• Ocean and Polar Research
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• v.39 no.1
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• pp.1-11
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• 2017

The aim of this study was to determine the physiological response and the applicable concentration ranges of anesthetic clove oil and anesthetic lidocaine-HCl, and to investigate the synergistic effect of a mixture of these two anesthetics on the in grass puffer (Takifugu niphobles). The anesthesia times decreased and the recovery times increased with increasing concentrations of clove oil and lidocaine-HCl. Applicable concentration ranges for long-term transportation requiring more than 1 hour were 2 ppm for clove oil and 50 ppm for lidocaine-HCl. With mixtures of the two anesthetics, the anesthesia time decreased as the admixture concentration of clove oil and lidocaine-HCl increased. Anesthesia times of experimental groups with the combined anesthetics were shorter than those with the same concentrations of clove oil or lidocaine-HCl alone. Plasma cortisol concentrations were highest at 6 hours in all experimental groups anesthetized with the mixture of clove oil and lidocaine-HCl, while all groups with clove oil or lidocaine-HCl alone had the highest plasma cortisol concentrations at 12 hours. Plasma glucose concentrations were highest at 12 hours in experimental groups anesthetized with the mixture of clove oil and lidocaine-HCl, while groups with clove oil or lidocaine-HCl alone had the highest plasma glucose at 24 hours. The results of this study provide basic information about anesthetics and the synergistic effect of mixtures of anesthetics in this fish species. This information should be useful for aquaculturists who require methods for safe and easy fish handling, and for transporters who require that minimal stress is imposed on fish during transport.

• Journal of the Korean Society of Marine Environment & Safety
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• v.17 no.1
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• pp.15-21
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• 2011

Species composition and seasonal variation of fish assemblages in Sargassum beds in Gamak Bay was determined using fish samples collected monthly by a surrounding net from October 2008 to September 2009. A total of 21 species, 582 individuals and 5,345.1 g of fishes were collected during the study period and Perciformes accounted for 47.6 % of the total number of species. Takifugu niphobles, Engraulis japonicus and Lateolabrax japonicus predominated in number, accounting for 55 % of the total number of individuals. Higher number of species and individuals occurred in June and in September-October. Species were grouped into three by cluster analysis: A) dominant species such as Leiognathus nuchalis and Takifugu niphobles, B) fish collected mainly in spring such as Sebastes inermis, Chaenogobius heptacanthus, Pterogobius zonoleucus, Pholis nebulosa, and Trachyrhamphus serratus, and C) fish collected mainly in autumn such as Engraulis japonicus, Lateolabrax japonicus, and Hyporhamphus sajori.

• Korean Journal of Ichthyology
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• v.23 no.3
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• pp.225-233
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• 2011

This is the attempt to study the fish assemblages in eelgrass bed of Jeogu on Geoje Island, Korea. Samples were collected by surf net monthly from August 2009 to July 2010. A total 38 species, 2,335 individuals, and 5,289.8g of fishes were collected. The dominant species were Chaenogobius annularis, Gymnogobius heptacanthus, Rudarius ercodes, Siganus fuscescens, Ditrema temminckii, Takifugu niphobles, Aulichthys japonicus, Pseudoblennius cottoides, Syngnathus schlegeli, Sebastes schlegelii, Pterogobius elapoides, Urocampus nanus, Takifugu pardalis, Hypodytes rubripinnis which accounted for 94.2% of total number of individuals of fish collected. Eelgrass biomass showed a peak in August (DW 121.5g/m$^2$), low values in November (DW 74.9 g/m$^2$) to February (DW 49.3g/m$^2$), and started growing from May (DW 112.8g/m$^2$). Gymnogobius heptacanthus, Sebastes schlegeli, Takifugu niphobles, Syngnathus schlegeli, Urocampus nanus were collected year-round with no clear relationship to the biomass of eelgrass bed.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.46 no.6
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• pp.725-732
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• 2013

