• Title/Summary/Keyword: Technology Development Project

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Current status of Ac/Ds mediated gene tagging systems for study of rice functional genomics in Korea (Ac/Ds 삽입 변이체를 이용한 벼 유전자 기능 연구)

  • Lee, Gang-Seob;Park, Sung-Han;Yun, Do-Won;Ahn, Byoung-Ohg;Kim, Chang-Kug;Han, Chang-Deok;Yi, Gi-Hwan;Park, Dong-Soo;Eun, Moo-Young;Yoon, Ung-Han
    • Journal of Plant Biotechnology
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    • v.37 no.2
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    • pp.125-132
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    • 2010
  • Rice is the staple food of more than 50% of the worlds population. Cultivated rice has the AA genome (diploid, 2n=24) and small genome size of only 430 megabase (haploid genome). As the sequencing of rice genome was completed by the International Rice Genome Sequencing Project (IRGSP), many researchers in the world have been working to explore the gene function on rice genome. Insertional mutagenesis has been a powerful strategy for assessing gene function. In maize, well characterized transposable elements have traditionally been used to clone genes for which only phenotypic information is available. In rice endogenous mobile elements such as MITE and Tos (Hirochika. 1997) have been used to generate gene-tagged populations. To date T-DNA and maize transposable element systems has been utilized as main insertional mutagens in rice. A main drawback of a T-DNA scheme is that Agrobacteria-mediated transformation in rice requires extensive facilities, time, and labor. In contrast, the Ac/Ds system offers the advantage of generating new mutants by secondary transposition from a single tagged gene. Revertants can be utilized to correlate phenotype with genotype. To enhance the efficiency of gene detection, advanced gene-tagging systems (i.e. activation, gene or enhancer trap) have been employed for functional genomic studies in rice. Internationally, there have been many projects to develop large scales of insertionally mutagenized populations and databases of insertion sites has been established. Ultimate goals of these projects are to supply genetic materials and informations essential for functional analysis of rice genes and for breeding using agronomically important genes. In this report, we summarize the current status of Ac/Ds-mediated gene tagging systems that has been launched by collaborative works from 2001 in Korea.

A Study on the Component Design for Water Network Analysis (상수도 관망해석 컴포넌트 설계에 관한 연구)

  • Kim, Kye-Hyun;Kim, Jun-Chul;Park, Tae-Og
    • Journal of Korea Spatial Information System Society
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    • v.2 no.2 s.4
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    • pp.75-84
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    • 2000
  • GIS has been building for various application fields with the aids of NGIS project, especially numerous municipal governments are building a UIS in the level of local governments' informatization. Although there are some difference between municipal governments' business, still many things are in common. So far, individual municipal governments have developed a UIS for their own use, which lead to duplicated development of the UIS. The component technology has been introduced to remove such duplicated efforts and it enabled maximizing the reusablilty of the UIS already developed. This paper proposes a component design for network analysis of the drinking water to calculate the amount of flow and the head loss. This component design provides the initial water amount to estimate the amount of the network flow and the head loss, thereby supports the decision making such as installation or extension of the pipe network. The process of the component design accompanies the business reengineering to support the standardized business work flow. Also, the design of the network analysis component uses the algorithms induced with UML specification. Based on the component design, the component development has been progressing and the network analysis system would be followed. In the near future, another component to integrate the network analysis and the business related to the drinking water needs to be developed.

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Development Trend of Korean Staged Combustion Cycle Rocket Engine (한국형 다단연소사이클 로켓엔진 개발 동향)

  • Kim, Chae-hyoung;Han, Yeoung Min;Cho, Namkyung;Kim, Seung-Han;Yu, Byungil;Lee, Kwang-Jin;So, Younseok;Woo, Seongphil;Im, Ji-Hyuk;Hwang, Chang Hwan;Lee, Jungho;Kim, Jin-han
    • Journal of the Korean Society of Propulsion Engineers
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    • v.22 no.3
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    • pp.109-118
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    • 2018
  • Korea Aerospace Research Institute has developed a staged combustion cycle rocket (SCCR) engine with high specific impulse to send a 3-ton class satellite into geostationary orbit while conducting a Korean Space Launch Vehicle (KSLV) II project. The SCCR engine is different from the KSLV-II engine, which is an open cycle engine using a gas-generator. The SCCR engine with a closed cycle engine is composed of a pre-burner, a turbo pump, and a main combustor. The technology demonstration model (TDM0) was assembled and tested in the 7ton-class engine combustion test facility of Naro Space Center, and the combustion test was successfully conducted.

