• 대한치과보철학회지
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• 제36권2호
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• pp.245-257
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• 1998

This study was performed to measure the changes of the mandibular movement and the masticatory muscular activities - anterior temporal and masseter muscle of both side - reflected by intentional increase of anterior guidance angie. For this study, 5 volunteers (3 males and 2 females with average age of 24.0) were selected. Each volunteer had Angle's classification I and did not have any missing tooth except third molar and any extensive restorations. Metallic guide plate was made at volunteer's working model fabricated by improved dental stone and cemented to the palatal surface of maxillary central incisor using resin cement(Panavia $21^{(R)}$) and then adjusted not to give any occlusal interferences at intercuspal position. The activity of masticatory muscles and the changes of mandibular movement were recorded by EMG and Sirognathograph in Biopak analysing system(Bioresearch Inc., Milwaukee, Wisconsin, USA). Measurement was done at before experiment, immediatley after placement, 1 week after placement, immediately after removal, and 1 week after removal. The results were as follows: 1. Moderate phonetic disturbance and mild headache were occured to 3 volunteers for 2 days after setting and 1 volunteer had positive reaction to percussion and slight midline diastema. But all of these clinical signs were diappeared 1 week after removal and the other volunteer did not have any special clinical sign. 2. In the EMG of the mandibular rest position, the mean value of anterior tempotal muscle was increased immediately after placement(p<0.01) and then decreased 1 week after placement(p<0.05) and increased 1 week after removal(p<0.05) but not recovered as before experiment. The mean value of masseter muscle was decreased during the experiment period. 3. In the EMG during mandibular protrusive movement, all muscular activity was decreased during the experiment period. Reduced activity was not recovered 1 week after removal(p<0.03). 4. During the habitual opening, anteroposterior movement of mandible was decreased immediately after placement(p<0.05) and then increased 1 week after placement but not statistically significant(p>0.1). Vertical movement was not shown significant difference during the experiment period(p>0.1). Lateral movement was decreased immediately after placement(p<0.05) and then increased 1 week after placement but not recovered as before experiment. The opening and closing velocity of mandible was shown minor changes but not statistically significant. 5. During the habitual opening, anteroposterior movement of mandible was decreased 1 week after placement(p<0.05) and then increased immediately after removal and recovered 1 week after removal as before experiment. Vertical movement was not shown significant changes. Lateral displacement of mandible was increased continuously and recovered 1 week after removal. Opening velocity was temporarily increased immediately after removal but recovered and closing velocity was not shown significant changes. 6. During the right side chewing, anteroposterior movement of mandible was increased immediately after removal but recovered and vertical movement was not shown statistically significant results. Lateral displacement and velocity of mandible were not shown significant results. 7. During the left side chewing, the changes of mandibular movement pattern were not shown statistically significant results.

• 한국대기환경학회지
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• 제22권5호
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• pp.642-652
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• 2006

In order to understand the characteristics of surface ozone concentration and high $O_3$ concentration days, regional data from seven air quality monitoring stations which were operated by local governments were analyzed Regional characteristics of $O_3$ concentration were analyzed with the data of $O_3$ concentration and the characteristics of $O_3$ generation and weather conditions by the selection of the days in which the concentration was higher than 80 ppb. In the case of daily variation, the lowest $O_3$ concentration was shown in all regions from 7am to 8am and the highest around at 4 pm. The monthly variation of mean $O_3$ concentration and ${\Delta}O_3$ values revealed a reducing pattern in July and August following the peak in June, and again a gradual increasing trend in September and October. The result shows that the amount of ozone is dependent on photochemical reaction. The days of $O_3$ generated more than 80 ppb in the region of Gwangyang-bay were 544 days(1,760 hrs). The frequency of occurrence in the region revealed a strong pattern with the order of Samil-dong, Jinsang, and Gwangmu-dong stations in the Gwangyang region. However, Tein-dong, which is the nearest station to air pollution material generation source, showed the lowest frequency in the study area. Consequently, the meteorological parameters which can easily generate the high concentration of $O_3$ in the region of Gwangyang-bay are characterized as follows; atmospheric temperature which is higher than $19^{\circ}C$, relative humidity with the range of $60{\sim}85%$, the less average wind velocity than 5 m/s, cloud cover which is less than 5/10, and the more duration of sunshine than 8 hours.