To identify the food component characteristics of seven edible pufferfishes (five wild pufferfishes including striped puffer Takifugu xanthopterus; eyespot puffer Takifugu chinensis; purple puffer Takifugu porphyreus; rough-backed puffer Lagocephalus wheeleri; and grass puffer Takifugu niphobles; and two cultured pufferfishes including yellow puffer Takifugu obscurus, and tiger puffer Takifugu rubripes) in Korea, the proximate, fatty/amino acid, chemical and taste compositions were investigated. The proximate compositions were not significantly different among the sampled pufferfishes, whereas grass puffer had lower moisture and crude lipid levels contents, and higher crude protein and ash contents than the other species. The total amino acid levels in wild and cultured pufferfishes were 14,941.6-16,427.9 mg/100 g, and the major amino acids were aspartic acid, glutamic acid, glycine, alanine, leucine, lysine and arginine. Regarding the fatty acid and mineral compositions, the major fatty acids included 22:6n-3, 16:0, 18:1n-9, 20:5n-3, 18:0, 20:4n-3, and 22:5n-3 in that order, and there was a little difference among the sampled pufferfishes. Grass puffer had a higher monoenes ratio, i.e., 16:1n-7 and 18:1n-9, and a lower polyenes ratio than the other pufferfishes. The main minerals detected K, P, Na, S and Ca. Regarding the taste-active compounds, the free amino acid contents of pufferfish extracts were 236.4-428.1 mg/100 g, consisting primarily of taurine, lysine, proline, glycine, alanine and arginine in that order. The amino nitrogen content of the pufferfishes was 84.5-156.4 mg/100 g, and there was a little difference among the species. As for taste intensity, the total taste value of the purple puffer was higher than that of the other pufferfishes.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.5
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• pp.612-617
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• 2008

Tetrodotoxin (TTX) analogues were first determined from the liver extracts of puffer fish, Takifugu niphobles, by LC/MS with Hydrophilic Interaction Liquid Chromatography (HILIC). In total, 7 TTX analogues were detected within 20 minutes as follows; 5,6,11-trideoxyTTX (34.0%, 1,029.6 nmol/g), 6,11-dideoxyTTX (29.3%, 887.6 nmol/g), TTX (22.1%, 667.8 nmol/g), 4,9-anhydro-TTX (11.2%, 339.3 nmol/g), 11-deoxyTTX＋5-deoxyTTX (2.6%, 78.6 nmol/g), and 4-epiTTX (0.8%, 23.6 nmol/g). Mouse toxicity of diluted liver extracts showed the highest toxicity at pH 3 (8.7 MU/mL) and decreased, as increasing pH, to 1.4 MU/mL at pH 10. At acidic (pH 5) and neutral conditions (pH 7), mouse toxicity of liver extracts (79 MU/mL) decreased slowly, as increasing temperature from $80^{\circ}C$ to $115^{\circ}C$, and time until 1 hour; in contrast, at the akaline condition (pH 9), the toxicity decreased rapidly to the more than half within 10 minutes. Individual toxicity of the fillet of T. niphobles were between $43.2{\sim}106.7$ MU, and $64{\sim}78%$ of its toxicity was eluted to soup when boiled with 3 volumes of water during 10 minutes.

• Journal of agriculture & life science
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• v.43 no.6
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• pp.95-103
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• 2009

To develop natural intermediate flavoring substances, optimal hydrolysis conditions and taste compounds for two step enzyme hydrolysate(TSEH) using Grass puffer(Takifugu niphobles) were investigated. The optimal conditions for TSEH method were revealed in temperature at $55^{\circ}C$ 3 hour digestion with Alcalase (pH 7.5) at the 1st step and 2 hour at $45^{\circ}C$ digestion with Flavourzyme(pH 6.0~6.5) at the 2nd step. TSEH method was superior to hot-water extraction on the aspect of yield, nitrogen contents and organoleptic taste quality such as umami and control of bitter taste formation. In taste active-components in Glass puffer TSEH, total free amino acid content was 4,502 mg%, major free amino acids were Pro, Leu, Lys, Hypro, Tau, Arg, Phe, Ala, Glu and Val in ordor. As for nucleotides, IMP(372 mg%) was the principal component and also contents of TMAO, total creatinine, and betaine were 43, 278 and 41 mg% in Glass puffer TSEH, respectively. The major inorganic ions in TSEH were Na(949 mg%), K(222 mg%), Cl(1,180 mg%) and $PO_4$(1,081 mg%).