Analysis for Case Study of Quality Management System to Solve Corporate Problems (기업의 문제해결을 위한 품질경영시스템 적용사례 분석)

  • Seo, Suwon;Jeong, Jongpil
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.18 no.4
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    • pp.191-203
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    • 2018
  • Identify the effects of ISO quality management system on the durability of the enterprise by applying it to early startups and small - and medium-sized enterprises. In particular, the quality management system of a technology start-up small and medium sized companies is important in applying the management system to the financial, management and quality control services rather than to research and development. The quality management system is applied to the medical equipment manufacturers, mobile phone brokers, foreign Chinese companies, and components manufacturers for diesel engines.Try it. Check the case where the quality management system has been applied to the company concerned and the nonconformities have been improved. In addition, the success of the project was determined by applying the quality control system to maintain the stability and perpetuation of the company through the case of the failed selection. The company explains that recently, it must appoint internal auditors to carry out continuous improvement activities and that those who want the development and continuity of the company must reflect the quality management system.

Development of Satisfaction Evaluation Items for Degree-linked High Skills Meister Courses using the Delphi Method (Delphi 기법을 활용한 학위연계형 고숙련마이스터 과정의 만족도 평가 문항 개발)

  • Kim, Seung-Hee
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.20 no.5
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    • pp.163-173
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    • 2020
  • In this study, on-site corporate instructors participated as student-cum-workers in a degree-linked high skills Meister course to improve job competency and practical ability as proposed in the Work-Study Career Vision. Evaluation questions were then developed and their validity was verified by assessing satisfaction related to expected goals in enhancing advanced training guidance and competency as an evaluator. Satisfaction assessment was conducted based on training preparation, training implementation, training effectiveness and training administration. The Delphi Method was adopted and a total of 48 items were developed in 6 categories under 4 main areas. There were 7 evaluation items on the satisfaction of training course development under training preparation, 21 evaluation items related to the satisfaction of Off-JT and OJT courses under training implementation, 16 evaluation items related to the satisfaction of increased competency as an on-site corporate instructor and the satisfaction of enhanced practical skills and skills application at work under training effectiveness, as well as 6 evaluation items to assess satisfaction with administrative support under training administration. The final conformity assessment was conducted based on the stability, content validity ratio, consensus and convergence indicators of the developed items. Results of this study do not only apply to quality management of the high skills Meister course which is being promoted as a pilot project for work-study programs, but also serves as a rationale that may be considered as a basic research tool in the collection of various opinions to derive overall system improvement factors for the work-study high skills Meister course.

Identification of cis-Regulatory Region Controlling Semaphorin-1a Expression in the Drosophila Embryonic Nervous System

  • Hong, Young Gi;Kang, Bongsu;Lee, Seongsoo;Lee, Youngseok;Ju, Bong-Gun;Jeong, Sangyun
    • Molecules and Cells
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    • v.43 no.3
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    • pp.228-235
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    • 2020
  • The Drosophila transmembrane semaphorin Sema-1a mediates forward and reverse signaling that plays an essential role in motor and central nervous system (CNS) axon pathfinding during embryonic neural development. Previous immunohistochemical analysis revealed that Sema-1a is expressed on most commissural and longitudinal axons in the CNS and five motor nerve branches in the peripheral nervous system (PNS). However, Sema-1a-mediated axon guidance function contributes significantly to both intersegmental nerve b (ISNb) and segmental nerve a (SNa), and slightly to ISNd and SNc, but not to ISN motor axon pathfinding. Here, we uncover three cis-regulatory elements (CREs), R34A03, R32H10, and R33F06, that robustly drove reporter expression in a large subset of neurons in the CNS. In the transgenic lines R34A03 and R32H10 reporter expression was consistently observed on both ISNb and SNa nerve branches, whereas in the line R33F06 reporter expression was irregularly detected on ISNb or SNa nerve branches in small subsets of abdominal hemisegments. Through complementation test with a Sema-1a loss-of-function allele, we found that neuronal expression of Sema-1a driven by each of R34A03 and R32H10 restores robustly the CNS and PNS motor axon guidance defects observed in Sema-1a homozygous mutants. However, when wild-type Sema-1a is expressed by R33F06 in Sema-1a mutants, the Sema-1a PNS axon guidance phenotypes are partially rescued while the Sema-1a CNS axon guidance defects are completely rescued. These results suggest that in a redundant manner, the CREs, R34A03, R32H10, and R33F06 govern the Sema-1a expression required for the axon guidance function of Sema-1a during embryonic neural development.

Physical and Chemical Properties of Waste Concrete Powders Originated from the Recycling Process of Waste Concrete (폐콘크리트의 재활용 공정에서 발생되는 폐콘크리트 미립분의 물리.화학적 특성)

  • Kim, Jin Man;Kang, Cheol;Kim, Ha Suk
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.4 no.2
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    • pp.82-89
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    • 2009
  • According to the great city development and the rapid growth of redevelopment project, waste concrete emission has been increased. Waste concrete powder is one of the by-product originated from the recycling of the waste concrete. The more making high quality recycled aggregate to use aggregate for concrete, the more waste concrete powder is producted relatively. Therefore, to realize the total recycling of waste concrete, development of recycling technology for waste concrete powder need very much. This technical note present the discharged process and the various properties of waste concrete powder. As the results, on the average, the maximum particle-size of waste concrete powder is less than $600{\mu}m$, and oven-dry density is less than $2.5g/cm^3$. And waste concrete powder contains more than 50% of $SiO_2$, 30% of CaO and 10% of $Al_2O_3$. Thus qualities of waste concrete powder is lower than those of high quality raw material for concrete. However, if it is processed by grading to the purpose, it will be used as resource of raw materials for construction field.