• 대한통합의학회지
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• 제9권2호
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• pp.83-92
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• 2021

Purpose : Keratinocytes are the main cellular components involved in wound healing during re-epithelization and inflammation. Dysfunction of tight junction (TJ) adhesions is a major feature in the pathogenesis of various diseases. The purpose of this study was to identify the various effects of a Sparassis crispa water extract (SC) on HaCaT cells and to investigate whether these effects might be applicable to human skin. Methods : We investigated the effectiveness of SC on cell HaCaT viability using MTS. The antioxidant effect of SC was analyzed by comparing the effectiveness of ABTS to that of the well-known antioxidant resveratrol. Reverse-transcription quantitative polymerase chain reaction (qRT-PCR) is the most widely applied method Quantitative RT-PCR analysis has shown that SC in HaCaT cells affects mRNA expression of tight-junction genes associated with skin moisturization. In addition, Wound healing is one of the most complex processes in the human body. It involves the spatial and temporal synchronization of a variety of cell types with distinct roles in the phases of hemostasis, inflammation, growth, re-epithelialization, and remodeling. wound healing analysis demonstrated altered cell migration in SC-treated HaCaT cells. Results : MTS analysis in HaCaT cells was found to be more cytotoxic in SC at a concentration of 0.5 mg/㎖. Compared to 100 µM resveratrol, 4 mg/㎖ SC exhibited similar or superior antioxidant effects. SC treatment in HaCaT cells reduced levels of claudin 1, claudin 3, claudin 4, claudin 6, claudin 7, claudin 8, ZO-1, ZO-2, JAM-A, occludin, and Tricellulin mRNA expression by about 1.13 times. Wound healing analysis demonstrated altered cell migration in SC-treated HaCaT cells and HaCaT cell migration was also reduced to 73.2 % by SC treatment. Conclusion : SC, which acts as an antioxidant, reduces oxidative stress and prevents aging of the skin. Further research is needed to address the effects of SC on human skin given the observed alteration of mRNA expression of tight-junction genes and the decreased the cell migration of HaCaT cells.

• 환경분석과 독성보건
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• 제21권4호
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• pp.220-228
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• 2018

$PM_{2.5}$-bound mercury (PBM) was monitored at weekly intervals for three years (from 2014 to 2016) at an urban (Seoul) and rural site (Baengnyeong) in South Korea. The average PBM concentrations in $PM_{2.5}$ samples over the entire sampling period were $12{\pm}11pg/m^3$ and $36{\pm}34pg/m^3$ for Baengnyeong and Seoul, respectively. Seasonal differences were pronounced, with concentrations being highest in winter due to local meteorological conditions (high gas-particle coefficient due to low temperature and low mixing layer height in winter) as well as seasonal factors, such as coal combustion for heating purposes in China. In Baengnyeong, the significant positive correlation of PBM with $PM_{2.5}$, air pollutants, and heavy metals suggested that coal combustion in China might be the most important source of ambient mercury in Korea. In winter, no correlation of PBM with $PM_{2.5}$, air pollutants, and heavy metals was seen in Seoul. Furthermore, Seoul showed higher $PBM/PM_{2.5}$ and $Pb/PM_{2.5}$ ratios in winter due to the strong atmospheric oxidation-reduction reaction conditions as well as local and regional PBM sources. We conclude that immediate attention must be given to addressing PBM levels in Korea, including considering it as a key component of future air quality monitoring activities and mitigation measures.

• 한국지구과학회지
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• 제32권3호
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• pp.276-293
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• 2011

본 연구에서는 한반도 황사 사례 동안 WRF 기상모델과 SMOKE 배출량모델, CMAQ 및 CMAQ-MADRID 대기질 모델을 이용하여 다양한 황사 발생량 경험식에 대한 $PM_{10}$의 농도를 추정하였다. 특별히 Wang et al.(2000), US EPA 모델, Park and In(2003), GOCART 모델, DEAD 모델의 5가지 황사 발생 경험식이 중국과 몽골 등의 황사 발생량을 추정하기 위해 WRF-SMOKE-CMAQ(MADRID) 모델에 적용되었다. 일기도, 후방궤적 및 위성이미지 분석에 따르면 한반도로의 황사 수송은 절리저기압(위성에서 콤마형 구름)과 관련된 지상 전선의 뒤쪽에서, 그리고 상층 제트류의 발달에 기인한 파의 정체현상과 함께 상층 골에서의 풍속이 하층으로 전이되는 풍하 바람에 의해 생성되었다. 그리고 WRF-SMOKE-CMAQ 모델링 결과, 황사의 시 공간적 분포에 있어서는 Wang et al.(2000)의 경험식이, 평균 편의 및 평균 제곱근 오차에서의 정확도 부분에서는 GOCART 모델의 경험식이 관측값을 보다 잘 모사하는 것으로 나타났다. 또한 Wang et al.의 경험식을 이용한 황사의 연직분포 분석 결과에서 강한 황사 사례(2007년 3월 31에서 4월 1일 $800\;{\mu}g/m^3$ 이상)의 경우는 황사 수송이 한반도 상공 대기 경계층 내를 통과하였기 때문으로, 약한 황사 사례(2009년 3월 16일과 17에 $400\;{\mu}g/m^3$ 이하)의 경우는 황사 수송이 경계층 위를 통과하였기 때문으로 나타났다. 또한 CMAQ 모델과 CAMQ-MADRID 모델에서의 미세먼지($PM_{10}$) 민감도 분석 결과에서는 CMAQ-MADRID 모델이 CMAQ 모델에 비해 한반도를 포함한 동아시아 지역에서 최대 $25\;{\mu}g/m^3$ 정도가 높게 모사되었고, 모델 내 구름 액상과정에 의해서는 최대 $15\;{\mu}g/m^3$ 정도가 제거되는 것으로 나타났다.