• Development and Reproduction
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• v.23 no.4
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• pp.367-375
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• 2019

Pufferfish (Takifugu spp.) are economically important edible marine fish. Mistakes in pufferfish classification can lead to poisoning; therefore, accurate species identification is critical. In this study, we used the mtDNA cytochrome c oxidase subunit I gene (COI) to design specific primers for six Takifugu species among the 21 domestic or imported pufferfish species legally sold for consumption in Korea. We rapidly and simultaneously identified these pufferfish species using a highly efficient, multiplex polymerase chain reaction (PCR) system with the six species-specific primers. The results showed that species-specific multiplex PCR (multiplex species-specific polymerase chain reaction; MSS-PCR) either specifically amplified PCR products of a unique size or failed. MSS-PCR yielded amplification fragment lengths of 897 bp for Takifugu pardalis, 822 bp for T. porphyreus, 667 bp for T. niphobles, 454 bp for T. poecilonotus, 366 bp for T. rubripes, and 230 bp for T. xanthpterus using the species-specific primers and a control primer (ca. 1,200 bp). We visualized the results using agarose gel electrophoresis to obtain accurate contrasts of the six Takifugu species. MSS-PCR analysis is easily performed and provides identification results within 6 h. This technique is a powerful tool for the discrimination of Takifugu species and will help prevent falsified labeling, protect consumer rights, and reduce the risk of pufferfish poisoning..

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2003.05a
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• pp.91-92
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• 2003

복어는 tetrodotoxin이라는 맹독을 함유하고 있음에도 불구하고 매운탕등 여러 가지로 요리하여 많은 사람들이 즐겨 먹는 음식의 하나이다. 복어 요리시에는 독을 함유한 내장이나 간장을 제외하고 세척하여 독을 제거하지만 조리과정에서 독이 완전히 제거되지 않거나 독성이 강한 복어의 경우는 독소가 남아 있어 식중독의 원인이 된다. 최근 부산에서는 2건의 복어요리에 의한 중독으로 수명의 중독환자가 발생하는 사고가 발생하였는바 다행히 사망하지는 않았으나, 복어요리는 항상 식중독의 위험성이 있음을 보여주고 있다. (중략)

• Journal of fish pathology
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• v.23 no.1
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• pp.107-112
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• 2010

Parasitism of Pseudocaligus sp. in wild grass puffer Takifugu niphobles in the coast of Yeosu in spring, 2007 was identified. The preadult and adult stages of Pseudocaligus sp. were observed, which consisted of cephalothorax, genital segment and abdomen. Lunules were present on the anterior margin of the frontal plate. In order to develop the treatment method, we examined the effects of formalin and fresh water on the parasite. The Pseudocaligus sp. were detached from the host fish by bathing at concentrations of 50 to 400 ppm formalin within 60 min, but 57% was exterminated to fresh water for 90 min.

• Korean Journal of Ichthyology
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• v.27 no.3
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• pp.233-237
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• 2015

Fish assemblages in the coastal waters off Gampo in the East Sea of Korea were analyzed using the samples of fishes collected with pot nets June, September, and December 2005 and March 2006. During the study period, a total of 19 fish species representing 10 families in 4 orders were collected. The fish assemblages predominantly consisted of Conger myriaster, which accounted for 38.9% of the number of individuals and 52.2% in the biomass. The next major specimens were Takifugu niphobles, Sebastes schlegelii, Chaenogobius annularis, Hexagrammos agrammus and Hexagrammos otakii. These five fishes accounted 44.1% of total number of individuals and 37.3% of total biomass. The number of fish species was the highest in March 2006 and the lowest in December 2005. The number of individuals and biomass were the highest in September in 2005 and the lowest in December in 2005.