
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• 2009

A pathogenesis-related protein (PgPR5) gene that isolated from the leaf of Panax ginseng was characterized. The ORF is 756 bp with a deduced amino acid sequence of 251 residues. The calculated molecular mass of the matured protein is approximately 27.5 kDa with a predicated isoelectric point of 7.80. A GenBank BlastX search revealed that the deduced amino acid of PgPR5 shares highest sequence similarity to PR5 of Actinidia deliciosa (80% identity, 87% similarity). PgPR5 has a C-terminal and N-terminal signal peptide, suggesting that it is a vacuolar secreted protein. The expression of PgPR5 under various environmental stresses was analyzed at different time points using real-time PCR. Our results reveal that PgPR5 is induced by salt stress, chilling stress, heavy metal, UV, and pathogen infection. These results suggest that the PgPR5 could play a role in the molecular defence response of ginseng to abiotic and pathogen attack. This is the first report of the isolation of PR5 gene from the P. ginseng.

• Journal of Distribution Science
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• v.16 no.4
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• pp.67-74
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• 2018

Purpose - Most of the amendments to the law on the improvement of the distribution structure of mobile communication terminal equipment, the fully self-sufficient system of terminals, and the separated disclosure system on the terminals are aimed at securing transparency of the distribution structure by eliminating or reducing handset subsidies. This study investigates what items are important for the purchase of mobile phones in various and rapidly changing mobile phone markets from the consumer's point of view and tries to make a strategic suggestion for future mobile distribution strategies. Research design, data, and methodology - The procedure of this study takes place in four steps. In step 1, only the SF type respondents selected for this study were extracted through MBTI analysis. In step 2, they were divided into three hierarchies for the AHP analysis and each element was arranged. In step 3, the AHP analysis was converted to a Fuzzy-AHP number using the trigonometric centroid method. This was to eliminate the ambiguity of the response by converting into a fuzzy number even if data consistency was maintained with CI value below 0.1. In step 4, the number of converted 2-layer and 3-layer was combined to derive the priority when the final handset is selected. Results - First, the highest importance among the four items in the second tier was the terminal function item, followed by brand, price, and design item. Second, in the third tier, the highest importance was level of after-sales service, followed by device price, processing speed, ease of use, usefulness, and rate system. Third, the arithmetic average of the determinant of the fuzzy function showed that processing speed, ease of use and usefulness in the function item, level of after-sales service in the brand item, and device price in the price item were the five most important factors among 16 choice factors. Conclusions - First, there will be a change in the consumption patterns of consumers who have compared distributors and dealers to purchase handsets with more subsidies. Second, it is highly likely that people will purchase new handsets only when they need to change their devices because they can not receive subsidies by switching phone brands any more.

• Molecules and Cells
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• v.37 no.2
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• pp.140-148
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• 2014

We identified four basic amino acid residues as nuclear localization signals (NLS) in the C-terminal domain of the prototype foamy viral (PFV) integrase (IN) protein that were essential for viral replication. We constructed seven point mutants in the C-terminal domain by changing the lysine and arginine at residues 305, 308, 313, 315, 318, 324, and 329 to threonine or proline, respectively, to identify residues conferring NLS activity. Our results showed that mutation of these residues had no effect on expression assembly, release of viral particles, or in vitro recombinant IN enzymatic activity. However, mutations at residues 305 (R ${\rightarrow}$ T), 313(R ${\rightarrow}$ T), 315(R ${\rightarrow}$ P), and 329(R ${\rightarrow}$ T) lead to the production of defective viral particles with loss of infectivity, whereas non-defective mutations at residues 308(R ${\rightarrow}$ T), 318(K ${\rightarrow}$ T), and 324(K ${\rightarrow}$ T) did not show any adverse effects on subsequent production or release of viral particles. Sub-cellular fractionation and immunostaining for viral protein PFV-IN and PFV-Gag localization revealed predominant cytoplasmic localization of PFV-IN in defective mutants, whereas cytoplasmic and nuclear localization of PFV-IN was observed in wild type and non-defective mutants. However sub-cellular localization of PFV-Gag resulted in predominant nuclear localization and less presence in the cytoplasm of the wild type and non-defective mutants. But defective mutants showed only nuclear localization of Gag. Therefore, we postulate that four basic arginine residues at 305, 313, 315 and 329 confer the karyoplilic properties of PFV-IN and are essential for successful viral integration and replication.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2006.11a
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• pp.335-338
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• 2006

The more deeply the researches make progress in ocean researches including the seabed resource investigation or the oceanic ecosystem investigation, the more important the role of UUV gets. In case of study on the deep sea, there are difficulties in telecommunications between AUV and ships, and in data communication and recharging. Therefore, docking is required. In AUV docking system, the AUV should identify the position of docking and make contact with a certain point of docking device. MOERI (Maritime & Ocean Engineering Research Institute), KORDI has conducted the docking testing on AUV ISIMI in KORDI Ocean Engineering Water Tank. As AUV ISIMI approachs the docking device, it is presented that attitude is unstable, because the lights Which is on Image Frame are disappeared. So we fix the rudder and stem, if the lights on Image Frame are reaching the specific area in the Image Frame. In this paper, we intend to solve the problems that were found in the testing, which, first, will be identified via simulation.