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The Design and Implementation of Ontology for Simulation based Architecture Framework (ONT-AF) in Military Domain (SBA AF의 구축을 지원하는 온톨로지의 설계 및 구현(ONT-SAF))

  • Kwon, Youngmin;Sohn, Mye;Lee, Wookey
    • Journal of Information Technology and Architecture
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    • v.9 no.3
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    • pp.233-241
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    • 2012
  • Architecture framework (AF) is a guideline to define components needed to develop and operate enterprise architecture (EA), and to define relationships among the components. There are many architecture frameworks to operate EA of governments and businesses such as Zachman framework, DoDAF, TOGAF, FEAF, and TEAF. DoDAF is the most representative AF to support the development of the EA in the military domain. DoDAF is composed of eight viewpoints and 40 views that are affiliated with the viewpoints. To develop an AF for a specific goal, system architects decide a set of views. Furthermore, they determine data that are needed for a view modeling. However, views and data in DoDAF are structurally inter-related explicitly and/or implicitly. So, developing an AF for a specific goal is going to be a project to be carried out over a long haul. To reduce the burden of its development, in this paper, we develop ONT-SAF (Ontology for DoDAF) that can infer inter-relationships like referential and transitive relationships and the sequences among the views. Furthermore, to promote reusability and consistency of the views and the data within an AF, we adopt the view-data separation strategy. ONT-DAT contains classes like 'viewpoint', 'view', 'data', 'expression method', and 'reference model', and 11 properties including 'hasView.' To prove the effectiveness of ONT-SAF, we perform a case study.

A Structured Methodology with Device Collaboration Diagram for Evaluating Context-Aware Systems (장비협업도를 활용한 상황인식 시스템에 대한 구조적 평가 방법론)

  • Kwon, Oh-Byung;Lee, Nam-Yeon
    • Journal of Intelligence and Information Systems
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    • v.13 no.2
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    • pp.27-41
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    • 2007
  • Nowadays the context-aware systems have been regarded as a promising opportunity to create differentiated e-marketplaces. Context-aware system aims to provide personalized services by understanding the user's current situation which is automatically acquired from the context data. This aim naturally leads us to a motivation to evaluate to what extent a system is context-aware. Even though lots of endeavors have stated about the level of context-aware system, a structured evaluation has been so far very rare. Hence, the purpose of this paper is to propose a two-phased methodology for assessing context-aware systems. In the first phase, we perform a requisite analysis to discriminate a context-aware system from general or context-based systems. Once an information system is recognized as context-aware system, then level of collaboration, mobility and embeddedness is derived to determine the level of context-aware system in the second phase. To do so, device collaboration diagram (DCD) is proposed to visualize the system architecture. Moreover, readiness and level of system are Jointly considered in the phase to provide a development strategy for each context-aware system development project. To show the feasibility of the idea proposed in this paper, legacy context-aware systems are actually analyzed and evaluated.

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Implementation of Efficient Container Number Recognition System at Automatic Transfer Crane in Container Terminal Yard (항만 야드 자동화크레인(ATC)에서 효율적인 컨테이너번호 인식시스템 개발)

  • Hong, Dong-Hee
    • Journal of the Korea Society of Computer and Information
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    • v.15 no.9
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    • pp.57-65
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    • 2010
  • This paper describes the method of efficient container number recognition in colored container image with number plate at ATC(Automatic Transfer Crane) in container terminal yard. At the Sinseondae terminal gate in Busan, the container number recognition system is installed by "intelligent port-logistics system technology development", that is government research and development project. It is the method that it sets up the tunnel structure inside camera on the gate and it recognizes the container number in order to recognize the export container cargo automatically. However, as the automation equipment is introduced to the container terminal and the unmanned of a task is gradually accomplished, the container number recognition system for the confirmation of the object of work is required at ATC in container terminal yard. Therefore, the container number recognition system fitted for it is necessary for ATC in container terminal yard in which there are many intrusive of the character recognition through image including a sunlight, rain, snow, shadow, and etc. unlike the gate. In this paper, hardware components of the camera, illumination, and sensor lamp were altered and software elements of an algorithm were changed. that is, the difference of the brightness of the surrounding environment, and etc. were regulated for recognize a container number. Through this, a shadow problem, and etc. that it is thickly below hung with the sunlight or the cargo equipment were solved and the recognition time was shortened and the recognition rate was raised.