• 인지과학
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• 제27권4호
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• pp.573-597
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• 2016

최근 행위 개념의 신경적 표상에 대한 fMRI나 EEG 연구들은 행위 개념의 처리가 감각-운동 정보의 모사(simulation)를 불러일으킨다고 주장한다. 이와 아울러 행위동사나 행위 문장 이해가 현재 수행해야 하는 행위를 간섭하거나 촉진시킨다는 행동연구들도 존재한다. 그러나 행위 개념 처리와 현재 행동 수행간의 실시간 상호작용이 감각-운동정보의 모사를 매개로 하는지, 또 다른 기전에 바탕을 두는지 아직까지 분명하지 않다. 본 연구에서는 행위 언어 지각이 어떻게 현재 행위 수행에 영향을 주는지 그 기저에 있는 신경 메커니즘을 시공간적으로 탐색하고자 시간해상도가 높은 뇌파 측정과 다중전류원분석이라는 뇌파분석 기법을 사용하였다. 이를 위하여 실험참가자에게 단서 자극 색상에 따라 손으로 버튼 누르기 행동과 발로 페달 밟기 행동을 해야 하는 단서-운동반응 과제를 수행하도록 하였고, 단서가 제시되기 직전에 반응 행위자체를 기술하는 행위동사(즉, 눌러라, 밟아라, 멈춰라)를 청각적으로 제시하여 행위동사와 반응 행동 간의 의미 일치성에 따른 상호작용을 관찰하였다. 반응시간 분석 결과, 손으로 버튼을 누르는 반응행위와 발을 이용하여 페달을 밟는 반응행위 모두에서 행위동사와 반응행위가 일치하는 경우 촉진효과가 관찰되었고, 불일치에 따른 간섭효과는 손 행위에서 나타났다. 전류원 파형 분석결과, 행위동사와 반응행위간의 의미일치성 효과는 행위 동사 처리 기간에는 베르니케 영역, 운동단서 제시 시점에선 전대상회와 보조운동영역, 운동수행 시점에서는 보조운동영역과 일차운동피질에서 통계적으로 유의한 차이가 관찰되었다. 현재 결과로는 행위동사가 특정 운동피질을 활성화시켜 이를 매개로 현재 행위에 영향을 준다고 하기보다, 뒤따르는 행위를 예측하고 이를 준비하는 과정에 영향을 미쳐, 촉진/간섭효과를 가져오는 것으로 보인다. 마지막으로 본 연구가 가지는 행위 개념의 신경학적 표상에 대한 함의와 연구 방법론상 한계에 대해 논의하였다.

• Journal of Plant Biotechnology
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• 제30권1호
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• pp.13-18
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• 2003

본 실험은 감자괴경에 특이적으로 나타나는 patatin promoter에 의한 외부 도입 유전자의 발현 양상을 파악하고자 수행되었다. Patatin promoter에 의하여 GUS 유전자의 발현이 조절되도록 pATGUS 벡터를 제작한 후 Agrobacterium tumefaciens LBA4404를 이용하여 감자의 잎 절편에 형질전환하였다. 대조구로 GUS 유전자의 상시발현 벡터인 pBI121을 사용하였으며, 항생제를 포함한 재분화 배지에서 개체를 유도한 결과 8주 후부터 신초를 관찰할 수 있었다. NPTII 유전자의 삽입여부를 PCR로 검정한 후, 선별된 형질전환체의 소괴경 형성을 위해 sucrose 농도를 높인 배지에서 1주일 간격으로 줄기의 하단 부분을 배양하였다. 주별로 시료를 채취한 후, RNA gel blot 분석을 해 본 결과 CaMV35S promoter에 의한 GUS 발현은 소괴경의 전단계에서 고르게 발현되는 반면, 괴경-특이적인 patatin promoter의 경우 감자 줄기에서는 관찰이 어려웠고, 주별 발현율은 1주부터 5주까지는 점점 증가하다가 그 이후부터는 점차 감소함을 알 수 있었다. 또한, GUS의 효소활성 역시 mRNA의 발현율과 비례함을 알 수 있었다. 제시된 실험결과들로 보아 감자 괴경에서의 patatin promoter에 의한 GUS 유전자의 발현은 5주경에 가장 높게 나타났으며, 이러한 결과들로 보아 patatin promoter에 의해 감자 소괴경내로 도입된 외래 유전자의 발현을 확인하기 가장 좋은 시기는 소괴경 형성 후 5주째임을 알 수 있었다.